Abstract
It has long been recognized that, among extant mammals, the afrotherian clade Tenrecomorpha contains an exceptional range of sensory specialists in which arboreal, fossorial, semiaquatic and possibly even echolocating species occur within a single clade. Despite their obvious interest in this regard, the sensory apparatus of these animals has not been investigated with modern techniques. Presented here is a geometric morphometric analysis of virtual endocasts of 24 tenrecomorph species (~ 69% of extant diversity) reconstructed via high-resolution uCT techniques. Utilizing linear regression and PCA analyses we identify a model including allometry, habitat, and evolutionary history as the main factors underlying shape variability. Distinct clusters in the tenrecomorph morphospace correspond to shifts within the olfactory and cortical regions of the brain, which covary with independent evolution of aquatic and fossorial behaviors. These results showcase remarkable instances of sensory convergence within the clade and provide a template for inter- and intra-clade analyses of this distinctive branch of the mammal tree.
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Introduction
The afrotherian mammal clade Tenrecomorpha contains a wide array of distinct sensory ecomorphotypes (Olson 2013; Everson et al. 2016), including arboreal, fossorial, semiaquatic, and even echolocating (Gould 1965) behaviors. These specializations are distributed among ~ 35 extant, narrowly distributed species, with contemporary phylogenies placing all in suborder Tenrecomorpha (Everson et al. 2016), containing the families Tenrecidae and Potamogalidae, the latter then result of elevating the former subfamily Potamogalinae to family status. Since their arrival on the island of Madagascar between 50–20 Ma (Ali and Huber 2010; Samonds et al. 2012; Everson et al. 2016), these insectivores have since evolved into a dazzling array of distinct ecological niches. However, the degree to which these adaptations are reflected in tenrecomorph anatomy remains controversial: previous studies on skull morphology (e.g., Finlay and Cooper 2015) have documented surprisingly little disparity within the clade (in large part due to similarities encountered in the most speciose genus, Microgale), while those focusing on the postcranial skeleton (Salton and Szalay 2004; Salton and Sargis 2008a, b, 2009) found that cladistic and ecomorphotype signals varied, depending on the specific skeletal components investigated.
Small mammals with similar sensory adaptations exhibit highly specialized internal sensory structures, such as trade-offs between visual and olfactory brain structures in bats and primates (Barton et al. 1995), well-developed trigeminal systems in semiaquatic mammals (Sánchez-Villagra and Asher 2002), and the size of the eyes, ethmoid, and tympanic membrane in various taxa across ecological niches (Nummela et al. 2013). Given the range of sensory adaptations evident in tenrecomorph species and the conflicting signals found in osteological studies, understanding the soft tissue structures underpinning function in ecomorphotypes would be an essential step in recovering not only tenrecomorph evolutionary history, but also the nature of convergent evolution of neurosensory structures (Sulser 2022). Should cranial anatomy vary according to ecomorphotype, and correspond to neuroanatomical trends identified in other groups (e.g., olfactory bulb reduction in aquatic mammals; Sánchez-Villagra and Asher 2002), then tenrecs would be expected to exhibit anatomical convergence with phylogenetically distant groups for such features and may utilize similar genomic and developmental pathways to produce these sensory specializations. Similarly, if signals of novel variation (or no signal at all) are recovered, then tenrecs might represent an exception to conclusions based on studies of brain shape determined primarily from non-afrotherian mammals, suggesting potential broad-scale clade distinctions within mammals.
Cranial endocasts represent an oft-utilized metric for the representation of brain shape (Watanabe et al. 2018; Dumoncel et al. 2021). While endocasts may not capture all aspects of neuroanatomical variation, particularly with respect to neural density and size, as in the case of cortical regions (Collins et al. 2010; Herculano-Houzel et al. 2015), they are nonetheless useful for evaluating sensory evolution in small mammals both extant (Ahrens 2014; Bertrand et al. 2019) and extinct (Silcox et al. 2010; Bertrand et al. 2018; Ni et al. 2019). Thus, anatomical description and intraclade comparison of tenrecomorph cranial endocasts may be helpful in shedding light on areas of potential functional relevance. Hereafter, the term “endocast” should be assumed to refer to volumetric representations of the interior of the brain case exclusively.
In this study, we investigate virtual endocasts collected from 24 taxa across Tenrecomorpha (Potamogalidae + Tenrecidae). We anatomically characterize each major subfamily, and use landmark-based geometric morphometrics (GM) (Zelditch et al. 2012), phylogenetic least-squares regression (PGLS) (Adams 2014a) and principal component analysis (PCA) to understand the nature of endocast variation within the tenrecomorph sensory system. We explicitly test the hypothesis that tenrecomorph sensory anatomy, as reflected in endocast shape, varies across and in correlation with sensory ecomorphotype.
Materials and methods
Specimens for this project were loaned from the mammalogy collections of the American Museum of Natural History (AMNH) and The Field Museum (FMNH) (see Table 1). All were scanned on the GE Phoenix Vtome x SMicroCT scanner housed in the AMNH Microscopy and Imaging Facility (MIF). Scans were performed with resolutions of 18–56 µm. Image stacks were generated and endocasts reconstructed utilizing Mimics software (Materialise Mimics 2020). Endocasts were segmented via a combination of Multiple Slice Edit and Threshold functions to select the “empty space”, i.e., volumes less dense than bone within the cranial cavity. The resulting segmented endocranial models were pruned by hand to ensure a solid continuous mesh prior to export for landmark collection. Three-dimensional landmarks were placed along the left and midline of the skull using Landmark Editor 2.7 (Wiley 2005) (Fig. 1, Table 2). All bilaterally symmetrical points were mirrored prior to general Procrustes analysis (GPA), as side-only landmark studies are known to contain errors (Cardini 2017). Landmarks were chosen based on their repeatability and relevance for outlining the margins of major brain regions (Ahrens 2014; Bertrand et al. 2018, 2019).
Morphometric analyses were performed in R (R Core Team 2013), utilizing the packages Geomorph (Adams and Otárola-Castillo 2013), RGL, and Paleomorph. General Procrustes analysis (GPA) (Zelditch et al. 2012) was performed in order to orient and scale all specimens prior to downstream analyses. In order to identify evolutionary signal, the K-statistic (Adams 2014b) was estimated from the full dataset utilizing physignal and a published phylogenetic tree (Everson et al. 2016). Procrustes ANOVA and PGLS (Adams and Collyer 2018) tests were run using the ProcD.Lm, ProcD.pgls and anova functions to explore the relationships between different potential covarying factors according to published literature (diet, ecomorphotype, habitat, and locomotion), in addition to the potential effects of allometry (as observed through log centroid size). A list of all factors analyzed and citations for each can be found in Online Resource 1. All permutations of PCA were performed utilizing gm.prcomp (Collyer and Adams 2021).
Results
After Procrustes alignment and ordination, testing via the K-statistic confirms the presence of significant evolutionary signal in the dataset (observed K = 0.681, P < 0.001, Fig. 2). The results of ANOVA regression and PGLS are available in Online Resource 2. In single-factor ANOVA analyses, ecomorphotype and diet explain the most variation, while in single-factor PGLS testing habitat was the sole statistically significant factor. When multiple factors are taken into account, a regression model including both ecomorphology and habitat was found to be the best fit (R-squared value ~ 43%, P > 0.001 under ANOVA; R-squared value ~ 30%, P > 0.002 under PGLS; see Online Resource 2). Given the highly variable per-taxon sample sizes in this study (e.g., few examples of extreme morphologies such as those exhibited by semiaquatic Potamogalidae vs. abundant and overlapping examples of speciose terrestrial insectivores within Microgale) means that PGLS results should be viewed with caution.
PCA results are shown in Figs. 3, 4, and 5. To better quantify shape variation attributed to phylogeny, the shape space was run under a GLS rotation (Collyer and Adams 2021) to visualize shape variation with minimal phylogenetic influence (Fig. 4). Phylogenetically aligned component analysis (PaCA) was also run, and results are available in Online Resource 3.
With respect to the groupings in the unadjusted PCA, the first component accounted for 31.75% of the variation in the dataset, with the second and third representing 15.44% and 11.11%, respectively (all other components each accounted for < 9% of the total variance) (Fig. 3). The sample mesh for the positive PC1 values reflects a quite compressed and rounded endocast, a markedly “pinched” hindbrain (neocortex higher than any lobe on the cerebellum). The piriform lobes are compressed mediolaterally yet expanded ventrally, whilst the negative extreme in PC1 presents anteroposterior elongation of the entire endocast in the morphospace. There is also a relatively long pons, medulla, and cerebellum with relatively small paraflocculi, as well as an expanded olfactory region. With respect to PC2, the positive axis represents large olfactory bulbs and mediolaterally expanded piriform lobes, all larger than the brainstem; the negative values represent exceptionally small olfactory bulbs, a drastically reduced anterior piriform cortex with an expanded neocortex (creating a sloped effect anteriorly), a widened hindbrain larger in mediolateral width than the olfactory bulbs, and a dorsoventrally compressed cerebellar region. The PC2 negative values also show that the exit apertures of the oval foramina for the mandibular nerve are closer together than on the positive axis and are compressed mediolaterally. Finally, the PC3 positive axis represents a smooth neocortex that is higher than any point on the cerebellum, with a dorsal expansion and mediolateral compression of the neocortex and piriform cortex. By contrast, the negative axis represents a marked “pinching” at the anterior margin of the cortices near the olfactory bulbs, with a corresponding mediolaterally expanded cerebellum that also exhibits a vermis higher than the neocortex.
Several patterns can be identified via the groups present within these first three PCA components. All semiaquatic taxa (Potamogale, Micropotamogale, and Microgale mergulus, yellow) group with one another, near the negative extreme of PC2, whilst arid-adapted tenrecines occupy a distinct region of the morphospace. The sole exception to this generalization, Hemicentetes semispinosus, occurs in mesic lowland habitats. Nearly all oryzorictines form a cluster with Hemicentetes, with three exceptions: Microgale mergulus and the two species of Nesogale. Comparison between PC1 and PC3 highlights the disparity found in the tenrecines (Hemicentetes in particular) and marks the distinctiveness of Nesogale in comparison to species of Microgale, which is in line with skeletal (Thomas 1918) and genetic (Everson et al. 2016) arguments for distinguishing these genera. In contrast, comparing PC2 with PC3 compresses all oryzorictines and Geogale into the center of the morphospace but highlights the degree to which Microgale mergulus uniquely clusters with the potamogalids and further underlines the separation of Hemicentetes from the rest of the Tenrecomorpha.
Remarkably, the PaCA morphospace (Online Resource 3) for these data neatly reinforces the findings in the unadjusted PCA, suggesting these first two principal components are related to evolutionary history and phylogeny. The minima and maxima for the first axis in both the PaCA and PCA analyses resolve similar brain shapes and, while the second axis flips the shapes assigned to minimum and maximum, the resulting phylomorphospaces are otherwise nearly identical. It is perhaps expected, then, that removing the phylogenetic signal (either utilizing transformed or untransformed projections; Collyer and Adams 2021) (Fig. 4c-d) collapses several of these components, with the PCA transformed projection clustering most taxa in the center of the morphospace. In this transformed morphospace, the first principal component is virtually unchanged, whereas the negative and positive axes of the second component of the transformed PCA portrays a minimum with a lengthened hindbrain and wider, flaring piriform lobes compared to a maximum value with smaller piriform lobes and a sloping neocortex – all of which yields a result remarkably similar to those produced in principal component 3 of the untransformed PCA.
Allometry was found to be statistically significant in our analyses of shape (R-squared value ~ 30%, P < 0.0004; Online Resource 2), and for this reason, the potential effects of size on the shape analyses recovered were also investigated. To account for allometry, all PCA plots were replicated on the residuals of a regression of shape against log centroid size (Fig. 4b, Online Resource S3). Minimizing the allometric component from these analyses drastically shifts the morphospace, as might be expected from the statistically significant relationship between size and shape recovered from ANOVA testing. When adjusted for this component, the semifossorial species in the genus Oryzorictes and Microgale gymnorhyncha cluster together with Hemicentetes semispinosus, while the Potamogalidae + M. mergulus cluster expands in morphospace occupation, with borders near the terrestrial and arboreal species of Microgale. Of particular note is the fact that the monotypic member of subfamily Geogalinae (Geogale aurita) occupies the minimum of both principal axes. On the whole, the adjusted component analysis (Fig. 4b) still reflects the phylogenetic and ecological signals observed in the other PCA, including a distinct region for arid-adapted tenrecines, tight clustering of the oryzorictines, and separate groupings among the semiaquatic taxa. Transforming the dataset to remove phylogenetic signal, however, results in an amorphous “cluster” of taxa with no clear groupings (Online Resource 3), a sign that no strong ecological signal occurs once the factors of phylogeny and allometry have been removed.
Cranial endocast renditions of representative samples for each clade analyzed are presented in Figs. 1 and 5, and Online Resources 4–7. Members of the clade Potamogalidae, represented here by Micropotamogale lamottei (Fig. 5a), exhibit drastically reduced olfactory bulbs and olfactory tubercles, albeit with an expanded neocortex and a flattened cerebellum with a distinctive “arrowhead” shape. By contrast, the sole species in the clade Geogalinae, Geogale aurita (Fig. 5b), displays extended and enlarged olfactory regions, which are angled and raised above the level of the cortices in lateral view. In keeping with the study by Stephan et al. (1991), the neocortex appears particularly reduced, but areas of the endocast corresponding to the piriform lobe are relatively robust. The cerebellum appears dorsoventrally flattened, with no rounded “pinching” or mediolateral extension noted in other similar-sized tenrecs. Clade Tenrecinae, exemplified by Echinops telfairi (Fig. 1) displays relatively large olfactory bulbs alongside reduced cortices and hindbrain. Hemicentetes semispinosus (Fig. 5c) is an exception within this clade, presenting instead relatively small olfactory bulbs and a bulging cerebellum. Finally, Oryzorictes hova is marked by relatively large olfactory bulbs, a “rounded” brain (including a flattened cerebellum with a vermis below the neocortex), and an extended piriform lobe that dips below the bottom of the endocast (Fig. 5d).
Discussion
When comparing the effects of size, shape, and ecomorphotype on the form of tenrecomorph endocasts, the influence of ecomorphotype is clearly evident. But it also is clear that evolutionary signal, allometry, and habitat all play a statistically significant role in shaping the tenrecomorph endocast, as supported by Kmult and ANOVA testing (see Online Resource 2). Furthermore, ANOVA tests and the results of morphospace adjustment indicate that these factors are not independent. Given that discernable clustering vanishes within the morphospace when evolutionary history and allometry are removed from the analysis, it is likely that these two factors are dominant in their effect on tenrecid endocranial variation. Ecomorphology and habitat certainly help explain the groupings observed in this analysis (and are statistically significant in the Procrustes ANOVA results) but may only provide a weak signal when assessed separately from evolutionary history and allometry as primary influences on clustering in endocast morphospace. The first principal component in the unaltered analysis, in particular, appears to be highly correlated with allometry. Within this sample, larger taxa show a lengthening of the olfactory bulbs and a pronounced vermis while smaller taxa exhibit wide petrosal lobule regions and a distinct “rounding” of the entire endocast (dorso-ventral expansion and rostral compression, with a short brainstem and flattened olfactory bulbs). Not all subfamilies display an equal amount of variation. Indeed, in keeping with previous morphometric analyses (Finlay and Cooper 2015), the genus Microgale is found to be relatively constrained morphologically, with the notable exception of M. mergulus. The tenrecines are responsible for driving an unexpectedly large amount of the variation observed across the entire tenrecid morphospace, even after accounting for allometry. The semiaquatic morphotype appears especially distinctive, as potamogalids and M. mergulus cluster together in nearly all analyses, even though they are widely separated phylogenetically. In agreement with the high degree of evolutionary signal observed, each subfamily has unique aspects with respect to their distribution within morphospace, as we now summarize.
Potamogalidae
Several studies (Sánchez-Villagra and Asher 2002; Salton and Sargis 2008b, 2009) have noted the effect of semiaquatic adaptations on morphology in this clade, influencing the foramen magnum, infraorbital foramen, the acetabulofemoral joint, and aspects of the pisiform. In this regard, the endocast proves no exception. Semiaquatic tenrecomorph taxa cluster near the higher end of PC2 in the unadjusted morphospace, in agreement with previous studies investigating the semiaquatic ecomorphotype of small mammals. In particular, reduced olfactory regions are consistent with other semiaquatic adaptations in the clade (Stephan et al. 1991). Reduced olfactory capabilities represent a trade-off in sensory specializations (perhaps in favor of somatosensation) in potamogalids, and their large hindbrain and brainstem may reflect an increased demand for fine motor control in navigating an aquatic environment. This clade is not immune to allometric adjustment, as removing the allometric component from this analysis (Fig. 5) expands the originally tightly clustered group to cover more of the remaining morphospace. This is perhaps not unexpected, as one might expect evolutionary and ecological factors to drive variation once the relationship between size and shape is removed. It is also worth noting that semiaquatic species cluster with Microgale mergulus unless evolutionary history is transformed out of the analysis. This should not be taken to imply that M. mergulus is closely related to this clade, but rather that the derived anatomical variation within the Potamogalidae is correlated with the semiaquatic ecomorphotype, and thus shows exceptional inter-family convergence between two widely separated branches of the Tenrecomorpha.
Tenrecidae: Geogalinae
The sole member of the subfamily Geogalinae, Geogale aurita, has previously been placed ancestral to the other tenrec clades (Olson and Goodman 2003), and for this reason has been interpreted as exhibiting markedly conservative brain characters (e.g., Stephan et al. 1991). Despite its diminutive size, G. aurita occupies a distinct region of the unadjusted morphospace along the minimum extreme of PC1 and exhibits on the whole a far more elongate endocast than the comparably sized oryzorictines. These features are clearly distinct from other smaller tenrecs, and indeed, when adjusting for allometry (Fig. 4b) G. aurita occupies an even more isolated region of the morphospace, robust to phylogenetic correction. While comparatively little is known about the hunting ecology of this animal outside of an apparent propensity for termites (Stephenson and Racey 1993), the piriform lobe and expanded olfactory regions suggest a strong reliance on olfaction in this clade. Despite a small overall size and likely similar ecology, this tenrec stands in contrast to the oryzorictines (see below) in having an elongate and clearly differentiated endocast compared to the flattened, rounded one characteristic of the latter clade (particularly members of the genus Microgale).
Tenrecidae: Tenrecinae
The Tenrecinae display remarkable disparity across the endocast morphospace. Interspecies differences are certainly emphasized by allometry, Tenrec being the largest extant tenrecomorph (Nicoll 2003), but persist even when the allometric signal is removed from the dataset. Morphospace spread among tenrecines may be explained in part by habitat, as Hemicentetes is found in mesic habitats, while all others are xeric or eurytopic (see Online Resource 1). It should be noted that the tenrecinces have evolved spines, and it has been previously suggested that this clade evolved both spinescence and reduced brain size in tandem with moving into open habitats (Stankowich and Stensrud 2019). Endocasts of Setifer and Echinops, the hedgehog tenrecs, cluster near each other under nearly all permutations (Fig. 4) a distinct change in shape that corresponds to the overall shrinkage in encephalization quotient (see Stankowich and Stensrud 2019. Hemicentetes semispinosus, by contrast, is positioned within the oryzorictine group in the morphospace. Its broad hindbrain sets H. semispinosus apart from the rest of the tenrecs in this analysis, highlighted in PC3 of the unaltered analysis. This may be related to its unique ecology, specializing on earthworms (Eisenberg and Gould 1969), utilizing a unique stridulating organ (Endo et al. 2010), and perhaps even echolocating (Gould 1965). The proximity of Hemicentetes to the fossorial Oryzorictes in the first principal component of all analyses (Figs. 3 and 4) could be explained in part by convergence in ecomorphotypes, as foraging through sound or vibrissae may lead to a reduction in the presumably ancestrally large olfactory bulbs and flattened cortex seen in all other tenrecines. Although not all fossorial mammals converge on the same brain shape (Stephan et al. 1991), reduced cortices and flattened skulls are consistent with this trend (Bertrand et al. 2018).
In contrast to these relatively extreme examples among tenrecine species, the genus Tenrec occupies a central region of the morphospace, varying from its nearest relative, Hemicentetes, principally along PC1 (corresponding to larger, more defined olfactory bulbs, smaller petrosal lobules, and a flattened cerebellum). While a remarkably generalist taxon with respect to diet and habitat, Tenrec is known to use scent for investigation and communication (Eisenberg and Gould 1969), and the olfactory acuity indicated by its endocast may play a role in facilitating these behaviors.
Tenrecidae: Oryzorictinae
The most speciose clade in this analysis, Oryzorictinae, is marked by broadly similar endocast shape, comparatively anteroposteriorly short endocast (with a rounded neocortex, piriform lobes that dip below the base of the endocast), and comparatively large flocculi and paraflocculi in all species in this subfamily. This general pattern is robust to allometric and phylogenetic corrections, but there are exceptions. Microgale mergulus consistently groups convergently with less closely related but similarly semiaquatic taxa. Nesogale species are distinct from all other tenrecs in the unadjusted analyses, but this may be in large part due to allometry. Once allometry is accounted for, these species group within the Microgale cluster, echoing their traditional classification as members of this genus (MacPhee 1987). Perhaps more intriguing is the lack of ecological distinctiveness among these tenrecs: apart from the convergence of M. mergulus on other semiaquatic taxa, the arboreal, xeric, and fossorial members of Microgale do not form divergent groupings with taxa outside this clade. Some of this may be due to shared allometric effects – indeed, after allometric adjustment, the fossorial Oryzorictes and M. gymnorhyncha pull away from the cluster grouping to a distinct morphospace region. The lack of an exceptional fossorial signal in the endocast is in keeping with findings on fossorial insectivorous boreoeutherians (Stephan et al. 1991), as fossorial mammals may forage through smell, somatosensation, echolocation, or any combination of the three. In the case of these taxa, it may be foraging through smell that is paramount, given the extended olfactory regions of the brain. For the prehensile-tailed arboreal tenrecid M. longicaudata, the lack of an exceptional endocast shape is something of a puzzle – perhaps the signifiers of ecological signal are better expressed in secondary sensory structures such as the semicircular canals, or the diminutive size of the animal allows for a less-extreme mode of arboreality than a prehensile tail might imply.
Ancestral endocast shape for tenrecs
The ancestral node for the common ancestor of the Tenrecidae is reflected in the mean of the unadjusted dataset (Fig. 3), which stands not only outside the Microgale cluster but also quite apart from any clearly defined ecomorphological grouping. The ancestral node for the potamogalids, on the other hand, lies within the morphospace region occupied by semiaquatic taxa with a corresponding reduction in olfactory size and paleocortex, indicating the strong role this ecomorphotype may have played in the history of extant tenrecomorphs on the African continent. Similarly, the history of the Oryzorictinae appears to show allometric shrinkage with each node approaching Microgale, while the ancestral node for the tenrecines represents a midpoint between extremes of this clade’s shape disparity and not, as some have claimed, proximity to Geogale.
In the absence of relevant fossil data, these findings remain tentative and imprecise, as they are based only on the specimens available for this study. Apart from a few late Quaternary taxa (e.g., MacPhee 1986) that differ little from their close relatives, the nature of the fossil record for Malagasy tenrecomorphs was largely ignored until Buckley (2013) proposed on paleoproteomic grounds that the enigmatic genus Plesiorycteropus might be a member of this group. Osteological morphology provides little support for this hypothesis or its traditional alternative that, among extant afrotheres, Plesiorycteropus is most closely related to Orycteropus (for various evaluations of the evidence see MacPhee 1994; Sanchez-Villagra et al. 2007; Tabuce et al. 2008). Although this taxon was not included in the present analysis, limited endocast evidence (Thewissen 1985) indicates that, as compared to Orycteropus, Plesiorycteropus exhibits a vermis higher than the neocortex and shortened olfactory bulbs. These features are consistent with the projected morphospace region occupied by tenrecines in this study and, interestingly, bear some anatomical similarities to the extremes of PC3 seen in H. semispinosus. Speculatively, the high vermis and shortened olfactory bulbs could be interpreted as traits ancestrally common to tenrecines prior to the evolution of spinescence and related xeric adaptations, but this obviously needs closer study. Alternatively, ontogenetic studies on the cranial endocast of the capybara (Ferreira et al. 2022) suggest that that a comparative lengthening of the olfactory bulbs and a flattening of the vermis occur during ontogenetic growth. It may thus be possible that the divergence observed in H, semispinosus could be the result of paedomorphism within this tenrecine branch. In any case, further testing of the possible inclusion of Plesiorycteropus within tenrecs is highly warranted.
Comparisons to other mammalian clades
It should be noted that, despite their considerable diversity, the vast majority of tenrecomorph disparity is located on the island of Madagascar. The radiation of Malagasy tenrecs possibly took place in a competitive vacuum; there are no other similarly insectivorous mammals on the island apart from two species of the Neogene soricid Suncus (one introduced) (Stephenson et al. 2003). By contrast, apart from a few highly incomplete Miocene fossils (Butler and Hopwood 1957; Asher et al. 2006), the semiaquatic Potamogalidae are the only members of the clade on the African mainland, while multiple species of terrestrial insectivores of different mammalian clades (especially the soricid Crocidura) are present in the form of a number of ecotypes (Stephenson et al. 2003). In the absence of a substantial Paleogene fossil record for mainland Africa, the actual factors behind the lack of extant African terrestrial tenrecs, and the role competition (or lack thereof) may have played in the divergent evolutionary paths of each lineage, can only be surmised.
In addition to intra-clade disparity, a growing body of mammalian endocast research permits comparison to other clades of mammals investigated with similar techniques. Strong phylogenetic groupings and a slight degree of fossorial homoplasy can be detected in squirrels and their allies (Bertrand et al. 2019), a situation reminiscent of the tenrecs (particularly within the Oryzorictinae, excluding M. mergulus). Studies of the cranial endocasts of marsupial taxa also recover a “elongate” to “rounded” continuum within endocast shape (Weisbecker et al. 2021), another correspondence to conditions amongst the Tenrecomorpha. In contrast, it should be noted that these studies showed little in the way of significant allometric effects. The distinct convergence of phylogenetically distinct semiaquatic taxa and the statistically significant effects of allometry appear, for now, to set this clade of afrosoricids apart.
This is the first study investigating the tenrecomorph sensory apparatus via the use of virtual endocasts derived from high-resolution uCT imaging. Our results provide strong evidence for the influences of phylogeny, allometry, and ecology (as exemplified through ecomorphology and habitat) in the evolution of tenrecomorph taxa. The size and shape of tenrecomorph brains are clearly related, with larger taxa having elongate endocasts and particularly large olfactory bulbs, and smaller taxa having a more “rounded” shape. The clustering of the Oryzorictinae and Potamogalidae suggests the presence of plesiomorphic shapes within each group, which may be related to size in the oryzorictines – removing allometry from the analysis reveals additional variation (particularly with respect to semifossorial taxa) – and to semiaquatic ecology in the case of potamogalids. By contrast, the Tenrecinae and the sole species of Geogalinae are uniquely disparate, occupying widely different regions of the morphospace even under multiple rounds of adjustment for allometry and phylogeny, and lacking ecomorphological clustering with other groups. The distinct strength and significance of allometric and evolutionary signals present in the subfamily groups within Tenrecidae should be kept in mind when analyzing and interpreting the sensory morphology of these clades. Regardless of the root cause of disparity across the species in the clade, this study highlights the exceptional variety of these enigmatic insectivores and presents a foundation for further study of the tenrecomorph sensory system.
Data availability
The data that support the findings of this study are available from the corresponding author, R.B.S., reasonable request.
Code availability
All functions listed were run in R ver.4.0.5, with packages ggPlot and Geomorph. Code is available from the corresponding author upon request.
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Acknowledgements
Collections made available from the American Museum of Natural History (AMNH) and The Field Museum (FMNH). We would like to thank John Flynn for guidance and assistance throughout, Katie Everson for sharing phylogenetic data, Morgan Hill and Andrew Smith for data management and CT operation assistance, and Neil Duncan, Ellie Hoeger, Marisa Surovy, Sara Ketelson, Bruce Patterson, and Stephanie Smith for access to collections and specimens. Funding in part by National Science Foundation Graduate Research Fellowship Program (NSF GRFP) No. 1447167 and Richard Gilder Graduate School (RGGS) Graduate Fellowship
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Conceptualization: all authors. CT visualization and morphometric analyses: R.B.S. Providing access to museum collections and equipment: R.D.E.M. Interpretation of anatomical data: all authors. Graphics: R.B.S. Writing: all authors, and approved by all authors.
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Online Resource 3: Morphospace results from the first two components of principal component analyses (PCA) on all endocasts used in this study performed under additional transformations (PDF 316 KB)
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Benjamin Sulser, R., MacPhee, R.D.E. An island apart: Cranial endocast variation and sensory function in Tenrecomorpha. J Mammal Evol 30, 615–626 (2023). https://doi.org/10.1007/s10914-023-09662-8
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DOI: https://doi.org/10.1007/s10914-023-09662-8