Abstract
Cross-linking of FcεRI with multivalent allergen and specific IgE on mast cells and initiates activation of signal cascades that lead to IgE-mediated allergic responses. Kaempferol is a phenolic compound contained in natural substances including the stamens of flavonoid-rich Nelumbo nucifera stamens. In this study, the effects of kaempferol on allergic reaction induced by an FcεRI α chain antibody were investigated. Western blot analysis revealed that the protein expression of FcεRI and the phosphorylation of Syk, Lyn, and extracellular signal-regulated kinases (ERK) 1/2 were dose-dependently inhibited by kaempferol. β-Hexosaminidase was reduced to 97.9, 89.1, 75.1, and 55.8% when treated with 5, 10, 20, and 40 μM kaempferol, respectively. From the results, the negative regulation of basophil activation with kaempferol via the suppression of FcεRI expression and degranulation may be suggested to be through inhibition of Lyn and Syk, and ERK 1/2 activation.
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Mukherjee PK, Balasubramanian R, Saha K, Saha BP, Pal M. A review on Nelumbo nucifera Gaertn. Anc. Sci. Life 15: 268–276 (1996)
Mukherjee PK, Saha K, Balasubramanian R, Pal M, Saha BP. Studies on psychopharmacological effects of Nelumbo nucifera Gaertn. rhizome extract. J. Ethnopharmacol. 54: 63–67 (1996)
Mukherjee PK, Saha K, Pal M, Saha BP. Effect of Nelumbo nucifera rhizome extract on blood sugar level in rats. J. Ethnopharmacol. 58: 207–213 (1997)
Van Bergan PF, Hatcher PG, Boon JJ, Collinson ME, De Leeuw JW. Macromolecular composition of the propagule wall of Nelumbo nucifera. Phytochemistry 45: 601–610 (1997)
Sohn DH, Kim YC, Oh SH, Park EJ, Li X, Lee BH. Hepatoprotective and free radical scanvenging effects of Nelumbo nucifera. Phytomedicine 10: 165–169 (2003)
Jiang Y, Ng TB, Liu Z, Wang C, Li N, Qiao W, Liua F. Immunoregulatory and anti-HIV-1 enzyme activities of antioxidant components from lotus (Nelumbo nucifera Gaertn.) rhizome. Bioscience Rep. 31: 381–390 (2011)
Jung HA, Kim JE, Chung HY, Choi JS. Antioxidant principles of Nelumbo nucifera stamens. Arch. Pharm. Res. 26: 279–285 (2003)
Lin HY, Kuo YH, Lin YL, Chiang W. Antioxidant effect and active components from leaves of lotus (Nelumbo nucifera). J. Agr. Food Chem. 57: 6623–6629 (2009)
La Cour B, Molgaard P, Yi Z. Traditional chinese medicine in treatment of hyperlipidaemia. J. Ethnopharmacol. 46: 125–129 (1995)
Goo HR, Choi JS, Na DH. Simultaneous determination of quercetin and its glycosides from the leaves of Nelumbo nucifera by reversedphase high-performance liquid chromatography. Arch. Pharm. Res. 32: 201–206 (2009)
Kim HK, Park HR, Lee JS, Chung TS, Chung HY, Chung J. Downregulation of iNOS and TNF-α expression by kaempferol via NF-κB inactivation in aged rat gingival tissues. Biogerontology 8: 399–408 (2007)
Mukherjee D, Biswas A, Bhadra S, Pichairajan V, Biswas T, Saha BP, Mukherjee PK. Exploring the potential of Nelumbo nucifera rhizome on membrane stabilization, mast cell protection, nitric oxide synthesis, and expression of costimulatory molecules. Immunopharm. Immunot. 32: 466–472 (2010)
Lim SS, Jung YJ, Hyun SK, Lee YS, Choi JS. Rat lens aldose reductase inhibitory constituents of Nelumbo nucifera stamens. Phytother. Res. 20: 825–830 (2006)
Shim SY, Choi JS, Byun DS. Kaempferol isolated from Nelumbo nucifera stamens negatively regulates FcεRI expression in human basophilic KU812F cells. J. Microbiol. Biotechn. 19: 155–160 (2009)
Nguyen TTT, Tran E, Ong CK, Lee SK, Do PT, Huynh TT, Nguyen TH, Lee JJ, Tan Y, Ong CS, Huynh H. Kaempferol-induced growth inhibition and apoptosis in A549 lung cancer cells is mediated by activation of MEK-MAPK. J. Cell. Physiol. 197: 110–121 (2003)
Beaven MA, Metzger H. Signal transduction Fc receptors; The Fc epsilon RI case. Immunol. Today 14: 222–226 (1993)
Metzer H. The high affinity receptor for IgE on mast cells. Clin. Exp. Immunol. 21: 269–279 (1991)
Kinet JP, Blank U, Brini A, Jouvin MH, Kuster H, Mejan O, Ra C. The high affinity receptor for immunoglobulin E: A target for therapy of allergic diseases. Int. Arch. Allergy Imm. 94: 51–55 (1991)
Kinet JP. The high-affinity IgE receptor (FcεRI): From physiology to pathology. Annu. Rev. Immunol. 17: 931–972 (1999)
Chen CC, Chow MP, Huang WC, Lin YC, Chang YJ. Flavonoids inhibit tumor necrosis factor-a-induced up regulation of intercellular adhesion molecule-1 (ICAM-1) in respiratory epithelial cells through activator protein-1 and nuclear factor-κB; Structure-activity relationships. Mol. Pharmarcol. 66: 683–693 (2004)
Hakimi JC, Seals JA, Kondas L, Pettine W, Danko W, Kochan J. The α subunit of the human IgE receptor (FcεRI) is sufficient for high affinity IgE binding. J. Biol. Chem. 265: 22079–22081 (1990)
Siraganian RP. Mast cell signal transduction from the high-affinity IgE receptor. Curr. Opin. Immunol. 15: 639–646 (2003)
Siraganian RP, Rodrigo OC, Emilia AB, Juan Z. Mast cell signaling: The role of protein tyrosine kinase Syk, its activation and screening methods for new pathway participants. FEBS Lett. 584: 4933–4940 (2010)
Rivera J. Molecular adapters in FcεRI signaling and the allergic response. Curr. Opin. Immunol. 14: 688–693 (2002)
Poornima P, Weng CF, Padma VV. Neferine, an alkaloid from lotus seed embryo, inhibits human lung cancer cell growth by MAPK activation and cell cycle arrest. Biofactors 40: 121–131 (2014)
Zhang X, Liu Z, Xu B, Sun Z, Gong Y, Shao C. Neferine, an alkaloid ingredient in lotus seed embryo inhibits proliferation of human osteosarcoma cells by promoting p38 MAPK-mediated p21 stabilization. Eur. J. Pharmacol. 677: 47–54 (2012)
Fujimura Y, Tachibana H, Yamada K. A tea catechin suppresses the expression of the high-affinity IgE receptor FcεRI in human basophilic KU812 cells. J. Agr. Food Chem. 49: 2527–2531 (2001)
Fujimura Y, Tachibana H, Maeda-Yamamoto M, Miyase T, Sano M, Yamada K. Antiallergic tea catechin, (-)-epigallocatechin-3-O-(3-O-methyl)-gallate, suppresses FcεRI expression in human basophilic KU812 cells. J. Agr. Food Chem. 50: 5729–5734 (2002)
Fujimura Y, Tachibana H, Yamada K. Lipid raft-associated catechin suppresses the FcεRI expression by inhibiting phosphorylation of the extracellular signal-regulated kinase 1/2. FEBS Lett. 556: 204–210 (2004)
Shim SY, Sun HJ, Song YH, Kim HR, Byun DS. Inhibitory effects of blueberry root methanolic extract on degranulation in KU812F cells. Food Sci. Biotechnol. 19: 1185–1189 (2010)
Malich G, Markovic B, Winder C. The sensitivity and specificity of the MTS tetrazolium assay for detecting the in vitro cytotoxicity of 20 chemicals using human cell lines. Toxicology 124: 179–192 (1997)
Suzuki Y, Inoue T, Ra C. Calcium signaling in mast cells: Focusing on L-type calcium channels. Adv. Exp. Med. Biol. 740: 955–977 (2012)
Kalensnikoff J, Huber M, Lam V, Damen JE, Zhang J, Siraganian RP, Krystal G. Monomeric IgE stimulates signaling pathway in mast cells that lead to cytokine production and cell survival. Immunity 14: 801–811 (2001)
Miura K, Lavens-Phillips S, MacGlashan DW. Localizing a control region in the pathway to leukotriene C4 secretion following stimulation of human basophils with anti-IgE antibody. J. Immunol. 167: 7027–7037 (2001)
Masuda A, Yoshikai Y, Aiba K, Matsuguchi T. Th2 cytokine production from mast cells is directly induced by lipopolysaccharide and distinctly regulated by c-jun N-terminal kinase and p38 pathways. J. Immunol. 169: 3801–3810 (2002)
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Shim, SY., Park, JR. & Byun, DS. Kaempferol isolated from Nelumbo nucifera stamens inhibits phosphorylation of ERK 1/2, Syk, and Lyn in FcεRI-mediated allergic reaction. Food Sci Biotechnol 24, 1475–1480 (2015). https://doi.org/10.1007/s10068-015-0190-0
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DOI: https://doi.org/10.1007/s10068-015-0190-0