Abstract
Myocardial cell injury or cardiomyopathy is associated with excessive inflammatory response and apoptosis of cardiac myocytes during sepsis. MicroRNA-23b (miR-23b) is a multifunctional miRNA that is considered to regulate immunosuppression in sepsis. The aim of this study was to examine the effect of miR-23b on cardiomyopathy induced by sepsis and to explore the potential mechanism involved. Sprague-Dawley rats were subjected to cecal ligation and puncture (CLP), and the level of miR-23b at different time points was measured by quantitative real-time polymerase chain reaction (qPCR). Then, we overexpressed miR-23b in vivo and in vitro. The rats were subjected to CLP 7 days after transfection. Cardiac function, inflammatory response, and heart tissues were examined 3 days thereafter. In an in vitro experiment, H9C2 cardiomyoblasts were stimulated with lipopolysaccharide (LPS) after transfection of miR-23b, following which apoptosis and the level of NF-κB were analyzed. The expression of miR-23b was upregulated during polymicrobial sepsis, and transfection of miR-23b lentivirus improved the outcome of sepsis-induced cardiomyopathy by attenuating inflammatory responses and protecting against histopathological damage. In in vitro experiments, elevated miR-23b inhibited excessive apoptosis of cardiomyocytes, which may be because activation of the NF-κB signaling pathway was inhibited by the decreased levels of TRAF6 and IKKβ. Therefore, miR-23b improved sepsis-induced cardiomyopathy by attenuating the inflammatory response, suppressing apoptosis, and preventing NF-κB activation via targeted inhibition of TRAF6 and IκκB. These results indicated that miR-23b may represent a novel therapeutic approach for clinical treatment of sepsis-induced cardiomyopathy.
Similar content being viewed by others
Avoid common mistakes on your manuscript.
INTRODUCTION
Sepsis is a life-threatening organ dysfunction syndrome caused by a dysregulated host response to infection [1], which subsequently results in systemic inflammatory response syndrome and progressive multiple organ dysfunctions, especially cardiac dysfunction [2]. Cardiovascular dysfunction or cardiomyopathy caused by sepsis has been revealed as the major cause of increased mortality in patients with septic shock [3, 4]. Studies have shown that the incidence and prevalence of cardiomyopathy induced by sepsis are as high as 50%, with the mortality reaching 70% [5]; survivors of sepsis also have a high risk of cardiovascular events [6], which are considered manifestations of severe sepsis or septic shock [7]. Hence, the specific pathogenesis and mechanisms of cardiomyopathy in sepsis warrant elucidation.
Sepsis-induced cardiomyopathy (SIC) is characterized by the reversible dysfunction of both the left and right sides of the heart throughout the cardiac cycle (systolic and diastolic) during sepsis [8], which involves a complicated response to pathogens including inflammatory response, mitochondrial dysfunction, altered metabolism, impaired energetics, and structural alterations [9, 10]. Although the myocardial suppression in sepsis has been investigated over past decades [11], the exact pathophysiological mechanisms leading to cardiac dysfunction, as well as effective therapeutic strategies for SIC, require further exploration. Innate immune and inflammatory responses activated by Toll-like receptors (TLRs), which mediate signaling and predominantly activate nuclear factor kappaB (NF-κB), play an essential role in regulating the inflammatory responses in infectious diseases [12, 13]. Moreover, TLR-mediated NF-κB signaling has been considered an important participant in SIC in recent years [14,15,16]. Therefore, targeting the regulation of the TLR-mediated NF-κB signaling pathway could be an effective treatment for SIC.
MicroRNAs (miRNAs) are a class of small non-coding RNAs that have been identified as novel regulators of gene expression at the posttranscriptional level. miRNAs exert strong regulatory effects on eukaryotic genes by contributing to cell proliferation, differentiation, development, metabolism, apoptosis, and other physiological activities [17, 18]. Recent studies have reported that miRNAs are also prognostic predictors of patients with sepsis [19, 20] and almost all cardiovascular processes during sepsis [21,22,23]. MicroRNA-23b (miR-23b) is considered a multifunctional miRNA that can prevent autoimmune diseases via regulation of inflammatory signaling pathways [24, 25] and induction of tolerogenic activity in dendritic cell responses by inhibition of NF-κB signaling [26, 27]. Furthermore, Wu et al. found that miR-23b attenuates progression of sepsis by preventing the expression of inflammatory factors [28]. Considering that SIC is mostly associated with uncontrolled immune and inflammatory response, we speculate that miR-23b represents a therapeutic option for SIC.
In the present study, models of SIC were established using cecal ligation and puncture (CLP) or injection of lipopolysaccharide (LPS) and transfected with miR-23b to observe the effect of miR-23b on the inflammatory responses during SIC and subsequent outcome. We also investigated the effect of regulation of miR-23b and explored the underlying mechanism involved.
MATERIALS AND METHODS
Experimental Animals and CLP Polymicrobial Sepsis Model
Experiments were conducted on 8-week-old male Sprague-Dawley rats weighing 220–260 g (Vital River Laboratory Animal Technology Co., Ltd., Beijing, China). All rats were maintained in separated cages and provided pelleted rat chow with water ad libitum. Rats were housed in a temperature-controlled room with a 12-h light/dark cycle and allowed to acclimatize for 7 days before being sacrificed. All experimental manipulations were undertaken in accordance with the Guide for the Care and Use of Medical Laboratory Animals (Ministry of Health, P.R. China, 1998), with the approval of the Scientific Investigation Board, Tianjin Medical University General Hospital, Tianjin, China. CLP was performed to induce sepsis in rats as previously described [29]. Briefly, rats were randomly divided into four groups: (1) miR-Con, (2) sham, (3) CLP, and (4) CLP + miR-23b. The survival was monitored for 7 days. At the study end point, rats were anesthetized and euthanized by opening the chest cavity and withdrawing blood by cardiac puncture.
Reagents
Ultrapure LPS (Escherichia coli 0111: B4) was obtained from Sigma Chemical Co. (St. Louis, MO, USA). Rat anti-MyD88 (E-11, sc-74532), anti-TRAF6 (D-10, sc-8409), anti-IKKα (B-8, sc-7606), anti-IKKβ (G-8, sc-271782), anti-MIF (D-2, sc-271631), and anti-NF-κB (F-6, sc-8008) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). ELISA kits for CK-MB and BNP were purchased from R&D Corporation (R&D Systems Inc., Minneapolis, MN, USA). All other chemicals were of reagent grade.
Injection of LmiR-23b into Rat Hearts
The rats were anesthetized by 5.0% isoflurane and then maintained by inhalation of 1.5–2% isoflurane in 100% oxygen. Body temperature was maintained at 37 °C using a heating pad. An incision was made in the middle of the neck, and the right common carotid artery and right external carotid artery were carefully exposed. Then, a micro-catheter was introduced into the isolated common carotid artery and fed into the aortic root. One hundred microliters of LmiR-23b (1 × 108 PFU) or LmiR-Con was delivered through the micro-catheter. The micro-catheter was gently removed, and the common carotid artery was tightened before the skin was sutured. CLP was performed 7 days after delivery to initiate sepsis.
Echocardiography
Cardiac function was examined 3 days after CLP. Echocardiography was performed in rats under isoflurane anesthesia using an ultrasound system (Toshiba Aplio 80 Imaging System, Tochigi, Japan) with a 21-MHz probe to examine left ventricular function. The left ventricular ejection fraction (EF) and cardiac output (CO) were analyzed. All measurements were made by one specialist who was blinded with respect to the identity of the tracings, and analysis was performed with the average values acquired over four consecutive cardiac cycles.
Enzyme-Linked Immunosorbent Assay
The whole myocardial samples were collected and centrifuged at 3000 r/min for 10 min. After that, all samples were stored at − 80 °C until analysis. The concentrations of creatine kinase-MB (CK-MB) and brain natriuretic peptide (BNP) in serum were measured by ELISA kits (Sigma-Aldrich, St. Louis, MO, USA) following the manufacturer’s protocols.
Histological Examination
Seven days after CLP, all animals were subjected to heart perfusion under anesthesia. Then, heart specimens were obtained, fixed in 4% paraformaldehyde, dehydrated, and embedded in paraffin. Five-micron-thick sections were cut using a microtome and stained with hematoxylin and eosin (H&E) for histological examination under a light microscope (Olympus, Tokyo, Japan). A scoring system was performed as previously reported [29]. Each category was graded on a 5-point scale: 0 = no lesions, 1 = one or few small lesions, 2 = many small or a few large lesions, 3 = multiple small and large lesions, 4 = massive lesions.
Construction of miR-23b Lentivirus Expression System
miR-23b and ~ 150 bp of flanking sequence were amplified from genomic DNA and cloned into a lentivirus expression system (Invitrogen, Carlsbad, CA, USA). After sequencing confirmation, the fragment was digested with EcoRI and BamHI and cloned into lentiviral vector pCDH-CMV-MCS-EF1-copGFP (LVmiR-23b; System Biosciences, Palo Alto, CA, USA), resulting in LmiR-23b. The lentiviral miR-23b sponge (LV-sponge) was also constructed. Target cells were transfected using a polybrene reagent (Merck Millipore, Darmstadt, Germany) according to the manufacturer’s instructions. Lentivirus expressing scrambled miRNA (LmiR-Con) served as a control.
Cell Culture and Transfection
H9C2 rat cardiomyoblasts obtained from the American Type Culture Collection (Rockville, MD, USA) were cultured in RPMI-1640 medium (Gibco, Eggenstein, Germany) with 10% fetal bovine serum (FBS) supplemented with 100 U/mL penicillin and 100 U/mL streptomycin in a 5% CO2 atmosphere. The cells were plated in six-well plates at 5 × 105 cells/well and then transfected with mirVana miR-23b mimics at a final concentration of 100 nM using the transfection reagent HiPerfect (Qiagen, Hilden, Germany). Cells were treated with 100 μL LPS [10 mg of LPS in 50 mL phosphate-buffered saline (PBS)] after 7 days of transfection. For the culture experiments, the cells were divided into four groups: (1) control (medium), (2) LPS, (3) LPS + miR-23b, and (4) LPS + miR-con.
Quantitative Real-time Polymerase Chain Reaction
Total RNA was extracted from fresh rat hearts or cultured cells using an RNeasy Plus Mini Kit (Ambion, Foster City, CA, USA) according to the manufacturer’s protocol. The purity and concentration of RNA were tested at a wavelength of 260 nm using a NanoDrop 2000c spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). cDNA was synthesized with stem-loop miRNA-specific reverse transcription primers using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). qPCR was carried out on an Eppendorf Realplex 4 instrument (Eppendorf, Hamburg, Germany). miR-23b levels were quantified by qPCR using specific TaqMan assays. The specific primer sequences used for genes including TNF-α, IL-6, IL-1β, VCAM-1, ICAM-1, NF-κB, MyD88, TRAF6, IKKα, IKKβ, and β-actin were obtained from Univ-bio (Shanghai, China) and are shown in Supplementary Table 1. The relative expression of each gene was normalized to that of β-actin.
Electrophoretic Mobility Shift Assay
Nuclear proteins were isolated from heart tissues, and NF-κB binding activity was evaluated using a LightShift Chemiluminescent electrophoretic mobility shift assay (EMSA) kit (Thermo Fisher Scientific). The binding reaction mixture was incubated at room temperature for 15 min and analyzed by electrophoresis, after which the proteins were immobilized using a nylon membrane. The biotin end-labeled DNA was detected using the streptavidin-horseradish peroxidase conjugate and chemiluminescent substrate.
Western Blot
Western blot was performed as described previously [13]. In brief, collected heart tissue samples were lysed, and proteins were separated using a protein extraction kit (Well-bio, Shanghai, China) according to the manufacturer’s instructions. Membranes with diverse bands were probed with the appropriate primary antibodies after being transferred to blocking buffer (5% milk in Tris-buffered saline containing 0.05% Tween 20 [TBST]) and incubated overnight. Then, the membranes were washed and incubated with horseradish peroxidase–conjugated secondary antibody (Bio-Rad, Hercules, CA, USA). The densities of the bands on the membranes were scanned and analyzed by enhanced chemiluminescent western blotting reagents.
Flow Cytometry
After 5 days of stimulation with LPS, the H9C2 myocardiocytes were harvested and suspended, and flow cytometry was performed as previously described [30]. Cardiomyocytes were stained with Annexin-V-FITC and propidium iodide (Bioscience) for 30 min at 4 °C in the dark. The apoptotic rate of myocardiocytes was determined by flow cytometry using FACScan (BD Biosciences, San Jose, CA, USA) after 1 h.
Confocal Microscopy Analysis
Myocardiocytes were stimulated with LPS for 5 days, washed twice, and then fixed with 4% paraformaldehyde in PBS for 30 min before being permeabilized with 0.02% Triton X-100 for 30 min at 4 °C. The activity of caspase-3 was detected by confocal microscopy as in our previous study [30]. Images were acquired with a laser scanning confocal microscope (Leica, Wetzlar, Germany).
Statistical Analysis
All data were analyzed using Prism version 7.0 software (GraphPad Software, La Jolla, CA, USA). Comparisons between groups were analyzed using one-way analysis of variance (ANOVA). Survival is shown graphically using Kaplan-Meier curves and was evaluated statistically with the log-rank test. A P value less than 0.05 was considered statistically significant.
RESULTS
Polymicrobial Sepsis Increases the Level of miR-23b in the Circulation, Myocardium, and Cardiac Myocytes
To determine whether polymicrobial sepsis affects the level of miR-23b, miR-23b expression in circulation and heart tissues was investigated. The level of miR-23b in circulation and heart tissues was significantly elevated and sustained at three different time points (Fig. 1a, b). In cardiomyocytes stimulated with LPS, we found that the expression of miR-23b was also obviously increased at different phases of sepsis (24 h, 48 h, and 72 h) (Fig. 1c). These results indicated that the expression of miR-23b is upregulated by polymicrobial sepsis and possibly plays a role in sepsis.
Increased miR-23b Expression Attenuates Impaired Cardiac Dysfunction During SIC and Improves Survival Following CLP-Induced Sepsis
It has been suggested that functional rather than structural changes are involved in SIC [31]. To determine the effect of miR-23b on cardiac function in SIC, we transfected LmiR-23b or LmiR-Con into the myocardium via the right carotid artery. In the present study, upregulation of miR-23b was efficiently established in heart tissue and cardiomyocytes in vivo and in vitro, respectively (Supplementary Fig. 1A and B). Cardiac function was measured by echocardiography or ELISA 3 days after CLP, and the survival rate was carefully documented for 7 days. We found that cardiac function was severely impaired during sepsis, and LmiR-23b transfection improved cardiac dysfunction. Transfection of LmiR-23b into the myocardium improved the EF (Fig. 2a), CO (Fig. 2b), CK-MB (Fig. 2c), and BNP (Fig. 2d) during sepsis, and the survival rate (Fig. 2e) was also markedly increased compared with that in the CLP group. Furthermore, transfection of LmiR-Con did not affect CLP-induced cardiac dysfunction or mortality.
Transfection with LmiR-23b Decreases Secretion of Inflammatory Cytokines in the Myocardium
It is well known that cardiac dysfunction during SIC is likely due to excessive inflammation [32]. Therefore, the release of pro-inflammatory cytokines, such as TNF-α, IL-1β, and IL-6, and macrophage migration inhibitory factor (MIF) in the myocardium was examined by qPCR and immunoblot, respectively, 3 days after the CLP procedure. We observed that transfection with LmiR-23b significantly suppressed the secretion of sepsis-induced inflammatory cytokines, including TNF-α (Fig. 3a), IL-1β (Fig. 3b), and IL-6 (Fig. 3c). MIF is recognized as a marker of endotoxin-induced myocardial dysfunction; the data indicated that the level of MIF in the myocardium was decreased in the CLP + miR-23b group compared with that in the CLP group (Fig. 3d). However, transfection of LmiR-Con did not affect CLP-induced inflammatory cytokines.
Transfection with miR-23b Reduces the Expression of Adhesion Molecules in the Myocardium and Protects Against Histopathological Damage Following SIC
We next examined whether transfection of miR-23b influences adhesion molecule expression and histological damage in the myocardium following SIC. As shown in Fig. 4a, b, the level of vascular cell adhesion molecule 1 (VCAM-1) and intercellular cell adhesion molecule 1 (ICAM-1) in CLP rats was markedly increased compared with that in the sham group, but transfection with miR-23b prevented sepsis-induced increases in their expression in the myocardium. Furthermore, CLP-induced histopathological damage was effectively alleviated under administration of LmiR-23b (Fig. 4c, d). However, transfection of LmiR-Con did not affect adhesion molecule expression or histopathological damage.
Transfection of LmiR-23b Decreases NF-κB Binding Activity and Inhibits Activation of the NF-κB Signaling Pathway
To explore the protective mechanism by which miR-23b alleviates SIC, we assessed the NF-κB signal binding activity and related expression of NF-κB, which is considered an important transcriptional factor that regulates the secretion of inflammatory cytokines during sepsis/septic shock [33]. The results showed that the levels of NF-κB, including NF-κB binding activity (Fig. 5a), mRNA (Fig. 5b), and protein (Fig. 5c), were significantly upregulated during SIC, but transfection of miR-23b markedly decreased the levels of NF-κB, which indicated that miR-23b attenuated SIC by affecting NF-κB signaling.
Transfection of LmiR-23b Attenuates LPS-Induced H9C2 Myocardiocyte Apoptosis In Vitro
Cardiac myocyte apoptosis is considered responsible for dysfunction in SIC; thus, we detected the apoptotic rate of H9C2 cells by flow cytometry and confocal microscopy. To clarify the role of miR-23b in SIC-induced myocardiocyte apoptosis, cardiomyoblasts were treated with mirVana miR-23b mimics before LPS administration. We found that the apoptotic rate and activity of caspase-3 were significantly increased under stimulation with LPS. However, transfection of miR-23b markedly attenuated myocardial apoptosis and caspase-3 activity (Fig. 6a, b). These data indicated that upregulation of miR-23b plays a protective role in LPS-induced myocardial apoptosis.
Modulation of miR-23b Affects Activation of the NF-κB Signaling Pathway by Targeting TRAF6 and IKKβ In Vitro
To further explore the protective mechanism of transfection with LmiR-23b in SIC, we assessed the NF-κB signaling pathway in vitro. MyD88 is a critical receptor protein upstream of the signaling pathway, and TRAF6 and IKK (including IKKα and IKKβ) are essential components in NF-κB signaling pathway activation. As shown in Fig. 7, the expression of MyD88, TRAF6, and IKKβ under LPS stimulation was significantly higher than in the control but markedly decreased in the LPS + miR-23b group (Fig. 7a, b, d). However, transfection of miR-23b did not significantly affect the level of IKKα (Fig. 7c). These results suggested that induction of miR-23b during early sepsis significantly inhibits NF-κB signaling, with accompanying downregulation of MyD88, TRAF6, and IKKβ.
DISCUSSION
In present study, we found that the level of miR-23b was upregulated during SIC, and transfection with miR-23b improved survival outcome and cardiac function, prevented histological damage by inhibiting the release of inflammatory cytokines, and attenuated apoptosis of cardiomyocytes. Transfection of LmiR-23b also inhibited activation of NF-κB signaling by decreasing the level of upstream molecules. Taken together, the protective effect of enhanced miR-23b expression is mediated through MyD88-mediated NF-κB signaling and targeting of TRAF6 and IKKβ.
The cardiovascular system plays an essential role in the outcome of patients with sepsis, and a previous study provided evidence of myocardial depression in cases of septic syndromes occurring over the last half century [34]. In the pathogenesis of SIC, excessive inflammatory responses and apoptosis of cardiomyocytes serve an essential function [9, 35]. Numerous studies reported that in CLP models, early sepsis is characterized by elevated pro-inflammatory cytokine expression in the first 5 days after CLP, whereas late sepsis (after day 5) is characterized by reduced cytokine expression [36, 37]. In a recent study, miRNAs were highlighted as crucial factors in the development, homeostasis, and function of innate and adaptive immunity. In our study, we focused on miR-23b, as it represents a novel autoimmunity-regulating molecule with considerable therapeutic potential. In addition, miR-23b has been shown to inhibit inflammatory reactions in infectious diseases and attenuate vascular endothelial function during septic shock [28]. Zhang et al. reported that inhibition of miR-23b significantly improved cardiac function and contributed to the activation of cardiac fibrosis in late sepsis [38]. However, clinical and experimental studies have revealed that myocardial dysfunction is an early and fatal complication of septic shock [39, 40]. We found that the expression of miR-23b was significantly increased in the circulation, heart tissue, and H9C2 cardiomyocytes during SIC. To investigate the effect of miR-23b in early sepsis, we delivered LmiR-23b into the myocardium 7 days prior to CLP. Our study demonstrated that upregulation of miR-23b markedly improved cardiac function and survival, and the histological damage was obviously alleviated.
Inflammatory cytokines released by infiltrating macrophages and neutrophils, such as TNF-α and IL-1β, are considered important suppressors of cardiac function [41]. We found that the protective effect of miR-23b largely depended on controlling the inflammatory responses by inhibiting secretion of inflammatory cytokines (including TNF-α, IL-1β, and IL-6) in the myocardium. Increased expression of adhesion molecules, including VCAM-1 and ICAM-1, plays an essential role in mediating inflammatory cell infiltration into the tissues [41, 42]. In the present study, we observed that SIC resulted in significantly increased levels of VCAM-1 and ICAM-1 in the myocardium, but transfection of miR-23b downregulated this expression, suggesting that an increased level of miR-23b in the myocardium suppresses sepsis-induced adhesion molecule expression, resulting in attenuation of the infiltration of macrophages and neutrophils into the myocardium.
Recent evidence has supported activation of the TLR-mediated NF-κB pathway as a target of miRNAs [43], which could result in improved cardiac function and survival [44]. Ma et al. reported that cardiac function in SIC was improved by enhancement of miR-146a through targeting of the TLR-mediated NF-κB pathway [45]. Furthermore, we and others previously reported that TLR-mediated NF-κB activation contributes to the pathophysiology of polymicrobial sepsis [30] and cardiac dysfunction during SIC [46]. In the present study, we found that LmiR-23b transfection prevented sepsis-induced myocardial NF-κB binding activity and reduced the level of NF-κB. More importantly, miR-23b transfection in cardiomyocytes directly targeted TRAF6 and IKKβ, which are the key adapter molecules in the TLR/NF-κB pathway. Furthermore, TRAF6 plays a crucial role in the induction of inflammatory responses via activation of IKK, leading to NF-κB activation [32, 47]. The data revealed that miR-23b suppresses the expression of TRAF6 and IKKβ, resulting in attenuation of the NF-κB-mediated inflammatory response in SIC. Although LmiR-23b transfection did not significantly attenuate IKK-α levels in SIC, the expression of MyD88 (mRNA and protein), which functions upstream of the NF-κB pathway, was markedly decreased.
Myocardial apoptosis contributes to cardiac dysfunction in sepsis [48], and inhibition of sepsis-induced cardiac apoptosis is associated with improved cardiac function in murine polymicrobial sepsis [15]. To confirm the effect of miR-23b on apoptosis in SIC, the apoptotic rate of cardiomyocytes was measured by flow cytometry, and activity of caspase-3 was evaluated by confocal microscopy in an in vitro experiment. LmiR-23b markedly decreased the apoptotic rate of cells and activity of caspase-3 in SIC, which indicated that enhancement of miR-23b could attenuate apoptosis of H9C2 cardiomyoblasts. Taken together, the present study demonstrated that LmiR-23b transfection significantly attenuates sepsis-induced myocardial apoptosis by suppressing activation of the TLR-mediated NF-κB pathway.
There are several limitations in the present study. Although CLP is considered the gold standard model of sepsis, the healthy young rodents used herein are not representative of the elderly patients with comorbidities encountered in a clinical setting. In addition, common sepsis treatments, including antibiotics or vasoactive substances, were not used to avoid interference with the effect of miR-23b. Finally, miR-23b was transfected before CLP was performed, which is not representative of clinical practice, in which therapy is usually administered after the onset of sepsis. Further studies, particularly clinical trials, are necessary to clarify the efficacy of treatment involving miR-23b in SIC.
In conclusion, our data revealed that miR-23b is upregulated in the early stage of SIC, indicating its protective role in sepsis. Furthermore, the overexpression of miR-23b attenuated the inflammatory response and improved survival by decreasing secretion of inflammatory cytokines and alleviating myocardial apoptosis, which occurred through MyD88-mediated NF-κB signaling and targeting of TRAF6 and IKKβ. These findings, together with our previous findings, indicate the importance of understanding modulation of miR-23b in the early stage of SIC as an appropriate approach to treatment.
Data Availability
All data generated or analyzed during this study are included in this published article [and its supplementary information files].
Abbreviations
- BNP:
-
Brain natriuretic peptide
- CK-MB:
-
Creatine kinase-MB
- CLP:
-
Cecal ligation and puncture
- CO:
-
Cardiac output
- EF:
-
Ejection fraction
- ELISA:
-
Enzyme-linked immunosorbent assay
- EMSA:
-
Electrophoretic mobility shift assay
- ICAM-1:
-
Intercellular cell adhesion molecule 1
- LPS:
-
Lipopolysaccharide
- MIF:
-
Migration inhibitory factor
- miR-23b:
-
MicroRNA-23b
- NF-κB:
-
Nuclear factor kappaB
- qPCR:
-
Quantitative real-time polymerase chain reaction
- SIC :
-
Sepsis-induced cardiomyopathy
- TLR:
-
Toll-like receptor
- VCAM-1:
-
Vascular cell adhesion molecule 1
References
Singer, M., C.S. Deutschman, C.W. Seymour, M. Shankar-Hari, D. Annane, M. Bauer, R. Bellomo, G.R. Bernard, J.D. Chiche, C.M. Coopersmith, R.S. Hotchkiss, M.M. Levy, J.C. Marshall, G.S. Martin, S.M. Opal, G.D. Rubenfeld, T. van der Poll, J.L. Vincent, and D.C. Angus. 2016. The third international consensus definitions for sepsis and septic shock (sepsis-3). Journal of the American Medical Association 315: 801–810.
Gomez, E., M. Vercauteren, B. Kurtz, A. Ouvrard-Pascaud, P. Mulder, J.P. Henry, M. Besnier, A. Waget, R. Hooft Van Huijsduijnen, M.L. Tremblay, et al. 2012. Reduction of heart failure by pharmacological inhibition or gene deletion of protein tyrosine phosphatase 1B. Journal of Molecular and Cellular. 52 (6): 1257–1264.
Charpentier, J., C.E. Luyt, Y. Fulla, C. Vinsonneau, A. Cariou, S. Grabar, J.F. Dhainaut, J.P. Mira, and J.D. Chiche. 2004. Brain natriuretic peptide: a marker of myocardial dysfunction and prognosis during severe sepsis. Critical Care Medicine 32 (3): 660–665.
Gille-Johnson, P., C. Smedman, L. Gudmundsdotter, A. Somell, K. Nihlmark, S. Paulie, J. Andersson, and B. Gårdlund. 2012. Circulating monocytes are not the major source of plasma cytokines in patients with sepsis. Shock 38 (6): 577–583.
Vieillard-Baron, A., V. Caille, C. Charron, G. Belliard, B. Page, and F. Jardin. 2008. Actual incidence of global left ventricular hypokinesia in adult septic shock. Critical Care Medicine 36 (6): 1701–1706.
Fleischmann, C., A. Scherag, N.K. Adhikari, C.S. Hartog, T. Tsaganos, P. Schlattmann, D.C. Angus, and K. Reinhart. 2016. International Forum of Acute Care Trialists: Assessment of global incidence and mortality of hospital-treated Sepsis. Current estimates and limitations. American Journal of Respiratory and Critical Care Medicine 193 (3): 259–272.
Micek, S.T., C. McEvoy, M. McKenzie, N. Hampton, J.A. Doherty, and M.H. Kollef. 2013. Fluid balance and cardiac function in septic shock as predictors of hospital mortality. Critical Care 17 (5): R246.
Antonucci, E., E. Fiaccadori, K. Donadello, F.S. Taccone, F. Franchi, and S. Scolletta. 2014. Myocardial depression in sepsis: from pathogenesis to clinical manifestations and treatment. Journal of Critical Care 29 (4): 500–511.
Liu, Y.C., M.M. Yu, S.T. Shou, and Y.F. Chai. 2017. Sepsis-induced cardiomyopathy: mechanisms and treatments. Frontiers in Immunology 8 (1021).
Tsolaki, V., D. Makris, K. Mantzarlis, and E. Zakynthinos. 2017. Sepsis-induced cardiomyopathy: oxidative implications in the initiation and resolution of the damage. Oxidative Medicine and Cellular Longevity 2017: 7393525.
Suffredini, A.F., R.E. Fromm, M.M. Parker, M. Brenner, J.A. Kovacs, R.A. Wesley, and J.E. Parrillo. 1989. The cardiovascular response of normal humans to the administration of endotoxin. The New England Journal of Medicine 321 (5): 280–287.
Mann, M., A. Mehta, J.L. Zhao, K. Lee, G.K. Marinov, Y. Garcia-Flores, and D. Baltimore. 2017. An NF-κB-microRNA regulatory network tunes macrophage inflammatory responses. Nature Communications 8 (1): 851.
Cao, C., C. Yin, S. Shou, J. Wang, L. Yu, X. Li, and Y. Chai. 2018. Ulinastatin protects against LPS-induced acute lung injury by attenuating TLR4/NF-κB pathway activation and reducing inflammatory mediators. Shock 50 (5): 595–605.
Zou, L., Y. Feng, Y.J. Chen, R. Si, S. Shen, Q. Zhou, F. Ichinose, M. Scherrer-Crosbie, and W. Chao. 2010. Toll-like receptor 2 plays a critical role in cardiac dysfunction during polymicrobial sepsis. Critical Care Medicine 38 (5): 1335–1342.
Gao, M., T. Ha, X. Zhang, L. Liu, X. Wang, J. Kelley, K. Singh, R. Kao, X. Gao, D. Williams, et al. 2010. Toll-like receptor 3 plays a central role in cardiac dysfunction during polymicrobial sepsis. Critical Care Medicine 40 (8): 2390–2399.
Gao, M., T. Ha, X. Zhang, X. Wang, L. Liu, J. Kalbfleisch, K. Singh, D. Williams, and C. Li. 2013. The Toll-like receptor 9 ligand, CpG oligodeoxynucleotide, attenuates cardiac dysfunction in polymicrobial sepsis, involving activation of both phosphoinositide 3 kinase/Akt and extracellular-signal-related kinase signaling. The Journal of Infectious Diseases 207 (9): 1471–1479.
Melton, C., R.L. Judson, and R. Blelloch. 2010. Opposing microRNA families regulate self-renewal in mouse embryonic stem cells. Nature 463 (7281): 621–626.
Dvinge, H., A. Git, S. Gräf, M. Salmon-Divon, C. Curtis, A. Sottoriva, Y. Zhao, M. Hirst, J. Armisen, E.A. Miska, S.F. Chin, E. Provenzano, G. Turashvili, A. Green, I. Ellis, S. Aparicio, and C. Caldas. 2013. The shaping and functional consequences of the microRNA landscape in breast cancer. Nature 497 (7449): 378–382.
Amaral, A.E.D., M.P. Rode, J. Cisilotto, T.E.D. Silva, J. Fischer, C. Matiollo, E.C. Morais Rateke, J.L. Narciso-Schiavon, L.L. Schiavon, and T.B. Creczynski-Pasa. 2018. MicroRNA profiles in serum samples from patients with stable cirrhosis and miRNA-21 as a predictor of transplant-free survival. Pharmacological Research 134: 179–192.
Tacke, F., C. Roderburg, F. Benz, D.V. Cardenas, M. Luedde, H.J. Hippe, N. Frey, M. Vucur, J. Gautheron, A. Koch, C. Trautwein, and T. Luedde. 2014. Levels of circulating miR-133a are elevated in sepsis and predict mortality in critically ill patients. Critical Care Medicine 42 (5): 1096–1104.
Ge, C., J. Liu, and S. Dong. 2018. miRNA-214 protects sepsis-induced myocardial injury. Shock 50 (1): 112–118.
Ma, H., X. Wang, T. Ha, M. Gao, L. Liu, R. Wang, K. Yu, J.H. Kalbfleisch, R.L. Kao, D.L. Williams, and C. Li. 2016. MicroRNA-125b prevents cardiac dysfunction in polymicrobial sepsis by targeting TRAF6-mediated nuclear factor κB activation and p53-mediated apoptotic signaling. The Journal of Infectious Diseases 214 (11): 1773–1783.
Wang, H., Y. Bei, S. Shen, P. Huang, J. Shi, J. Zhang, Q. Sun, Y. Chen, Y. Yang, T. Xu, X. Kong, and J. Xiao. 2016. miR-21-3p controls sepsis-associated cardiac dysfunction via regulating SORBS2. Journal of Molecular and Cellular Cardiology 94: 43–53.
Grieco, F.A., G. Sebastiani, J. Juan-Mateu, O. Villate, L. Marroqui, L. Ladrière, K. Tugay, R. Regazzi, M. Bugliani, P. Marchetti, F. Dotta, and D.L. Eizirik. 2017. MicroRNAs miR-23a-3p, miR-23b-3p, and miR-149-5p regulate the expression of proapoptotic BH3-only proteins DP5 and PUMA in human pancreatic β-cells. Diabetes 66 (1): 100–112.
Hu, R., and R.M. O’Connell. 2012. MiR-23b is a safeguard against autoimmunity. Nature Medicine 18 (7): 1009–1010, 2017.
Zheng, J., H.Y. Jiang, J. Li, H.C. Tang, X.M. Zhang, X.R. Wang, J.T. Du, H.B. Li, and G. Xu. 2012. MicroRNA-23b promotes tolerogenic properties of dendritic cells in vitro through inhibiting Notch1/NF-κB signalling pathways. Allergy 67 (3): 362–370.
Zhu, S., W. Pan, X. Song, Y. Liu, X. Shao, Y. Tang, D. Liang, D. He, H. Wang, W. Liu, Y. Shi, J.B. Harley, N. Shen, and Y. Qian. 2012. The microRNA miR-23b suppresses IL-17-associated autoimmune inflammation by targeting TAB2, TAB3 and IKK-alpha. Nature Medicine 18 (7): 1077–1086.
Wu, M., J.T. Gu, B. Yi, Z.Z. Tang, and G.C. Tao. 2015. microRNA-23b regulates the expression of inflammatory factors in vascular endothelial cells during sepsis. Experimental and Therapeutic Medicine 9 (4): 1125–1132.
Hoyt, C.C., S.M. Richardson-Burns, R.J. Goody, B.A. Robinson, R.L. Debiasi, and K.L. Tyler. 2005. Nonstructural protein sigma1s is a determinant of reovirus virulence and influences the kinetics and severity of apoptosis induction in the heart and central nervous system. Journal of Virology 79 (5): 2743–2753.
Cao, C., C. Yin, Y. Chai, H. Jin, L. Wang, and S. Shou. 2018. Ulinastatin mediates suppression of regulatory T cells through TLR4/NF-κB signaling pathway in murine sepsis. International Immunopharmacology 64: 411–423.
Hobai, I.A., J. Edgecomb, K. LaBarge, and W.S. Colucci. 2015. Dysregulation of intracellular calcium transporters in animal models of sepsis-induced cardiomyopathy. Shock 43 (1): 3–15.
Zhang, H., H.Y. Wang, R. Bassel-Duby, D.L. Maass, W.E. Johnston, J.W. Horton, and W. Tao. 2007. Role of interleukin-6 in cardiac inflammation and dysfunction after burn complicated by sepsis. American Journal of Physiology. Heart and Circulatory Physiology 292 (5): H2408–H2416.
Zhang, G., and S. Ghosh. 2001. Toll-like receptor-mediated NF-kappaB activation: a phylogenetically conserved paradigm in innate immunity. The Journal of Clinical Investigation 107 (1): 13–19.
Sheehan, M., H.R. Wong, P.W. Hake, and B. Zingarelli. 2003. Parthenolide improves systemic hemodynamics and decreases tissue leukosequestration in rats with polymicrobial sepsis. Critical Care Medicine 31 (9): 2263–2270.
Zheng, Z., H. Ma, X. Zhang, F. Tu, X. Wang, T. Ha, M. Fan, L. Liu, J. Xu, K. Yu, R. Wang, J. Kalbfleisch, R. Kao, D. Williams, and C. Li. 2017. Enhanced glycolytic metabolism contributes to cardiac dysfunction in polymicrobial sepsis. The Journal of Infectious Diseases 215 (9): 1396–1406.
Brudecki, L., D.A. Ferguson, D. Yin, G.D. Lesage, C.E. McCall, and M. El Gazzar. 2012. Hematopoietic stem-progenitor cells restore immunoreactivity and improve survival in late sepsis. Infection and Immunity 80 (2): 602–611.
Yoon, S.J., S.J. Kim, and S.M. Lee. 2017. Overexpression of HO-1 contributes to sepsis-induced immunosuppression by modulating the Th1/Th2 balance and regulatory T-cell function. The Journal of Infectious Diseases 215 (10): 1608–1618.
Zhang, H., Y. Caudle, A. Shaikh, B. Yao, and D. Yin. 2018. Inhibition of microRNA-23b prevents polymicrobial sepsis-induced cardiac dysfunction by modulating TGIF1 and PTEN. Biomedicine & Pharmacotherapy 103: 869–878.
Court, O., A. Kumar, J.E. Parrillo, and A. Kumar. 2002. Clinical review: Myocardial depression in sepsis and septic shock. Critical Care 6 (6): 500–508.
Chagnon, F., C.N. Metz, R. Bucala, and O. Lesur. 2005. Endotoxin-induced myocardial dysfunction: effects of macrophage migration inhibitory factor neutralization. Circulation Research 96 (10): 1095–1102.
Alves-Filho, J.C., A. de Freitas, F. Spiller, F.O. Souto, and C.Q. Cunha. 2008. The role of neutrophils in severe sepsis. Shock 30: 3–9.
Cavaillon, J.M., and M. Adib-Conquy. 2005. Monocytes/macrophages and sepsis. Critical Care Medicine 33: S506–S509.
O’Neill, L.A., F.J. Sheedy, and C.E. McCoy. 2011. MicroRNAs: the fine-tuners of Toll-like receptor signalling. Nature Reviews. Immunology 11 (3): 163–175.
Williams, D.L., T. Ha, C. Li, J.H. Kalbfleisch, J. Schweitzer, W. Vogt, and I.W. Browder. 2003. Modulation of tissue Toll-like receptor 2 and 4 during the early phases of polymicrobial sepsis correlates with mortality. Critical Care Medicine 31 (6): 1808–1818.
Gao, M., X. Wang, X. Zhang, T. Ha, H. Ma, L. Liu, J.H. Kalbfleisch, X. Gao, R.L. Kao, D.L. Williams, and C. Li. 2015. Attenuation of cardiac dysfunction in polymicrobial sepsis by microRNA-146a is mediated via targeting of IRAK1 and TRAF6 expression. Journal of Immunology 195 (2): 672–682.
Ha, T., C. Lu, L. Liu, F. Hua, Y. Hu, J. Kelley, K. Singh, R.L. Kao, J. Kalbfleisch, D.L. Williams, X. Gao, and C. Li. 2010. TLR2 ligands attenuate cardiac dysfunction in polymicrobial sepsis via a phosphoinositide 3-kinase-dependent mechanism. American Journal of Physiology. Heart and Circulatory Physiology 298 (3): H984–H991.
Medzhitov, R., P. Preston-Hurlburt, and C.A.J. Janeway. 1997. A human homologue of the Drosophila Toll protein signals activation of adaptive immunity. Nature 388 (6640): 394–397.
Nevière, R., H. Fauvel, C. Chopin, P. Formstecher, and P. Marchetti. 2001. Caspase inhibition prevents cardiac dysfunction and heart apoptosis in a rat model of sepsis. American Journal of Respiratory and Critical Care Medicine 163 (1): 218–225.
Funding
This work was supported by the National Natural Science Foundation of China (Grant No. 81871593 to YFC), Theory E Emergency Medical Research Fund of China (Grant No. R2015026 to CC), and Tianjin Medical University General Hospital Fund of China (Grant No. ZYYFY2015010 to CC, ZYYFY2016026 to YZ).
Author information
Authors and Affiliations
Contributions
CC performed experiments, analyzed data, prepared figures, and wrote the manuscript. YZ, LJW, YFC, and STS performed experiments and analyzed data. CFY performed the histological examination of the heart tissues. HJ designed experiments, analyzed data, prepared figures, and wrote the manuscript. All authors read and approved the final manuscript.
Corresponding author
Ethics declarations
Competing Interests
The authors declare that they have no conflicts of interest.
Ethics Approval and Consent to Participate
All experimental manipulations were undertaken in accordance with the Guide for the Care and Use of Medical Laboratory Animals (Ministry of Health, P.R. China, 1998), with the approval of the Scientific Investigation Board, Tianjin Medical University General Hospital, Tianjin, China.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Cao, C., Zhang, Y., Chai, Y. et al. Attenuation of Sepsis-Induced Cardiomyopathy by Regulation of MicroRNA-23b Is Mediated Through Targeting of MyD88-Mediated NF-κB Activation. Inflammation 42, 973–986 (2019). https://doi.org/10.1007/s10753-019-00958-7
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10753-019-00958-7