Cell cultures isolated from human endometrium by enzyme digestion consisted of highly viable fibroblast-like mesenchymal cells expressing CD90, CD73, and CD105. During passage 1, the cultures contained a small fraction of cytokeratin-7+ epithelial cells that disappeared by passage 2. The cultures from the endometrium could be induced to adipogenic, osteogenic and chondrogenic differentiation in vitro. These findings suggest that human endometrium is a convenient source of biomaterial for minimally invasive isolation of cultures that exhibit typical morphology and immunophenotypic profile of resident multipotent mesenchymal stromal cells retain high viability in vitro.
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References
S. V. Anisimov, V. I. Zemel’ko, T. M. Grinchuk, and N. N. Nikol’skii, Tsitologiya, 55, No. 1, 5-10 (2013).
V. N. Veryasov, A. M. Savilova, O. A. Buyanovskaya, et al., Bull. Exp. Biol. Med., 157, No. 1, 119-124 (2014).
A. P. Domnina, I. I. Fridlyanskaya, V. I. Zemel’ko, et al., Tsitologiya, 55, No. 1, 69-74 (2013).
J. G. Allickson, A. Sanchez, N. Yefi menko, et al., Open Stem Cell J., 3, 4-10 (2011).
N. H. Cho, Y. K. Park, Y. T. Kim, et al., Fertil. Steril., 81, No. 2, 403-407 (2004).
M. Dominici, K. Le Blanc, I. Mueller, et al., Cytotherapy, 8, No. 4, 315-317 (2006).
K. M. French and M. E. Davis, Methods Mol. Biol., 1181, 39- 50 (2014).
C. E. Gargett and H. Masuda, Mol. Hum. Reprod., 16, No. 11, 818-834 (2010).
C. E. Gargett, K. E. Schwab, R. M. Zillwood, et al., Biol. Reprod., 80, No. 6, 1136-1145 (2009).
J. G. Hu and R. Yuan, Med. Sci. Monit., 17, No. 4, BR103-BR109 (2011).
A. Pacchiarotti, D. Caserta, M. Sbracia, and M. Moscarini, Fertil. Steril., 95, No. 3, 1171-1173 (2011).
E. B. Rangel, S.A. Gomes, R.A. Dulce, et al., Stem Cells, 31, No. 8, 1644-1656 (2013).
A. N. Schüring, N. Schulte, R. Kelsch, et al., Fertil. Steril., 95, No. 1, 423-426 (2011).
S. Taghavi, T. E. Sharp 3rd, J. M. Duran, et al., Clin. Transl. Sci., doi: 10.1111/cts.12251 (2015).
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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 252-257, October, 2015
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Savilova, A.M., Yushina, M.N., Rudimova, Y.V. et al. Characteristics of Multipotent Mesenchymal Stromal Cells Isolated from Human Endometrium. Bull Exp Biol Med 160, 560–564 (2016). https://doi.org/10.1007/s10517-016-3218-4
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DOI: https://doi.org/10.1007/s10517-016-3218-4