Late diagnosis of ovarian cancer is one of the most important problems in its treatment. Long non-coding RNA (lncRNA) are a poorly studied, but promising type of diagnostic biomarkers. We studied the lncRNA interactome to identify biomarkers with potential significance for molecular diagnostics of ovarian cancer. By screening the TCGA database, we identified differentially expressed lncRNA CCAT1 and SNHG14. Based on the indices of complementarity of CCAT1 and SNHG14 to the mRNA sequences, we selected 5 protein-coding genes MAPK1, c-MET, TGFB2, SNAIL1, and WNT4 associated with the epithelial-mesenchymal transition. Real-time PCR on 54 ovarian cancer samples confirmed the high expression levels of CCAT1 and SNHG14 (logFC>1.5, p<0.05). A positive correlation between the expression levels of two lncRNA and mRNA of 5 genes in 6 pairs was established. The activating effect of CCAT1 and SNHG14 on the expression of these genes can be mediated by miR-203 and miR-124.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Brovkina OI, Pronina IV, Uroshlev LA, Loginov VI, Braga EA, Fridman MV, Kazubskaya TP, Utkin DO, Kushlinskii NE. Identification of novel differentially expressing long non-coding RNAs with oncogenic potential. Mol. Biol. 2021;55(4):548-554. doi: https://doi.org/10.1134/S0026893321020175
Filippova EA, Burdennyy AM, Lukina SS, Ivanova NA, Pronina IV, Kazubskaya TP, Braga EA, Loginov VI. Changes in the methylation level of microRNA genes as a factor of breast cancer development and progression. Patol. Fiziol. Eksp. Ter. 2021;65(3):4-11. doi: https://doi.org/10.25557/0031-2991.2021.03.4-11.Russian.
Cao Y, Shi H, Ren F, Jia Y, Zhang R. Long non-coding RNA CCAT1 promotes metastasis and poor prognosis in epithelial ovarian cancer. Exp. Cell Res. 2017;359(1):185-194. doi: https://doi.org/10.1016/j.yexcr.2017.07.030
Deng L, Yang SB, Xu FF, Zhang JH. Long noncoding RNA CCAT1 promotes hepatocellular carcinoma progression by functioning as let-7 sponge. J. Exp. Clin. Cancer Res. 2015;34(1):18. doi: https://doi.org/10.1186/s13046-015-0136-7
Liu Z, Chen Q, Hann SS. The functions and oncogenic roles of CCAT1 in human cancer. Biomed. Pharmacother. 2019;115:108943. doi: https://doi.org/10.1016/j.biopha.2019.108943
Loginov VI, Pronina IV, Burdennyy AM, Filippova EA, Kazubskaya TP, Kushlinsky DN, Utkin DO, Khodyrev DS, Kushlinskii NE, Dmitriev AA, Braga EA. Novel miRNA genes deregulated by aberrant methylation in ovarian carcinoma are involved in metastasis. Gene. 2018;662:28-36. doi: https://doi.org/10.1016/j.gene.2018.04.005
Ribatti D, Tamma R, Annese T. Epithelial-mesenchymal transition in cancer: a historical overview. Transl. Oncol. 2020;13(6):100773. doi: https://doi.org/10.1016/j.tranon.2020.100773
Stemmler MP, Eccles RL, Brabletz S, Brabletz T. Non-redundant functions of EMT transcription factors. Nat. Cell Biol. 2019;21(1):102-112. doi: https://doi.org/10.1038/s41556-018-0196-y
Sun X, Shen H, Liu S, Gao J, Zhang S. Long noncoding RNA SNHG14 promotes the aggressiveness of retinoblastoma by sponging microRNA-124 and thereby upregulating STAT3. Int. J. Mol. Med. 2020;45(6):1685-1696. doi: https://doi.org/10.3892/ijmm.2020.4547
Vasaikar SV, Deshmukh AP, den Hollander P, Addanki S, Kuburich NA, Kudaravalli S, Joseph R, Chang JT, Soundararajan R, Mani SA. EMTome: a resource for pan-cancer analysis of epithelial-mesenchymal transition genes and signatures. Br. J. Cancer. 2021;124(1):259-269. doi: https://doi.org/10.1038/s41416-020-01178-9
Wang JY, Lu AQ, Chen LJ. LncRNA in ovarian cancer. Clin. Chim. Acta. 2019;490:17-27. doi: https://doi.org/10.1016/j.cca.2018.12.013
WHO Classification of Tumours of Female Reproductive Organs. Kurman R, Carcangiu ML, Herrington CS, Young RH, eds. Lyon, 2014.
Xiang JF, Yin QF, Chen T, Zhang Y, Zhang XO, Wu Z, Zhang S, Wang HB, Ge J, Lu X, Yang L, Chen LL. Human colorectal cancer-specific CCAT1-L lncRNA regulates longrange chromatin interactions at the MYC locus. Cell Res. 2014;24(5):513-531. doi: https://doi.org/10.1038/cr.2014.35
Xu X, Song F, Jiang X, Hong H, Fei Q, Jin Z, Zhu X, Dai B, Yang J, Sui C, Xu M. Long non-coding RNA SNHG14 contributes to the development of hepatocellular carcinoma via sponging miR-217. Onco Targets Ther. 2020;13:4865-4876. doi: https://doi.org/10.2147/OTT.S244530
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 172, No. 12, pp. 764-768, December, 2021
Rights and permissions
About this article
Cite this article
Brovkina, O.I., Pronina, I.V., Burdennyy, A.M. et al. The Role of Long Non-Coding RNA CCAT1 and SNHG14 in Activation of Some Protein-Coding Genes Associated with the Development of Ovarian Cancer. Bull Exp Biol Med 172, 760–764 (2022). https://doi.org/10.1007/s10517-022-05473-8
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-022-05473-8