Production of microvesicles in culture of human umbilical cord multipotent mesenchymal stromal cells was studied and comparative analysis of the expression of some surface molecules (clusters of differentiation, CD) was performed. It was found that the mesenchymal stromal cells produce microvesicles in the amount sufficient for their detection by flow cytometry. Parallel analysis of the phenotypes of maternal mesenchymal stromal cells and secreted microvesicles revealed identical expression of surface molecules CD13, CD29, CD44, CD54, CD71, CD73, CD90, CD105, CD106, and HLA-I. The concentration of microvesicles in the conditioned medium was 17.9±4.6×106/ml; i.e. one cell produced ~40-50 (44.7±11.5) microvesicles over 2 days in culture.
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Arno AI, Amini-Nik S, Blit PH, Al-Shehab M, Belo C, Herer E, Tien CH, Jeschke MG. Human Wharton’s jelly mesenchymal stem cells promote skin wound healing through paracrine signaling. Stem Cell Res. Ther. 2014;5(1):28. doi: https://doi.org/10.1186/scrt417.
Batsali AK, Kastrinaki MC, Papadaki HA, Pontikoglou C. Mesenchymal stem cells derived from Wharton’s Jelly of the umbilical cord: biological properties and emerging clinical applications. Curr. Stem Cell Res. Ther. 2013;8(2):144-155.
Can A, Celikkan FT, Cinar O. Umbilical cord mesenchymal stromal cell transplantations: a systemic analysis of clinical trials. Cytotherapy. 2017;19(12):1351-1382.
Carvalho MM, Teixeira FG, Reis RL, Sousa N, Salgado AJ. Mesenchymal stem cells in the umbilical cord: phenotypic characterization, secretome and applications in central nervous system regenerative medicine. Curr. Stem Cell Res. Ther. 2011;6(3):221-228.
Corrao S, La Rocca G, Lo Iacono M, Zummo G, Gerbino A, Farina F, Anzalone R. New frontiers in regenerative medicine in cardiology: the potential of Wharton’s jelly mesenchymal stem cells. Curr. Stem Cell Res. Ther. 2013;8(1):39-45.
Davies JE, Walker JT, Keating A. Concise Review: Wharton’s Jelly: the rich, but enigmatic, source of mesenchymal stromal cells. Stem Cells Transl. Med. 2017;6(7):1620-1630.
Detamore MS. Human umbilical cord mesenchymal stromal cells in regenerative medicine. Stem Cell Res. Ther. 2013;4(6):142. doi: https://doi.org/10.1186/scrt353.
Ding DC, Chang YH, Shyu WC, Lin SZ. Human umbilical cord mesenchymal stem cells: a new era for stem cell therapy. Cell Transplant. 2015;24(3):339-347.
He H, Nagamura-Inoue T, Takahashi A, Mori Y, Yamamoto Y, Shimazu T, Tsunoda H, Tojo A. Immunosuppressive properties of Wharton’s jelly-derived mesenchymal stromal cells in vitro. Int. J. Hematol. 2015;102(3):368-378.
Hsieh JY, Wang HW, Chang SJ, Liao KH, Lee IH, Lin WS, Wu CH, Lin WY, Cheng SM. Mesenchymal stem cells from human umbilical cord express preferentially secreted factors related to neuroprotection, neurogenesis, and angiogenesis. PLoS One. 2013;8(8):e72604. doi: https://doi.org/10.1371/journal.pone.0072604.
Kalaszczynska I, Ferdyn K. Wharton’s jelly derived mesenchymal stem cells: future of regenerative medicine? Recent findings and clinical significance. Biomed. Res. Int. 2015;2015. ID 430847. doi: https://doi.org/10.1155/2015/430847.
Kamolz LP, Keck M, Kasper C. Wharton’s jelly mesenchymal stem cells promote wound healing and tissue regeneration. Stem Cell Res. Ther. 2014;5(3):62. doi: https://doi.org/10.1186/scrt451.
Ophelders DR, Wolfs TG, Jellema RK, Zwanenburg A, Andriessen P, Delhaas T, Ludwig AK, Radtke S, Peters V, Janssen L, Giebel B, Kramer BW. Mesenchymal stromal cell-derived extracellular vesicles protect the fetal brain after hypoxia-ischemia. Stem Cells Transl. Med. 2016;5(6):754-763.
Pashoutan Sarvar D, Shamsasenjan K, Akbarzadehlaleh P. Mesenchymal stem cell-derived exosomes: new opportunity in cell-free therapy. Adv. Pharm. Bull. 2016;6(3):293-299.
Ribeiro J, Gartner A, Pereira T, Gomes R, Lopes MA, Gonçalves C, Varejão A, Luís AL, Maurício A.C. Perspectives of employing mesenchymal stem cells from the Wharton’s jelly of the umbilical cord for peripheral nerve repair. Int. Rev. Neurobiol. 2013;108:79-120.
Romanov YA, Balashova EE, Volgina NE, Kabaeva NV, Dugina TN, Sukhikh GT. Optimized protocol for isolation of multipotent mesenchymal stromal cells from human umbilical cord. Bull. Exp. Biol. Med. 2015;160(1):148-154.
Romanov YA, Balashova EE, Volgina NE, Kabaeva NV, Dugina TN, Sukhikh GT. Human umbilical cord blood serum: effective substitute of fetal bovine serum for culturing of human multipotent mesenchymal stromal cells. Bull. Exp. Biol. Med. 2017;162(4):528-533.
Romanov YA, Balashova EE, Volgina NE, Kabaeva NV, Dugina TN, Sukhikh GT. Expression of surface molecules in human mesenchymal stromal cells co-cultured with nucleated umbilical cord blood cells. Bull. Exp. Biol. Med. 2017;162(4):578-582.
Romanov YA, Volgina NE, Balashova EE, Kabaeva NV, Dugina TN, Sukhikh GT. Human umbilical cord mesenchymal stromal cells support viability of umbilical cord blood hematopoietic stem cells but not the “stemness” of their progeny in co-culture. Bull. Exp. Biol. Med. 2017;163(4):523-527.
Taghizadeh RR, Cetrulo KJ, Cetrulo CL. Wharton’s Jelly stem cells: future clinical applications. Placenta. 2011;32(Suppl 4):S311-S315.
Ullah I, Subbarao RB, Rho GJ. Human mesenchymal stem cells — current trends and future prospective. Biosci. Rep. 2015;35(2). pii: e00191. doi: https://doi.org/10.1042/BSR20150025.
Zhang B, Shen L, Shi H, Pan Z, Wu L, Yan Y, Zhang X, Mao F, Qian H, Xu W. Exosomes from human umbilical cord mesenchymal stem cells: identification, purification, and biological characteristics. Stem Cells Int. 2016;2016. ID 1929536. doi: https://doi.org/10.1155/2016/1929536.
Zhang B, Wu X, Zhang X, Sun Y, Yan Y, Shi H, Zhu Y, Wu L, Pan Z, Zhu W, Qian H, Xu W. Human umbilical cord mesenchymal stem cell exosomes enhance angiogenesis through the Wnt4/b-catenin pathway. Stem Cells Transl. Med. 2015;4(5):513-522. doi: https://doi.org/10.5966/sctm.2014-0267.
Zhao Y, Sun X, Cao W, Ma J, Sun L, Qian H, Zhu W, Xu W. Exosomes derived from human umbilical cord mesenchymal stem cells relieve acute myocardial ischemic injury. Stem Cells Int. 2015;2015. ID 761643. doi: https://doi.org/10.1155/2015/761643.
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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 3, pp. 146-151, September, 2018
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Romanov, Y.A., Volgina, N.E., Dugina, T.N. et al. Human Umbilical Cord Mesenchymal Stromal Cell-Derived Microvesicles Express Surface Markers Identical to the Phenotype of Parental Cells. Bull Exp Biol Med 166, 124–129 (2018). https://doi.org/10.1007/s10517-018-4300-x
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DOI: https://doi.org/10.1007/s10517-018-4300-x