Abstract
Heterotrophic prokaryotes (HP) contribute largely to dissolved organic matter (DOM) processing in the ocean, but they also release diverse organic substances. The bioavailability of DOM released by HP under varying environmental conditions has not been fully elucidated. In this study, we investigated the bioavailability of DOM released by a single bacterial strain (Sphingopyxis alaskensis) and 2 natural HP communities grown under P-replete and P-limited conditions. The released DOM (HP-DOM) was used as a substrate for natural HP communities at a coastal site in the Northwestern Mediterranean Sea. We followed changes in HP growth, enzymatic activity, diversity, and community composition together with the consumption of HP-DOM fluorescence (FDOM). HP-DOM produced under P-replete and P-limited conditions promoted significant growth in all incubations. No clear differences in HP-DOM lability released under P-repletion and P-limitation were evidenced based on the HP growth, and P-limitation was not demonstrated to decrease HP-DOM lability. However, HP-DOM supported the growth of diverse HP communities, and P-driven differences in HP-DOM quality were selected for different indicator taxa in the degrading communities. The humic-like fluorescence, commonly considered recalcitrant, was consumed during the incubations when this peak was initially dominating the FDOM pool, and this consumption coincided with higher alkaline phosphatase activity. Taken together, our findings emphasize that HP-DOM lability is dependent on both DOM quality, which is shaped by P availability, and the composition of the consumer community.
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Data Availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author upon request. Sequence data will be uploaded to the EMBL-ENA data repository.
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Acknowledgements
This work was supported by the Caramba (European Commission, H2020-MSCA-IF-2015-703991), ODISEA (Institut National des Sciences de l’Univers LEFE/CYBER 2019), and MicroPump (Agence Nationale de Recherche ANR-20-CE01-0007) projects to EOR. Flow cytometric analyses were performed at the BioPic cytometry and imaging platform (Sorbonne University/CNRS). The authors thank the crew of R/V “Nereis II” and the technicians of the Banyuls observation service for their assistance in getting Mediterranean Sea samples for the natural communities’ isolation.
Funding
This work was supported by the Caramba (European commission, H2020-MSCA-IF-2015-703991), ODISEA (INSU LEFE/CYBER 2019), and MicroPump (ANR JCJC 2020) projects to EOR.
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NB, EOR, and IO conceived the experimental work. NB, FL, CCB, LS, and EOR performed the experiments, with active contributions from OC, BM, and PC to chemical and biological analyses. NB analyzed the data with contributions from FL, CCB, LS, and EOR. NB wrote the manuscript with significant contributions from IO and EOR. All authors reviewed and approved the final manuscript.
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Bouchachi, N., Obernosterer, I., Carpaneto Bastos, C. et al. Effects of Phosphorus Limitation on the Bioavailability of DOM Released by Marine Heterotrophic Prokaryotes. Microb Ecol 86, 1961–1971 (2023). https://doi.org/10.1007/s00248-023-02201-1
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DOI: https://doi.org/10.1007/s00248-023-02201-1