A new automated method for assaying specific agglutination of red blood cells upon addition of monoclonal antibodies to whole blood test samples is presented. The method can be used for blood type determination. The method is based on lying drop photometry of blood samples with added Tsoliclone monoclonal antibodies. Mechanical vibration of drop samples promotes better cell agglutination and redistribution through the volume of the sample. Experimental studies revealed significant differences in photometric values for samples with and without agglutination. This method may provide the basis for developing a portable automatic blood-typing device.
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Translated from Meditsinskaya Tekhnika, Vol. 55, No. 5, Sep.-Oct., 2021, pp. 19-23.
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Aristov, A.A., Rozenbaum, Y.A. & Evtushenko, G.S. An Automated Method for Blood Type Determination by Red Blood Cell Agglutination Assay. Biomed Eng 55, 328–332 (2022). https://doi.org/10.1007/s10527-022-10129-y
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DOI: https://doi.org/10.1007/s10527-022-10129-y