Intracerebral transplantation of mesenchymal stem cells to 6- and 12-month-old SHR rats induced angiogenesis in the pia mater. In 6-months-old SHR rats, perfusion in the brain tissue after cell transplantation considerably increased, while in 12-month-old rats it remained practically unchanged. We also observed marked activation of regulatory processes in the cerebral vascular system, most pronounced in 12-month-old rats. Neurogenic and myogenic tone of cerebral vessels increased significantly, while endothelium-dependent tone slightly decreased. The increase in neurogenic and myogenic tone of blood vessels in SHR rats at the age of 6 and 12 months after transplantation of stem cells can be explained by the formation of new smooth muscle cells in the pre-existing arteries walls. Greater muscle mass developed stronger force and contributed to narrowing of the arterial lumen, as a result, there was no increase in blood flow despite the downstream angiogenesis. A slight decrease in endothelium-dependent tone can be explained by increased production of vasodilators by newly formed endothelial cells.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Lobov GI, Gurkov AS. Modulation of blood flow in the microvasculature of fingers after the formation a radiocephalic arteriovenous fistula. Nefrologiya Dializ. 2014;16(3):364-371. Russian.
Sokolova IB, Sergeev IV, Skorobogataya EV, Ufimtseva AN, Polyntsev DG, Dvoretskii DP. Effect of Transplantation of Mesenchymal Stem Cells on the Density of Pial Microvascular Network in Spontaneously Hypertensive Rats of Different Age. Bull. Exp. Biol. Med. 2017;163(1):129-132.
Armulik A, Abramsson A, Betsholtz C. Endothelial/pericyte interactions. Circ. Res. 2005;97(6):512-523.
Bronckaers A, Hilkens P, Martens W, Gervois P, Ratajczak J, Struys T, Lambrichts I. Mesenchymal stem/stromal cells as a pharmacological and therapeutic approach to accelerate angiogenesis. Pharmacol. Ther. 2014;143(2):181-196.
da Silva Meirelles L, Caplan AI, Nardi NB. In search of the in vivo identity of mesenchymal stem cells. Stem Cells. 2008;26(9):2287-2299.
de Oliveira LF, Almeida TR, Ribeiro Machado MP, Cuba MB, Alves AC, da Silva MV, Rodrigues Júnior V, Dias da Silva VJ. Priming mesenchymal stem cells with endothelial growth medium boosts stem cell therapy for systemic srterial hypertension. Stem Cells Int. 2015;2015. ID 685383. doi: https://doi.org/10.1155/2015/685383.
Heinert G, Casadei B, Paterson DJ. Hypercapnic cerebral blood flow in spontaneously hypertensive rats. J. Hypertens. 1998;16(10):1491-1498.
Lee TH, Liu HL, Yang ST, Yang JT, Yeh MY, Lin JR. Effects of aging and hypertension on cerebral ischemic susceptibility: evidenced by MR diffusion-perfusion study in rat. Exp. Neurol. 2011;227(2):314-321.
Li Y, Shen Q, Huang S, Li W, Muir ER, Long JA, Duong TQ. Cerebral angiography, blood flow and vascular reactivity in progressive hypertension. Neuroimage. 2015;111:329-337.
Meneses A, Hong E. Spontaneously hypertensive rats: a potential model to identify drugs for treatment of learning disorders. Hypertension. 1998 Vol. 31(4):968-972.
Sabbatini M, Strocchi P, Vitaioli L, Amenta F. Microanatomical changes of intracerebral arteries in spontaneously hypertensive rats: a model of cerebrovascular disease of the elderly. Mech. Ageing Dev. 2001;122(12):1257-1268.
Tayebati SK, Tomassoni D, Amenta F. Spontaneously hypertensive rat as a model of vascular brain disorder: microanatomy, neurochemistry and behavior. J. Neurol. Sci. 2012;322(1-2):241-249.
Touyz RM. Reactive oxygen species, vascular oxidative stress, and redox signaling in hypertension: what is the clinical significance? Hypertension. 2004;44(3):248-252.
Wang L, Mu Z, Lin X, Geng J, Xiao TQ, Zhang Z, Wang Y, Guan Y, Yang GY. Simultaneous imaging of cerebrovascular structure and function in hypertensive rats using synchrotron radiation angiography. Front. Aging Neurosci. 2017;9:359. doi: https://doi.org/10.3389/fnagi.2017.00359.
Zhou Q, Dong Y, Huang L, Yang S, Chen W. Study of cerebrovascular reserve capacity by magnetic resonance perfusion weighted imaging and photoacoustic imaging. Magn. Reson. Imaging. 2009;27(2):155-162.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 275-280, December, 2018
Rights and permissions
About this article
Cite this article
Sokolova, I.B., Lobov, G.I. Cerebral Blood Flow in SHR Rats after Transplantation of Mesenchymal Stem Cells. Bull Exp Biol Med 166, 586–590 (2019). https://doi.org/10.1007/s10517-019-04396-1
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-019-04396-1