Abstract
There is increasing concern over the ecological impact of markedly increasing numbers of large herbivores (hereafter large herbivore overabundance) on forest ecosystems. To predict the ecological consequences of large herbivore overabundance, it is first necessary to understand how biological communities respond to large herbivore overabundance. Here, we examined the relationships between the life history traits of five insect taxonomic groups (moths, dung beetles, longicorn beetles, carabid beetles, and carrion beetles) and their responses to deer overabundance in Hokkaido, northern Japan. Insects were collected from three study sites: enclosure (20 deer/km2), control (10 deer/km2), and exclosure (0 deer/km2). The different taxonomic and functional insect species differed in their response to deer overabundance. The abundance (number of individuals) of longicorn beetles, dung beetles, and arbor-feeding moths was higher in the enclosure site than in the control site, whereas that of carabid beetles, carrion beetles, and herb- or shrub-feeding moths was higher in the control site than in the enclosure site. These results suggest that the type of food and the level of dependence on the understory are key traits determining insect sensitivity to deer overabundance. In addition, large or flightless species responded negatively to deer overabundance. Overall, this study demonstrated a significant change in insect communities following experimental deer overabundance, suggesting that large herbivore overabundance leads to the homogenization of biological communities. Unfortunately, because insects have diverse functions in forest ecosystems, such marked changes in both abundance and composition of insect communities will decrease ecosystem functions and resilience.
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Introduction
There is increasing concern over the ecological impact of markedly increasing numbers of large herbivores (large herbivore overabundance) on forest ecosystems (Fuller and Gill 2001; Côté et al. 2004; Martin et al. 2010). Since the late twentieth century, large mammalian herbivores have become overabundant in the northern hemisphere, including North America, Europe, and East Asia, which is largely due to the development of agriculture and forestry, predator loss, and reduced hunting (Rooney 2001; Côté et al. 2004; Uno et al. 2009). Large herbivore overabundance affects understory and tree regeneration through browsing and bark stripping (Akashi and Nakashizuka 1999; Vera et al. 2006; Rooney 2009). Indeed, many studies have reported that over-browsing by large herbivores alters the composition and abundance of understory species (Rooney 2009; Suzuki et al. 2013). Bark stripping by large herbivores also damages small trees, which in turn enhances tree mortality rates (Akashi and Nakashizuka 1999; Vospernik 2006). Furthermore, animal species that depend on forest environments are also indirectly influenced by the overabundance of large herbivores (invertebrates: Allombert et al. 2005; Minoshima et al. 2013; Teichman et al. 2013, birds: Chollet and Martin 2013; Teichman et al. 2013).
To manage and conserve forest ecosystems and ultimately reverse the ecological consequence of large herbivore overabundance, it is first necessary to understand and predict how biological communities respond to large herbivore overabundance (Côté et al. 2004). Previous research shows that different species and taxa respond differently to large herbivore overabundance (Melis et al. 2006; Suzuki et al. 2013; Bachand et al. 2014), which makes general predictions difficult. A life history trait-based approach that explores the relationships between life history traits and the sensitivity of species to environmental change enables us to gain insights into this issue. This approach is appropriate because life history traits are usually linked directly with various types of environmental change such as habitat loss and fragmentation and climate change (Jiguet et al. 2007; Ockinger et al. 2010; Soga and Koike 2012). Identifying the key traits that control species responses to large herbivore overabundance allows us not only to predict future species loss and change in community compositions following large herbivore overabundance (McKinney and Lockwood 1999) but also to predict changes in overall ecosystem function and resilience (Petchey and Gaston 2006; Mori et al. 2013). However, this approach has rarely been used in this field. To date, empirical studies that have investigated relationships between life history traits and the response of species to large herbivore overabundance are quite scarce and limited (Filazzola et al. 2014; Koike et al. 2014).
To investigate the relationships between species life history traits and their sensitivities, insects are excellent model organisms. This is because many insect species have species-specific life history traits, and they respond quickly to environmental change (Sumways 2007; Maleque et al. 2009). For insect species, types of food resources and the degree of dependence on plant species are expected to directly determine the sensitivity to large herbivore overabundance. Indeed, it is known that while the abundance of dung beetles increases with an increase in mammalian abundance (Stewart 2001; Nichols et al. 2009), herbivorous insects respond negatively (Den Herder et al. 2004; Teichman et al. 2013). Yet, among herbivorous insects, those that utilize tall trees as a food resource are less likely to respond negatively because the leaves of tall trees are located above the browsing height, yet sometimes they respond positively because of compensating growth due to browsing by the large herbivores (Gill 1992; Guillet and Bergström 2006). Furthermore, Allombert et al. (2005) and Mysterud et al. (2010) observed that while herbivorous insects, which typically inhabit understory vegetation, drastically responded to large herbivore overabundance, litter-dwelling arthropods showed a weaker response. Other researchers have observed a significant association between insect body size and sensitivity to large herbivore overabundance (Cole et al. 2006; Koike et al. 2014). It has often been reported that large species are vulnerable to various types of environmental change because they cannot adapt quickly to a disturbed environment due to their longer generation times (Ribera et al. 2001; Jelaska and Durbešic 2009). Besides body size, flight ability is also a key ecological trait for determining response to environmental change because flight ability directly links to dispersal ability (Den Boer 1990). Because flightless (apterous) species have lower dispersal ability, they are less likely to move quickly to suitable habitats (Den Boer 1990).
Here, we evaluate the effects of sika deer (Cervus nippon) overabundance on insect communities in a broad-leaved forest in Hokkaido, northern Japan. In Japan, deer populations have continuously increased since the late twentieth century (Uno et al. 2009), and the dramatic increase has degraded forest ecosystems across Japan, including soil erosion (Sakai et al. 2011) and declining vegetation (Takatsuki 2009). In this study, we investigated the abundance (number of individuals) of insects belonging to five different taxonomic groups (moths, dung beetles, longicorn beetles, carabid beetles, and carrion beetles). The aim of this study was to clarify the relationships between life history traits of insects and their responses to large herbivore overabundance. We predicted the following outcomes: (1) insect groups whose food resources increase with increasing deer density (dung and longicorn beetles) respond positively to deer overabundance, (2) groups that strongly depend on understory vegetation (herb or shrub feeders such as moths and ground beetles) respond negatively, and (3) large or flightless species respond negatively to deer overabundance.
Materials and methods
Study area and sampling sites
Our study area is located in the Tomakomai Experimental Forest (TOEF) of Hokkaido University (TOEF; 42°40′N, 141°36′E) in western Hokkaido, northern Japan. The mean annual temperature is 6.5 °C and the mean monthly temperature ranges from 19.1 to −3.2 °C. The annual precipitation is about 1200 mm, and the mean snow depth ranges from 20 to 50 cm. In TOEF, there are 2 fenced sites: a 16.4 ha enclosure and a 1.5 ha exclosure. At the enclosure and exclosure sites, the deer density has been manipulated since 2004 (Fig. 1). At the enclosure site, the deer density has been maintained at a high level (20 deer/km2). At the exclosure site, the deer have been excluded completely (0 deer/km2). The study was carried out in the 2 manipulated sites and in the surrounding area (hereafter called “control sites”). At the control site, deer densities estimated by light censuses were approximately 10 deer/km2 (TOEF, Unpublished results). The study area is situated in a secondary deciduous forest dominated by Japanese oak (Quercus crispula), painted maple (Acer mono), and Japanese linden (Tilia japonica). The understory vegetation is dominated by wood fern (Dryopteris crassirhizoma), Maianthemum dilatatum, and Japanese spurge (Pachysandra terminalis) (Hiura 2001).
Insect sampling
We surveyed insects from May to September 2014. Surveys were conducted under appropriate weather conditions (without rain). Accidentally, if there was rainy day during the sampling period, the sampling period was extended for 1 day. For each site, we set 6 sampling plots (three plots in two study periods; Fig. 1). These 6 plots were placed at least 50 m apart from each other and deer fences, following previous studies (Larsen 2005; Van Grunsven et al. 2014), at enclosure and control sites. Due to limited space at the exclosure site, sampling plots were placed as far apart as possible (ca 15 m from each other and ca 30 m from deer fences). We used 5 % acetic acid as a preservative solution for all types of traps (except the light trap), and the captured individuals were then dried, mounted, and identified to the level of species in the laboratory.
We sampled longicorn beetles using collision traps baited with benzyl acetate and ethanol. These traps consist of 2 crossed collision plates (approximately 210 mm high and 220 mm wide), a roof, and a bucket partially filled with preservative solution. These traps were set at 1.5 m from the ground. The species collected by the collision traps mainly consisted of flower-visiting species (Sayama et al. 2005). Therefore, to sample non-flower-visiting species, in addition to the collision trap, longicorn beetles were sampled by using malaise traps. Collision traps were set for 3 days in mid-June and mid-July, and malaise traps were set for 4 days in early June and from late July to early August. During these periods, the abundance of longicorn beetles is thought to be highest and flowers abundant (Hasegawa et al. 2007; Inari et al. 2012). To prevent interference between the two types of traps, we avoided overlapping their sampling periods.
We sampled moths using light traps (Okochi 2002). The light traps were constructed from two boxes made with nylon net illuminated by 4-W fluorescent light and 4-W UV light. One of the boxes was positioned over the lights and another box was positioned under the lights. The bottom of the box positioned over the lights was open, and the top of the box situated under the lights had a funnel shape to prevent the moths from escaping. Each light trap and light source were located 1.5 m from the ground and were operated for 1 night during 3 different periods (mid-June, mid-July, and mid-September) to avoid the effect of seasonal variation on the species composition (Yoshida 1980).
We sampled dung and ground beetles using pitfall traps baited with cattle dung and fermented milk drink. Pitfall traps were made from plastic containers (22.5 cm diameter and 26.6 cm deep) and plastic cups (8.3 cm diameter and 11.5 cm deep). The container of preservative solution and fermented milk drink was buried to its rim in the ground. Then, plastic cups with cattle dung were hung inside the container using wires. To prevent trap destruction by mammals or carnivores, the pitfall traps were covered with an iron fence, and chili powder was scattered around the trap. We also set plastic roofs on the traps to prevent interference from rain and fallen leaves. Pitfall traps were set for 3 days in mid-May, mid-June, and mid-September to cover the peaks of activity of these beetles (Sota 1987; Yusa and Nishiguchi 1989; Kanda et al. 2005).
Environmental conditions
We measured the understory layer (<1.5 m from the ground) cover and diversity, shrub layer (≥1.5 m, <3.0 m) and canopy layer (≥3.0 m) cover, tree species diversity, leaf litter depth, canopy openness, and dead wood volume (fallen trees and standing dead trees). These surveys were conducted in quadrats (20 × 20 m2) established at each site (4 quadrats each in the enclosure and control sites, 2 quadrats in the exclosure site). Leaf litter depth and the dead wood volume were measured in May, and the other parameters were measured in May, June, July, and September.
The understory layer cover was measured in 2 subquadrats (1 × 1 m2) established in each quadrat. To measure the α diversity of the understory vegetation, we calculated the Shannon–Wiener index based on the importance value (IV) score instead of simple abundance (Zou et al. 2013). The IV score of each species was calculated from the relative cover rank (1: <10 %; 2: ≥10 and <25 %; 3: ≥25 and <50 %; 4: ≥50 and <75 %; 5: ≥75 %) and height of each species in the subquadrats. To measure the α diversity of the tree species, the Shannon–Wiener index was also calculated based on the IV score using data from Hino et al. (Unpublished results). The IV score for each tree species was calculated from relative abundance and breast height area. The IV score for the ith species (IV i ) was calculated as:
where d is the cover rank of the ith species for the understory vegetation, but for the tree species, d is the number of individuals of the ith species; h is the height of ith species for understory vegetation, but for the tree species, d is the breast height area of the ith species; and S is the total number of species in a sample plot. Leaf litter depth, canopy openness, and dead wood volume were measured at the quadrat scale. The leaf litter depth was measured using a ruler inserted vertically and 5 times randomly in each quadrat. A hemispherical photograph was taken at the center of each quadrat using a digital camera equipped with a fish-eye lens. Canopy openness was calculated by CanopOn-2 software (Takenaka 2009). Fallen trees greater than 5-cm diameter at the point where it intersected a line were investigated in each quadrat (Ugawa et al. 2012). The length (l) and diameter (a i ) at 2-m intervals were measured for each fallen tree. Each fallen tree volume (V) was calculated using Huber’s formula:
where n is the distance from the base of each of fallen tree (n = 1, 3, …, n m from the base) and j is (n + 1) m from the base. To measure dead wood volume, we measured the diameter at breast height (DBH) and the height of whole dead stands greater than 5-cm DBH in each quadrat. Each dead wood volume was calculated using the formula for a circular cone (Hiura et al. 1998).
Species classifications
For each insect group except the longicorn beetles, the insect species observed in the field were divided into different functional groups by morphology or life history traits (Online Resource Tables 3–6). Moths were divided into herb- or shrub-feeding species or arbor-feeding species by their larval food habit. Based on this classification, 63 species (5450 individuals) that feed on both types of plants, 104 species (2107 individuals) that feed on other types of plants (i.e., litter), and 59 species (3060 individuals) with unknown larval food habits were not divided into any groups. Dung beetles were divided according to body size (S: <10 mm; L: ≥10 mm). Ground beetles and wingless (apterous or macropterous) insects were divided according to body size (S: <10 mm; M: ≥10 and <20 mm; and L: ≥20 mm). Species whose wing form varied significantly from individual to individual [carabid beetles: three species (2086 individuals), carrion beetles: one species (35 individuals)] were divided into neither apterous nor macropterous groups. In the latter analysis, however, small-sized carabid beetles were excluded because of the small sample size (total number of individuals <10). Longicorn beetles were not divided into any functional groups due to data limitation: 6 species (34 individuals) collected from the collision traps and 13 species (48 individuals) collected from the malaise traps.
Data analysis
To test for differences in environmental conditions between the three sites (enclosure, exclosure, and control sites), we performed a Kruskal–Wallis test, using the “kruskal.test” function followed by multiple pairwise comparison of the sites using the Wilcoxon rank sum test, using the “pairwise.wilcox.test” function (R version 2.15.2; R Core Team 2012). Our preliminary analysis demonstrated no evidence of spatial autocorrelation in the data across the 18 sampling sites. Therefore, we treated the data from each site as spatially independent samples in later analyses.
To test whether insect communities differed between the three sampling sites (enclosure, exclosure, and control sites), we used generalized linear models (GLMs), using the “glm” function (R version 2.15.2; R Core Team 2012). For GLMs, the abundance of each taxonomic or functional group was used as a response variable with a Poisson distribution and a log link function. Sampling sites were used as categorical explanatory variables (we used control sites as a reference). For carrion beetles, however, macropterous species were not captured at the enclosure site. Thus, the comparison of abundance of macropterous species was only conducted between the control and exclosure sites. In this study, the number of sampling days differed between the plots due to trap destruction by wind or animals. Thus, we used sampling period (days) as the offset term. We calculated estimated abundance using the estimates of parameters in GLMs. All statistical analyses were performed using R ver.2.15.2 (R Core Team 2012). In all of the analyses, we defined the p value as 0.05.
Results
Environmental conditions
Results from the Kruskal–Wallis test revealed that the cover of the understory layer (p = 0.003) and leaf litter depth (p < 0.001) were significantly different between the 3 study sites (Online Resource Fig. 1a and h; Table 1). The cover of the shrub layer (p = 0.08) and dead wood volume (p = 0.06) were also marginally different between the 3 sites (Online Resource Fig. 1b and f; Table 1). The Shannon diversity of the understory (p = 0.60), tree species (p = 0.42), the cover of the canopy layer (p = 0.22), canopy openness (p = 0.73), and fallen tree volume (p = 0.16) did not significantly differ between the 3 sites (Online Resource Fig. 1; Table 1). Pairwise Wilcoxon rank sum tests showed a significant difference in understory cover between the exclosure and enclosure sites (p = 0.03), between the control and enclosure sites (p = 0.04), and a difference in leaf litter depth between the exclosure and enclosure sites (p = 0.002; Online Resource Fig. 1).
Insect communities
In total, 18,589 individuals belonging to 384 species were sampled. For longicorn beetles, 6 species (34 individuals) were collected by collision traps and 13 species (48 individuals) were collected by malaise traps (Online Resource Table 3). For moths, 339 species (14,084 individuals) were observed (Online Resource Table 4). These species included 122 arbor feeders (2621 individuals) and 54 herb or shrub feeders (856 individuals). For dung beetles, 3 small species (292 individuals) and 1 large species (1024 individuals) were sampled (Online Resource Table 5). For carabid beetles, 1 small (8 individuals), 8 medium-sized species (2880 individuals), and 7 large species (122 individuals) were captured (Online Resource Table 6). Carabid beetles were also grouped to 8 apterous species (588 individuals) and 5 macropterous species (336 individuals). For carrion beetles, 4 medium-sized (62 individuals) and 1 large (35 individuals), and 1 apterous species (52 individuals) and 3 macropterous species (10 individuals) were collected (Online Resource Table 7).
For longicorn beetles collected by malaise traps, abundance in the enclosure site was significantly higher than in the control site (p = 0.001; GLM; Fig. 2a; Online Resource Tables 1–3). For longicorn beetles collected by collision traps, significant differences in abundance between the 3 sites were not observed (GLM; Fig. 2b; Online Resource Tables 1–3).
For moths, the total abundance was lower in the enclosure than in the control site (p < 0.001; GLM; Fig. 2c; Online Resource Tables 1, 2 and 4). Abundance of arbor feeders was lower in the exclosure than in the control site (p = 0.03), whereas abundance of herb or shrub feeders in the exclosure was significantly lower than in the control site (p = 0.02; GLM; Fig. 3a; Online Resource Table 1, 2 and 4).
For dung beetles, the total abundance in the control site was significantly lower than in the enclosure (p = 0.002) and exclosure sites (p = 0.002; GLM; Fig. 2c; Online Resource Tables 1, 2 and 5). Abundance of large dung beetles in the control site was also significantly lower than in the enclosure (p < 0.001) and exclosure sites (p < 0.001; GLM; Fig. 3b; Online Resource Tables 1, 2 and 5). For small dung beetles, abundance did not differ between the 3 sites (GLM; Fig. 3b; Online Resource Tables 1, 2 and 5).
For carabid beetles, the total abundance in the control site was significantly higher than in the enclosure (p < 0.001) and exclosure sites (p < 0.001; GLM; Fig. 2d; Online Resource Tables 1, 2 and 6). Abundance of medium-sized carabid beetles in the control site was significantly higher than in the enclosure (p < 0.001) and exclosure sites (p < 0.001), whereas the abundance of large beetles did not differ between the 3 sites (GLM; Fig. 3c; Online Resource Tables 1, 6). Abundance of apterous species in the enclosure site was significantly lower than in the control site (p = 0.001; GLM; Fig. 3c; Online Resource Tables 1, 2 and 6). Abundance of macropterous species was significantly higher in the exclosure than in the control sites (p = 0.02; GLM; Fig. 3c; Online Resource Tables 1, 2 and 6).
For carrion beetles, the abundance in the control site was significantly higher than in the enclosure (p < 0.001) and exclosure sites (p = 0.03; GLM; Fig. 2e; Online Resource Tables 1, 2 and 7). Abundance of medium-sized species was significantly lower in the enclosure than in the control sites (p < 0.001; GLM; Fig. 3d; Online Resource Tables 1, 2 and 7). Abundance of large species did not differ between the 3 sites (GLM; Fig. 3d; Online Resource Tables 1, 2 and 7). Abundance of apterous species in the enclosure site was significantly lower than in the control site (p = 0.003), whereas abundance of macropterous species did not differ between the 3 sites (GLM; Fig. 3d; Online Resource Tables 1, 2 and 7).
Discussion
To predict the ecological consequences of large herbivore overabundance, it is first necessary to understand how biological communities respond to large herbivore overabundance. This study is an attempt to investigate the response of various taxonomic groups of insect species to deer overabundance. We found that the sensitivity of response to large herbivore overabundance differed greatly between the different taxonomic or functional groups.
In our study, carabid beetles, carrion beetles, and herb- or shrub-feeding moths responded negatively to deer overabundance (Figs. 2, 3; Online Resource Table 1). As carabid and carrion beetles use the understory as their habitat, a declining understory layer would have a negative effect on these species (Latty et al. 2006; Taboada et al. 2008). Indeed, we observed that the understory layer cover declined with increasing deer density (Online Resource Fig. 1a; Table 1), supporting the above explanation. Because herb- or shrub-feeding moths typically use understory plants as food resources, these species are likely to compete with deer for food resources (Teichman et al. 2013; Schweitzer et al. 2014). In contrast, arbor-feeding moths did not respond negatively to deer overabundance (Fig. 3). Arbor feeders do not use the understory vegetation as a food resource; therefore, they would be less dependent on the understory than the herb or shrub feeders. Therefore, our results suggest that dependence on the understory is one of the key factors determining the response of insects to deer overabundance (Allombert et al. 2005; Mysterud et al. 2010). Because plants are fundamental components of survival and reproduction, the trends seen in our results are likely to apply to other taxonomic species including birds and small mammals (Flowerdew and Ellwood 2001; Chollet and Martin 2013).
In contrast to the above-mentioned insect groups, arbor-feeding moths, longicorn beetles, and dung beetles responded positively to deer overabundance (Figs. 2, 3; Online Resource Table 1). Surprisingly, we observed that the number of arbor-feeding moths was higher in the enclosure than in the control site (Fig. 3). Although deciphering the mechanism behind this pattern is difficult, it is possible that the quality of food for the arbor-feeders increased with increasing deer density. Lucas et al. (2013) indicates that deer overabundance enhances the growth rate of mature trees because deer defecation accelerates nutrient cycling and deer browsing weakens competition between trees and understory plants. Longicorn beetles also responded positively to deer increases (Fig. 2). As the majority of species observed in this study use dead wood as a larval food resource, the increase in dead wood volume in the enclosure site (Online Resource Fig. 1f; Table 1) is likely to enhance their food resource. Indeed, it has been reported that bark stripping by deer increases the dead wood volume (Akashi and Nakashizuka 1999; Côté et al. 2004). In this study, however, longicorn beetles collected in the collision traps were not abundant in the enclosure site (Fig. 2). A reason for this could be that the longicorn beetles captured by collision traps consisted mainly of flower-visiting species (Online Resource Table 3). For dung beetles, it is well known that abundance of dung beetles and mammals is closely linked to each other because dung beetles depend on mammal dung as an adult and larval food resource (Andresen and Laurance 2007; Nichols et al. 2009). Although we did not measure the amount of deer dung, the increased abundance of dung beetles in the enclosure site is likely due to increasing deer dung (Fig. 2). Therefore, our results suggest that the type of food is also a key factor determining the response of insects to deer overabundance.
We observed that body size and wing form were also associated with the response of insect species to deer overabundance. In this study, apterous species tended to respond negatively to deer overabundance (Fig. 3). This result concurs with previous studies that demonstrate that flightless species are more susceptible to disturbance (Jelaska and Durbešic 2009; Pakeman and Stockan 2014). In addition, smaller species responded negatively to deer overabundance (Fig. 3). For dung beetles, it has been reported that smaller species are more vulnerable to understory decline by deer over browsing because smaller species favor more structurally complex habitats (Koike et al. 2014). For carabid beetles, our results contrasted with previous studies, which found that large species are more vulnerable to disturbance (Rainio and Niemelä 2003; Brose 2003; Cole et al. 2006). One reason for this is that, unlike these previous studies, the majority of the smaller species observed in the field were flightless species (e.g., Pterostichus thunbergii and Silpha perforata venatoria).
Although the results were clear, this study inevitably had some limitations. First, our data were collected at Hokkaido from only three study sites, and the scale was relatively small. Therefore, caution is warranted for any generalizations of the findings. Second, pitfall and light traps were the most common methods used for measuring abundance and species richness of insect species; however, such passive trapping methods can be affected by several factors (e.g., habitat condition and trap size) (Koivula et al. 2003). Thus, more detailed assessments may be needed. Third, this study employed a cross-sectional design (comparing three study sites comprising different deer densities); therefore, we could not directly assess the effect of a deer population increase on insect communities. Further longitudinal studies could clarify this issue. Finally, a function describing the threshold at which insect communities sharply respond to a deer population increase remains to be determined, thereby posing a challenge for future studies.
In conclusion, our study demonstrated a significant change in insect communities following experimental deer overabundance in Japan. Some taxonomic or functional groups whose food increases with deer overabundance responded positively to deer overabundance (arbor-feeding moths, longicorn beetles, and dung beetles), although others who depend on understory vegetation responded negatively (herb- or shrub-feeding moths, carabid beetles, and carrion beetles). These results suggest that large herbivore overabundance in forest ecosystems leads to the homogenization of biological communities (McKinney and Lockwood 1999). Our study also suggests that the type of food and the level of dependence on the understory are key factors determining the response to large herbivore overabundance. Our results also imply that as the deer density increases, the biological communities will become largely dominated by small or highly mobile generalist species. Our results would help not only to predict the effect of large herbivore overabundance but also to understand the appropriate density of large herbivores to conserve forest ecosystems. Further research should attempt to detect the thresholds of these changes (Côté et al. 2004). Importantly, insects perform a wide variety of functions in forest ecosystems, such as the decomposition of dung and carcass (Nichols et al. 2008; Sugiura et al. 2013), pollination (Matsuki et al. 2008), and nutrient cycling (Cigan et al. 2015). Therefore, unfortunately, drastic changes in both abundance and composition of insect communities will also decrease ecosystem function and resilience (Hooper et al. 2005).
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Acknowledgments
The field survey was supported by Tomakomai Experimental Forest of Hokkaido University. This study was partly funded by JSPS no. 25292085, by the Ministry of Environment (SG-3), by 26th Pro-Natura Fund in Japan, and by Hokkaido University no. 2015_04_D.
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Iida, T., Soga, M., Hiura, T. et al. Life history traits predict insect species responses to large herbivore overabundance: a multitaxonomic approach. J Insect Conserv 20, 295–304 (2016). https://doi.org/10.1007/s10841-016-9866-x
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DOI: https://doi.org/10.1007/s10841-016-9866-x