Abstract
The physiological and biochemical behaviour of rice (Oryza sativa, var. Jyoti) treated with copper (II) oxide nanoparticles (CuO NPs) was studied. Germination rate, root and shoot length, and biomass decreased, while uptake of Cu in the roots and shoots increased at high concentrations of CuO NPs. The accumulation of CuO NPs was observed in the cells, especially, in the chloroplasts, and was accompanied by a lower number of thylakoids per granum. Photosynthetic rate, transpiration rate, stomatal conductance, maximal quantum yield of PSII photochemistry, and photosynthetic pigment contents declined, with a complete loss of PSII photochemical quenching at 1,000 mg(CuO NP) L−1. Oxidative and osmotic stress was evidenced by increased malondialdehyde and proline contents. Elevated expression of ascorbate peroxidase and superoxide dismutase were also observed. Our work clearly demonstrated the toxic effect of Cu accumulation in roots and shoots that resulted in loss of photosynthesis.
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Abbreviations
- AAS:
-
atomic absorption spectrophotometer
- APX:
-
ascorbate peroxidase
- DM:
-
dry mass
- E :
-
transpiration rate
- FM:
-
fresh mass
- Fm :
-
maximum fluorescence
- Fo :
-
initial fluorescence
- Fs :
-
steady-state fluorescence
- Fv/Fm :
-
maximal quantum yield of PSII photochemistry
- GR:
-
glutathione reductase
- gs :
-
stomatal conductance
- IRGA:
-
infra red gas analyser
- MDA:
-
malondialdehyde
- NP(s):
-
nanoparticle(s)
- P N :
-
photosynthetic rate
- qP :
-
photochemical quenching
- ROS:
-
reactive oxygen species
- SEM:
-
scanning electron microscope
- SOD:
-
superoxide dismutase
- TBA:
-
thiobarbituric acid
- TEM:
-
transmission electron microscope
- XRD:
-
X-ray diffraction
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Acknowledgements: We thank the Department of Science & Technology (DST), New Delhi (SR/SO/PS-63/2009) and UGC-SAP (F. 3-50/2009 (SAP-II) for funding this work. We would like to thank All India Institute of Medical Sciences, New Delhi for TEM imaging; NIO, Goa for SEM imaging and atomic absorption spectrophotometry; Physics Department, Goa University for NP size determination using X-ray diffractometer. We are grateful to Andrew Willis, Centre for Agroecology, Water and Resilience (CAWR), Coventry University, Coventry, United Kingdom for correcting the English of the manuscript.
Both authors made equal contribution to the work presented in this paper.
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Da Costa, M.V.J., Sharma, P.K. Effect of copper oxide nanoparticles on growth, morphology, photosynthesis, and antioxidant response in Oryza sativa . Photosynthetica 54, 110–119 (2016). https://doi.org/10.1007/s11099-015-0167-5
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DOI: https://doi.org/10.1007/s11099-015-0167-5