Abstract
Dietary exposure to advanced glycation end products (AGEs) formed from proteins and reducing sugars is of increasing concern to human health. AGEs may form in protein-based powders containing sugars for instant beverages during drying and storage of the product. Chlorogenic acid, a plant phenol characteristic of coffee, was found to protect against the formation of AGEs at a concentration of 50mM during heating of β-lactoglobulin in the presence of glucose as a reducing sugar in 30% aqueous ethanol at 70°C. Epicatechin, a plant phenol characteristic of green tea, had no similar effect for the equivalent concentration of phenol on the formation of AGEs. Immunochemical detection (ELISA) using polyclonal antibodies raised against AGEs showed a dose-dependent effect of protection by chlorogenic acid on AGE formation and is recommended for routine quality control of sugar containing milk-based powders for instant beverages.
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References
Poulsen MW, Hedegaard RV, Andersen JM, de Courten B, Bügel S, Nielsen J, Skibsted LH, Dragsted LO. Advanced glycation endproducts in food and their effects on health. Food Chem. Toxicol. 60: 10–37 (2013)
Jang DS, Lee GY, Lee YM, Kim YS, Sun H, Kim DH, Kim JS. Flavan-3-ols having a gamma-lactam from the roots of actinidia arguta inhibit the formation of advanced glycation end products in vitro. Chem. Pharm. Bull. 57: 397–400 (2009)
Tsuji-Naito K, Saeki H, Hamano M. Inhibitory effects of Chrysanthemum species extracts on formation of advanced glycation end products. Food Chem. 116: 854–859 (2009)
Yin J, Hedegaard RV, Skibsted L H, Andersen M L. Epicatechin and epigallocatechin gallate inhibit formation of intermediary radicals during heating of lysine and glucose. Food Chem. 146: 48–55 (2013)
Reddy S, Bichler J, Wellsknecht KJ, Thorpe SR, Baynes JW. N-Epsilon-(carboxymethyl)lysine is a dominant advanced glycation end-product (AGE) antigen in tissue proteins. Biochemistry-US 34: 10872–10878 (1995)
Liu L, Hedegaard RV, Skibsted LH. Formation of advanced glycation end products (AGEs) are influenced by lipids in milk powders. Aust. J. Chem. 66: 1074–1079 (2013)
Yin J, Andersen ML, Skibsted LH. Reduction of ferrylmyoglobin by theanine and green tea catechins. Importance of specific acid catalysis. J. Agr. Food Chem. 61: 3159–3165 (2013)
Hedegaard RV, Liu L, Skibsted LH. Quantification of radicals formed heating ß-lactoglobulin with glucose in aqueous ethanol. Food Chem. 167: 185–190 (2014)
Vlassara H, Stricker GE. AGE restriction in diabetes mellitus: A paradigm shift. Nat. Rev. Endocrinol. 7: 526–538 (2011)
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Liu, L., Hedegaard, R.V. & Skibsted, L.H. Effect of plant polyphenols on the formation of advanced glycation end products from β-lactoglobulin. Food Sci Biotechnol 26, 389–391 (2017). https://doi.org/10.1007/s10068-017-0053-y
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DOI: https://doi.org/10.1007/s10068-017-0053-y