Abstract
Key message
All East African Highland Banana varieties are genetically uniform having arisen from a single clone introduced to Africa.
Abstract
East African Highland bananas (EAHBs) are a subgroup of triploid (AAA genome) bananas of importance to food security in the Great Lakes region of Africa. Little is known about their genetic variation, population structure and evolutionary history. Ninety phenotypically diverse EAHB cultivars were genotyped at 100 SSR microsatellite markers to investigate population genetic diversity, the correlation of genetic variability with morphological classes, and evolutionary origins since introduction to Africa. Population-level statistics were compared to those for plantain (AAB) and dessert (AAA) cultivars representing other M. acuminata subgroups. EAHBs displayed minimal genetic variation and are largely genetically uniform, irrespective of whether they were derived from the distinct Ugandan or Kenyan germplasm collections. No association was observed between EAHB genetic diversity and currently employed morphological taxonomic systems for EAHB germplasm. Population size dynamics indicated that triploid EAHBs arose as a single hybridization event, which generated a genetic bottleneck during foundation of the EAHB genepool. As EAHB triploids are sterile, subsequent asexual vegetative propagation of EAHBs allowed a recent rapid expansion in population size. This provided a basis for emergence of genetically near-isogenic somatic mutants selected across farmers and environments in East Africa over the past 2000 years since EAHBs were first introduced to the African continent.
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Introduction
Bananas (genus Musa, family Musaceae) are monocotyledons from the Zingiberales, a sister group of the Poales and of major economic importance (e.g., food security and income) in many tropical and subtropical countries (Martin et al. 2013). Though not well understood, the Musa domestication process is considered to have started some 7000 years ago in Southeast Asia (D’Hont et al. 2012). It is speculated that spontaneous hybridization and triploidization events involving almost all diploid cultivars lead to the formation of cultivated triploids, including pure M. acuminata varieties (AAA) and interspecific M. acuminata × M. balbisiana varieties (AAB, ABB) (Till et al. 2010; Perrier et al. 2011; Pachuau et al. 2014). Indeed, it is likely that sterility and parthenocarpy could have been selected for in triploids to generate seedless fruits with increased pulp mass, which gave rise to the current predominantly sterile triploid cultivars (Perrier et al. 2009, 2011; de Jesus et al. 2013).
The East African Highland bananas (EAHB; also known as the ‘Lujugira-Mutika’ subgroup) have a triploid A genome (AAA). The EAHBs consist of many farmer-selected landrace cultivars that dominate the Great Lakes region of East Africa (Karamura 1998) with no clear analogs globally (Pillay et al. 2001). The current diversity of this subgroup is believed to have resulted from the accumulation of somatic mutations that consistently recur in the crop—a condition that is still not well understood (De langhe 1961, 1964; Crane and Laurence 1956; Shepherd 1957; Ude et al. 2003). Based on the phenotypic diversity of the EAHBs, the Great Lakes region is considered as a secondary center of banana diversity (Tugume et al. 2003).
The edible diploid M. acuminata subspecies zebrina and banksii are considered to have a recent common ancestry with EAHB (Li et al. 2013). The M. acuminata subspecies banksii is also related to the AAB Plantains, which have a “B” genome ancestry from M. balbisiana (Perrier et al. 2011). Lack of historical records and archeological evidence has limited understanding of the movement of the EAHB genepool outwards from their center of origin (South East Papua New Guinea). However, the geographical ranges of extant Musa acuminata diploid parents and triploid hybrids support a scenario of numerous ancient migrations both before (in diploids) and after hybridization (in triploids). Current evidence suggests a single introduction of AA or AAA varieties ‘Lujugira-Mutika’ into Africa about 2500 years ago, which was distinct from introduction(s) contributing to the origins of AAB Plantains (Perrier et al. 2011).
Like most cultivated bananas, the EAHBs are strictly vegetatively propagated via suckers planted by farmers. The absence of seed propagation involving sexual recombination has resulted in cultivated bananas having a limited potential for producing genetically diverse offspring. Yet, morphological variation does exist within the EAHB subgroup which is typically considered to have arisen through the accumulation of somaclonal mutations leading to somaclonal variants (Perrier et al. 2011). In this regard, approximately 120 EAHB cultivars are recognized across Uganda, Kenya, western Tanzania, Rwanda, Burundi and eastern Congo (Karamura 1998; Ssebuliba et al. 2005), although about 200 different names are used as a number of local names may exist for the same cultivar.
Even though the morphotaxonomic system has provided a coherent classification basis for this banana subgroup (Simmonds and Shepherd 1955; Shepherd 1957; Karamura 1998), this system has inadequate resolution for populations within species (Christelova et al. 2011). Many EAHB cultivars cannot be easily distinguished on the basis of their morphology, especially if they are closely related (Pillay et al. 2001). Such morphological diversity measures are often inaccurate (Abdullah et al. 2012), where they can under- or over-estimate the extent of genetic variability (Resmi et al. 2011). An improved understanding of molecular genetic diversity of extant EAHB population can enable more informed and improved approaches to EAHB breeding (Ganapathy et al. 2012).
Pillay et al. (2001) used RAPDs as molecular markers to analyze the genetic diversity of 29 EAHBs and concluded that, while they could be distinguished, they were closely related with a narrow genetic base (Cooper et al. 2000). Using AFLP, Tugume et al. (2003) reported low levels of DNA diversity of 115 EAHB cultivars and his molecular classification somehow matched the morphological characterization of Karamura (1998). Other published studies have included EAHB cultivars to represent out-group taxa or as a genomic group (AAA) and have corroborated the above findings (Ude et al. 2003; Christelova et al. 2011; Changadeya et al. 2012; Hippolyte et al. 2012; de Jesus et al. 2013). However, contradictory results regarding the diversity of the EAHB have also been reported based on 24 SSR markers (Buwa 2009).
While morphological variation exists between the different EAHB varieties, many such varieties have the potential to be genetically very similar, particularly if such varieties have arisen via accumulation of somatic mutations. Given that EAHBs are a starchy staple food for over 80 million people in the Great Lakes region of Africa, the potential genetic vulnerability of EAHBs (e.g., to neo-virulent diseases and changing environmental pressures) due to a narrow genetic base is a major consideration (Cooper et al. 2000). In addition, the complex and lengthy breeding cycles associated with EAHBs pose a challenge for broadening the genetic base of EAHBs (Li et al. 2013).
Of major concern is the genetic vulnerability of bananas to Fusarium wilt (or panama) disease caused by strains of a soil fungus F. oxysporum cubense (Foc). The original Foc strain of Fusarium wilt previously eliminated the Gros Michel banana cultivar, the most common export banana from the nineteenth century until the 1950s. Gros Michel was replaced with a Cavendish variety, which was resistant to the original Foc strain. However, the Cavendish is susceptible to a new Foc Tropical Race 4 (Foc-TR4) strain, and is likely to succumb in the same manner as the Gros Michel variety. The Foc-TR4 variant was first detected in Southeast Asia in the 1990s and has since been spreading to other banana growing regions. More recently, the Foc-TR4 variant has been reported in Jordan (García-Bastidas et al. 2014) and Mozambique (Butler 2013), where its continuing spread is considered as a threat to banana-based food and livelihood security in East Africa.
Inadequate knowledge of the underlying basis of observed morphological variation, genetic diversity, and population history of the EAHB germplasm genepool is an impediment to the improvement of EAHB, despite the socioeconomic importance of this staple crop. Here, we present a comprehensive analysis on the genetic diversity and population history of the EAHB subgroup of bananas. Using SSR markers, we have: (1) investigated associations between genetic diversity and morphological diversity of cultivars in the EAHB subgroup; (2) related EAHB genetic variability to that of other Musa subgroups and; (3) investigated support for recent population size changes among the EAHB since triploidization and subsequent domestication.
Materials and methods
EAHB samples and DNA extraction
Based on 73 morphological traits, the EAHBs have been classified into groups (referred to as clone sets) to reflect their variation in vegetative structures, bunch, fruit and male bud (Table 1). These are Nfuuka, Nakitembe, Musakala and Nakabululu (Karamura 1998). Sometimes cultivars of the cooking type become astringent and are no longer used for cooking but making beer. These are referred to as Mbidde (Table 1).
Ninety cultivars representing phenotypic diversity within and between the EAHB clone sets and including the beer types were obtained from field germplasm collections of EAHBs in Uganda and Kenya that are considered to represent the primary gene pool of EAHBs. These cultivars consisted of 48 cultivars from the Ugandan genebank and 42 cultivars from the Kenyan genebank of the triploid East African Highland banana (EAHB) as well as 4 AAB (African plantain) and 2 AAA (dessert) samples used as outgroups in this study (Supplementary Table 1). All 90 cultivars had unique phenotypic characteristics based on the IPGRI Musa descriptors used for germplasm management. All the EAHB specimens were classified in clone sets (Musakala, Nakitembe, Nfuuka, Nakabululu and Mbidde) according to the conventional criteria and fruit use (Karamura 1998). Genomic DNA was extracted from leaf samples of each specimen following modifications of two protocols; Dellaporta et al. (1983) and Mace et al. (2003). DNA was eluted in 100 µl low salt Tris–EDTA (TE; 1.0 mM EDTA, 10 mM Tris–HCL) buffer and diluted to 20 ng/µl working stocks based on spectrophotometric measurements.
DNA amplification of SSR and AFLP loci
Simple Sequence Repeats (SSRs) are a type of microsatellite markers that can be used for investigating population structure and history (Spencer et al. 2000; Amos and Hoffman 2010; Mariette et al. 2010; Galov et al. 2013; Hoban et al. 2013). Previously developed SSRs derived from banana ESTs (Crouch et al. 1997; Hippolyte et al. 2010; Mbanjo et al. 2012) (Supplementary Table 1) were used in this study. In total, 250 SSR markers were screened for polymorphism and a final set of 100 SSRs were selected for this study (Supplementary Table 2). The final 100 SSRs selected for this study were chosen based on the following criteria; (a) polymorphism; with more than one allele, (b) Multiallelic; and (c) clear scorable band with no stutters. PCR was used to amplify the SSRs. The 10 µl volume PCRs contained 1× standard Taq buffer with MgCl2; 0.2 mM dNTP mix; 0.5 units/µl Taq polymerase (New England Biolabs); 30 ng/µl genomic DNA template; and 0.3 µM fluorescently labeled primer. Amplification steps followed (1) initial denaturation at 95 °C for 3 min; (2) 40 cycles at 95 °C for 0.30, 1 min at 52–61 °C annealing temperature (primer pair specific), and 72 °C for 2 min; and (3) final extension of 20 min at 72 °C. All loci were individually amplified and the post-PCR primer products were multiplexed based on the dye and expected size of the fragment prior to capillary electrophoretic separation (ABI 3730xl DNA Analyzer), sizing (GeneScanTM-500 LIZ internal size standard) and manual verification of allele calling (Genemapper v4.1). The multiallelic information at each SSR locus was treated as binary data, so each SSR allele was treated as a separate marker (Christelova et al. 2011; de Jesus et al. 2013). We assessed the six outgroup cultivars representing other Musa acuminata subgroups separately from the set of 90 Kenyan and Ugandan EAHB.
A set of 13 AFLPs were also amplified in the same 90 Kenyan and Ugandan EAHBs as well as the six subspecies. AFLP analysis followed the procedure of Vos et al. (1995) modified to accommodate fluorescent visualization and using the restriction enzyme pair EcoRI/MseI (Supplementary Table 3). Total genomic DNA (1 µg) was simultaneously digested and ligated in a 40 µl reaction that included 5 units each of EcoRI, MseI, and T4 DNA ligase (New England Biolabs), 30 pmol of each EcoRI and MseI double-stranded DNA adaptor, 50 ng/μl BSA, and 50 mM NaCl in T4 Ligase buffer (New England Biolabs). Following complete digestion and ligation at room temperature, initial PCR enrichment of a subset of fragments (preamplification) used 5 μl of the digestion–ligation product as template: 0.5 μM of the EcoRI + 0/MseI + 0 primers (IDT, Coralville, IA, USA) and 0.25 U Taq DNA polymerase (New England Biolabs) in 20 μl deionized distilled water. PCR conditions were 2 min at 94 °C; 26 cycles of 94 °C for 1 min, 56 °C for 1 min; 72 °C for 1 min; followed by 5 min at 72 °C. The pre-amplification product was then diluted tenfold with deionized distilled water. Selective amplification reactions were similar to pre-amplifications, with 2 μl of diluted pre-amplification product used as template and substituting the 50 pM of the appropriate FAM/NED labeled EcoRI + 3 Mse1 + 3 selective AFLP primers. Thirteen selective primer combinations were used on all samples (Supplementary Table 4). PCR conditions were 2 min at 94 °C, 15 cycles of 30 s at 94 °C, 30 s at 65 °C dropping 1 °C per cycle, 1 min at 72 °C; then 23 cycles of 30 s at 94 °C, 1 min at 56 °C, 2 min at 72 °C; followed by 10 min at 72 °C. AFLP genotypes were electrophoresed and visualized with an ABI 3730 DNA analyzer. Bins within the range of 100–500 bp were manually generated for the amplified fragments (GeneMapper version 4.1; SoftGenetics LLCR, State College, PA, USA). We manually checked the quality of each AFLP fingerprint and bin using the method described by Whitlock et al. (2008) with slight modifications and restricted our analyses to fragments with relative florescence units greater than 100 to reduce background noise. We scored AFLP fragments manually for their presence (denoted as 1) and absence (denoted as 0).
Intra-EAHB population genetic variation
Levels of genetic diversity in the EAHB population were evaluated by calculating the average polymorphic information content (PIC) for each SSR locus as PIC i = 2f i (1 − f i ) where i is the information of the ith marker, f i is the frequency of the amplified allele (the presence of a band) and (1 − f i ) is the frequency of null alleles that have no band (Botstein et al. 1980; de Jesus et al. 2013). Variability was also assessed based on the average number of alleles per locus, percentage of alleles identical by state, the population allele frequency, and the expected heterozygosity with Powermarker v3.25. Confidence intervals were estimated by non-parametric bootstrapping.
To assess genetic differences between cultivars, we calculated the average number of alleles in each cultivar and private alleles per cultivar (de Jesus et al. 2013). Pairwise genetic distance between cultivars was calculated as the shared allele distance (D AS) with Powermarker v3.25: D AS values are linearly related to the time since common ancestry for a stepwise mutation model (SMM) (Goldstein et al. 1995). It was calculated as \( D_{\text{AS}} = \sum\nolimits_{j = 1}^{m} {\left( {\sum\nolimits_{t = 1}^{k} {{ \hbox{min} }\left( {p_{ij} ,q_{ij} } \right)} } \right)} \) where p ij and q ij are frequencies of ith allele at the jth locus, while k is the number of alleles at the jth locus, and m is the number of loci examined (Liu and Muse 2005).
The correlation of allele frequencies in the 90 EAHB was examined using a Bayesian framework with Structure v2.3.3 (Pritchard et al. 2000) that probabilistically assigned cultivars to genetically distinct clusters (K) and estimated admixture proportions for each cultivar in a population-free manner independent of a mutation model. The proportion of membership for each cluster permitted incomplete membership to minimize overfitting (Falush et al. 2007). To determine the most likely number of clusters, a range of values were tested (1 ≤ K ≤ 10). Analyses assumed an admixture model with correlated allele frequencies for a burn-in period of 105 steps prior to a run length of 105 with three independent iterations per K to confirm chain convergence (Pritchard et al. 2000). The second-order rate of change of the likelihood function (ΔK) was used to determine the most likely number of clusters (Evanno et al. 2005) with Structure Harvester (Earl 2011).
Population and clone set variability in the context of outgroups
To determine the genetic variability of cultivars within and among the two EAHB populations and five morphological clone sets, measures of genetic diversity were calculated: Shannon’s Information Index (Lewontin 1972), Nei’s genetic diversity (Nei 1973), the percentage of polymorphic bands, and the number of private alleles. Heterozygosity was defined as the mean number of polymorphic alleles. Genetic diversity was partitioned within (Hs) and among (Hb) groups for populations and clone sets. The population metrics were compared with the outgroup cultivars to provide a context for the level of population variation.
To investigate the proportion of the total variance among and within clone sets, we performed an analysis of molecular variance (AMOVA). Clone set pairwise PhiPT values were calculated to investigate the distribution of genetic differences among and within clone sets and to identify deviations from expected heterozygosity (Peakall and Smouse 2012). PhiPT was computed as Nei’s genetic diversity within clone sets divided by that within and among clone sets (equivalent to an F ST value). The number of migrants among clone sets in each generation (Nm) (Slatkin 1985) was calculated as Nm = (1 − PhiPT)/(2PhiPT).
The population structure of the EAHB set of 90 and the six outgroup cultivars was investigated using principal coordinates analysis (PCA) of pairwise SSR D AS values using R (http://www.r-project.org). This was repeated for the pairwise AFLP D AS values. The pairwise SSR D AS values were also visualized using phylogenetic networks of neighbor-net uncorrected p distances with SplitsTree v4 (Huson and Bryant 2006) to relate the population-level variation to that of the outgroups.
Inference of historical population sizes
To gain an insight into the evolutionary history of the EAHB, we compared a neutral hypothesis of a constant recent population size compared to an alternative one of a population expansion during domestication. Genetic signatures of a bottleneck associated with clonal propagation from a small founder population may be diminished during the subsequent recovery phase during which the population expands. Consequently, distinguishing a population in post-bottleneck recovery from one that was expanding after a small ancestral population size may not be possible for inbred groups with a single recent origin.
To gain an insight into the evolutionary history of the 90 samples, we investigated variation in the mutation rate across SSRs so that we could subsequently estimate the ancestral and recent effective population size (N e) using the estimated mutation rates with Beast v1.8 and Tracer v1.5 (Drummond 2005; Drummond and Rambaut 2007). We examined the parsimony-informative SSRs (27) that had at least three taxa per SSR as diploid data within the set of 90 EAHB with repeat lengths ranging from 1 to 115 for a one-phase site model (two-phase models may not be significantly superior, Sainudiin et al. 2004). A single representative from genetically identical taxa was used, meaning nine samples were omitted. The SSRs were modeled as unlinked microsatellites so that the mutation rate for each depended on the SSR length, mutation copy number change, and likely copy number mutation direction (Wu and Drummond 2011). Mutation rates were estimated using data for the full set of 96 (EAHB with outgroup cultivars) for these 27 SSRs assuming a constant population size for 9.5 × 107 MCMC iterations after a burn-in of 9.5 × 106 steps to ensure the effective sample size >100 for each SSR. This was repeated for the vegetatively propagated set of 90 EAHBs (assuming a constant population size for 8.6 × 107 MCMC iterations after a burn-in of 8.6 × 106 steps).
This comparison indicated the set of 96 provided a more accurate mutation rate calibration because of the higher total number of mutations, and so these mutation rate estimates were used as the SSR clock rates for an extended Bayesian skyline plot to infer N e for the 90 EAHB (5.8 × 107 MCMC iterations after a burn-in of 5.8 × 106) and the set of 96 with outgroups (6.1 × 107 iterations with a burn-in of 6.1 × 106) (Heled and Drummond 2008). Posterior density intervals were calculated to compute the relative changes in N e, estimated using the mean mutation rate across the complete set of 27 SSRs, over time scaled using generations.
An expanding population should have lower heterozygosity (Cornuet and Luikart 1996; Piry et al. 1999) caused by a higher incidence of rare alleles (Luikart et al. 1998) at selectively neutral loci like SSRs. In contrast, a population bottleneck would distort the allele frequency distribution such that low-frequency alleles (<0.1) would be lost more rapidly than ones at higher frequencies (Maruyama and Fuerst 1985; Luikart et al. 1998). Notably for triploid specimens, heterozygosity is robust to complex ploidy states or examining inbred samples (DeGiorgio et al. 2011). We examined the heterozygosity of each SSR relative to the observed number of alleles and sample size with Bottleneck v1.2.02 using coalescent simulations under two mutation models: stepwise (SMM) (Di Rienzo et al. 1994) and a two-phase model (TPM). For the TPM, the SSR alleles were geometrically distributed (Fu and Chakraborty 1998) with 90 % following an SMM and with a variance of 30 % for the 10 % of non-stepwise mutations (Amos and Hoffman 2010). Evidence for an expansion was examined using a standardized differences statistic (T 2) and standardized sign test (Cornuet and Luikart 1996).
Results
To determine the extent of genetic variation across the EAHB genepool, we amplified 100 SSRs across a population of 90 EAHBs (sourced from Kenyan and Ugandan germplasm collections) and across six African plantain and dessert bananas.
The East African Highland banana population is genetically uniform
Low levels of genetic variability were observed in the 90 EAHB cultivars compared to the outgroup cultivars. In total, 475 SSR alleles were scored in 100 SSR, 267 were discovered in the set of 90 EAHB cultivars, while 43.78 % of total were exclusively scored in the outgroup. Due to the triploid nature of the EAHBs, three alleles at each SSR locus were expected. In this study, the allelic pattern detected ranged from one to a maximum of six alleles. The 100 SSRs could be grouped into seven categories based on the number of alleles scored per locus (Table 2): 84 out of 100 SSRs had three alleles or less. Allelic homogeneity was observed in 86 % of the total alleles scored in EAHBs where cultivars showed identical allelic profiles, which we termed as the main allele pattern. In 24 out of the 90 cultivars, a few alleles showed allelic variations (deviations from the main allele pattern) where they occurred either as a loss of a main allele or new allele(s) (in addition or as substitution to the main allele) and cultivars either showed one or a combination of both variations. Emergence of new alleles in addition or as substitution to the main allele was more common (observed in 27 alleles) than the loss of main allele (observed in 15 alleles).
The set of 90 EAHBs had low genetic diversity as evidenced by the mean PIC (0.058) and gene diversity (0.070) values. This low level of genetic variability was further highlighted by a lack of genetic differentiation between pairs (D AS = 0.071, range 0.000–0.176); 81.3 % of the 90 cultivars had D AS < 0.1; while the mean minor allele frequency was just 0.05. Moreover, 58 % of alleles were identical by state, even though an average of 209.3 alleles per cultivar was sampled (from a minimum of 205 for the cultivar NyarLuo Ratong to a maximum of 214 for the cultivar Nakitembe Red).
Population-free clustering with Structure identified two genetically distinguishable groups (Evanno et al. 2005) which corresponded to the sub-populations obtained from the Kenyan and Ugandan germplasm collections (Fig. 1; Supplementary Table 4), with population membership probabilities greater than 0.8 (Pritchard et al. 2000).
Principal coordinates analysis (PCA) of the population of 90 and six outgroup cultivars (plantain and dessert) analyzed by SSRs showed little differentiation between the two EAHB populations (Kenya and Uganda, PC2 with 1.9 % of total variation) (Fig. 2). In contrast, the six outgroup cultivars were distributed across PC1 accounting for 94.2 % of diversity. This lack of resolution was also evident for the AFLP analysis (Supplementary Figure S1). Though much of the diversity was within subpopulations (Hs = 0.056; p < 0.0001) compared to between subpopulations (Hb = 0.052; p < 0.0001), the Uganda EAHB subpopulation was more diverse than the Kenyan one. The Ugandan EAHB subpopulation had a higher expected heterozygosity (0.065 vs 0.049) and proportion of polymorphic loci (13.5 vs 9.4 %), possibly suggesting that the Kenyan EAHB subpopulation may be derived from the Ugandan one. However, this may be complicated by genetic drift distinguishing the populations at few loci, and novel variants unique to certain cultivars (White Nakabululu and Mtore; Supplementary Figure S5).
No genetic differentiation of morphological groups of EAHBs
There was much higher genetic variation observed within the five morphological groups (clone sets) than between the five morphological clone sets (Hw = 0.0925 vs Hb = 0.022): 96 % of the variation was within clone set groups and only 4 % of the variation was between groups (Supplementary Table 6). This was supported by a lack of differentiation between clone sets (mean PhiPT = 0.036, p = 0.01 with a range of 0.011 and 0.125, Table 3). Furthermore, the maximum D AS value between clone set pairs was very low at 0.004 (Table 3).
Low genetic diversity within EAHB
Diversity within the two EAHB subpopulations was much lower compared to the outgroup cultivars. This was illustrated by PCA (Fig. 2) and was further highlighted in a phylogenetic network that partitioned the data in a similar manner: the set of 90 EAHBs form two closely related groups that are markedly homogeneous compared to the highly diverse six outgroups (Fig. 3; Supplementary Table 5). These results also demonstrated the differentiation of the EAHB from the plantains (AAB) and dessert (AAA) bananas—though MunjuP retained an intermediate classification suggesting progenitor(s) related to both cultivar sets.
Molecular evidence of a recent EAHB population expansion
In this study, we performed the first estimation of SSR mutation rates in bananas. Considerable variation in the estimated SSR mutation rates across 27 parsimony-informative SSRs was observed, with a mean of 0.00166 substitutions per SSR per generation, ranging from 0.00027 (locus 44) to 0.0030 (locus 47). This 11-fold magnitude of variation illustrated the heterogeneity associated with STR/SSR mutation rates (Scarcelli et al. 2013) (Supplementary Figure S3). In addition, somatic mutation rates estimated for the set of 90 EAHBs differed: these had a mean value of 0.00804 substitutions per SSR per generation, ranging from 0.00065 (locus 44) to 0.07620 (locus 70). Compared to the data for the entire set of 96, this produced an average excess of 4.8-fold, but this varied from 0.6 (locus 27) to 85.0-fold (locus 70), highlighting potential mutation rate differences between vegetatively propagated samples of different Musa acuminata parental origin.
We also investigated evidence for recent population size changes in the EAHBs. Using the mutation rates estimated at 27 SSRs for the set of 96, extended Bayesian skyline plots indicated a historical low constant N e with 95 % highest posterior density (HPD) values <4.9 individuals for both. Present generation N e estimates indicated a dramatic recent increase in N e for the sets of 90 EAHB (1435.7 with a 95 % HPD range of 1175–12,234) and 96 containing the outgroups (2868.6, 95 % HPD range 2352–35,301) (Supplementary Figure S4). Despite this recent jump in Ne, no statistically significant evidence of population size change was inferred using this dataset.
Our analysis detected a significant heterozygote deficiency for both SMM and TPM mutation rates, characteristic of a population expansion (Wilcoxon one-tailed p < 0.0005, Table 4) (Cornuet and Luikart 1996) or recovery after a decrease in size (McEachern et al. 2011). In addition, a significantly negative standardized differences value (T 2) was observed (SMM: T 2 = −3.774, p = 0.00008; TPM: T 2 = −2.668, p = 0.00381). Allele frequency distributions of the 43 polymorphic SSRs showed a shifted mode from the normal ‘L-shaped’ incompatible with a recent bottleneck (Supplementary Figure S2).
Discussion
The domestication of crop species from crop wild relatives has typically resulted in a genetic bottleneck and associated founder effect on genetic diversity in the primary cultivated genepool. For many crops, post-domestication breeding and artificial selection have allowed the introduction of new alleles and haplotype combinations through sexual recombination. However, the domestication of triploid crops can be associated with sterility, which generates a reproductive barrier to the introduction of novel germplasm (e.g., from wild relatives) and acts as a major constraint for sexual recombination based breeding programs. This is particularly the case for the triploid East African Highland bananas, which can be vegetatively propagated despite their seed sterility.
The majority of the current EAHB cultivars grown by almost 100 million farmers in East Africa have not arisen from formal breeding programs. Instead such varieties represent vegetatively propagated clones that have been transmitted over generations and exchanged between farmers for centuries. The phenotypic diversity for EAHBs that exists in the Great Lakes region of East Africa has led to the proposition that this region is a secondary center of diversity for EAHBs. To capture the diversity of the EAHB primary genepool, both the field genebank EAHB germplasm collections in Uganda and Kenya were established in the 1990s from germplasm collected from farmers fields across East Africa. It is possible that the morphological variation between the ~120 morphologically distinct cultivars of EAHB in these germplasm collections has arisen due to somaclonal mutations which were selected and propagated as somaclonal mutants by farmers.
Despite their importance for food and livelihood security, little is known about the genetic diversity and history of the EAHB (AAA) subgroup of bananas. In this study, we used 100 microsatellite markers (and AFLPs) to investigate the genetic diversity, population structure and evolutionary history of 90 phenotypically diverse EAHB cultivars (representative of the EAHB primary genepool) obtained from Ugandan and Kenyan germplasm collections. Our study included six cultivars (four plantains and two dessert samples) representing different Musa acuminata subgroups.
The cultivated EAHB primary genepool is genetically uniform
Although substantially reduced genetic diversity has been reported within other Musa groups (Creste et al. 2003; Hippolyte et al. 2012), our study indicates that EAHBs exhibit significantly lower genetic variability than other Musa groups (El-Khishin et al. 2009; Opara et al. 2010; Christelova et al. 2011; Resmi et al. 2011; Abdullah et al. 2012; Shaibu 2012; de Jesus et al. 2013). In addition, while EAHBs are classified into four clone sets based on morphological characteristics, our study indicated that the morphological classification system was not reflected at the level of genetic diversity and relatedness. Our population classification and phylogenetic analysis showed minimal differentiation of the cultivars in the Ugandan and Kenyan collections, suggesting that the cultivars in both these germplasm collections have a recent single origin, more likely to be ancestral to the Ugandan set on the basis of its comparatively higher genetic variability.
No association between morphological classification systems and genetic diversity of EAHB cultivars
In this study, we have investigated whether there is an association between genetic diversity and morphological diversity for EAHBs and find no evidence for such an association. This raises the question of the underlying basis for the morphological variation observed between the EAHB cultivars and clone sets (Table 1). It is possible that the morphological differences observed between EAHBs represent rare somatic mutations (e.g., point mutation, transposon insertions/excisions) that could not be directly detected by low-density markers such as SSRs or AFLPs) that affect the morphological trait used for classification (e.g., fruit size, fruit length, stem color, etc.). Where they exist, the identification of genomic loci and mutations underlying the morphological traits used as descriptors for EAHBs would require experimental designs involving mapping populations, bulked segregant and/or genome wide association approaches. In addition, studies on heritability of the morphological traits that differ across EAHBs would be necessary to inform such approaches. The advent of higher density genotyping by sequencing (GBS) approaches may facilitate the identification of loci underlying heritable morphological differences between EAHBs. Alternatively (or in addition), the morphological variation in EAHBs could be arising due to mitotically heritable epigenetic variation (arising from environmental and/or human selection effects) across vegetative propagation cycles.
Irrespective of the underlying mechanistic basis of the morphological traits used to classify EAHBs, our study demonstrates that the cultivated EAHB genepool is genetically uniform. As a result, EAHB cultivation, consumption and associated food security in East Africa are likely to be vulnerable to neo-virulent pests and pathogens of EAHBs.
The broadening of the genetic base of EAHB production (while maintaining and improving quality and yield characteristics of EAHBs) remains an important priority. Despite the inherent difficulties for polyploid breeding in EAHBs, EAHB breeding efforts in East Africa are engaged in efforts to introduce new genetic variation into EAHBs (Ortiz and Swennen 2014). Musa variation is ultimately derived from four wild species: M. acuminata (A genome), M. balbisiana (B), M. schizocarpa (S) and M. textilis (T)—of which the latter two show extensive genetic but not phenotypic variation (de Jesus et al. 2013), and among whom only M. acuminata is parthenocarpic (Heslop-Harrison and Schwarzacher 2007; Kennedy 2008).
EAHBs likely arose from a single ancestral clone followed by a recent population expansion by vegetative (asexual) propagation by farmers
Banana domestication may have occurred up to 10,000 years ago (Perrier et al. 2011). However, movement from primary centers of diversity, such as from Papua New Guinea to Africa, may have occurred much recent (e.g., about 2000–2500 years ago) (Perrier et al. 2011). It is currently not known whether the triploid origin of EAHBs occurred in Papua New Guinea or in Africa post-introduction. In either scenario, the generation of the initial triploid ancestors was most likely followed by ongoing human selection of new clones (as somatic mutants that were vegetatively propagated).
Our simulations of the recent historical effective population size and expected heterozygosity are symptomatic of a recent EAHB population expansion (Cornuet and Luikart 1996). Although low allelic diversity may be caused by a genetic bottleneck (Gebremedhin et al. 2009), the EAHB sample set did not support this directly, consistent with previous work (Li et al. 2013)—though the ancestral population size was extremely small. This genetic pattern may be accentuated by a Meselson effect of heterozygous alleles persisting in an asexual reproductive system (Butlin 2002). A recent expansion of EAHBs could be due to human-mediated establishment of EAHBs in new agro-environments across East Africa (Schoebel et al. 2014).
The intermediate phylogenetic placement of the cultivar MunjuP between the plantains, dessert and EAHBs could suggest ancient interbreeding events (Bryant and Moulton 2004), which is supported by previous work on African plantains and EAHBs, which may share at least one maternal A genome derived from M. acuminata subspecies banksii (Kennedy 2008). The authors recognize that while common descent does not, sensu strictu, imply interbreeding events, it does suggest that these genomes had a common genome donor parent. The observation of historical mixing events across subspecies suggests that deeper molecular investigation of these subspecies can help to determine the hybrid origins of extant cultivars, for which the number and timing ancient migrations both before (in diploids) and after hybridization (in triploids) remain unknown (de Jesus et al. 2013).
The differences between the 90 EAHB cultivars across geographical regions (Kenya and Uganda) were minimal relative to the higher diversity in the plantain and dessert cultivars. Introgression of genetically distinct cultivars at low levels would have erased the genetic homogeneity in the EAHBs (Miller et al. 2012). Yet, no evidence for multiple origins or admixture was observed in our study. Our results support our hypothesis of a single seed origin for EAHBs, consistent with a hybridization event, followed by selection and subsequent clonal propagation by farmers over centuries and across environments.
Our study strongly supports the hypothesis that the EAHB arose from genetically monomorphic clones, likely selected during domestication. We consider it likely that wild and cultivated diploids should harbor an array of genetically diverse clones. For further studies, a finer delineation of the events associated with the triploidization and domestication of EAHB will be achieved by comparing genetic variation in EAHB (AAA), plantain (AAB) and dessert (AAA) subspecies, to the genetic variation in M. acuminata wild and edible diploid (AA) and triploid (AAA) samples. Overall, our SSR and AFLP study conclusively demonstrates that the cultivated EAHB is genetically uniform, most likely arising from a single recent triploid origin followed by somaclonal vegetative reproduction by farmers across East Africa.
Author contribution statement
CS and JL designed and supervised the research. MK collected samples and conducted lab work. Sample collection assistance was provided by DK, MN and MO. Data analysis and manuscript drafting was conducted by MK, TD, JL, MF and CS. Sample collection assistance was provided by DK and MO. Ph.D. research supervision of MK by JL, MF and CS.
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Acknowledgments
This research was conducted at Biosciences Eastern and Central Africa (BecA)-ILRI and in the Genetics and Biotechnology Lab at the Plant and AgriBiosciences Research Centre (PABC), School of Natural Sciences, National University of Ireland Galway. We thank Dr Kassa Semagn for his invaluable help in data analysis. This research was supported by funding from Irish Aid and the International Institute for Tropical Agriculture (IITA).
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Communicated by A. H. Schulman.
M. Onyango: Deceased (RIP).
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Kitavi, M., Downing, T., Lorenzen, J. et al. The triploid East African Highland Banana (EAHB) genepool is genetically uniform arising from a single ancestral clone that underwent population expansion by vegetative propagation. Theor Appl Genet 129, 547–561 (2016). https://doi.org/10.1007/s00122-015-2647-1
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DOI: https://doi.org/10.1007/s00122-015-2647-1