Abstract
The lipid metabolomic profile has been well defined in the pathogenesis and differential diagnosis in patients with different myeloid diseases. We assumed that the serum lipid metabolites could also help the diagnosis and prognostic prediction of aplastic anemia (AA). In this study, serum lipid profiles were explored in AA patients before and after cyclosporin (CsA) treatment. Meanwhile, hypocellular myelodysplastic syndrome (h-MDS) patients and the healthy volunteers were compared as controls. 15 AA patients, 11 h-MDS patients and 20 age and sex matched health controls were enrolled. All the AA patients were diagnosed to be non-severe aplastic anemia with transfusion dependency and were treated by CsA 3–5 mg/kg/d for at least 6 months. AA patients had decreased arachidonic acid pathway metabolites and retinol metabolism-related metabolites as compared with h-MDS and the health (P < 0.05), whereas h-MDS patients had increased metabolism of proline and threonine and abnormal sphingolipid metabolism compared with AA patients and the normal controls. After 6 month of CsA treatment, serum arachidonic acid, PGE2, PGJ2, 15(S)-HETE, leukotriene B4 and Protectin D1 decreased significantly. Patients who had response to CsA had higher levels of baseline protectin D1 (P = 0.011), leukotriene B4 (P = 0.011), 15(S)-HETE (P = 0.004) and all-trans-retinal (P = 0.000) than those who had no response.
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Funding
This study was supported by grants from Beijing Natural Science Foundation (7192168), the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences (2019XK 320047), CAMS Innovation Fund for Medical Sciences (2021-I2M-1-003), the National Key Research and Development Program of China (2016YFC0901500) and National Natural Science Foundation of China (81970106).
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All authors contributed to the study conception and design. MC, YD and CY helped to collect clinical data and provide critical suggestions. Material preparation and data analysis were performed by JR and BH The first draft of the manuscript was written by JR, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Ruan, J., Yang, C., Du, Y. et al. Plasma lipidome acts as diagnostic marker and predictor for cyclosporin response in patients with aplastic anemia. Clin Exp Med 23, 767–776 (2023). https://doi.org/10.1007/s10238-022-00826-z
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DOI: https://doi.org/10.1007/s10238-022-00826-z