A comparative analysis of the parameters characterizing sperm apoptosis of young (27-42 years) and middle-aged (44-51 years) men was performed by flow cytometry. Irrespective of age, activity of caspase-3 and p53-mediated controlling the transmission of apoptogenic signal transmission in gametes remained stable with the formation of germ cells with delayed (p<0.05) cell death according to the Annexin V-FITC+PI— criterion (predominantly in middle-aged men). Inhibition of the transmission of a proapoptogenic stimulus mediated by membrane cell death receptors (FAS) was also observed in this group. Comparison of indicators of sperm apoptosis showed age-related features of cell death, in particular, inhibition of membrane reception triggering FAS-dependent apoptosis, which is associated with insufficient phosphatidylserine production in middle-aged men, excessive life cycle duration, and aging of spermatozoa.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 172, No. 10, pp. 501-504, October, 2021
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Dolgikh, O.V., Dianova, D.G., Krivtsov, A.V. et al. Comparative Evaluation of the Parameters of Sperm Apoptosis of Young and Middle-Aged Men by Flow Cytometry. Bull Exp Biol Med 172, 483–485 (2022). https://doi.org/10.1007/s10517-022-05418-1
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DOI: https://doi.org/10.1007/s10517-022-05418-1