Protein G is present in group G streptococcus strain (G4223); the IgG-binding part of this protein contains three IgG-binding domains and binds human IgG with very high activity. We obtained two recombinant polypeptides G4223 and G14223 with high IgG-binding activity. Polypeptide G14223 consisting of three IgG-binding domains and W region has higher molecular weight and is characterized by higher affinity for IgG than polypeptide G4223 consisting of only three IgG-binding domains. It was shown that polypeptide affinity depends on its structure and size.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 165, No. 3, pp. 349-354, March, 2018
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Bormotova, E.A., Gupalova, T.V. Expression of New Recombinant IgG-Binding Polypeptides and Analysis of Their Capacity to Bind Human IgG. Bull Exp Biol Med 165, 373–377 (2018). https://doi.org/10.1007/s10517-018-4173-z
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DOI: https://doi.org/10.1007/s10517-018-4173-z