In cultures of peritoneal macrophages (MP) of male BALB/c mice infected with Mycobacterium tuberculosis from the BCG vaccine, the expression of CD1, CD14, CD25, CD30, CD35, and CD95 receptors was studied in vitro 3 months after infection. In MP cultures from intact and infected mice, mononuclear MP predominated (96 and 92%, respectively). Bi- and trinuclear MP in MP cultures from control and infected mice constituted 4 and 8.3% of all MP, respectively. In the cultures of both groups, no obvious correlations between the number of MP expressing CD-receptors and number of nuclei in these cells were found, but the expression of CD14 receptor was more often noted. In cultures from infected animals, hypertrophied MP and enhanced (by several times) expression of all CD-receptors were observed. The increase in the expression of CD-receptor can be determined by activation of plastic processes in hypertrophied MP (in epithelioid and in numerically insignificant polynuclear MP), which is due to the phenomenon of prolonged M. tuberculosis persistence in the vacuolar apparatus of these cells.
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Iljine DA, Arkhipov SA, Shkurupy VA. In vitro expression of IL-1α, GM-CSF, and TNF-α by multinucleated macrophages from BCG-infected mice. Bull. Exp. Biol. Med. 2013;155(5):663-666. doi: https://doi.org/10.1007/s10517-013-2220-3
Il’in DA, Arkhipov SA, Shkurupy VA. In Vitro Study of Cytophysiological Characteristics of Multinuclear Macrophages from Intact and BCG-Infected Mice. Bull. Exp. Biol. Med. 2016;160(5):668-71. doi: https://doi.org/10.1007/s10517-016-3245-1
Il’in DA, Arkhipov SA, Shkurupy VA. Analysis of IL-1α, bFGF, TGF-β1, IFNγ, MMP-1, and CatD Expression in Multinuclea Macrophages In Vitro. Bull. Exp. Biol. Med. 2018;164(4):456-458. doi: https://doi.org/10.1007/s10517-018-4011-3
Il’in DA, Shkurupy VA. Expression of Pro- and Antiapoptotic Factors in Multinuclear Macrophages of BCG-Infected Mice In Vitro. Bull. Exp. Biol. Med. 2018;165(4):482-485. doi: https://doi.org/10.1007/s10517-018-4199-2
Neshchadim DV, Arkhipov SA, Shkurupy VA, Akhramenko ES, Troitskii AV, Karpov MA. Modifying Effects of Nanosized Diamonds on Hydrolytic Potential of Macrophages In Vitro. Bull. Exp. Biol. Med. 2015;159(3):398-401. doi: https://doi.org/10.1007/s10517-015-2973-y
Shkurupy VA. Tuberculous Granulomatosis. Cytophysiology and Target Treatment. Moscow, 2007. Russian.
Shkurupy VA, Ilin DA, Arkhipov SA. Polynuclearity phenomenon — macrophage response to M. tuberculosis persistence in macrophages. Mezhdunarod. Zh. Priklad. Fundament. Issled. 2017;(11-2):218-222. Russian.
Shkurupii VA, Kim LB, Potapova OV, Sharkova TV, Putyatina AN, Nikonova IK. Study of fibrotic complications and hydroxyproline content in mouse liver at different stages of generalized BCG-induced granulomatosis. Bull. Exp. Biol. Med. 2014;157(4):466-469. doi: https://doi.org/10.1007/s10517-014-2592-z
Shkurupii VA, Kozyaev MA, Nadeev AP. Ultrastructural characteristics of type A epithelioid cells during BCG-granulomatosis and treatment with lysosomotropic isoniazid. Bull. Exp. Biol. Med. 2006;141(4):487-490. doi: https://doi.org/10.1007/s10517-006-0206-0
Anand D, Kumar U, Kanjilal M, Kaur S, Das N. Leucocyte complement receptor 1 (CR1/CD35) transcript and its correlation with the clinical disease activity in rheumatoid arthritis patients. Clin. Exp. Immunol. 2014;176(3):327-335. doi: https://doi.org/10.1111/cei.12274
Ayaslioglu E, Kalpaklioglu F, Kavut A. B, Erturk A, Capan N, Birben E. The role of CD14 gene promoter polymorphism in tuberculosis susceptibility. J. Microbiol. Immunol. Infect. 2013;46(3):158-163. doi: https://doi.org/10.1016/j.jmii.2012.05.008
Fehlings M, Drobbe L, Moos V, Renner Viveros P, Hagen J, Beigier-Bompadre M, Pang E, Belogolova E, Churin Y, Schneider T, Meyer TF, Aebischer T, Ignatius R. Comparative analysis of the interaction of Helicobacter pylori with human dendritic cells, macrophages, and monocytes. Infect. Immun. 2012;80(8):2724-2734. doi: https://doi.org/10.1128/IAI.00381-12
Munk ME, Kern P, Kaufmann SH. Human CD30+ cells are induced by Mycobacterium tuberculosis and present in tuberculosis lesions. Int. Immunol. 1997;9(5):713-720. doi: https://doi.org/10.1093/intimm/9.5.713
Tchikov V, Bertsch U, Fritsch J, Edelmann B, Schütze S. Subcellular compartmentalization of TNF receptor-1 and CD95 signaling pathways. Eur. J. Cell Biol. 2011;90(6-7):467-475. doi: https://doi.org/10.1016/j.ejcb.2010.11.002
Van Kaer L, Wu L, Joyce S. Mechanisms and consequences of antigen presentation by CD1. Trends Immunol. 2016;37(11):738-754. doi: https://doi.org/10.1016/j.it.2016.08.011
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 172, No. 7, pp. 52-55, July, 2021
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Il’in, D.A., Shkurupy, V.A. & Akhramenko, E.S. In Vitro Study of the Expression of CD1, CD14, CD25, CD30, CD35, CD95 Receptors by Macrophages of Mice Infected with Mycobacterium tuberculosis. Bull Exp Biol Med 172, 42–45 (2021). https://doi.org/10.1007/s10517-021-05327-9
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DOI: https://doi.org/10.1007/s10517-021-05327-9