The effects of intranasal administration of oxytocin and thyroliberin solutions on changes in behavior in rats after moderate social stress were studied. Investigations were carried out on male Wistar rats (n = 100). Animals of experimental group 1 received intranasal oxytocin at a dose of 0.25 IU in 20 μl bilaterally and those of experimental group 2 received thyroliberin at a dose of 10 10 mmol in 10 μl, while control animals received the same volume of physiological saline. Animals were exposed to moderate social stress for 1 h starting 15 min after substance administration. Animals were tested in an elevated plus maze (EPM) after a further 3 h. These experiments showed that administration of oxytocin led to a decrease in the level of anxiety in social stress as compared with controls. Administration of thyroliberin had no effect on stress-induced changes in behavioral parameters.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Ashmarin, I. P., Kulaichev, A. P., and Chepurnov, S. A., “Cascade undirected processes controlled by short-lived peptides (thyroliberin),” Ros. Fiziol. Zh. im. I. M. Sechenova, 75, No. 5, 627–632 (1989).
Batuev, A. S., Polyakova, O. N., and Aleksandrov, A. A., “Effects of ‘social’ stress of during pregnancy in rats on the level of anxiety in the offspring,” Zh. Vyssh. Nerv. Deyat. I. P. Pavlova, 50, No. 2, 281–286 (2000).
Vinogradova, E. P. and Chaadaeva, E. V., “Changes in the level of anxiety in female white rats during the estral cycle and depending on handling,” Zh. Vyssh. Nerv. Deyat. I. P. Pavlova, 44, No. 2, 277–282 (1994).
Vinogradova, E. L., Kargin, A. V., Zhukov, D. A., and Markov, A. G., “Effects of thyroliberin on the behavioral component of the stress response in Wistar rats,” Zh. Vyssh. Nerv. Deyat. I. P. Pavlova, 64, No. 6, 660–667 (2014).
Grigor’yan, G. A. and Gulyaeva, I. V., “Stress reactivity and stress resistance in the pathogenesis of depressive disorders: the role of epigenetic mechanisms,” Zh. Vyssh. Nerv. Deyat. I. P. Pavlova, 65, No. 1, 19–32 (2015).
Kalinina, V. V., Ivannikova, N. O., Koplik, E. V., Smolila, N. V., Gryzunov, Yu. A., and Dobretsov, G. E., “Effects of stress on the development of hemorrhagic stroke,” Zh. Vyssh. Nerv. Deyat. I. P. Pavlova, 62, No. 4, 506–512 (2012).
Kovalenko, R. I., Sibarov, D. A., Pavlenko, I. N., and Luk’yanova, E. L., “Structure of pinealocytes in rats in stress and after unilateral intrana sal administration of oxytocin,” Ros. Fiziol. Zh. im. I. M. Sechenova, 8, 87–93 (1997).
Kudryashova, I. V. and Gulyaeva, N. V., “’Unpredictable stress’: the heterogeneity of stress reactivity in studies of long-term plasticity,” Zh. Vyssh. Nerv. Deyat. I. P. Pavlova, 66, No. 4, 414–428 (2016).
Chepurnov, S. A., Chepurnova, N. E., Abbasova, K. R., and Goncharov, O. B., “The neuropeptide thyroliberin - an endogenous anticonvulsant in the brain,” Usp. Fiziol. Nauk., 33, 29–39 (2002).
Bartz, J. A., Zaki, J., Bolger, N., and Ochsner, K. N., “Social effects of oxytocin in humans: context and person matter,” Trends Cogn. Sci., 15, No. 7, 301–309 (2001).
Campbell, A., “Oxytocin and human social behavior,” Pers. Soc. Psychol. Rev., 14, 281–295 (2010).
Carter, C. S. and Altemus, M., “Integrative functions of lactational hormones in social behavior and stress management,” Ann. N.Y. Acad. Sci., 807, 164–174 (1997).
Carter, C. S., Boone, E. M., and Bales, K. L., “Early experience and the developmental programming of oxytocin and vasopressin,” in: Neurobiology of the Parental Brain, Bridges, R. S. (ed.) Elsevier, San Diego (2008), pp. 417–433.
Donaldson, Z. R. and Young, L. J., “Oxytocin, vasopressin, and the neurogenetics of sociality,” Science, 322, No. 5903, 900–904 (2008).
Engelmann, M., Landgraf, R., and Wotjak, C. T., “The hypothalamic-neurohypophysial system regulates the hypothalamic-pituitary-adrenal axis under stress: an old concept revisited,” Front. Neuroendocrinol., 25, 132–149 (2004).
Gutiérrez-Mariscal, M., de Gortari, P., López-Rubalcava, C., Martinez, A., and Joseph-Bravo, P., “Analysis of the anxiety-like effect of TRH and the responses of amygdalar TRHergic neurons in anxiety,” Psychoneuroendocrinology, 33, No. 2, 198–313 (2008).
Haller, J., Aliczki, M., and Gyimesine Pelczer, K., “Classical and novel approaches to the preclinical testing of anxiolytics: A critical evaluation,” Neurosci. Biobehav. Rev., 37, No. 10, Part 1, 2318–2330 (2013).
Heinrichs, M., Meinlschmidt, G., Wippich, W., Ehlert, U., and Hellhammer, D. H., “Selective amnesic effects of oxytocin on human memory,” Physiol. Behav., 83, No. 1, 31–38 (2004).
Hollander, E., Bartz, J., Chaplin, W., Phillips, A., Sumner, J., Soorya, L. et al., “Oxytocin increases retention of social cognition in autism,” Biol. Psychiatry, 61, No. 4, 498–503 (2007).
Jaworska-Feil, L., Kajta, M., Budziszewska, B., Leskiewicz, M., and Lason, W., “Protective effects of TRH and its stable analogue, RGH-2202, on kainate-induced seizures and neurotoxicity in rodents,” Epilepsy Res., 43, 67–73 (2001).
Kent, P., Awadia, A., Zhao, L., Ensan, D., Silva, D., Cayer, C., James, J. S., Anisman, H., and Merali, Z., “Effects of intranasal and peripheral oxytocin or gastrin-releasing peptide administration on social interaction and corticosterone levels in rats,” Psychoneuroendocrinology, 64, 123–130 (2015).
Knoblach, S. M. and Kubek, M. J., “Thyrotropin-releasing hormone release is enhanced in hippocampal slices after electroconvulsive shock,” J. Neurochem., 62, 119–125 (1994).
Kosfeld, M., Heinrichs, M., Zak, P. J., Fischbacher, U., and Fehr, E., “Oxytocin increases trust in humans,” Nature, 435, 673–676 (2005).
Kumsta, R. and Heinrichs, M., “Oxytocin, stress and social behavior: neurogenetics of the human oxytocin system,” Curr. Opin. Neurobiol., 23, 11–16 (2013).
Landgraf, R. and Neumann, I. D., “Vasopressin and oxytocin release within the brain: a dynamic concept of multiple and variable modes of neuropeptide communication,” Front. Neuroendocrinol., 25, 150–176 (2004).
Lloyd, R. L., Pekary, A. E., Sattin, A., and Amundson, T., “Antidepressant effects of thyrotropin-releasing hormone analogues using a rodent model of depression,” Pharmacol. Biochem. Behav., 70, No. 1, 15–22 (2001).
Neumann, I. D., “Involvement of the brain oxytocin system in stress coping: interactions with the hypothalamo-pituitary-adrenal axis,” Prog. Brain Res., 139, 147–162 (2002).
Neumann, I. D., “Brain oxytocin: a key regulator of emotional and social behaviours in both females and males,” J. Neuroendocrinol., 20, No. 6, 858–865 (2008).
Ogawa, N., Mizuno, S., Mori, A., Nukina, L., Ota, Z., and Yamamoto, M., “Potential anti-depressive effects of thyrotropin releasing hormone (TRH) and its analogues,” Peptides, 5, 743–746 (1984).
Palgi, S., Klein, E., and Shamay-Tsoory, S. G., “Oxytocin improves compassion toward women among patients with PTSD,” Psychoneuroendocrinology, 64, 143–149 (2016).
Pellow, S., Chopin, P., File, S., and Briley, M., “Validation of open:closed arm entries in an elevated plus-maze as a measure of anxiety in the rat,” Neurosci. Methods, 14, 149–167 (1985).
Schally, A., “Aspects of hypothalamic regulation of the pituitary gland,” Science, 202, 18–28 (1978).
Shepherd, J. K., Grewal, S. S., Fletcher, A., Bill, D. J., and Dourish, C. T., “Behavioural and pharmacological characterisation of the elevated “zero-maze” as an animal model of anxiety,” Psychopharmacology (Berl.), 116, No. 1, 56–64 (1994).
Szuba, M. F., Amsterdam, J. D., Fernando, A. T. 3rd, Gary, K. A., Whybrow, P. C., and Winokur, A., “Rapid antidepressant response after nocturnal TRH administration in patients with bipolar type I and bipolar type II major depression,” J. Clin. Psychopharmacol., 25, No. 4, 325–330 (2005).
Unkelbach, C., Guastella, A. J., and Forgas, J. P., “Oxytocin selectively facilitates recognition of positive sex and relationship words,” Psychol. Sci., 19, 1092–1094 (2008).
Waldherr, M. and Neumann, I. D., “Centrally released oxytocin mediates mating-induced anxiolysis in male rats,” Proc. Natl. Acad. Sci. USA, 104, 16681–16684 (2007).
Wan, R. Q., Noguera, E. C., and Weiss, S. R., “Anticonvulsant effects of intra-hippocampal injection of TRH in amygdala kindled rats,” Neuroreport, 9, 677–682 (1998).
Winslow, J. T. and Insel, T. R. , “The social deficits of the oxytocin knockout mouse,” Neuropeptides, 36, No. 2–3, 221–229 (2002).
Veronesi, M. C., Kubek, D. J., and Kubek, M. J., “Intranasal delivery of a thyrotropin-releasing hormone analog attenuates seizures in the amygdale-kindled rat,” Epilepsia, 48, No. 12, 2280–2286 (2007).
Zeng, H., Schimpf, B. A., Rohde, A. D., Pavlova, M. N., Gragerov, A., and Bergmann, J. E., “Thyrotropin-releasing hormone receptor 1-deficient mice display increased depression and anxiety-like behavior,” Molec. Endocrinology, 21, No. 11, 2795–2804 (2007).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Zhurnal Vysshei Nervnoi Deyatel’nosti imeni I. P. Pavlova, Vol. 67, No. 3, pp. 341–348, May–June, 2017.
Rights and permissions
About this article
Cite this article
Vinogradova, E.P., Kargin, A.V., Ogienko, N.A. et al. Effects of Oxytocin and Thyroliberin on Anxiety in Male White Mice in Social Stress. Neurosci Behav Physi 48, 1019–1023 (2018). https://doi.org/10.1007/s11055-018-0664-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11055-018-0664-7