Western immunoblotting and immunohistochemistry methods were used to study the expression of hypoxia-inducible factor 1α (HIF-1α) in the brain of the mitten crab Eriocheir japonica in normal conditions and anoxia lasting 2, 4, 6, and 12 h using a water deprivation model. The number of immunopositive neurons was small in intact crabs, and increased with increases in the duration of anoxia. There was a particularly marked increase in the proportion of neurons expressing HIF-1α in cells of group 6. In group 9/11, the highest values were seen at 2–6 h of anoxia. In group 17, significant changes in the proportion of immunopositive cells were noted only at 2 h of anoxia. After anoxia lasting 6 h, the proportion of neurons expressing HIF-1α decreased in all cell groups, though the reaction appeared in hemocytes. It is suggested that the increase in the proportion of immunopositive neurons and the suppression of HIF-1α expression in brain hemocytes in anoxia play important roles in mediating compensatory and protective processes, increasing the adaptive potential of the mitten crab in conditions of hypoxic stress.
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Translated from Morfologiya, Vol. 149, No. 1, pp. 27–32 January–February, 2016.
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Chertok, V.M., Kotsyuba, E.P. Localization and Quantitative Assessment of Oxygen-Sensitive Hypoxia-Inducible Factor 1α in the Brain of the Mitten Crab Eriocheir Japonica in Normal Conditions and Acute Anoxia (an immunohistochemical study). Neurosci Behav Physi 47, 12–16 (2017). https://doi.org/10.1007/s11055-016-0360-4
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DOI: https://doi.org/10.1007/s11055-016-0360-4