Hydroxyapatite was synthesized in conditions of mechanoacoustic treatment of an aqueous reaction mixture consisting of ammonium hydrogen phosphate and calcium nitrate using a commercially available rotary pulsation apparatus. Mechanoacoustic treatment of the reaction mixture was found to lead to the formation of nanosized hydroxyapatite particles with a predominant mean diameter of about 20 nm. Purification of technical hydroxyapatite to remove traces of ammonium nitrate by thermal processing at 350°C is proposed. The crystalline phase of hydroxyapatite was found not to be dominant in the structure. The porosity of hydroxyapatite amounted to about 75% with a mean pore diameter of 2.8 × 103 nm. Thermal processing of hydroxyapatite in aqueous medium was found to yield a paste-like form. Hydroxyapatite paste had no cytotoxicity and did not prevent cell adhesion, though it almost completely inhibited cell spreading.
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Translated from Meditsinskaya Tekhnika, Vol. 52, No. 1, Jan.-Feb., 2018, pp. 13-16.
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Teleshev, A.T., Gorshenev, V.N., Yakovleva, M.A. et al. Hydroxyapatite for Filling Bone Tissue Defects. Biomed Eng 52, 19–22 (2018). https://doi.org/10.1007/s10527-018-9773-4
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DOI: https://doi.org/10.1007/s10527-018-9773-4