We studied peculiarities of the structure of human entorhinal cortex at weeks 20-26 of gestation (10 hemispheres). The samples were Nissl-stained and immunohistochemically treated with antibodies to parvalbumin, calretinin, calbindin, and cytoskeleton proteins (MAP2 and N200). 3D-reconstruction of the entorhinal cortex from serial sections was performed, caudomedial and rostrolateral areas were isolated. Parvalbumin+ cells in layer I, discrete distribution of layer II cells with colocalization of MAP2 and calretinin at the border with layer I, and two sublayers Va and Vb with MAP2+ neurons were typical for the caudomedial area. Rostrolateral area was characterized by the homogenous layer II with big amount of cells, high density of MAP2+ neurons only in layer III, and the unique layer V. Reelin+ Cajal—Retzius cells and N200+ fiber plexus in layer I were observed in the caudomedial and rostrolateral areas of the entorhial cortex. Layer IV was represented by a cell-free desiccant.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 164, No. 10, pp. 500-505, October, 2017
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Zykin, P.A., Moiseenko, I.A., Tkachenko, L.A. et al. Peculiarities of Cyto- and Chemoarchitectonics of Human Entorhinal Cortex during the Fetal Period. Bull Exp Biol Med 164, 497–501 (2018). https://doi.org/10.1007/s10517-018-4020-2
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DOI: https://doi.org/10.1007/s10517-018-4020-2