Abstract
Abiotic stresses such as salinity, drought, extreme temperature, radiation, nutrient deficiency, and heavy metals can significantly impact plant growth and yield, posing a challenge to agriculture. Various strategies have been employed to enhance plant resistance to these stresses through conventional and genetic manipulations. However, these often require extensive breeding programmes and substantial financial investments. In this regard, plant root exudates have emerged as a promising tool for improving plant performance under adverse conditions. They play a crucial role in the rhizosphere, influencing and guiding the microbial community to support plant growth. Published literature suggests that plants can adapt to abiotic stress by modifying the chemistry of their root exudates. These modified exudates can recruit beneficial microorganisms that aid in protecting plants from stress. Despite the progress made in identifying, characterizing, and detecting root exudates, there is still a substantial gap in our understanding of their significance and potential applications, particularly in addressing abiotic stress in plants. Therefore, the present review provides valuable insights into recent research progress in the field of root exudates. It covers fundamental information about root exudates, mechanisms of exudation, the impact of abiotic factors, and the interactions between roots and microbes in exudation. The review also explores how root exudates contribute to enhancing abiotic stress tolerance in plants, shedding light on the potential benefits of these compounds. The review also discusses existing challenges in the field of root exudates, particularly in their application for sustainable agriculture. It also addresses potential research gaps and outlines future directions for further investigation and practical application in agriculture for abiotic stress amelioration.
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Introduction
Abiotic stress is one of the prime environmental perturbations that negatively influence plant growth and development, with a significant impact on total crop production (Dwivedi and Sopory 2023; Khan et al. 2021). Generally, abiotic stresses are comprised of extreme temperature (both heat and cold), salinity, drought, flooding, nutrient deficiency, and heavy metals. These stresses have been reported to cause a significant decline (> 50%) in the total yield of almost all the major food crops grown throughout the world (Kashyap et al. 2017). It has been reported by several workers that the changing climatic conditions aggravate the negative effects of abiotic stresses on food crops with time by altering plant root growth, which further decreases crop growth by restricting the sufficient supply of nutrients and water in the phyllosphere (Fig. 1). Consequently, these will also create a major threat to the global food and nutritional security (Kosová 2018). Among various abiotic stresses, drought stress negatively impacts plant growth by altering stem elongation, leaf expansion, root proliferation, and foremost, a decline in water and nutrient uptake (Singh et al. 2023). In addition, a reduction in the leaf water potential and stomatal closing and opening, a delay in flowering, and a reduction in seed number and size are also observed in drought-stressful environments (Xu et al. 2019). Similarly, in the case of salt stress, prolonged harmful effects on glycophytes have been noticed (Zhao et al. 2021a, b; Negrão et al. 2017). The majority of the plants cannot tolerate salt concentrations beyond 200 mM (Khan et al. 2021), as it affects seed germination, seedling establishment, and vegetative growth. Further, published literature also mentioned the rise in osmotic pressure along with ion toxicity behind the oxidative damage of plants in saline environments (Subiramani et al. 2020; Kumari et al. 2019). Another category of abiotic stress, i.e., heat stress, is also reported to influence the physiology, biochemistry, and morphology of the plant and is ultimately responsible for stunted plant growth with a significant drop in plant biomass and productivity (Morcillo et al. 2021; Kashyap et al. 2018). Similarly, heavy metals have been reported to impose diverse types of direct and indirect influence on plant growth (Kushwaha and Kashyap 2021). More specifically, reduction in plant growth and commotion of various physiological and molecular phenomena, along with the culmination of chlorosis, senescence, and suppression of chlorophyll and photosynthesis activities, have been reported as a penalty of heavy metal stress (Bhat et al. 2020).
A growing body of research literature acknowledges the rhizosphere as a hub of microbes that supports large and metabolically active groups of microbes (Pandiyan et al. 2022; Solanki et al. 2012; Kushwaha et al. 2021). Khan et al. (2021) highlighted that the rhizosphere occupies millions of microbial cells; however, their composition is significantly influenced by plant growth stages and the type of genotype. The credit for the first observation concerning the abundant presence of microbes in the rhizosphere region rather than in distant soil goes to Hiltner (1904). Later on, various indications in connection with root exudation and microbial abundance in the rhizosphere were made by Knudson (1920) and Lyon and Wilson (1921). Recently published reports indicated that in the rhizosphere, plant roots release miscellaneous types of compounds (Table S1) that serve as food and chemical attractants for diverse types of soil microbes (Kumar et al. 2022; Feng et al. 2021; Pandey et al. 2017). It has been noticed that these root exudates have the ability to manipulate the physicochemical characteristics of the soil and thus play an important role in shaping the microbial community structure in the rhizosphere (Zhang et al. 2021; Bechtold et al. 2018). Consequently, root exudates are also reported to enhance plant growth in stressful environments by convalescing the nutrients and water uptake from the soil (Ma et al. 2022a; Ahkami et al. 2017). More specifically, they increase the level of phosphate and nitrate reductase activities under water-stress regimes while reducing the Na+ accumulation in saline environments (Wang et al. 2016). Besides abiotic stress amelioration, root exudates are also reported to contain soil-borne pathogens that impede plant defence systems (Zhou et al. 2023a, b; Chen et al. 2022; Baetz and Martinoia 2014). Briefly, in the current review, attempts have been made to recapitulate the recent progress attained in the field of root exudates. The article provides fundamental information on root exudates, mechanisms of exudations, effects of abiotic factors, and root-microbe interactions in exudation, along with the role of root exudates in convalescing abiotic stress tolerance capabilities in plants. Finally, existing challenges in the field of root exudates for their application in agriculture sustainability, along with future directions, are outlined.
Root Exudation Phenomenon
Root exudation is one of the prime events in the rhizo-deposition process and is a key source of soil organic carbon liberated by the roots of plants (Panchal et al. 2022). Chai and Schachtman (2022) mentioned that root exudation contributed up to 21% of the plant net photosynthates; however, their contribution greatly relies on the soil nutrient status, plant species, and plant growth stage (Hayat et al. 2017). Usually, root exudates represent both high molecular weight compounds (e.g., proteins and mucilage, etc.) and low molecular weight compounds, more assorted types than high molecular weight compounds (Table S1). It is important to mention here that the low-molecular-weight compounds contained a broad series of primary metabolites (e.g., amino acids, carboxylates, sugars, etc.) and secondary metabolites (e.g., coumarin, flavonoids, sorgoleone, etc.) (Gargallo-Garriga et al. 2018). Plant root exudates perform several functions, majorly linked with plant performance under stressed environments (Chai and Schachtman 2022). They also act as a vital carrier compound for the movement of material and the exchange of information between plant-soil interfaces. Moreover, they are also acknowledged as the prime players in the establishment and maintenance of the vitality and function of the rhizosphere micro-ecosystem by serving a vital part in the rhizosphere material exchange. Most importantly, they help improve the bioavailability of soil nutrients to plants (Zhao et al. 2021a, b). The carboxylates in root exudates are well recognized for their chelating properties, which help in the solubilization of phosphorus (P) for plant uptake. Besides altering soil nutrient status, root exudates also facilitate plant root-microbe interactions and help in crop growth augmentation under stressful conditions (Chai and Schachtman 2022; Solanki et al. 2022). For instance, DIMBOA (2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one), identified as one of the key maize root exudates, is reported to serve as a chemo-attractant for plant growth-promoting rhizobacterium (Pseudomonas putida KT2440) as well as offer allelopathic action against soil-borne pathogens and nearby plants (Neal et al. 2012). A list of different types of compounds released by plant roots as exudates is mentioned in Table S1.
The exudation of low molecular weight carboxylates having ≤ 3 carboxyl groups (e.g., malate, citrate, and oxalate, etc.) by roots is an important plant strategy to raise phosphorus (P) uptake by displacing insoluble P from P-containing organic and inorganic compounds in soil and making them accessible to roots (Fig. 2). The potential of carboxylates to release P is highly dependent on the concentration of carboxylates and the presence of carboxyl groups with tricarboxylates (citrate, aconitate, etc.). These have a high influence on P-desorbing, while monocarboxylates (acetate, pyruvate, etc.) have a low impact (Chai and Schachtman 2022). For instance, in the case of Arabidopsis thaliana and wheat, cultivars with higher citrate efflux have been found to be relatively more resistant to P-stress relative to genotypes displaying lower citrate efflux (Ryan et al. 2014). Further, it has been observed that the process of rhizosphere acidification operates synergistically with root exudates having carboxylates to convert the organic P (Po) and inorganic (Pi) pools. The root exudates with phenolics secrete P from the Pi pool by chelating with P-containing minerals (e.g., Fe/Al-hydroxides). In addition to this, acid phosphatases (APases) liberated P from P-containing organic matter at low pH (Chai and Schachtman 2022). However, the role of transporters responsible for phenolics and acid phosphatase efflux still needs to be investigated.
Recent research indicated that rhizosphere acidification and root exudation of carboxylates are the prime processes involved in the solubilization of P and are indirectly associated with the rise of P-solubilizing bacteria in the root zone (Chai and Schachtman 2022). Furthermore, it was also observed that phosphorus deficiency positively correlated with stimulation of the phenolic exudation in the rhizosphere (De Andrade et al. 2022). Hu et al. (2005) documented that the phenolic compounds (e.g., caffeic and protocatechuic acid) assist in P desorption by serving as a binder in the soils containing P minerals and thus help in releasing P for plant uptake, as depicted in Fig. 2. The exudation of phenolic compounds is also reported to suppress the abundance of the microbial communities dwelling in the rhizosphere and, in turn, struggle with plant roots for P nutrition. In addition to this, P deficiency is also demonstrated to persuade acid phosphatases (APases) to release into the rhizosphere (Nuruzzaman et al. 2006). At low pH, APases aid in the release of P from organic P pools (Hurley et al. 2010). APases hydrolyze the organic P content present in the rhizosphere, which later mobilises in the form of orthophosphate (after conversion from carboxylates) before its absorption by the plant roots (Fig. 2). However, the amount of APase secretion in the root zone depends on the plant species. Nuruzzaman et al. (2006) observed a higher level of secretion of APases in tomato and white lupin, while a low amount of APases was released in the case of rice, wheat, and maize.
Regulation and Factors Affecting Root Exudation
At the root-soil interface, it has been observed that the root is constantly exposed to diverse types of stress factors of both biotic and abiotic nature. In turn, roots respond to these stresses by secreting a series of diverse types of chemicals, generally mentioned in literature as root exudates. These chemicals protect roots against the adverse effects of these stresses and support favourable relationships. Mechanical impedance due to soil compaction and mild drought conditions has been reported to upset the root morphology and result in the stimulation of root exudates’ secretion (Gargallo-Garriga et al. 2018; Oliveros-Bastida et al. 2012). Under in-vitro conditions, alterations in the composition of root exudates of a particular plant species have been noticed in response to diverse types of growth media and support the notion regarding the role of released and available nutrition in the regulation of the root exudation process. Badri and Vivanco (2009) mentioned that nutrient deficiency is also positively linked with the augmentation of the exudation process of metabolites, specifically those that make the nutrients available to the roots. Besides this, temperature also serves as an important factor in the modulation of the root exudation process. This fact is well supported by the study of Bekkara et al. (1998) who reported slow exudation of tannins and phenolic compounds at low temperatures (4 °C) compared to the exudation at 30 °C in the case of Vicia faba hosts.
Elicitors are another class of molecules that are reported to encourage defence or stress-stimulated responses in plants (Meena et al. 2023; Kashyap et al. 2022). In nature, they are available in both chemical and physical forms. Badri and Vivanco (2009) mentioned that elicitors secrete phytochemicals at much higher concentrations than non-elicited plants to provide support for plant roots. Furthermore, a series of compounds normally not released in the root exudates of non-elicited plants have been observed in elicited plant root exudates. Interestingly, it has been reported that even a single elicitor has the potential to release distinct types of compounds in a single plant species (Kashyap et al. 2021). It has been noticed that the exogenous application of defence signalling molecules [e.g., salicylic acid (SA), methyl jasmonate (MeJA), nitric oxide (NO), etc.] resulted in the accumulation of a large number of secondary metabolites (Zhao et al. 2005; Kandoudi and Németh-Zámboriné, 2022). Similarly, mineral deficiency was also reported to induce the intrinsic synthesis of elicitor molecules involved in the plant signaling rejoinder. Schachtman and Shin (2007) mentioned that the deficiency of potassium leads to the induction of a JA-mediated defence mechanism. Generally, the signaling molecules are released in different parts of the plant, as SA and MeJA are noticed in the medium of cultured plant cells (Chen et al. 2001), while accumulation in the apoplastic space of plant roots has been reported in the case of nitric oxide (NO) (Stohr and Ullrich 2002). In one such study, Noritake et al. (1996) observed that NO triggers the accumulation of phytoalexin (e.g., rishitin) in the potato tuber tissues and the root exudates. Studies have also proved that the application of SA, MeJA, and NO resulted in the rise of root exudation of phytochemicals in Arabidopsis plant roots (Badri et al. 2008a). Various secondary metabolites released in response to SA, MeJA, and NO elicitors are shown in Table S2.
Mechanism of Root Secretion
Plant roots exude a series of compounds by following a passive process arbitrated by three distinct pathways (Badri and Vivanco 2009). These include diffusion, ion channels, and vesicle transport (Fig. 3). Sanders and Bethke (2000) described that during the diffusion process, small polar and uncharged molecules flow through permeable lipid membranes. However, Badri and Vivanco (2009) observed that the diffusion process is greatly influenced by membrane permeability and cytosolic pH. Besides this, other compounds such as amino acids, sugars, and carboxylate anions are also moved across the membranes with the support of proteins. The direction of the movement of the compounds is determined by their electrochemical gradient, which permits the compounds to pass from the cytoplasm of root cells (mM concentration) to the soil (µM concentration). In another similar study, Samuel et al. (1992) have shown that the high level of the cytosolic K+ diffusion potential and the proton extrusion with the help of ATPase enzymes determined the positively charged gradient, which in turn was responsible for the quick release of carboxylate anions. Besides this, membrane integrity is another factor that also influences the pace of organic acid release in the root zone. Likewise, the anion channels also play a key role in controlling the release of these compounds in the root region (Badri and Vivanco 2009). Lee et al. (2007) mentioned that the secretion of sugars, amino acids, and metals from root cells is greatly determined by a specific type of transporter. It is important to mention here that plants have a unique system of metal homeostasis that helps in avoiding the excessive accretion of free metal ions (e.g., Mn, Zn, Fe, and Cu, etc.). The metal homeostasis mechanism involves the coordination of metal ion transporters, which predominantly engage in the uptake, translocation, and compartmentalization of metal ions. For instance, in the case of graminaceous species, it has been observed that mugineic acid (MA) secreted from the roots into the rhizosphere serves as a metal-binding ligand and results in the formation of Fe (III)-MA ligand to lessen the Fe toxicity. Later, in the case of the maize host, Badri and Vivanco (2009) identified that the Fe (III)-MA ligand penetrates the root cells controlled by a specific yellow stripe-like (YSL) transporter.
Generally, the excretion of high-molecular-weight compounds from roots depends on the workings of the vesicular transport system (Battey and Blackbourn 1993). Although the mechanism of the vesicle-mediated movement of proteins is well explained by Field et al. (2006), the transport mechanism of phytochemicals is still not completely deciphered (Grotewold 2004). However, the available information provides clear indications regarding the involvement of vesicle-mediated transport of phytochemicals in the leaf cells (Gani et al. 2021), but except for the golgi-mediated transport of mucilage polysaccharides across the root cap, the information about the mechanisms of phytochemical secretion from root cells is still under discovery (Driouich et al. 2021; Neumann and Romheld 2007).
ABC Transporters in Root Exudation Processes
Earlier published reports indicated that the ATP-binding cassette (ABC) transporters and multidrug and toxic compound extrusion (MATE) transporters are the major players responsible for the movement of defence-related phytochemicals inside the plant cells (Gani et al. 2021; Panthri and Gupta 2022). Generally, ABC transporters obtain energy for primary transport via ATP hydrolysis, while MATE transporters operate by H+ gradient-dependent secondary transport. Interestingly, both of these groups play noteworthy roles in the trafficking of flavonoids to the vacuole (Yazaki 2005). It is important to mention that the ABC transporters are one of the largest protein families and are reported in all the phyla. Sanchez-Fernandez et al. (2001) classified plant ABC proteins into 13 subfamilies based on four different parameters. These include protein size (half or full), idiotypic trans-membrane/linker domains (presence or absence), orientation (backward or forward), and sequence similarity. Based on characteristic attributes of the molecules associated with ABC transporters in plants, they are categorised into three major subfamilies and reported in the literature as pleiotropic drug resistance protein (PDR), multidrug resistance P-glycoproteins (PGP), and multidrug resistance-related protein (MRP). Later, it was reported that these ABC transporters play an essential role in various cellular processes (Stein et al. 2006). Most remarkably, they served as the determinant for seepage of potentially toxic compounds, lipid translocation, heavy metal tolerance, disease resistance, nutrient transport, salt stress, etc. (Banasiak and Jasiński 2022).
Generally, the secretion of plant secondary metabolites from plant roots is driven by ATP-dependent processes, which further indicates the involvement of ABC transporters in these processes. It has been noticed by Loyola-Vargas et al. (2007) that root secretion profiles of Arabidopsis differ significantly in composition under the influence of different types of inhibitors (e.g., potassium cyanide, nifedipine, sodium orthovanadate, verapamil, quinidine, glibenclamide, etc.), which hint towards the involvement of active transporting systems (i.e., ABC transporters and P-type ATPases) in the root secretion process because all the inhibitors used in the study possess ATP pool-deteriorating characteristics in the cell. Moreover, another independent study by Sugiyama et al. (2007) also revealed that the secretion of genistein (a signal flavonoid released by soybean roots during rhizobium symbiosis) is regulated by an ABC transporter in an ATP-dependent manner. Further, they also observed the non-repression of genistein secretion in soybean root by other inhibitors, specifically those responsible for the inhibition of plasma membrane ionophores (e.g., nigericin, valinomycin, and gramicidin). Besides this, Badri et al. (2008b) also documented the function of ABC transporters in the root secretion process by performing ABC transporter protein knock-out experiments in Arabidopsis root cells. They also noticed three different compounds transported by different ABC transporters, and among them, one such compound was documented as 3-hydroxy-4(Z), 6(Z), 8(Z), 10(Z)-tetraenoic acid, which further strengthened the notion that a single ABC transporter can shift structurally distinct compounds or that one compound can be transferred by distinct types of ABC transporters. However, we felt that further research and investigation to discover other transporting systems (e.g., MATE) and different substrates that trigger root secretion of phytochemicals are obligatory to harness the agricultural and biotechnological advantages of root exudation.
Release and Movement of Primary Metabolites at Root Tip Interface
Root exudation includes all the processes that deal with the transportation of carbon to roots and their exudation into the soil (Yin et al. 2023). The carbon produced at the photosynthesis site in plants is transported through the phloem with the assistance of the pressure-driven mechanism of phloem flow, as explained by Münch (1930). In this mechanism, the difference in turgor pressure between sink and source organs plays an essential role in the transportation of metabolites and is generally regulated by the generation of concentration gradients and source-sink relationships. De Schepper et al. (2013) mentioned that during the unloading of metabolites, low-molecular-weight proteins and solutes transferred to the phloem-pole pericycle with the help of funnel plasmodesmata, as depicted in Fig. 4. During this process, proteins travel in discrete pulses, usually called ‘batch unloading’, and their movement is limited to the phloem-pole pericycle only. However, the unloading of low-molecular-weight organic solutes takes place without restrictions, travels out of the phloem-pole pericycle, and is quite helpful for root exudation at the root tip region. Later, this mechanism was well supported by the work of Ross-Elliott et al. (2017), who also described root exudation at the root tip region as the prime course for the movement of all types of solutes during unloading and their further diffusion towards the surrounding cells because of the high degree of plasmodesmata networks in the region (Fig. 4).
Principally, the symplastic pathway is one of the key channels that help in the free movement of the metabolites within the plant system. Generally, metabolites enter the soil environment with the help of the exudate plasma membrane. Yang and Hinner (2015) mentioned that the plasma membrane helps in the easy movement of small molecules (e.g., urea, glycerol, etc.) and the exchange of gas. Contrarily, they also pointed out that the penetration and transfer of ions and large-size uncharged polar molecules such as glucose do not operate through the plasma membrane, and in such cases, specific trans-membrane proteins in the plasma membrane help in their smooth movement inside the plant system. Later, Sasse et al. (2018) also observed that small pores through the lipid bilayer provide passage for large-polar or charged molecules to move across the membrane without making any physical contact with the hydrophobic fatty acid chains present on the membrane phospholipids. Further, it has been observed that the fine-tuning of the exudation flux of sugars, amino acids, and organic acids largely depends on the positive and negative regulation of the genes along with the post-translational level alteration of the specific efflux carriers and channels (Mishra et al. 2022; Badri et al. 2008a). At present, the major types of well-characterized efflux transporters of primary metabolites include amino acid transporters [UMAMIT transporters, CAT transporters, GDU transporters, sugar transporters (majorly related to the SWEET transporter family)] and organic acid transporters [ALMT/malate and MATE/citrate transporters] (Manck-Götzenberger and Requena 2016; Dinkeloo et al. 2018; Mora-Macías et al. 2017). More interestingly, the majority of these transporters are not directly coupled to ATP hydrolysis or ATP-dependent H+ pumps and H+ antiports. However, Badri et al. (2009) documented that ATP-dependent ABC transporters for secondary compounds and MATE/citrate transporters have H+-coupled antiport activity (Meyer et al. 2010), which is different from other efflux transporters and usually not coupled to ATP hydrolysis or to H+ co-transport. The most common characteristic of the majority of the primary metabolite transporters is their role as substrate-specific facilitators, which helps in rapid primary metabolite diffusion across the plasma membrane via trans-membrane carriers along the concentration gradient (Sasse et al. 2018). In this regard, Mora-Macias et al. (2017) have proved that P-deficiency or Al3+ toxicity stimulates malate exudation by up-regulating ALMT in the roots. It has been observed that after the release of metabolites from the phloem cell plasma membrane, the apoplastic transport pathway activates to release the metabolites into the soil as exudates. This observation is well supported by the study made by Vidal et al. (2018), who demonstrated that in immature wheat roots, the flow of carbon-containing compounds from the stele to the cortex follows an apoplastic pathway and is later utilised by microbial communities dwelling in the soil environment. Further, it has been noticed that the diffusion process in the apoplast is the prime process helping in the regulation of flux from plant roots to the soil environment without getting any intrusions of plasma membranes at the root tip. Somssich et al. (2016) mentioned that the endodermis cells provide a barricade against apoplastic diffusion, which is also quite evident from the fact that the meristem lacks a Casparian strip, which usually serves as an apoplastic barrier, as depicted in Fig. 4. Overall, we felt that the knowledge of the contributing efflux carriers for the synthesis of primary metabolites of the exudates at the soil-root interaction zone can help in generating new information about the root exudation regulation mechanisms.
Role of Root-Rhizosphere Interactions in Root Exudates Exudation
The root tip is the prime place, where the majority of the root exudation activities take place (Sasse et al. 2018). It is important to mention here that the root tip is the foremost important plant part that is in direct contact with the new soil environment, which in turn plays an important function in regulating root responses under the pursuance of environment-driven stimuli (Tedone et al. 2020). The root-soil interaction zone in the rhizosphere is the prime place for plant-microbial interactions, where both advantageous and detrimental microorganisms reside together. Pérez-de-Luque et al. (2017) mentioned that the microbial community structure in the rhizosphere is highly dynamic and influenced by myriads of factors, including soil nutrition, plant root architecture, type of plant species, environment, etc. Furthermore, it has been noticed that the rhizobacteria interactions in the root zone (i.e., rhizosphere) introduce various types of intuitive impact on soil health by affecting the soil nutrient status, heavy metal remediation processes, soil immune responses, and root growth (Badri et al. 2009). Later, it was reported that the interactions between roots, rhizosphere, and microbial communities modulate plant root growth and their proliferation, which ultimately play a significant role in regulating the exchange of resources between the soil environment and the upper section of plants, i.e., the shoot (Zhang et al. 2017). The root-root-rhizobacterial interactions also promote root exudation, which promotes the attraction of microorganisms towards the root vicinity (Semchenko et al. 2014). Besides, root exudates also mediate plant–microbe interactions by acting as food and chemo-attractants for different types of microbial associations, which further assists in root colonisation and plant growth promotion. In another independent study, Neal and co-workers (2012) noticed that the presence of Pseudomonas putida in the maize rhizosphere helps in benzoxazinone removal from the rhizosphere. Later, Dilnashin et al. (2020) documented that microbial soil diversity is greatly influenced by root exudate composition. The list of selected bacteria with a role in root exudation enrichment and amelioration of abiotic stress in cereals has been presented in Table 1.
A series of published reports indicated that organic acids are one of the prime components of root exudates (Xiong et al. 2023; Fujii et al. 2021; Sharma et al. 2020). Organic acid not only serves as a source of energy for regulating microbial metabolism at the cellular level but also serves as one of the intermediary compounds for regulating the bio-geochemical processes of various cycles operating in the rhizosphere. It has been noticed that low-molecular-weight carbon (C) compounds of root exudates play an important role as a precursor in the biosynthesis of rhizobacterial phytohormones. For instance, tryptophan (Trp) is one such compound that is mostly present in the root tip region and helps in IAA production as a precursor (Li et al. 2018a, b). Similarly, the oozing of aminocyclopropane-1-carboxylic acid (ACC) from plant roots has been mentioned in earlier published reports (Xiang et al. 2019). It is important to mention that this compound serves as a precursor for ethylene synthesis and is utilised as a carbon and nitrogen source by the bacteria dwelling in the rhizosphere. Besides the aforementioned compounds, the oozing of flavonoids from the roots of leguminous crops and their significance in inducing transcription of Rhizobia Nod factors (NFs) have been established (Sugiyama 2019). Functionally, NFs are required by the roots of leguminous crops for the formation of root hairs and nodule initiation. It is evident from various reports that root-rhizosphere and rhizobacterial interactions operate in a mutual communication mode between plants, soil, and microorganisms (Wang et al. 2020). Further, it has been noticed that all the aforementioned interactions and communications are of a convoluted type and play an indispensable role in determining the positive outcome in terms of soil health and plant fitness. Rahimi et al. (2020) explained that the root-rhizosphere and rhizobacterial interactions are highly advantageous to plants in terms of nutrient acquisition from soil and significant enhancements in plant growth, grain quality, and total yield. In addition to this, plant rhizobacteria help to improve nutrient cycling in the rhizosphere and tolerance capabilities in plants to resist unfavourable environmental conditions.
The term ‘DefenseBiome’ is widely used in published literature in connection to the plant-associated microbes that boost plant growth in stressed environments. Further, Liu et al. (2020) also pointed out that the scope of ‘DefenseBiome’ is much wider and currently widely used in the context of the development and design of functionally consistent and reliable synthetic microbial communities for better and more recuperative plant health. In recent times, DefenseBiome’s concept has been explored to gain deep insight into plant-microbial interactions as well as to invent new and novel plant probiotics (Liu et al. 2020). This concept got strength from the findings of Sharp et al. (2011), where they noticed lesser emanation of ethylene from mature foliage of Cytisus × praecox under drought stress post-inoculation of Variovorax paradoxus 5C-2. In addition, they also observed a significant reduction in the abscission of mature leaves. Similar, favourable effects of the rhizobacterium inoculation have been reported in Fargesia murielae against wounding or injury due to divisional propagation, as well as in the case of Aquilegia × hybrids under drought stress regimes. The affectivity of microbial partners in plant–microbe interactions and their usefulness in supporting plant growth under abiotic stress conditions depend upon several biochemical and physiological features of the interacting microbes, and a major among them is their survivability. Pathania et al. (2020) mentioned that Bacillus MT7 associated with the maize rhizosphere offered better survivability as well as supporting tomato growth under a series of abiotic stressors such as drought, temperature, heavy metals, salt, etc. Further, they also noticed that the colonisation ability of Bacillus MT7 was also greatly influenced by their potential to act in response to tomato root exudates and biofilm formation for better establishment and proliferation in the tomato rhizosphere, along with the expression of diverse types of plant growth-promoting attributes (Zhou et al. 2016). More specifically, under alkaline conditions, Paenibacillus polymyxa BFKC01 facilitates Fe mobilisation by stimulating the production of phenolic compounds in the root exudates of Arabidopsis. It also produces auxin, which improves the plant root system and consequently enhances Fe acquisition from the soil. Consequently, it leads to higher endogenous Fe content in plants and enhanced photosynthesis in an alkaline environment (Zhou et al. 2016). Later, Woo et al. (2020) recorded that Bacillus subtilis strain GOT9 stimulates the expression of drought and salt-inducible genes and, as a result, plays a significant role in promoting lateral root growth and development under drought and salt stress in the case of Arabidopsis and Brassica hosts (Woo et al. 2020). Similarly, the transcriptome analysis of Photorhabdus luminescens performed by Regaiolo et al. (2020) also indicated the role of a series of genes involved in chitin degradation, biofilm regulation, the formation of flagella, and the type VI secretion system while interacting with the root exudates. They also noticed that P. luminescens suppressed the growth of plant pathogens and exhibited specific interactions with plant roots (Regaiolo et al. 2020).
Several research documents indicated that the root exudate composition is greatly influenced by the genotypic characteristics of the plant species and stress type (Vives-Peris et al. 2020; Ulrich et al. 2022). Further, it has been noticed that root exudate composition also plays a significant role in regulating the growth of rhizobacteria in the rhizosphere (Feng et al. 2021; Sun et al. 2021a, b). For instance, the study of Vives-Peris et al. (2018a, b) highlighted that the root exudates from tolerant genotypes for salt and drought stresses stimulate the growth and colonization of Pseudomonas putida KT2440 and Novosphingobium sp. HR1a in comparison to the exudates released by salt-sensitive genotypes. The two bacteria isolated from tolerant genotypes for two stresses also displayed a high level of β-galactosidase activity, proline, and salicylates in root exudates of tolerant genotypes, further strengthening the potential of these rhizobacteria to serve as bio-indicators of stress in the citrus host. Moreira et al. (2020) observed that the application of P. reactans EDP28, Pantoea alli ZS 3–6, and Rhizoglomus irregulare resulted in a remarkable increase in biomass and nutrient status of maize. It is ascribed to the significant rise of K+ and Na+ reduction in corn plants under saline stress, revealing the salinity-abating attributes of these tested microbes (Moreira et al. 2020). Besides, the plant–microbe interactions help the plant develop a rhizosheath on the root tip, which helps the plant protect against abiotic stresses that include soil acidity, nutrient deficiency (N and P), and water stress. However, the rhizosheath characteristics are greatly influenced by the physiology and morphology of plant root systems. Besides this, the interactions between plants and microbes on the root surface also play a significant role in regulating rhizosheath characteristics. The role of exopolysaccharide-producing bacteria (EPB) in modulating root exudates and soil aggregation has also been highlighted by Ilyas et al. (2020). Further, they also mentioned that, as a consequence of this, the size of the rhizosheath increased, which further enhanced the minerals and water absorption abilities of the plants. Based on the aforementioned information, it can be concluded that any strategy based upon the association of gene networks regulating rhizosheath formation and root traits can become a prospective strategy for managing the adverse effects of climate change on agriculture.
Previous published literature indicated that under abiotic stress, plants make alterations in their root architecture by exudating specific metabolites through roots in the rhizosphere, which helps them combat the stress situation (Salem et al. 2022; Koprivova and Kopriva 2022). In one such situation of phosphorus deficiency, the tall fescue in the presence of Neotyphodium spp. as an endophyte has been found to abet the P-deficiency stress by making changes in the chemical composition and root architecture of the rhizosphere by exuding out phenolic-like compounds (Verma et al. 2021). Furthermore, the phenolic-like compounds were found to have Al-chelating activity, which promoted aluminium sequestration on the root surface (Malinowski and Belesky 2000). In the case of Capsicum annuum, inoculation with the mycorrhizal fungus (Glomus mosseae) increased the root area by approximately 50%, induced high numbers of lateral roots, and promoted plant growth. Further, mycorrhization helped the plants cope with glyphosate-induced abiotic stress by plummeting mitotic activity and mitotic index as well as cell length in root apices (Ronco et al. 2008).
A large array of mechanisms operating during plant–microbe interactions and offering tolerance against abiotic stresses has a significant contribution to the promotion of sustainable agriculture. In one such transcriptomic study involving Trichoderma parareesei and tomato root exudates, the results of the spatio-temporal analysis revealed the priming effect of T. parareesei strain T6 in stimulating tomato defence responses towards biotic and abiotic stresses by upregulating the expression levels of lipoxygenase 1 (LOX1), ethylene-insensitive protein 2 (EIN2), SOS1 and the salicylic acid-related PR-1 gene (Rubio et al. 2014). Further, they also noticed that the interaction was beneficial for both of the partners. In another independent study by Mendoza-Mendoza et al. (2018), it has also been observed that the presence of sucrose in root exudates plays a vital function in Trichoderma-root interactions. They also noticed that the production of sucrose in root exudates as a consequence of Trichoderma-root interactions stimulates induced systemic tolerance (IST) against diseases and insect pest infestation by triggering oxidative bursts, enhancing salicylic acid synthesis by plants, and rapid secretion of elicitor-like proteins by Trichoderma spp. A similar type of mutualistic endosymbiosis has also been noticed in the case of Piriformospora indica, which offers protection against drought in Andrographis paniculata under laboratory conditions and has also been found to support higher production of diterpenoid lactones (e.g., andrographolide) with strong pharmacological actions (Nair and Manjula 2020). Under drought stress conditions, the plants colonized with P. indica have been recorded to support plant growth as well as release high levels of antioxidant enzymes, high proline content, and low malondialdehyde accumulation in contrast to control plants.
Role of Root Exudates in Recruiting Beneficial Microorganisms
Recent studies emphasise the role of root exudates in plant–microbe interactions, specifically as a “cry for help” to recruit beneficial microorganisms and enhance plant defence under stress conditions (Rolfe et al. 2019; Yuan et al. 2023; Upadhyay et al. 2022; Liu et al. 2021; Bakker et al. 2018). It has been observed that the secretion of vanillic acid, fumaric acid, and p-coumaric acid from barley roots plays a role in persuading the colonization of Pseudomonas fluorescens CHA0 strains. This colonization triggers the rapid synthesis of 2,4-diacetylphloroglucinol (DAPG), contributing to the protection of barley from the invasion of Pythium ultimum (Jousset et al. 2011). Similarly, malic acid secretion by Arabidopsis thaliana roots is noted for recruiting Bacillus subtilis FB17, which aids in protecting Arabidopsis from Pseudomonas syringae infection (Yuan et al. 2018). Scopoletin is another metabolite that is identified as a crucial root exudate in the interaction between Arabidopsis thaliana and P. simiae WCS417. This compound helps to protect the host from fungal infections caused by Verticillium dahliae and Fusarium oxysporum (Stringlis et al. 2018a, b). Root secretion of benzonitrile, benzothiazole, dimethyl trisulfide, formic acid, and terpene-like compounds from tomato plants is observed under Fusarium oxysporum stress. These secreted compounds augment the root interactions with Bacillus sp., contributing to better resilience and defense mechanisms in tomato plants (Gulati et al. 2020). More recently, it has been documented that the roots of Panax notoginseng secrete cinnamic acid, 2-dodecenoic acid, and 12-oxo-phytodienoic acid metabolites under autotoxic ginsenoside stress. These secreted metabolites promote chemotaxis and colonisation of Burkholderia sp. B36 in the rhizosphere, ultimately enhancing the survival rate of P. notoginseng (Deng et al. 2023). These aforementioned points highlight the sophisticated and dynamic interplay between plants and their associated microbes, showcasing how root exudates play a crucial role in recruiting beneficial microorganisms and bolstering plant defence mechanisms.
Root Exudates in Mitigating Abiotic Stresses in Crop Plants
The role of root exudates in combating different abiotic stresses has been studied in several host plants (Table 2). Sun et al. (2021a, b) documented that the composition and quantity of root exudates were not only determined by the plant species and genotype but also relied on external environmental parameters. The impact of different abiotic stresses on root exudate production and their function in enhancing plant performance under different stress conditions is described in the following sections.
Drought
Drought is observed as one of the major abiotic factors that negatively limit plant growth and crop yield (Singh et al. 2023). It is estimated that agricultural land under extreme drought could rise to 30% by the end of the century under the current situation of climate change (Burke et al. 2006). Recently published reports indicated that drought stress greatly influences the root exudates and root-associated microbiomes in nature (Chen et al. 2022; Ulrich et al. 2022). Several published studies have revealed that the number of constituents (e.g., soluble sugars, amino acids, and organic acids) in root exudate composition increased with the rise in drought stress gradients (Ulrich et al. 2019; Song et al. 2012). Analogous reports of a significant rise in the composition of organic acids in maize root exudates (e.g., succinic acid, acetic acid, citric acid, lactic acid, and maleic acid, etc.) under drought stress have also been observed (Chen et al. 2022; Hummel 2010). Besides this, roots extract K+ from the soil, and it is exchanged with organic acids produced as root exudates. Several pieces of published literature have highlighted that potassium (K+) and organic acid in plant root exudates play a prime role in regulating osmotic stress under drought conditions (Chai and Schachtman 2022; Carvalhais et al. 2011). In the case of Quercus ilex, Gargallo-Garriga et al. (2018) have reported that a rise in the level of drought severity influenced 71% of the total metabolites in root exudates, where the majority of the secondary metabolites belonged to the alkaloids and terpenoids categories. It is interesting to reveal that the composition of the root exudates reallocates towards primary metabolites (~ 81% of the total metabolites) during the drought recovery stage. However, in severe drought situations, the alterations in the composition of root exudates become irretrievable, resulting in plant death. Increased terpenoid production has also been recorded in Camellia sinensis after exposing plants to diverse types of abiotic stress situations, including drought (Han-Chen et al. 2020). Rhizosheath, which serves as a vital aspect of plant endurance under drought stress, helps in providing strength to plants under drought conditions and is the consequence of the mucilage exudation by the plant roots. Mucilage helps in assembling the soil particles as well as in soil aggregate stabilisation around the roots (Ndour et al. 2020). Besides this, exopolysaccharides produced by several rhizobacteria also have a role in strengthening rhizosheath formation (Kaci et al. 2005). Canarini et al. (2016) demonstrated that sunflower plants under drought produce large amounts of exudates, but their composition remained unchanged. Contrarily, in the case of soybean plants, the profile of root exudates altered under drought stress, but the exudation rate remained unchanged. The drought stress was also reported to enhance root exudation of phytohormones (e.g., ABA) and organic osmolytes (e.g., proline, betaine, trehalose, and pinitol) (Gargallo-Garriga et al. 2018; Calvo et al. 2017). It is worth mentioning here that ABA regulates plant responses under both drought and saline stresses (Sah et al. 2016). Published reports also indicated that the exogenous treatment of ABA in the root tissues of rice and barley alters the expression of water channel aquaporins and root hydraulic conductivity (Ding et al. 2016; Sharipova et al. 2016). Besides this, plants minimise water loss under drought stress and sustain cell turgor through the intracellular accumulation of osmolytes (Sharma et al. 2019a, b). Various workers documented that the exudation of osmolytes and ABA in root exudates is due to their leakage from roots under drought conditions (Shi et al. 2015; Serraj et al. 2002). In the case of maize root exudates, an increase in the content of organic acids (succinic acid, malic acid, lactic acid, acetic acid, maleic acid, and citric acid) under drought has been observed by Song et al. (2012). Later on, Chen et al. (2022) also noticed a similar role of organic acids in providing osmotic stress tolerance in plants under drought. Besides these, a significant rise (~ 71%) in the release of organic carbon in the rhizosphere with major components of organic acids (succinic, fumaric, and malic acids) has also been documented by Vives-Peris et al. (2020). Similarly, in the case of Agropyron cristatum, it has been noticed that drought promotes a 71% higher release of organic carbon in the rhizosphere with higher quantities of succinic, fumaric, and malic acids. Besides the aforementioned reports, drought stress is also reported to enhance organic acid production by the corn roots, with malic acid as the major contributor, and to contribute to phosphate solubilization and plant growth promotion under drought stress conditions (Henry et al. 2007).
Salinity
Salinity stress is one of the prime concerns for sustainable crop production and is predicted to influence 20 and 30% of the total global arable and irrigated agricultural land, respectively (Bhardwaj et al. 2022; Kashyap et al. 2020). By 2050, it has been forecast that more than 50 percent of the global fertile agricultural soil will be salinized (Sharma et al. 2019a, b). Salinity negatively affects various aspects of plant development, including seed germination, plant growth, development, and yield (Zhang et al. 2020). Saline stress hampers the plant’s ability to carry out photosynthesis effectively. This is due to a decrease in the content of chlorophyll and carotenoids, distortion of the chloroplast ultrastructure, and damage to the photosystem II (PSII) system. These factors collectively reduce the plant’s capacity for photosynthesis (Pan et al. 2021). It has been observed that salinity also affects transpiration and gaseous exchange in plants, which reduces stomatal conductance, which is the ability of stomata to open and close, leading to decreased gas exchange between the plant and the atmosphere. Besides this, the presence of high salt levels in the soil lowers soil water potential and leaf water potential. This disrupts plant water relations and reduces the turgor pressure within plant cells, ultimately causing osmotic stress (Navada et al., 2020). Salinity also increases the production of reactive oxygen species (ROS) within plant cells, which can cause oxidative stress and lead to various forms of damage within plant cells, including lipid peroxidation, membrane deterioration, and damage to DNA and proteins (El Ghazali 2020). Generally, it has been found that saline soils represent a blend of salts of sodium, chloride, sulphate, magnesium, and calcium ions, with sodium chloride being the major player. Salinity causes the accumulation of ions such as sodium (Na+) and chloride (Cl) in plant tissues. This disrupts the uptake of essential ions such as potassium (K+) and calcium (Ca2+), leading to an ionic imbalance within the plant. This imbalance can have detrimental effects on various physiological processes (Isayenkov and Maathuis 2019). Yildirim et al. (2006) mentioned that salinity stress created a metabolic disorder in plants by enhancing Na+ uptake and lessening K+ and Ca2+ uptake. Besides this, the influence of salinity stress on the root exudation pattern of the plants is also observed (Vives-Peris et al. 2020). For instance, the level of flavonoids in root exudates of Phaseolus vulgaris and Glycine max increased when grown under saline stress (Dardanelli et al. 2010, 2012). Similarly, citrus root exudates exhibit enhanced levels of salicylates and proline in a saline environment (Vives-Peris et al. 2017). Singh and Jha (2016) also mentioned that the plants accumulate a series of osmolytes, for instance, proline, antioxidative enzymes, glycine betaine, and total soluble sugars, for acclimatisation under saline stress. Xie et al. (2020) conducted a study to investigate the impact of saline stress, specifically caused by sodium chloride (NaCl), on the amino acids present in the root exudates of Phragmites australis. The results of their research revealed that the plant may undergo biochemical changes in its root exudates, possibly altering the composition and secretion of total amino acids, as part of its strategy to cope with saline environments. Kumawat et al. (2022) reinforce the importance of organic molecules found in the root exudates of P. australis (e.g., trehalose, proline, acetylated glutamine dipeptides, carboxylamines, etc.) in helping halophilic microorganisms cope with salt stress. Under salt stress conditions, root exudates promote Ensifer meliloti CL 09 growth, which suggests that root exudates might be one of the factors for rhizosphere and endosphere microbial selection under salt stress (Moghaddam et al. 2016). In another study conducted by Lombardi et al. (2018), it was noticed that the root exudates released by plants under salt stress stimulate the growth of the beneficial rhizosphere fungus Trichoderma harzianum and act as chemoattractants.
Temperature
Temperature is another major factor that negatively influences plant growth and development (Walne and Reddy 2022; Hatfield and Prueger 2015). Several reports indicated that a rise of 3–4 °C in temperature could lead to a significant reduction of crop yield in the range of 15–35% in Asia, Africa, and the Middle East countries (Kashyap et al. 2018; Ortiz et al. 2008). A series of recent publications indicated that temperature influences both the quality and quantity of root exudates by regulating respiration, translocation, and photosynthesis in plants (Leuschner et al. 2022; Vives-Peris et al. 2020). Vives-Peris et al. (2018a) documented that the root exudates derived from heat-stressed plants grown at 30 °C do not reflect any major changes in the growth pattern of P. putida KT2440 and Novosphingobium sp. HR1a. However, root exudates derived from the plants grown at 40 °C showed a stimulatory impact on the growth of both of these bacteria. It was more pronounced in root exudates from the heat-responsive Macrophylla (Vives-Peris et al. 2018a). Besides this, the thermo-tolerant bacterium (P. putida AKMP7) also showed an array of plant growth-promoting attributes (e.g., production of HCN, ammonia, siderophore, phytohormones, P-solubilization, etc.) in addition to their contribution in conferring tolerance against heat stress in plants (Ali et al. 2011). It is worth mentioning that the plant growth-promoting bacteria release diverse types of growth regulators at the plant root interface that in turn help in stimulating root development and better absorption of nutrients and water from the soil surface (Zimmer et al. 1995). Recently, Tiziani et al. (2022) noticed that the composition of maize root exudates endures diverse types of modulation under heat stress in the root zone but is not significantly influenced by maize root types. Additionally, they also noticed that heat stress-specific exudates also alter the relative abundance of specific bacterial taxa, including beneficial microorganisms, in the host plants.
Nutrient Deficiecy
Plant developmental processes are greatly influenced by the availability of essential mineral nutrients in the soil. Plants primarily acquire mineral nutrients through the rhizosphere, which is the soil zone influenced by plant roots. In this region, microorganisms interact with plant products released in root exudates (Pantigoso et al. 2023). Mei et al. (2012) provided conclusive evidence regarding the role of root exudates in the symbiotic system of legumes, which usually requires a huge pool of P. They reported that the citrate secreted by the root chelates has the potential to enhance the N fixation capacity of root nodules in P-deprived soil (Mei et al. 2012). Similarly, Xia et al. (2016) noticed that the exudates of Michelia macclurei promote root growth and the establishment of Cunninghamia lanceolata by manipulating rhizosphere microflora for P acquisition. Other secondary metabolites, such as flavonoids and strigolactones, are also reported to facilitate plant association with mycorrhizal fungi under phosphorus-deficient conditions (Chai and Schachtman 2022). It has been noticed that in a nitrogen-deprived environment, root exudates assist plants in building strong associations with nitrogen-fixing microbes to check soil nitrogen losses through inhibition of the bacteria engaged in nitrification and denitrification processes. A series of research publications also indicated the potential of organic acid and acid phosphatase secretions from faba bean and chickpea roots in enhancing the absorption of P in maize plants (Zhang et al. 2020; Li et al. 2004). Similarly, Flavin’s exudation from Beta vulgaris also helps to improve Fe accessibility (Sisó-Terraza et al. 2016).
Organic acids (OAs) in root exudates are another vital player that contributes to protecting against plant stress because they can modify pH, behave as metal chelators, and also offer nutrients in the form of carbon sources for microbes (Panchal et al. 2021). Yang et al. (2019) observed that the high-potassium tobacco cultivar ND202 exuded a significantly higher level of available K content and organic acids in root exudates in comparison to common varieties, viz., K326 and NC89. Further, they also noticed that the ND202 cultivar maintained normal root vigour even under K-deficiency stress due to a rise in the production of stress-resistant related enzymes (e.g., POD, SOD, and CAT). The study by Gomez-Zepeda and colleagues (2021) also reported alternations in exudation rate and localization patterns of organic acids under P stress in the roots of Arabidopsis plants by using very advanced techniques able to detect organic acids at sub-micromolar levels. The authors felt that these findings are relevant for developing plant genotypes with augmented Pi-uptake. Besides the aforementioned reports, Javed et al. (2020) also documented the function of silicon (Si) application in abating the negative impact of cadmium (Cd) injury in Ajwain (Trachyspermum ammi). They also noticed that Si treatment also helps in enhancing the plant biomass, growth, antioxidant enzymes, photosynthetic apparatus, and mineral uptake in T. ammi. Besides this, a reduction in the exudation of organic acids and oxidative stress indicators in the stem and roots of T. ammi plants has also been noticed. In summary, the aforementioned reports established the significance of root exudates in stress amelioration and further highlighted the need for a better understanding of the pathways and mechanisms involved in the root exudate secretion that help in ameliorating plant stresses. Besides this, the merits of primary and secondary metabolites released from root exudates also need to be established as one of the prospective options for crop improvement as well as for abiotic stress amelioration for agricultural sustainability under climate change.
Light
Light has an important role in the root exudation process (Guo et al. 2023; Martin et al. 2018). Vives-Peris et al. (2020) mentioned that during the photosynthesis process, a part of the carbon-dioxide fixed is exuded out as root exudates because the process of photosynthesis is greatly influenced by light wavelength and photoperiod. For instance, Crocus sativus exposed to a longer photoperiod (14 h) released a higher amount of organic acids (e.g., benzoic and 4-hydroxybenzoic acids) in comparison to plants exposed to a short photoperiod of 10 h (Pramanik et al. 2000). Whereas, the circadian rhythm does not influence the root exudation process (Badri et al. 2010). He et al. (2016) noticed that the enhanced UV-B exposure led to a reduction in the resource allocation to the below-ground biomass and root exudation in the case of mire plants. Similarly, a decrease in the levels of malic acid and tartaric acid and an increase in the levels of succinic acid and oxalic acid in rice root exudates have been reported by Rinnan et al. (2006) in response to UV-B exposure. Recently, Zhou et al. (2022) experimentally proved that the total organic carbon (TOC) content of root exudates declines with the rise in light intensity when lettuce plants are grown under a hydroponic solution. Contrarily, increased light intensity (white light of 250 μmol m−2 s−1) significantly reduced the secretion of autotoxins (e.g., ferulic acid, benzoic acid, tannic acid, and gallic acid). Hence, the authors believe that little information is available on how and whether LED light quality impacts the root exudates of hydroponic leafy vegetables in a plant factory. Overall, the above-mentioned research strengthens the notion of the significant role of light in the root exudation process, and the authors think that there is a need to obtain a better understanding of plant exudates and soil microbiomes’ behaviour under light stress for the development of proficient and specific microbiota management strategies for agricultural sustainability.
Toxic Ions
Toxic ions, especially those of heavy metals, disturb the integrity of various cellular structures, including the cell wall and cell membrane. Toxic ions, especially in the root and leaf tissues, were reported to affect the activity of mitochondria and the cytoplasm, which further led to the generation of malondialdehyde (MDA) and reactive oxygen species (ROS) (Ghuge et al. 2023). They also influence the composition of fatty acids in plants, including linoleic acid and linolenic acid, and negatively influence membrane fluidity and function (Gill et al. 2022). It has been observed that ion toxicity disrupts the plant’s ability to take up water and essential nutrients from the soil. This can lead to water stress and nutrient deficiencies in the plant (Angulo-Bejarano et al. 2021). Ion toxicity can interfere with the plant’s transportation system, affecting the movement of sugars and nutrients from the upper to lower parts of the plant as well as posing a reduction in the chlorophyll content, negatively impacting photosystem II in chloroplasts, and resulting in decreased photosynthesis (Alengebawy et al. 2021). The presence of toxic ions in the soil can also influence the root exudation process (Vives-Peris et al. 2020). It has been noticed that aluminium toxicity in A. thaliana hosts helps in the rapid release of citrate and malate in root exudates in addition to their role in plant growth-promoting rhizobacteria recruitment (Kobayashi et al. 2013, Liu et al. 2009). The same observation has also been reported in the case of cadmium stress, which also alters the composition of root exudates in the Sedum alfredii host with the major involvement of trehalose, naphthalene, erythritol, n-octacosane, and d-pinitol in the Cd stabilisation process. Contrarily, the release of glycine, tetradecanoic acid, phosphoric acid, oxalic acid, and threonic acid is involved in the Cd mobilization process at the soil root interface (Luo et al. 2014). Recently, Jogawat et al. (2021) documented that heavy metal (e.g., Cd, Cu, and Pb) exposure leads to the production of phenolic acids, rutin, and chlorogenic acid in the case of corn plants. Comparative metabolic profiling analysis of root exudates of Sedum alfredo under Pb stress showed a significant change in the concentrations and species of root exudates, where l-proline, l-alanine, and oxalic acid help in the activation of Pb in the soil while glyceric acid and 2-hydroxyacetic acid have not much influence on Pb activation at the soil-root interface (Luo et al. 2017). Besides this, Sun et al. (2020) also documented that Cd stress showed notable alterations in the organic acids, lipids, and amino acid profiles of the root exudates of S. plumbizincicola. Further, it has also been observed that Cd stress suppresses organic acid metabolism and lipid metabolism by influencing the amino acid metabolic pathway of S. plumbizincicola under Cd stress. Leng et al. (2022) demonstrated that the phenolic root exudates have the potential to enhance the CD tolerance of Avicennia marina. They also noticed that the phenolic root exudates hinder functional bacteria-mediated iron and sulphur cycles and support Cd immobilisation in mangrove sediments. Besides this, flavonoids have also been reported to enhance Cd tolerance and offer a simulative influence on the symplasmic movement of Cd in A. marina roots (Li et al. 2015). Kidd (2001) documented the role of root exudates in Al resistance and Si-triggered amelioration of Al toxicity in three varieties (HS701b, Clavito, and Sikuani) of maize. In another independent study, Huang et al. (2023) demonstrated that amino acids and organic acids in the root exudates of Nicotiana tabacum play a crucial role in Cd uptake. However, their contribution is highly dependent on genotype type. Malate has also been reported to play a vital role in Al tolerance, and its levels in the root apex of Arabidopsis are regulated by NADP-ME1, while in its absence, the organic acid content increases. Besides this, malate is also reported to influence the signalling processes involved in the generation of reactive oxygen species (ROS) and glutamate, which are critical factors for root growth under aluminium stress (Badia et al. 2020). From the aforementioned reports, it is clear that further research is essential to get a true picture of the mechanisms operating behind root exudate secretion in response to ion toxicity at the soil-root interface.
Molecular Advances in the Understanding of the Functions of the Root Exudates
Several research publications suggested that genomics and metabolomic technologies have made a great impact and proved their relevance in crop improvement (Salem et al. 2022; Pang et al. 2021). Besides this, a noteworthy level of research has been made in the area of abiotic stress mitigation in plants, which can be accomplished by performing an in-depth analysis of the plant–microbe interactome. Different omics tools help in unzipping myriads of mechanisms associated with plant–microbe networks (Fig. 5). Hoekenga et al. (2006) also indicated that the Al-activated root malate efflux transporter (AtALMT1) performs a vital function in offering Al tolerance to the Arabidopsis host. It has been noticed that Al-toxicity in Arabidopsis thaliana hosts helps in the rapid release of citrate and malate in root exudates by activating the AtALMT1 and AtMATE1 genes, which act as Al3+ ion chelators, in addition to their role in plant growth-promoting rhizobacteria recruitment (Kobayashi et al. 2013, Liu et al. 2009). The genetic complementation studies have proved that two alleles, i.e., Ler and Col, are equally effective in providing Al tolerance and the liberation of Al-activated malate. However, it is important to mention here that although AtALMT1 is an indispensable aspect of Al tolerance, it does not contain a major QTL for Al tolerance. The Trichoderma-plant interaction (T. atroviride and T. harzianum) study performed by Tucci et al. (2011) was successful in revealing the significance of plant genotypic characters on the modulation of plant–microbe interactions, along with their influence on plant growth and stress mitigation. Similarly, Kumari et al. (2015) observed induced systemic tolerance (IST) in soybeans against salinity stress in response to the exogenous application of Pseudomonas sp. AK-1 and Bacillus sp. SJ-5 strains. Further, they also noticed that the enhancement in salt stress tolerance in plants is due to the rapid accumulation of proline and a rise in lipoxygenase activity. The role of OAs in enhancing aluminium (Al) tolerance in the roots of legume crops has been attributed to the reversal of Al-induced modifications in proteins and the metabolic regulation of plant-secreted microRNAs (Jaiswal et al. 2018). Further, it has also been observed that in the case of symbiotic rhizobia, Al tolerance is enhanced because of the production of siderophores, exopolysaccharides, activation of heavy metal-resistant efflux pumps, and up-regulation of the metal-inducible (dmeRF) gene clusters (Jaiswal et al. 2018). Zhalnina et al. (2018) used an integrated approach of comparative genomics and metabolomics to establish that the pre-programmed developmental processes in Avena barbata play an important role in defining the chemical composition of root exudates. In a similar fashion, by using an integrated microbiome and metabolomic analysis approach, Li et al. (2023) established that both drought and genotype play important roles in altering the compositions of rice rhizosphere bacterial communities and root exudates. Further, they also pointed out that suppression of amino acid exudation and organic acid exudation will serve as efficient approaches to selecting specific rhizosphere bacterial communities for rice to survive under drought stress. Wu et al. (2023) conducted a comprehensive investigation into the response of Stylosanthes guianensis (stylo) to low-phosphorus (Pi) conditions by integrating phenotypic, transcriptomic, metabolomic, and gene function analysis. They found upregulation of phosphate starvation response (PSR) genes of FLA, EXP, XTH, PAP, ABCG, and MATE families in the roots of stylo under low-Pi conditions. Further, they concluded that the PSR genes may contribute to the growth of roots, the increase in root-secreted APase activity, and the efflux of root-secreted flavonoids and organic acids in stylo, thereby promoting the recycling and utilization of soil insoluble-P and organic-P.
Research Gap, Challanges and the Way Foward
Most of the published literature indicated that phytochemical and protein secretions via roots are one of the prime and adaptive modes for plants to respond to and modify the microenvironment around roots. It has emerged from the research published over several decades that genomic and metabolic-driven technology revolutions help in generating novel information and offer a better understanding of the mechanisms or processes associated with root exudate-mediated communication among plants, soil, and other organisms dwelling in the soil. Several pieces of evidence indicate that the primary and secondary metabolites of root exudates can be employed in agricultural systems to augment crop production by activating plant defense systems against all forms of abiotic stress. However, emerging root exudation research advances in connection with serving as a strategy for plants to cope with abiotic stress indeed highlight several research gaps and crucial questions: (i) Does the composition and quantity of root exudates change under abiotic stress conditions?; (ii) How do different severity levels of abiotic stresses affect the dynamics of root exudation?; (iii) If there is a change in the root exudate profile, what are the specific compounds released by plants in response to abiotic stresses?; (v) Are these compounds known to have a role in salt stress tolerance, and do they interact with the soil environment?; (v) How does a plant regulate the production and release of root exudates in response to abiotic stresses?; (vi) Are there specific signaling pathways or molecular mechanisms involved in the plant’s ability to sense and respond to abiotic stresses stress through root exudation?; (vii) How do the altered root exudates influence the composition and function of the soil microbiome under abiotic stress conditions?; (viii) Are there specific microorganisms that are attracted or repelled by these exudates, and how does this affect the plant’s ability to cope with abiotic stresses? (ix) Do root exudates enhance nutrient availability or provide other benefits to soil microorganisms that, in turn, contribute to the plant’s abiotic stress tolerance? Answering these questions and filling the aforementioned research gap requires a multidisciplinary approach involving techniques from molecular biology, metabolomics, soil microbiology, and plant physiology. Advanced analytical methods, such as gas chromatography-mass spectrometry (GC–MS), liquid chromatography-mass spectrometry (LC–MS), capillary electrophoresis-mass spectrometry (CE-MS), Fourier transform near-infrared (FT-NIR), and nuclear magnetic resonance (NMR), could be useful for analysing the diverse classes of metabolites released by plant roots under different types of abiotic stresses. Similarly, for distinguishing plant-specific metabolites from those released by associated microbiomes, techniques such as mass spectrometry imaging (MSI), matrix-assisted laser desorption ionisation (MALDI), laser ablation electrospray ionisation (LAESI), and live single-cell mass spectrometry (LSC-MS) that offer metabolite analysis at the individual cell level will be useful, as metabolites are stable molecules and closer to the phenotype than genes and proteins. Moreover, an amalgamation of these analytical techniques may offer additional information for a comprehensive analysis of metabolites. Network analysis, machine learning, artificial intelligence, and ecological modelling can aid in establishing relationships among root exudates, microbial taxa, functional genes, and myriads of abiotic stress factors. New and innovative tools beyond metabolite-based communication, such as volatiles and small RNA, offer additional insights into the functionality and significance of root exudates at the plant-soil interface under abiotic stress conditions. Besides these, another big hurdle is the dissection and characterization of new transport systems to decode the potential regulatory mechanisms functionally involved in the process of root secretions. Genome-wide association studies (GWAS) combined with gene editing tools can identify and validate genes and metabolites influencing root exudate secretions and microbiome interactions in stressful environments. Therefore, multifaceted molecular and bioinformatic approaches should be explored to discover the interaction molecules of root exudates and rhizosphere microbiomes from a broad perspective. Besides the aforementioned points, the amalgamation of multi-omics and imaging technologies with a synthetic communities approach will be quite useful in pin-pointing and getting an accurate picture regarding the function of exudates in the interaction network between the phyllosphere (above-ground) and rhizosphere (below-ground) and vice versa. Altogether, it can be concluded that fundamental knowledge and emerging research concepts on root exudates’ behaviour, i.e., ‘cry of help’ (Rolfe et al. 2019), are necessary to have a better ecological and evolutionary understanding of the multitrophic interactions (both biotic and abiotic) for the sustainable development of agriculture. Recently, Ma et al. (2022a) have highlighted the ecological role of root exudates in promoting the rhizosphere priming effect (RPE). Root exudates contribute to RPE by directly supplying a carbon source for microorganisms and indirectly by destabilizing organic carbon. Besides direct involvement of root exudates in material cycles, they also serve as signaling agents. Signal substances such as phenols, flavins, and other secondary metabolites act as operators, establishing a communication bridge between plants and microorganisms, as well as between different plants (van der Heijden et al. 2008; Wardle 2004). Several ecological studies also reveled that plant allelopathy, replant disease, and interspecific facilitation in intercropping systems are influenced by the integrative effects of plant–microbe interactions mediated by root exudates (Zhou et al. 2023a, b; Mwendwa et al. 2021; Xu et al. 2021). For instance, root exudates of Rehmannia glutinosa are reported to stimulate the proliferation of Fusarium oxysporum. This, in turn, alters the expression patterns of Leucine-rich repeat receptor-like protein kinases, disrupts the growth and development of R. glutinosa, and leads to the formation of replant disease (Yang et al. 2023). The negative effects of replant disease in R. glutinosa are alleviated by intercropping with Achyranthes bidentata. This alleviation is attributed to the modulation of root exudates and improvement of the rhizosphere microenvironment. Li et al. (2020) investigate bacterial and fungal communities in various niches of Casuarina equisetifolia finding that ecological niche selection plays a role in shaping the assembly and diversity of microbial communities. Published literature also suggests that despite numerous initiatives to develop effective microbial consortia, there are still challenges to achieving consistent results in the field. The focus on root exudates and their functional interplay with beneficial microbes, both directly and through gene expression, will be one of the potential solutions. Therefore, researchers should emphasize the recruitment of plant growth-promoting microbes through root exudates that can enhance root colonization and establish sustainable relationships between the microbes and plants under abiotic stress conditions. This hypothesis of specific recruitment could be one of the most promising techniques to improve the performance of plant growth-promoting microbes in real-world agricultural settings.
Conclusion
Root exudates, which account for about 21% of the net photosynthates, are not a wasteful activity as their composition (quality and quantity) is regulated by crop stage, plant genotype, soil nutrient status, environmental conditions, etc. The plant adapts under adverse conditions by exuding out myriads of primary and secondary metabolites, which in turn attract a new set of microorganisms towards the root surface, facilitate nutrient cycles, or induce changes in root architecture that ultimately contribute to the higher availability of nutrients and water to the plant. The induction of root exudation by environmental conditions and their positive role in imparting abiotic stress tolerance further opens new vistas for developing new crop varieties. Root exudate profiles and exogenous use of selected exudates allow manipulation of the field conditions, which further help in supporting plant growth under abiotic stress conditions. Moreover, the recruitment of plant growth-promoting microbes through root exudates that can enhance root colonization and establish sustainable relationships between the microbes and plants under abiotic stress conditions would be a promising technique for enhancing abiotic stress tolerance in plants in real-world agricultural settings.
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The work is funded by Science and Engineering Research Board, Dept. of Science and Technology, Govt. of India via sanction order CRG/2019/000414 dated 27th January 2020.
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Ahlawat, O.P., Yadav, D., Walia, N. et al. Root Exudates and Their Significance in Abiotic Stress Amelioration in Plants: A Review. J Plant Growth Regul 43, 1736–1761 (2024). https://doi.org/10.1007/s00344-024-11237-7
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DOI: https://doi.org/10.1007/s00344-024-11237-7