Abstract
Aims/hypothesis
Diabetes is associated with an increased risk of atherosclerotic cardiovascular disease, but whether there is a direct and independent role for impaired glucose control in atherogenesis remains uncertain. We investigated whether diabetes with poor glycaemic control would accelerate atherogenesis in a novel pig model of atherosclerosis, the D374Y-PCSK9 + transgenic minipig.
Methods
Nineteen minipigs were fed a cholesterol-enriched, high-fat diet; ten of these pigs were injected with streptozotocin to generate a model of diabetes. Restricted feeding was implemented to control the pigs’ weight gain and cholesterol intake. After 49 weeks of high-fat feeding, the major arteries were harvested for a detailed analysis of the plaque burden and histological plaque type.
Results
Stable hyperglycaemia was achieved in the diabetic minipigs, while the plasma total and LDL-cholesterol and creatinine levels were unaffected. Diabetes failed to increase atherosclerosis in any of the vessels examined. The plaque burden in the aorta and right coronary artery was comparable between the groups, and was even reduced in the left anterior descending (LAD) coronary and iliofemoral arteries in the diabetic pigs compared with the controls. The distribution of plaque types and the collagen and macrophage contents were similar between the groups, except for a reduced infiltration of macrophages in the LAD arteries of the diabetic pigs.
Conclusions/interpretation
Poorly controlled diabetes with no alterations in plasma cholesterol or creatinine concentrations did not augment the plaque burden or promote the development of more advanced lesions in this large-animal model of human-like atherosclerosis. This is consistent with clinical studies in patients with type 1 diabetes, indicating that hyperglycaemia per se is not an independent promoter of atherosclerotic disease, but that other diabetes-associated risk factors are important.
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Introduction
Type 1 diabetes is associated with a substantially increased risk of atherosclerotic cardiovascular disease and cardiovascular mortality [1–5]. Several factors may explain this excess risk, including hyperglycaemia, renal dysfunction, hypertension and dyslipidaemia, but the potential impact of each factor and the evidence for the underlying mechanism remain disputed [1, 6, 7]. The association between diabetic hyperglycaemia and other diabetes-associated risk factors, for example kidney damage [8, 9], complicates the analysis of human data. The direct effect of poor glycaemic control on atherogenesis is thus subject to debate, with some studies [10–12] pointing to hyperglycaemia as an independent predictor of subclinical and clinical atherosclerosis in diabetic patients, but others [3, 13] failing to detect such an association, especially after controlling for competing risk factors.
Animal experiments may suffer from similar limitations. In most studies of wild-type and genetically modified mice used as a model of diabetes, type 1 diabetes has been reported to boost plasma cholesterol concentrations [14–21], making it difficult to isolate the effect of glucose dysregulation on atherogenesis. Similarly, diabetes has been reported to augment cholesterol levels in Yucatan and Sinclair minipigs [22, 23]. In the widely used Yorkshire pig model of diabetes, inconsistent results have been obtained regarding the effect of diabetes on atherosclerosis and cholesterol level [24–26]. Impairment of kidney function and severe weight faltering in animal models of diabetes may further obscure the link between hyperglycaemia and atherosclerosis.
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a protein that binds to the LDL receptor and facilitates its degradation in hepatocytes. By overexpressing a hyperactive (D374Y) human form of PCSK9 in the hepatocytes of Yucatan minipigs (D374Y-PCSK9 + minipigs), we recently created a model of hypercholesterolaemia and accelerated human-like atherosclerosis [27]. In the current study, we present methods for controlled induction and management of hyperglycaemia in D374Y-PCSK9 + minipigs, to generate a model of type 1 diabetes. Furthermore, we report the effect of impaired glycaemic control on the development of atherosclerosis in the absence of diabetes-associated changes in plasma cholesterol or creatinine concentrations, and while maintaining steady body growth.
Methods
Animals
Yucatan minipigs were obtained from breeding colonies maintained at Aarhus University and housed in specific pathogen-free stable facilities. Transgenic minipigs were bred by mating wild-type sows with two cloned, genetically identical boars harbouring transgene insertions on chromosomes X and 9 [27]. Male transgenic offspring with the chromosome 9 transgene and females offspring with the chromosome X transgene were randomly assigned to either a control group (four females and five males) or a diabetes model group (DM; five females and seven males). The animals in the control group were also part of a concurrent study that was recently published [27] in which the phenotype was described in detail. A high-fat, high-cholesterol (HFHC) diet was mixed by adding 20% (wt/wt) of lard and 2% cholesterol (Sigma-Aldrich, Brøndby, Denmark) to standard pig chow. All the pigs were started on the HFHC diet at 8–9 weeks of age and fed ad libitum until they reached a weight of 20 kg, after which feeding was restricted to 700 g divided into two daily portions, which were completely consumed by the pigs without wastage. Restricted feeding was implemented to control the pigs’ weight gain and to ensure an equal cholesterol intake in all the animals. The principles of laboratory animal care (NIH publication no. 85–23) were followed. The Danish Animal Experiments Inspectorate approved all the experimental procedures.
Induction of diabetes
Doses of streptozotocin (STZ; Sigma-Aldrich) were calculated based on body weight (110 mg/kg), and the first injection was carried out 2–4 weeks after the start of the HFHC diet. Prior to STZ injection, animals in the DM group were fasted for 16 h and sedated as detailed in the electronic supplementary material (ESM) Methods. STZ (100 mmol/l) was dissolved in sodium citrate buffer (100 mmol/l, pH 4.5) as previously described [24] and immediately injected over 2–3 min through sterile filters into an ear vein.
Fasting plasma glucose (FPG) in the DM group of pigs was monitored twice a week using a hand-held glucometer (Accu-Check Compact Plus; Roche, Basel, Switzerland). If the average FPG dropped below 10 mmol/l for 2 consecutive weeks, the STZ injection was repeated. Insulin treatment (isophane insulin; Novo Nordisk, Bagsværd, Denmark) was initiated if the FPG increased to above 20 mmol/l on two successive measurements within a week, and was continued until the animals were killed. Insulin was injected subcutaneously once daily, and the dose was adjusted to keep the FPG at 12–17 mmol/l as detailed in ESM Table 1. When the animals received insulin, the FPG was measured immediately prior to that day’s insulin administration, i.e. 24 h after the last insulin administration. One DM pig (male) was killed after the development of plasma acidosis and ketonuria. Another DM pig (female) was killed due to the development of a cataract. Thus, in the DM group, ten pigs (four females, six males) completed the study. Fasting blood samples were drawn from the jugular vein at the time points indicated. For details on plasma analysis, see the ESM Methods.
Tissue analysis
The control pigs were killed 46 (range 43–48) weeks and the diabetic pigs 49 (range 42–51) weeks after initiation of the HFHC diet. The procedures for killing the animals and analysing the tissues were followed as previously described [27]. In brief, after the pigs had been sedated, a lethal dose of pentobarbital (Lundbeck, Copenhagen, Denmark) was injected and the heart and major arteries were extracted. All the tissues were immersion-fixed in 4% formaldehyde for 6 h. The aorta, right iliofemoral artery (proximal 10 cm) and right coronary artery (RCA; proximal 6 cm) were stained en face through immersion in Sudan IV solution (Sigma-Aldrich; 5 g/l in 96% ethanol) for 5 min, followed by 90 s of wash-out in 96% ethanol. Digital images of the stained intimal surfaces were analysed with custom-made computer software.
The proximal 1.5 cm of the left anterior descending (LAD) coronary artery was cross-sectioned at 3 mm intervals. The largest raised plaque in the abdominal aorta, typically situated within 3 cm of the renal arteries, was identified by macroscopic inspection and excised for histological analysis. A longitudinal slice from the aortic root to the branching point of the upper body vasculature was obtained from the major curvature of the ascending aorta. The aortic and coronary slices were embedded in paraffin, sectioned (3 μm sections) and stained with haematoxylin and eosin using routine methods. The intimal and medial areas of the coronary sections were quantified using ImageJ software (National Institutes of Health, Bethesda, MD, USA) and the mean values were calculated for each vessel. The classification of histological lesion type proposed by Virmani et al [28] was performed in a blind approach by two observers, with intra- and inter-observer Cohen’s weighted κ values of 0.86 and 0.79, respectively. In the LAD arteries, the most advanced plaque was selected for a between-groups comparison of plaque type.
Plaque macrophages were detected by immunohistochemical staining using rabbit anti-muramidase primary antibody (1:500, A0099; Dako, Glostrup, Denmark), biotinylated secondary antibody (E0453; Dako), alkaline phosphatase-conjugated streptavidin (SA-5100; Vector Labs, CA, USA) and Liquid Permanent Red (K0640; Dako). To quantify the collagen, sections were stained with Sirius Red and analysed under polarised light. The stained areas were measured in ImageJ using the Threshold tool.
Statistical analysis
Statistical analyses were performed using Prism software (GraphPad Software, La Jolla, CA, USA). Normally distributed data are presented as means ± SEM and non-normal data are displayed as the median and interquartile range (IQR). Group comparisons were made using an unpaired Student’s t test, Welch’s t test or Mann–Whitney U test as appropriate. Differences in time series measurements were assessed by comparing the AUCs. Plaque types were grouped into early lesions (intimal thickening or xanthomas) and progressive lesions (pathological intimal thickening or fibroatheromas) in contingency tables, and Fisher’s exact test was used to assess the differences. The data were initially analysed separately for each sex, but no sex differences were detected for any of the measures of atherosclerosis or plasma metabolites. Therefore the data for male and female pigs were combined to increase the statistical power. The significance level was set to <0.05 and p values are presented for significant results.
Results
Induction of diabetes
The level of hyperglycaemia following the first injection of STZ varied, which is a known issue with standard weight-adjusted dose administration regimens. However, by using an algorithm with repeated STZ injections (1–4 injections, median 2.5 injections per pig), diabetes was achieved in all the pigs. A stable FPG >10 mmol/l was reached at a median of 9 (IQR 2–12) weeks after the start of the HFHC diet. Insulin therapy was initiated at a median of 19 (IQR 18–23) weeks after the start of the HFHC diet, which restricted the FPG to below 20 mmol/l (Fig. 1a). The pigs injected with STZ showed a significantly higher cumulative FPG load (a 3.3-fold increase in the AUC) compared with the control pigs (p < 0.0001), in which the FPG remained stable at approximately 5 mmol/l.
Due to the limited transmembrane glucose permeability of porcine erythrocytes, fructosamine rather than glycated haemoglobin reflects long-term glycaemic levels in pigs [29]. Fructosamine levels paralleled glucose levels after the STZ injections in the DM pigs (Fig. 1b). Insulin therapy triggered a transient decline in fructosamine levels, but the high levels were re-established after adjustment of the insulin doses. The AUC for fructosamine correlated well with the AUC for FPG prior to insulin administration (p < 0.0001, r 2 = 0.95) and during insulin therapy (p < 0.0001, r 2 = 0.96) (ESM Fig. 1a). The AUC for fructosamine was higher in the diabetic pigs than in the controls (p < 0.0001), and all the DM pigs achieved a fructosamine AUC of at least 6 SD above the mean of the control group (Fig. 1c). The fructosamine measurements thus further confirmed that increased glycaemic load was successfully achieved in all the STZ-treated animals.
Levels of C-peptide, which is derived from endogenous insulin production, initially declined on the HFHC diet for the pigs in both groups, but it was maintained at much lower levels in the STZ-injected than in the control pigs (p < 0.005 for difference in AUC). In the DM group, several pigs had C-peptide levels towards the end of study that were the same as or lower than the reported detection limit of the assay (0.033 nmol/l) (Fig. 1d).
The body weights were not significantly different between the two groups at the start of the experiment, and both groups gained weight steadily, although the DM pigs trailed 11% behind the control group at the end of the experiment (p < 0.05; Fig. 1e). No differences were detected in renal (creatinine) or hepatic (alanine aminotransferase) markers (Fig. 1f and ESM Fig. 1b).
Effects on plasma lipids
Levels of total cholesterol, LDL-cholesterol and HDL-cholesterol increased in all the animals following the initiation of the HFHC diet. The increase in HDL-cholesterol was modest and ceased after 4 weeks, whereas total cholesterol and LDL-cholesterol concentrations continued to rise, reaching approximately 20 mmol/l and approximately 12 mmol/l, respectively (Fig. 2a–c). No differences were detected between the groups, indicating that diabetes did not modulate the cholesterol levels. Size exclusion chromatography of the pooled plasma samples was consistent with these measurements and further revealed a steady increase in the cholesterol carried by VLDL-sized particles in both groups (Fig. 2d–f). The lipoproteins were of similar size, but the fraction of cholesterol carried by the VLDL-sized particles was lower in the DM group at week 20 compared with the control animals. Plasma levels of human PCSK9 were similar in both groups (ESM Fig. 1c).
Baseline triacylglycerol levels were higher in the DM group compared with the control group, but this was mainly driven by two data points and was not statistically significant. During the study, the triacylglycerol levels were higher on average in the diabetic group (p < 0.001 for AUC; Fig. 3a). This link between diabetes and increased plasma triacylglycerol concentrations was further corroborated by a significant correlation between the AUC for triacylglycerol and the AUC for fructosamine (exponential growth correlation, r 2 = 0.83, p < 0.0005; Fig. 3b). Size fractioning revealed, as expected, that triacylglycerol was mainly stored in VLDL-sized particles (Fig. 3c–e).
Effects on atherosclerosis
Atherosclerotic lesions in the thoracic aorta of all the pigs were predominantly confined to the aortic root and arch. Raised lesions were found solely in the abdominal part. The areas of the atherosclerotic lesions in the aorta expressed as a percentage of the total surface area were 40.3% (±4.7% SEM) in the control pigs and 30.9% (±2.8% SEM) in the diabetic pigs, and the difference was not significant (Fig. 4a). Lesions in the iliofemoral arteries were more extensive in control pigs (47.2% ± 11.4% SEM) than diabetic pigs (10.7 ± 2.4% SEM, p < 0.05; Fig. 4b). En face analysis revealed no significant differences in the coverage of RCA lesions (Fig. 4c), but there was a significant decrease in the absolute cross-sectional area of the lesions in the LAD arteries of the diabetic pigs (p < 0.05; Fig. 4d). The latter result may be influenced by a smaller LAD artery in diabetic pigs, as measured by a 19% reduction in the area of the media (p < 0.05; ESM Fig. 2a); this may reflect, at least in part, the lower body weight. The ratio of intima/media area in the LAD arteries was not significantly different between the groups (ESM Fig. 2b).
Group-wise correlation analysis revealed that neither body weight (data not shown) nor the total dose of injected insulin (ESM Fig. 3) in the DM pigs correlated significantly with the atherosclerotic burden in any of the vessels examined.
The classification of LAD artery, thoracic aortic and abdominal aortic lesions, based on the scheme proposed by Virmani et al [28], revealed that the lesions were mostly pathological intimal thickenings in the LAD arteries and thoracic aorta, but the abdominal aorta contained more fibroatheromas (Fig. 5a–f). Importantly, no significant group differences in the distribution of lesion types were detected (Fig. 5g–i). No differences in collagen content, measured as the percentage of the area stained with Sirius Red, were detected in the vessels examined (Fig. 6d–f). The macrophage content, measured as the percentage of the area that was muramidase positive, was reduced in LAD artery lesions in diabetic pigs compared with controls, consistent with the smaller lesion size, but otherwise no differences in macrophage content were detected (Fig. 6g–i).
Discussion
In the current study, we established a protocol for the induction and management of diabetes in a novel atherosclerosis-prone pig model. In contrast to many previous studies, we found no effects of diabetes on the level of total or LDL-cholesterol, and no signs of kidney function impairment. We conducted analyses of atherosclerosis in several clinically relevant vascular beds and found no aggravating effect of impaired glucose control on the amount or type of atherosclerotic lesions. On the contrary, diabetes was associated with a reduced burden of atherosclerosis in the proximal LAD and iliofemoral arteries.
Animal models of diabetes and atherosclerosis
Animal models potentially hold the key to testing the isolated effect of hyperglycaemia on atherogenesis, but animal models that combine type 1 diabetes and atherosclerosis have generally suffered from concurrent effects on plasma cholesterol.
In mice of various genetic backgrounds, type 1 diabetes has been reported to increase atherosclerosis, but this has often been accompanied by a marked diabetes-induced elevation in plasma cholesterol concentrations [14–21]. When cholesterol levels were not significantly altered by diabetes, inconsistent results were observed and atherosclerosis was augmented [20, 30], unmodified [21, 31, 32] or even reduced [33]. Interestingly, in one study, when diabetic and control mice were matched based on cholesterol levels, the initially significant difference between the two groups disappeared [20].
Pigs provide models of atherosclerosis that are close to the human pathology with respect to the size and composition of the plaques, and unlike mice, the disease is initiated in an arterial intima that already contains various amounts of cells and extracellular matrix [34]. This is particularly relevant for studies on diabetes because the alteration in the composition of the arterial matrix that is seen in diabetes has been hypothesised to increase the susceptibility to atherosclerotic lesion formation [35]. Relatively few studies have examined the atherogenic effect of hyperglycaemia in pigs (Table 1). In a small study of wild-type Yucatan minipigs in which they examined one section of 0.75–2 mm from each vessel, Wang et al detected an increase in intima/media thickness in two out of six vascular beds examined [22]. Dixon et al found increased sudanophilia in the carotid artery of diabetic Sinclair minipigs [23]. In both studies, however, the results were potentially confounded by a near doubling of LDL-cholesterol concentration [22, 23]. Dixon et al also examined Yucatan minipigs and reported vascular changes on intravascular ultrasound (a raised endothelium) in a higher number of diabetic coronary segments compared with controls, but it is not known whether this represented atherosclerosis because tissue analysis, including histology, was not performed [36].
In a seminal paper, Gerrity et al showed increased aortic atherosclerosis and coronary luminal stenosis in diabetic Yorkshire pigs as early as 20 weeks into the study, without concomitant changes in total cholesterol levels [24]. However, in two subsequent studies in Yorkshire × Landrace and Yorkshire pigs, respectively, diabetes was accompanied by augmented hypercholesterolaemia. In the first of these studies, van den Heuvel et al reported increased atherosclerosis in abdominal aortas [26], but in the other, Hamamdzic et al observed no effect on intimal area, intima/media ratio or the degree of stenosis of the coronary arteries, although the lesions were reported to be more complex in diabetic compared with control animals [25]. In the latter study, an approximately 50% reduction in body weight was also reported for the diabetic pigs compared with the controls.
Since combined insulin deficiency and hyperglycaemia has been documented in all of the reports in pigs (Table 1) and mice [14–21, 30–33], the failure to consistently augment atherosclerosis suggests that impaired glycaemic control per se may not accelerate atherogenesis, but that other factors that are variably present in diabetic animal models, including elevated cholesterol and kidney damage, may play an important role. The lack of an atherogenic effect of diabetes in this study supports this interpretation.
Effect of diabetes on plasma lipids
The lack of diabetes-associated hypercholesterolaemia in D374Y-PCSK9 + transgenic minipigs is an important strength compared with many previous animal studies. Insulin has been shown to upregulate the expression of the LDL receptor [37], and reduced hepatic LDL uptake due to insulin deficiency may be one mechanism underlying hypercholesterolaemia in diabetes [38]. Recent work in mice has shown that the restriction of PCSK9 expression by insulin plays a major role in mediating this effect [38]. In our transgenic minipigs, human D374Y-PCSK9 is expressed under a constitutively active liver-specific promoter [27] rather than the native insulin-controlled PCSK9 promoter. Furthermore, the high expression level of D374Y-PCSK9 overwhelms the effect of any changes in endogenous porcine PCSK9. Thus, the regulatory link between insulin and LDL receptor levels may be effectively disrupted, which may explain the lack of diabetes-induced hypercholesterolaemia in D374Y-PCSK9 + minipigs. A reduced binding of apoB48-containing remnants to hepatic proteoglycans has been suggested to cause cholesterol accumulation in diabetic LDL-receptor-deficient mice [39], but this mechanism is probably of less relevance in pigs (or humans), where the bulk of the cholesterol is carried in apoB100-containing LDL/VLDL particles. Furthermore, diabetic hyperphagia [39, 40], with an increased dietary cholesterol intake in animals fed ad libitum, has been shown to contribute to diabetic hypercholesterolaemia [41, 42]. To counteract this effect, we secured a uniform intake of the HFHC diet by using a restricted feeding regimen.
An increased AUC for triacylglycerol was seen in the diabetic group, which is consistent with observations in type 1 diabetic patients, but, importantly, our diabetic pigs did not develop the kinds of giant lipoproteins that have previously been found to reduce atherogenesis in diabetic rabbits [43].
Hyperglycaemia and coronary heart disease
Although studies have reported that individuals with type 1 diabetes suffer from an increased risk of atherosclerosis [3, 4] and cardiovascular mortality [1, 2], the direct and independent contribution of glycaemic dysregulation to this excess risk remains to be proven. Table 2 provides a summary of human studies that have been conducted on major cohorts of patients with type 1 diabetes in recent years. As depicted, the studies are not unanimous in identifying hyperglycaemia as an independent predictor of subclinical atherosclerosis or ischaemic coronary events when competing risk factors are controlled for.
The Wisconsin Epidemiologic Study of Diabetic Retinopathy [44] and two follow-up studies on patients from the DCCT [10, 11, 45] all identified hyperglycaemia as an independent predictor of atherosclerotic cardiovascular endpoints. In contrast, the EURODIAB study showed that the initially significant link between hyperglycaemia and coronary heart disease was rendered insignificant once albuminuria and other candidate risk factors were included in the analysis [13]. Furthermore, while studies may adjust for multiple risk factors at baseline, competing effects of changes in risk factors over the study period, for example incident renal damage and long-term cholesterol levels, are often neglected. Such incompletely adjusted associations may overestimate the role of hyperglycaemia in human studies in light of the positive correlations seen between hyperglycaemia and other candidate causal agents, for example dyslipidaemia and the development of kidney disease [8, 9].
Our results suggest that hyperglycaemia due to insulin deficiency per se is not a promoter of atherosclerosis, and support alternative interpretations of the association between diabetes and atherosclerotic heart disease in humans. Although an investigation of type 2 diabetes is beyond the scope of this work, it is worth noting that glycaemic control in patients with type 2 diabetes has repeatedly failed to provide protection against atherosclerotic cardiovascular disease in randomised clinical trials [46, 47].
Strengths and limitations
The large-animal model used in this study includes the development of atherosclerotic lesions with many similarities to those seen in humans [27]. Porcine vessels contain a pre-existing intima, and lesions develop that show rich fibrous tissue, macrophage infiltration, necrosis and calcification. It was also possible to induce insulin deficiency and maintain severe hyperglycaemia during exogenous insulin therapy, thus replicating poor glycaemic control in diabetic humans. Importantly, this could be achieved without the diabetes-induced increases in cholesterol levels that are seen in other animal studies, presumably because of the clamped high level of PCSK9. An important limitation of the model, which it has in common with previous animal studies of diabetes and atherosclerosis, is that the lesions do not develop to the stage that causes thrombotic complications or stable stenosis in humans. Therefore, our study cannot disregard potential effects of diabetes on late-stage atherosclerosis, plaque vulnerability or arterial remodelling. Furthermore, potential effects of diabetes acting through changes in lipoprotein metabolism, including, in particular, changes in the distribution of lipoprotein sizes, may not be accurately addressed in porcine models because of the lack of cholesteryl ester transfer protein.
Conclusion
In conclusion, we have established a model of chemically induced diabetes in D374Y-PCSK9 + transgenic minipigs that is free of important factors that may obscure the link between impaired glucose control and atherosclerosis. We found that diabetes did not accelerate the extent of the atherosclerosis or promote complexity of the lesions. This supports the need to focus on diabetes-associated factors beyond hyperglycaemia to understand the causes of accelerated atherosclerotic cardiovascular disease in patients with type 1 diabetes.
Abbreviations
- DM:
-
Diabetes model group
- FPG:
-
Fasting plasma glucose
- HFHC:
-
High-fat high-cholesterol
- IQR:
-
Interquartile range
- LAD:
-
Left anterior descending
- PCSK9:
-
Proprotein convertase subtilisin/kexin type 9
- RCA:
-
Right coronary artery
- STZ:
-
Streptozotocin
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Acknowledgements
We thank A. M. Toft, K. P. Jensen (AU-Foulum, Aarhus University, Denmark) and the rest of the personnel at AU-Foulum’s stable facilities for the blood sampling, insulin injection and practical handling of the pigs; K. D. Winther (AU-Foulum, Aarhus University, Denmark) for veterinary consultations; and Z. P. Nasr, D. W. Qualmann and L. M. Røge (Department of Clinical Medicine, Aarhus University, Denmark) and C. Berthelsen (AU-Foulum, Aarhus University, Denmark) for providing excellent technical assistance.
Funding
Funding was received from the Danish Council for Independent Research|Medical Sciences, Lundbeck Foundation, Danish Heart Foundation, Aase og Ejnar Danielsen Foundation, Snedkermester Sophus Jacobsen og hustru Astrid Jacobsens Foundation and Murermester Lauritz Peter Christensen og hustru Kirsten Sigrid Christensens Foundation.
Duality of interest
The authors declare that there is no duality of interest associated with this manuscript.
Contribution statement
EF and JFB conceived the study. RHA, EF and JFB planned and performed the induction of diabetes. RHA organised the blood sampling, insulin treatment and other practical aspects. RHA, CC and TL acquired the plasma data and undertook the subsequent chemical analysis and interpretation. RHA, MMB, MBM and JFB killed the animals and undertook the tissue data extraction and subsequent pathological analysis and interpretation. RHA performed the final data analysis. JFB supervised the experiments. RHA, EF and JFB wrote the manuscript. All authors critically revised the manuscript and approved the final version. RHA is the guarantor of this work and, as such, had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis.
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Al-Mashhadi, R.H., Bjørklund, M.M., Mortensen, M.B. et al. Diabetes with poor glycaemic control does not promote atherosclerosis in genetically modified hypercholesterolaemic minipigs. Diabetologia 58, 1926–1936 (2015). https://doi.org/10.1007/s00125-015-3637-1
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DOI: https://doi.org/10.1007/s00125-015-3637-1