Abstract
In this study, bacteria associated with marine organisms were screened for the production of exopolysaccharides (EPSs) on MY media containing sea salts (2.5%–10%). Three selected isolates were identified as Alteromonas sp. PRIM-21, Nitratireductor sp. PRIM-24 and Enterobacter sp. PRIM-26 using 16S rRNA gene sequencing. Optimization of the growth and EPS production kinetics in relation to incubation time were assessed. The purified EPS yield was 590, 650 and 540 mg·L−1 culture media respectively in Alteromonas sp. PRIM-21, Nitratireductor sp. PRIM-24 and Enterobacter sp. PRIM-26. Biochemical and FTIR analyses revealed the presence of biologically important functional groups in the EPS produced by all the three isolates. The EPS produced by Nitratireductor sp. PRIM-24 and Alteromonas sp. PRIM-21 showed 2.0% sulfate content. These bacterial EPS also showed antioxidant and emulsifying activities and the EPS produced by Enterobacter sp.PRIM-26 showed significantly higher antioxidant activities in terms of superoxide (IC50 0.33 mg·mL−1) and DPPH (IC50 0.44 mg·mL−1) radical scavenging. It also showed higher emulsifying activities against selected hydrophobic substrates with EI24 values above 60%. From the results of the study, it can be concluded that the isolated bacteria produce EPS that can be investigated in detail for biotechnological applications.
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This work was financially supported by the India and author Priyanka P acknowledges the Yenepoya University Junior research fellowship. The authors thank BRNS (2013/34/19/BRNS/1210).
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Priyanka, P., Arun, A.B., Young, C.C. et al. Prospecting exopolysaccharides produced by selected bacteria associated with marine organisms for biotechnological applications. Chin J Polym Sci 33, 236–244 (2015). https://doi.org/10.1007/s10118-015-1581-7
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DOI: https://doi.org/10.1007/s10118-015-1581-7