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Serious Risk of Tigecycline Resistance in Escherichia coli Isolated from Swine Manure

  • Environmental Microbiology
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Abstract

The emergence of the plasmid-mediated tigecycline resistance gene tetX family in pig farms has attracted worldwide attention. The use of tetracycline antibiotics in pig farms has a facilitating effect on the prevalence of the tetX family, but the relationship among its presence, expression, and resistance phenotype in resistant bacteria is unknown. In this study, the presence and expression characteristics of tetracycline resistance genes (TRGs) in 89 strains of doxycycline-resistant E. coli (DRE) isolated from pig manure samples from 20 pig farms under low concentrations of doxycycline stress (2 μg/mL) were analyzed. The detection rate of tetO was 96.63%, which is higher than those of other TRGs, such as tetA (94.38%), tetX (76.40%), tetB (73.03%), and tet(X4) (69.66%). At least three TRG types were present in DRE strains, which thus showed extensive resistance to tetracycline antibiotics, and 37% of these strains were resistant to tigecycline. In the presence of a low concentration of doxycycline, tetA played an important role, and the expression and existence ratio of TRGs indicated low expression of TRGs. Furthermore, the doxycycline resistance of DRE was jointly determined by the total absolute abundance of TRGs, and the absolute abundance of tetX and tet(X4) was significantly positively associated with tigecycline resistance in DRE (P < 0.05). Overall, DRE isolated from swine manure is an important reservoir of the tetX family, which suggests that DRE in swine manure has a high risk of tigecycline resistance, poses a potential threat to human health, and should be of public concern.

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Funding

This work was supported by the National Natural Science Foundation of China (grant no. 32172781, 31872401), the Laboratory of Lingnan Modern Agriculture Project (grant no. NZ2021027), the Project of Swine Innovation Team in Guangdong Modern Agricultural Research System (grant no. 2022KJ126), the Start-up Research Project of Maoming Laboratory (grant no. 2021TDQD002), the Science and Technology Program of Guangdong Province, China (grant no. 2020B1212060060), and the Research and Development Project of Shenzhen Kingkey Smart Agriculture Times Co., Ltd. (grant no. TLJXN-FWJS20200615001).

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Authors and Affiliations

  1. Guangdong Laboratory for Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China

    Tao Chen, Minxing Zhao, Xiaoyue Tang, Wenqiang Wang, Miao Zhang, Jing Tang, Wei Wang, Wenxiao Wei, Jiandui Mi, Yan Wang, Xindi Liao & Yinbao Wu

  2. Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, 525000, China

    Jiandui Mi, Yan Wang, Xindi Liao & Yinbao Wu

  3. National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, China

    Jiandui Mi, Yan Wang, Xindi Liao & Yinbao Wu

  4. Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China

    Jiandui Mi, Yan Wang, Xindi Liao & Yinbao Wu

  5. Guangdong Engineering Technology Research Center of Harmless Treatment and Resource Utilization of Livestock Waste, Yunfu, Xinxing, China

    Jiandui Mi, Yan Wang, Xindi Liao & Yinbao Wu

  6. Ministry of Agriculture Key Laboratory of Tropical Agricultural Environment, South China Agricultural University, Guangzhou, 510642, China

    Jiandui Mi, Yan Wang, Xindi Liao & Yinbao Wu

  7. Foshan Customs Comprehensive Technology Center, 528200, Foshan, China

    Baohua Ma, Yongde Zou & Na Zhang

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  1. Tao Chen
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Writing—original draft preparation, conceptualization, and visualization: Tao Chen; methodology: Minxing Zhao, Xiaoyue Tang; formal analysis and investigation: Wenqiang Wang, Miao Zhang, Jing Tang, Wei Wang, Wenxiao Wei; resources: Baohua Ma, Yongde Zou, Na Zhang; supervision: Jiandui Mi, Yan Wang, Xindi Liao; project administration and funding acquisition: Yinbao Wu.

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Chen, T., Zhao, M., Tang, X. et al. Serious Risk of Tigecycline Resistance in Escherichia coli Isolated from Swine Manure. Microb Ecol 86, 947–958 (2023). https://doi.org/10.1007/s00248-022-02133-2

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