Abstract
Podophyllum hexandrum Royle is an important medicinal herb of North-Western Himalayas, and podophyllotoxin, being its major metabolite, has been used extensively in the preparation of several anticancer drugs. Podophyllotoxin accumulates in rhizomes; however, no information exists on the role of ATP-binding cassette (ABC) transporters vis-à-vis podophyllotoxin content. The present study reports identification, validation, and expression analysis of ABC transporter genes from P. hexandrum. Total 252 ABC transporter genes were identified as unigenes out of which 22 were further validated using real time qPCR in different tissues of varying podophyllotoxin content. Differential expression analysis and Pearson’s correlation coefficient revealed two candidate genes PhABC6 and PhABCIII having a positive correlation with the podophyllotoxin content. PhABCIV showed the highest expression in rhizomes (20.53-folds compared to shoots) suggesting its possible role in transport and accumulation of podophyllotoxin.
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Abbreviations
- ABC:
-
ATP-binding cassette
- miRNA:
-
microRNA
- FPKM:
-
fragments per kilobase of transcript per million mapped reads
- NGS:
-
next-generation sequencing
- PERL:
-
Practical Extraction and Report Language
- Pfam:
-
protein family database
- qPCR:
-
quantitative polymerase chain reaction
- RSEM:
-
RNA sequences by expectation maximization
- TFs:
-
transcription factors
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The authors are thankful to the Jaypee University of Information Technology for providing the necessary research facilities. The authors are also thankful to the Department of Biotechnology, the Ministry of Science & Technology, Govt. of India for a grant to RSC, and to the Himalayan Forest Research Institute, Panthaghati, Shimla, India for providing the plant material of P. hexandrum. The work presented here was carried out in collaboration of all authors.
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Kumar, P., Sharma, R., Jaiswal, V. et al. Identification, validation, and expression of ABC transporters in Podophyllum hexandrum and their role in podophyllotoxin biosynthesis. Biol Plant 60, 452–458 (2016). https://doi.org/10.1007/s10535-016-0611-9
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DOI: https://doi.org/10.1007/s10535-016-0611-9