Expression of choline acetyltransferase, 200-kDa neurofilament protein, 28-kDa calbindin, neuronal NO synthase, caspase 3, and Ki-67 in the motor neurons of spinal cord segments T3-T5 in male C57Bl/6 mice after 30-day space flight in the Bion-M1 biosatellite was studied by immunohistochemical methods. Under conditions space flight, the size of motoneurons increased, the number of neurons containing choline acetyltransferase and neurofilaments, decreased, and the number of calbindin-positive neurons increased; motoneurons, expressing neuronal NO synthase and caspase 3 appeared, while Ki-67 was not detected. Fragmentation of neurons with the formation structures similar to apoptotic (residual) bodies was observed in individual caspase 3-positive motoneurons.
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Andreev-Andrievsky AA, Shenkman BS, Popova AS, Dolguikh ON, Anokhin KV, Soldatov PE, Ilyin EA, Sychev VN. Experimental studies with mice on the program of the biosatellite BION-M1 mission. Aviakosm. Ekol. Med. 2014;48(1):14-27. Russian.
Islamov RR, Valiullin VV, Nikolsky EE, Tyapkina OV, Bukharaeva EA, Yagodina LO, Ibragimova NN, Kozlovskaya IB. Expression of choline acetyltransferase in rat spinal motoneurons after antiorthostatic suspemsion. Doklady Biol. Sci. 2007;414(1):205-207.
Krasnov IB. Deafferentation and commutation change of brain structures in weightlessness. Aviakosm. Ekol. Med. 2013;47(4):83-84. Russian.
Polyakov IV, Louri O, Edzherton VP, Krasnov IB. Histochemistry and morphology of the anterior horns of the spinal cord in rats after 9-day spaceflight Aviakosm. Ekol. Med. 1995;29(1):30-32. Russian.
Porseva VV, Shilkin VV, Strelkov AA, Masliukov PM. Calbindin-containing neurons of the ventral horn of murine spinal cord gray matter. Morfologiya. 2014;146(4):21-25. Russian.
Porseva VV, Shilkin VV, Strelkov AA, Masliukov PM. Subpopulation of calbindin-immunoreactive innrerneurons in toe dorsal horn of toe mice spinal cord. Tsitologiya. 2014;56(8):612-618. Russian.
Fuchs A, Lirk P, Stucky C, Abram SE, Hogan QH. Painful nerve injury decreases resting cytosolic calcium concentrations in sensory neurons of rats. Anesthesiology. 2005;102(6):1217-1225.
Gibbons SJ, Brorson JR, Bleakman D, Chard PS, Miller RJ. Calcium influx and neurodegeneration. Ann. N.Y. Acad. Sci. 1993;679:22-33.
Ishihara A, Ohira Y, Roy RR, Nagaoka S, Sekiguchi C, Hinds WE, Edgerton VR. Comparison of the response of motoneurons innervating perineal and hind limb muscles to spaceflight and recovery. Muscle Nerv. 2000;23(5):753-762.
Kim KH, Kim JI, Han JA, Choe MA, Ahn JH. Upregulation of neuronal nitric oxide synthase in the periphery promotes pain hypersensitivity after peripheral nerve injury. Neuroscience. 2011;190:367-378.
Kozlovskaya IB, Sayenko IV, Sayenko DG, Miller TF, Khusnutdinova DR, Melnik KA. Role of support afferentation in control of the tonic muscle activity. Acta Astronaut. 2007;60(4-7):285-294.
Krasnov IB. Gravitational neuromorphology. Adv. Space Biol. Med. 1994;4:85-110.
Masliukov PM, Emanuilov AI, Madalieva LV, Moiseev KY, Bulibin AV, Korzina MB, Porseva VV, Korobkin AA, Smirnova VP. Development of nNOS-positive neurons in the rat sensory and sympathetic ganglia. Neuroscience. 2014;256:271-281.
Perečko T, Drábiková K, Nosáľ R, Harmatha J, Jančinová V. Involvement of caspase 3 in stilbene derivatives induced apoptosis of human neutrophils in vitro. Interdiscip. Toxicol. 2012;5(2):76-80.
Schwaller B. The use of transgenic mouse models to reveal the functions of Ca2+ buffer proteins in excitable cells. Biochim. Biophys. Acta. 2012;1820(8):1294-1303.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 162, No. 9, pp. 318-321, September, 2016
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Porseva, V.V., Shilkin, V.V., Strelkov, A.A. et al. Changes in the Neurochemical Composition of Motor Neurons of the Spinal Cord in Mice under Conditions of Space Flight. Bull Exp Biol Med 162, 336–339 (2017). https://doi.org/10.1007/s10517-017-3609-1
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DOI: https://doi.org/10.1007/s10517-017-3609-1