We studied the effect of IFNα-2b and IFNβ-1a on phasic and tonic contractions of isolated bovine mesenteric lymphatic vessels and nodes. IFNα-2b and IFNβ-1a in concentrations of 250-1000 U/ml produced dose-dependent negative chronotropic and inotropic effects on spontaneous phasic contractions and tonus of lymphatic vessels and nodes. In de-endothelialized lymphatic vessels and nodes, IFNα-2b and IFNβ-1a in the same concentrations had less pronounced inhibitory effect on spontaneous contraction and tonus. L-NAME (100 μM) and charybdotoxin (0.1 μM with 0.5 μM apamine) significantly attenuated the inhibitory effect of IFNα-2b on phasic and tonic contractions of lymph nodes. L-NAME (100 μM) and indomethacin (10 μM) significantly reduced the IFNα-2b-induced inhibitory effect on phasic and tonic contractions of lymph node. These results indicate that IFNα-2b and IFNβ-1a have a pronounced inhibitory effect on the phasic and tonic contractions of bovine mesenteric lymphatic vessels and nodes. The responses are endothelium-dependent and are determined by production of NO and endothelium-dependent hyperpolarizing factor by endotheliocytes in lymphatic vessels and by production of NO and prostacyclin by endotheliocytes in the lymphatic nodes.
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Lobov GI, Pan’kova MN. Effect of histamine on contractile activity of smooth muscles in bovine mesenteric lymph nodes. Bull. Exp. Biol. Med. 2012;152(4):406-408.
Lobov GI, Pan’kova MN. Lymph transport in lymphatic nodes: mechanisms of regulation. Ross. Fiziol. Zh. 2012;98(11):1350-1361. Russian.
Bosinger SE, Utay NS. Type I interferon: understanding its role in HIV pathogenesis and therapy. Curr. HIV/AIDS Rep. 2015;12(1):41-53.
Campbell WB, Gauthier KM. What is new in endotheliumderived hyperpolarizing factors? Curr. Opin. Nephrol. Hypertens. 2002;11(2):177-183.
Dong Y, Watabe H, Cui J, Abe S, Sato N, Ishikawa H, Yoshitomi T. Reduced effects of endothelium-derived hyperpolarizing factor in ocular ciliary arteries from spontaneous hypertensive rats. Exp. Eye Res. 2010;90(2):324-329.
Fabrizi F, Dixit V, Messa P, Martin P. Pegylated Interferon mono-therapy of chronic hepatitis C in the dialysis population: systematic review and meta-analysis. Ther. Apher. Dial. 2015;19(6):611-621.
Frömel T, Fleming I. Whatever happened to the epoxyeicosatrienoic Acid-like endothelium-derived hyperpolarizing factor? The identification of novel classes of lipid mediators and their role in vascular homeostasis. Antioxid. Redox Signal. 2015;22(14):1273-1292.
Jin X, Satoh-Otonashi Y, Zamami Y, Takatori S, Hashikawa-Hobara N, Kitamura Y, Kawasaki H. New molecular mechanisms for cardiovascular disease: contribution of endotheliumderived hyperpolarizing factor in the regulation of vasoconstriction in peripheral resistance arteries. J. Pharmacol. Sci. 2011;116(4):332-336.
Luksha L, Agewall S, Kublickiene K. Endothelium-derived hyperpolarizing factor in vascular physiology and cardiovascular disease. Atherosclerosis. 2009;202(2):330-344.
Martinez-León JB, Segarra G, Medina P, Vila JM, Lluch P, Peiró M, Otero E, Lluch S. Ca2+-activated K+ channels mediate relaxation of forearm veins in chronic renal failure. J. Hypertens. 2003;21(10):1927-1934.
Sano E, Tashiro S, Tsumoto K, Ueda T. Differential effects of IFN-β on the survival and growth of human vascular smooth muscle and endothelial cells. Biores. Open Access. 2015;4(1):1-15.
Yeh ML, Peng CY, Dai CY, Lai HC, Huang CF, Hsieh MY, Huang JF, Chen SC, Lin ZY, Yu ML, Chuang WL. Pegylatedinterferon alpha therapy for treatment-experienced chronic hepatitis B patients. PLoS One. 2015;10(4):e0122259. doi: https://doi.org/10.1371/journal.pone.0122259.
Zawieja DC. Contractile physiology of lymphatics. Lymphat. Res. Biol. 2009;7(2):87-96.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 164, No. 8, pp. 145-149, August, 2017
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Unt, D.V., Lobov, G.I. Inhibitory Effect of Interferons on Contractive Activity of Bovine Mesenteric Lymphatic Vessels and Nodes. Bull Exp Biol Med 164, 123–126 (2017). https://doi.org/10.1007/s10517-017-3939-z
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DOI: https://doi.org/10.1007/s10517-017-3939-z