Background. Brain histaminergic neurons play an important role in regulating many body functions, systems, and reactions, and also in the pathogenesis of many pathological states and diseases. Histamine in the brain functions as a neurotransmitter and is located mainly in histaminergic neurons. All histaminergic neurons in the hypothalamus, in contrast to other types of neurons, have high monoamine oxidase type B (MAO B) activity, this being a key enzyme in histamine metabolism in the brain. Objective. Parallel assessment of MAO B activity and immunoreactivity in histaminergic neurons in the rat hypothalamus during postnatal ontogeny. Materials and methods. Experiments were run on hypothalamus specimens from 5-, 10-, 20-, 45-, and 90-day offspring of white rats (45 rat pups) in compliance with the “Guidelines for Studies Using Experimental Animals.” Hypothalamus sections were processed histochemically to detect MAO B activity and immunohistochemically using antibodies to MAO B. Results. MAO B, the enzyme catalyzing the oxidative deamination of histamine and functioning as a marker enzyme for histaminergic neurons in the hypothalamus, showed no cytoplasmic activity or immunoreactivity on day 5 after birth, after which these measures increased in parallel from day 10 to day 90 of postnatal ontogeny. Conclusions. The synchronicity of postnatal development of the MAO B activity and immunoreactivity in histaminergic neurons in the brain is evidence of the parallel accumulation of MAO B protein in these cells and increases in its enzymatic activity, reflecting the establishment of their specific neurotransmitter metabolism.
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Translated from Morfologiya, Vol. 159, No. 1, pp. 13–19, January–February, 2021.
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Zimatkin, S.M., Zaerko, A.V. Monoamine Oxidase B in Developing Histaminergic Neurons in the Rat Brain. Neurosci Behav Physi 52, 953–956 (2022). https://doi.org/10.1007/s11055-022-01319-y
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DOI: https://doi.org/10.1007/s11055-022-01319-y