Platelet lysate prepared from donor platelet concentrate and pooled according to a developed technique stimulates migration of multipotent mesenchymal stromal cells of the human adipose tissue and promotes healing of the monolayer defect in cultures of human fibroblasts and multipotent mesenchymal stromal cells in vitro in concentrations close those of fetal calf serum (5-10%). Lysate of platelets from platelet-rich rat blood plasma stimulated healing of the skin defect by promoting epithelialization and granulation tissue formation. The regenerative properties of platelet lysate in vivo increased with increasing its concentration.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Zhiburt EB, Madzaev SR. Platelet Harvesting and Transfusion. Moscow, 2013. Russian.
Kirilova LA, Fomichev NG, Podorozhnaya VT, Etitein YuM. Combinative use of osteoplasty and platelet-enriched plasma in traumatology and orthopedics. Travmatologiya Ortopediya Rossii. 2008;(3):63-67. Russian.
Sergeeva NS, Sviridova IK, Kirsanova VA, Marshutina NV, Akhmedova SA, Makhmurova NT, Titova NS. Culturing and characterization of postnatal non-hemopoietic stem cells from human adipose tissue. Mol. Med. 2006;(2):23-29. Russian.
Sergeeva NS, Shanskii YaD, Sviridova IK, Kirsanova VA, Akhmedova SA, Kuvshinova EA, Meisner IS. Biological effects of platelet lysate added to the culture medium on human cells. Geny Kletki. 2014;9(1):77-85. Russian.
Sergeeva NS, Shanskii YaD, Sviridova IK, Kirsanova VA, Akhmedova SA, Kaprin AD, Pirogov SA. A method of preparation of platelet lysate-based growth supplement from donor platelet concentrate for the use as medium additive during expansion of stem, progenitor, and differentiated tumor cells. Patent Application No. 2016115622. Bull. No. 25. Published September 10, 2016. Russian.
Shanskii YD, Sergeeva NS, Sviridova IK, Kirakozov MS, Kirsanova VA, Akhmedova SA, Antokhin AI, Chissov VI. Human platelet lysate as a promising growth-stimulating additive for culturing of stem cells and other cell types. Bull. Exp. Biol. Med. 2013;156(1):146-151.
Ben Azouna N, Jenhani F, Regaya Z, Berraeis L, Ben Othman T, Ducrocq E, Domenech J. Phenotypical and functional characteristics of mesenchymal stem cells from bone marrow: comparison of culture using different media supplemented with human platelet lysate or fetal bovine serum. Stem Cell Res. Ther. 2012;3(1):6. doi: 10.1186/scrt97.
Bieback K. Platelet lysate as replacement for fetal bovine serum in mesenchymal stromal cell cultures. Transfus. Med. Hemother. 2013;40(5):326-335.
Capelli C, Domenghini M, Borleri G, Bellavita P, Poma R, Carobbio A, Micò C, Rambaldi A, Golay J, Introna M. Human platelet lysate allows expansion and clinical grade production of mesenchymal stromal cells from small samples of bone marrow aspirates or marrow filter washouts. Bone Marrow Transplant. 2007;40(8):785-791.
Chandra RK, Handorf C, West M, Kruger EA, Jackson S. Histologic effects of autologous platelet gel in skin flap healing. Arch. Facial Plast. Surg. 2007;9(4):260-263.
DeRossi R, Coelho AC, Mello GS, Frazílio FO, Leal CR, Facco GG, Brum KB. Effects of platelet-rich plasma gel on skin healing in surgical wound in horses. Acta Cir. Bras. 2009;24(4):276-281.
Fréchette JP, Martineau I, Gagnon G. Platelet-rich plasmas: growth factor content and roles in wound healing. J. Dent. Res. 2005;84(5):434-439.
Kondo T, Ishida Y. Molecular pathology of wound healing. Forensic Sci. Int. 2010;203(1-3):93-98.
Mojica-Henshaw MP, Jacobson P, Morris J, Kelley L, Pierce J, Boyer M, Reems JA. Serum-converted platelet lysate can substitute for fetal bovine serum in human mesenchymal stromal cell cultures. Cytotherapy. 2013;15(12):1458-1468.
Mossman T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods. 1983;65(1-2):55-63.
Nakamura S, Ishihara M, Takikawa M. Platelet-rich plasma (PRP) promotes survival of fat-grafts in rats. Ann. Plast. Surg. 2010;65):101-106.
Rozman P, Bolta Z. Use of platelet growth factors in treating wounds and soft-tissue injuries. Acta Dermatovenerol. Alp. Pannonica Adriat. 2007;16(4):156-165.
Smith SE, Roukis TS. Bone and wound healing augmentation with platelet-rich plasma. Clin. Podiatr. Med. Surg. 2009;26(4):559-588.
Suzuki S, Ikada Y. Biomaterials for Surgical Operation. N.Y, 2012.
Thiriet M. Growth Factors. Control of Cell Fate in the Circulatory and Ventilatory Systems. Berlin, 2012. P. 177-277.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 3, pp. 172-179, July, 2016
Rights and permissions
About this article
Cite this article
Sergeeva, N.S., Shanskii, Y.D., Sviridova, I.K. et al. Analysis of Reparative Activity of Platelet Lysate: Effect on Cell Monolayer Recovery In Vitro and Skin Wound Healing In Vivo . Bull Exp Biol Med 162, 138–145 (2016). https://doi.org/10.1007/s10517-016-3563-3
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-016-3563-3