Abstract
In this study, 17 Y-chromosomal short tandem repeats (Y-STRs) were analyzed in 1026 male individuals of Han populations in Changchun City, Jilin Province, Northeast China. The haplotype diversity is 0.99892. The Changchun Han population is close to most Han populations and different from most other minority populations of China. Additionally, the Changchun Han show more affiliations with Han populations in North and Northeast China. These data provide a reference for the Y-STR database in Jilin Province, and they may be valuable for population genetic analysis.
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According to historical records, a major part of modern Han ethnic group in northeastern China are descendants of immigrant who came from Shandong Province, Hebei Province, and adjacent region since the latter half of nineteenth century [1]. A total of 1026 male individuals of Han population were collected from Changchun City, Jilin Province in Northeast China. All individuals provided informed consent, and then, peripheral blood samples were collected. The ethics committee for biological research at the Fudan School of Life Sciences approved the study. Genomic DNA was extracted using Gentra Puregene Blood Kit (Qiagen). Polymerase chain reaction (PCR) was performed using the AmpFlSTR Yfiler™ PCR Amplification Kit (Thermo Fisher Scientific Company, Carlsbad, CA, USA) in the GeneAmp PCR System 9700 (Thermo Fisher Scientific Company) targeting 17 Y-chromosomal short tandem repeat (Y-STR). PCR products were separated by capillary electrophoresis in ABI PRISM 3130xL Genetic Analyzer (Thermo Fisher Scientific Company). The GeneMapper ID software v3.2 (Thermo Fisher Scientific Company) was used for genotype assignment. Allele and haplotype frequencies, haplotype diversity (HD), haplotype match probability (HMP), discrimination capacity (DC), and Rst values were calculated using the software package Arlequin version 3.5 [2], and provided in Supplementary Tables S1, S2, S3, and S4. Results of Rst were visualized using multidimensional scaling plot (MDS) with SPSS v22 (Chicago, IL, USA). We also conducted a separated run with only Han populations. Our data has been submitted to YHRD and received the accession number Changchun, China [Han], n = 1026, YA004260.
A total of 767 different haplotypes were observed among the individuals in the Han population of the present study, of which 634 were unique and 133 were shared by 2 to 12 individuals (Table S1). The HD of Han population in this study is 0.999104. The HMP value is 0.001869, and the DC value is 0.747563. The HD, HMP, and DC of other involved 29 populations can be found in Table S2. We compared the data in this study (Han_Jilin_WQ) and an independent dataset of 196 sample of Han population (Han_Jilin_YH) in the same location from Han et al. [3]. The HD of two datasets is close, while the DC of this study is lower than that from Han et al. [3], possibly due to some unnoticed close relatives in our large dataset.
To investigate the genetic relationship between Changchun Han population and other populations in China, data of 9066 reference samples from 16 Han and 13 minority populations were obtained from earlier reports (see details in Table S2) [3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21]. As shown in Table S3 and Fig. S1, Changchun Han and most other Han populations tend to cluster together in the center of MDS plot while minority populations scatter in the periphery of MDS plot. This result is consistent with previously reported genetic homogeneity of Han populations [22], with the Han population from Luzhou City [9] in southwest of China as an exception in our analysis. When only Han populations were included in the MDS analysis, Changchun Han population showed great affiliation with Han populations from North and Northeast China (Table S4 and Fig. S2). Furthermore, our sample (Han_Jilin_WQ) is close to the dataset from the same location (Han_Jilin_YH) from Han et al. [3] in MDS plot (Fig. S2). Our analysis results are consistent with a recent study [3] and historical records about the origin of Han populations in Northeast China.
In conclusion, we report the haplotype frequency and forensic parameters based on 17 Y-STR loci of Han populations from Changchun City, Jilin Province in Northeast China. Our analysis results indicated that Changchun Han population is close to Han populations from North and Northeast China. We believe that the data is valuable for both forensics and population genetics.
References
Gottschang TR, Lary D (2000) Swallows and settlers: the great migration from North China to Manchuria, issue 87 of Michigan monographs in Chinese studies. Center for Chinese Studies, The University of Michigan, Ann Arbor
Excoffier L, Lischer HEL (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10(3):564–567. https://doi.org/10.1111/j.1755-0998.2010.02847.x
Han Y, Li L, Liu X, Chen W, Yang S, Wei L, Xia M, Ma T, Jin L, Li S (2016) Genetic analysis of 17 Y-STR loci in Han and Korean populations from Jilin Province, Northeast China. Forensic Sci Int: Genet 22:8–10. https://doi.org/10.1016/j.fsigen.2016.01.003
Xu J, Li LM, Wei LH, Nie ZY, Yang SP, Xia MY, Ma T, Sun H, Zhao XY, Ping Y, Zhou HG, Xue FZ, Zhao ZQ, Jin L, Li SL (2016) Genetic analysis of 17 Y-STR loci in Han population from Shandong Province in East China. Forensic Sci Int: Genet 22:e15–e17. https://doi.org/10.1016/j.fsigen.2016.01.016
Li LM, Yu G, Li SL, Jin L, Yan S (2015) Genetic analysis of 17 Y-STR loci from 1019 individuals of six Han populations in East China. Forensic Sci Int: Genet 20:101–102. https://doi.org/10.1016/j.fsigen.2015.10.007
Shu LL, Li LM, Yu G, Yu BX, Liu YC, Li SL, Jin L, Yan S (2015) Genetic analysis of 17 Y-STR loci in Han, Dong, Miao and Tujia populations from Hunan province, central-southern China. Forensic Sci Int: Genet 19:250–251. https://doi.org/10.1016/j.fsigen.2015.07.007
Li LM, Ma T, Xu J, Yang YJ, Yang SP, Xia MY, Sun H, Zhao XY, Ping Y, Zhou HG, Xie JH, Zhao ZQ, Jin L, Li SL (2015) Genetic analysis of 17 Y-STR loci in Han population from Gansu province, northwestern China. Forensic Sci Int: Genet 19:134–135. https://doi.org/10.1016/j.fsigen.2015.07.008
Yang YY, Yuan WL, Guo F, Jiang XH (2014) Population data of 17 Y-STR loci in Nanyang Han population from Henan Province, Central China. Forensic Sci Int: Genet 13:145–146. https://doi.org/10.1016/j.fsigen.2014.07.013
Bing L, Liang WB, Pi JH, Zhang DM, Yong D, Luo HB, Zhang LS, Lin Z (2013) Population genetics for 17 Y-STR loci(AmpFISTR®Y-filerTM) in Luzhou Han ethnic group. Forensic Sci Int: Genet 7(2):e23–e26. https://doi.org/10.1016/j.fsigen.2012.11.010
Liu Y, Liao LC, Gu MB, Ye Y (2014) Population genetics for 17 Y-STR loci in a Chinese Han population sample from Mudanjiang city, Northeast China. Forensic Sci Int: Genet 13:e16–e17. https://doi.org/10.1016/j.fsigen.2014.05.009
Bai RF, Zhang Z, Liang QZ, Lu D, Yuan L, Yang X, Shi MS (2013) Haplotype diversity of 17 Y-STR loci in a Chinese Han population sample from Shanxi Province, Northern China. Forensic Sci Int: Genet 7(1):214–216. https://doi.org/10.1016/j.fsigen.2012.10.004
Shi MS, Liu YJ, Zhang JT, Bai RF, Lv XJ, Ma SH (2015) Analysis of 24 Y chromosomal STR haplotypes in a Chinese Han population sample from Henan Province, Central China. Forensic Sci Int: Genet 17:83–86. https://doi.org/10.1016/j.fsigen.2015.04.001
Wang D, Liu FQ, Kong LX, Yuan ZY, Chen G, Ye J (2015) Population data of 17 Y-STR haplotypes in Jining Han population from Shandong province, East China. Forensic Sci Int: Genet 19:47–49. https://doi.org/10.1016/j.fsigen.2015.05.017
Luo H, Song F, Zhang LS, Hou YP (2015) Genetic polymorphism of 23 Y-STR loci in the Zhuang minority population in Guangxi of China. Int J Legal Med 129(4):737–738. https://doi.org/10.1007/s00414-015-1175-5
Guo F (2015) Population genetics for 17 Y-STR loci in Mongolian ethnic minority from Liaoning Province, Northeast China. Forensic Sci Int: Genet 17:153–154. https://doi.org/10.1016/j.fsigen.2015.05.008
Guo F, Zhang L, Jiang X (2015) Population genetics of 17 Y-STR loci in Xibe ethnic minority from Liaoning Province, Northeast China. Forensic Sci Int: Genet 16:86–87. https://doi.org/10.1016/j.fsigen.2014.12.007
Kang LL, Liu K, Ma YM (2007) Y chromosome STR haplotypes of Tibetan Living Tibet Lassa. Forensic Sci Int 172(1):79–83. https://doi.org/10.1016/j.forsciint.2006.08.010
Zhu BF, Wu YM, Shen CM, Yang TH, Deng YJ, Xun X, Tian YF, Yan JC, Li T (2008) Genetic analysis of 17 Y-chromosomal STRs haplotypes of Chinese Tibetan ethnic group residing in Qinghai province of China. Forensic Sci Int 175(2):238–243. https://doi.org/10.1016/j.forsciint.2007.06.012
Shan WJ, Ablimit A, Zhou WJ, Zhang FC, Ma ZH, Zheng XF (2014) Genetic polymorphism of 17 Y chromosomal STRs in Kazakh and Uighur populations from Xinjiang, China. Int J Legal Med 128(5):743–744. https://doi.org/10.1007/s00414-013-0948-y
He J, Guo F (2013) Population genetics of 17 Y-STR loci in Chinese Manchu population from Liaoning Province, Northeast China. Forensic Sci Int: Genet 7(3):e84–e85. https://doi.org/10.1016/j.fsigen.2012.12.006
Wu FC, Ho CW, Pu CE, Hu KY, Willuweit S, Roewer L, Liu DH (2011) Y-chromosomal STRs haplotypes in the Taiwanese Paiwan population. Int J Legal Med 125(1):39–43. https://doi.org/10.1007/s00414-009-0416-x
Wen B, Li H, Lu D, Song X, Zhang F, He Y, Li F, Gao Y, Mao X, Zhang L, Qian J, Tan J, Jin J, Huang W, Deka R, Su B, Chakraborty R, Jin L (2004) Genetic evidence supports demic diffusion of Han culture. Nature 431(7006):302–305. https://doi.org/10.1038/nature02878
Acknowledgments
We are grateful to all sample donors. This study was supported by grants from the Health Technological Innovation Program of Jilin Province (No. 2016J025).
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Figure S1
MDS plot for all studied populations. All samples of Han population were indicated by red dot. (GIF 51 kb)
Figure S2
MDS plot for Han population only. Only the newly reported Jilin samples were indicated by red dot. (GIF 71 kb)
Table S1
Y-STR haplotypes of 1026 Changchun Han males included in this study (XLSX 102 kb)
Table S2
Statistics of haplotype diversity, haplotype match probability, and discrimination capacity parameters for all studied populations (XLSX 12 kb)
Table S3
Population differentiation between all studied populations (XLSX 19 kb)
Table S4
Population differentiation between Han populations (XLSX 12 kb)
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Chen, WQ., Feng, Z., Jin, W. et al. Genetic analysis of 17 Y-STR loci from 1026 individuals of Han populations in Jilin Province, Northeast China. Int J Legal Med 132, 1309–1311 (2018). https://doi.org/10.1007/s00414-018-1778-8
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DOI: https://doi.org/10.1007/s00414-018-1778-8