Immunogenic and protective activity of recombinant pneumolysin was studied in experiments on male BALB/c mice. The mice were immunized intraperitoneally with recombinant pneumolysin sorbed on Al(OH)3 (200 μg per mouse). In 2 weeks after immunization, the isotypes of antibodies to recombinant pneumolysin in the serum of immunized mice were determined by ELISA. The animals were infected with Streptococcus pneumoniae serotype 3. Immunization with recombinant pneumolysin induced the production of anti-pneumolysin antibodies, mainly of IgG1 subisotype. On day 21 after intraperitoneal infection with S. pneumoniae serotype 3 in a dose of 106 microbial cells, the survival rate of animals immunized with recombinant pneumolysin in a dose of 25 μg/mouse was 67% vs. 0% in the control (p<0.001). Recombinant pneumolysin could be considered as a promising protective antigen for inclusion in the serotype-independent vaccine against S. pneumoniae.
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References
Glantz SA. Primer of Biostatistics. Moscow, 1999. Russian.
Anderson R, Nel JG, Feldman C. Multifaceted Role of Pneumolysin in the Pathogenesis of Myocardial Injury in Community- Acquired Pneumonia. Int. J. Mol. Sci. 2018;19(4). pii: E1147. doi: https://doi.org/10.3390/ijms19041147.
Jackson LA, Janoff EN. Pneumococcal vaccination of elderly adults: new paradigms for protection. Clin. Infect. Dis. 2008;47(10):1328-1338.
Moffitt K, Malley R. Rationale and prospects for novel pneumococcal vaccines. Hum. Vaccin. Immunother. 2016;12(2):383-392.
Sings HL. Pneumococcal conjugate vaccine use in adults – Addressing an unmet medical need for non-bacteremic pneumococcal pneumonia. Vaccine. 2017;35(40):5406-5417.
Vaneeva NP, Yastrebova NE. Specific immune response to certain capsule polysaccharides of Streptococcus pneumoniae in healthy blood donors and individuals immunized with pneumococcal vaccines. Zh. Mikrobiol. Epidemiol. Immunobiol. 2015;(5):20-26.
Weiser JN, Ferreira DM, Paton JC. Streptococcus pneumoniae: transmission, colonization and invasion. Nat. Rev. Microbiol. 2018;16(6):355-367.
Yahiaoui RY, Bootsma HJ, den Heijer CDJ, Pluister GN, John Paget W, Spreeuwenberg P, Trzcinski K, Stobberingh EE. Distribution of serotypes and patterns of antimicrobial resistance among commensal Streptococcus pneumoniae in nine European countries. BMC Infect. Dis. 2018;18(1):440. doi: https://doi.org/10.1186/ s12879-018-3341-0.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 168, No. 10, pp. 471-473, October, 2019
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Petukhova, E.S., Vorobyev, D.S., Sidorov, A.V. et al. Immunization with Recombinant Pneumolysin Induces the Production of Antibodies and Protects Mice in a Model of Systemic Infection Caused by Streptococcus pneumoniae. Bull Exp Biol Med 168, 485–487 (2020). https://doi.org/10.1007/s10517-020-04736-6
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DOI: https://doi.org/10.1007/s10517-020-04736-6