Abstract
Polychlorinated biphenyls (PCBs) are persistent organic pollutants associated with metabolic disruption and nonalcoholic fatty liver disease (NAFLD). Based on their ability to activate the aryl hydrocarbon receptor (AhR), PCBs are subdivided into two classes: dioxin-like (DL) and non-dioxin-like (NDL) PCBs. Previously, we demonstrated that NDL PCBs compromised the liver to promote more severe diet-induced NAFLD. Here, the hepatic effects and potential mechanisms (by untargeted liver proteomics) of DL PCBs and NDL PCBs or co-exposure to both in diet-induced NAFLD are investigated. Male C57Bl/6 mice were fed a 42% fat diet and exposed to vehicle control; Aroclor1260 (20 mg/kg, NDL PCB mixture); PCB126 (20 μg/kg, DL PCB congener); or a mixture of Aroclor1260 (20 mg/kg) + PCB126 (20 μg/kg) for 12 weeks. Each exposure was associated with a distinct hepatic proteome. Phenotypic and proteomic analyses revealed increased hepatic inflammation and phosphoprotein signaling disruption by Aroclor1260. PCB126 decreased hepatic inflammation and fibrosis at the molecular level; while altering cytoskeletal remodeling, metal homeostasis, and intermediary/xenobiotic metabolism. PCB126 attenuated Aroclor1260-induced hepatic inflammation but increased hepatic free fatty acids in the co-exposure group. Aroclor1260 + PCB126 exposure was strongly associated with multiple epigenetic processes, and these could potentially explain the observed nonadditive effects of the exposures on the hepatic proteome. Taken together, the results demonstrated that PCB exposures differentially regulated the hepatic proteome and the histologic severity of diet-induced NAFLD. Future research is warranted to determine the AhR-dependence of the observed effects including metal homeostasis and the epigenetic regulation of gene expression.
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Introduction
The pollutants, polychlorinated biphenyls (PCBs), are metabolism-disrupting chemicals associated with nonalcoholic fatty liver disease (NAFLD), obesity, dyslipidemia, diabetes, and cardiovascular disease (Raffetti et al. 2018; Rosenbaum et al. 2017; Everett et al. 2011; Cave et al. 2010; Goncharov et al. 2008; Heindel et al. 2017). PCBs were produced during the 1930s–1970s for use in multiple industrial applications (Addison 1983). Although intentional PCB production is banned, their high thermodynamic stability imparting resistance to metabolism and degradation make PCBs persistent organic pollutants. PCBs bioaccumulate in living organisms and exposures increase along trophic levels of the food chain. PCBs have been detected in the serum of 100% American adult participants in the National Health and Nutrition Examination Survey (NHANES) (Cave et al. 2010).
The 209 possible PCB congeners have been generally categorized into two major classes: “coplanar” and “noncoplanar”. Coplanar PCBs (no or one chlorine atom in the ortho-position) including PCB126 potently activate the aryl hydrocarbon receptor (AhR), thereby eliciting a dioxin-like (DL) response similar to TCDD, and are known as “DL” PCBs (Safe et al. 1985). In contrast, noncoplanar PCBs have two or more chlorine atoms at the ortho-position, conferring a conformation that precludes AhR binding and activation. However, these so-called “non-DL” (NDL) PCBs activate other xenobiotic receptors, such as the constitutive androstane receptor (CAR) and elicit a phenobarbital-like response (Safe et al. 1985). Commercially, PCBs were sold as mixtures rather than individual congeners. Arclor1260 (60% chlorine by weight) was a first-generation PCB mixture manufactured in Anniston Alabama, by Monsanto. The Anniston Community Health Survey I reported a high prevalence of suspected NAFLD associated with PCB congener exposures in Anniston residents (Clair et al. 2018). The PCB congeners that constitute Aroclor1260 are ones that have higher molecular weights and are not easily metabolized; therefore, they tend to bioaccumulate in living organisms including humans. In fact, the PCB composition in Aroclor1260 more closely resembles human bioaccumulation patterns than any other single commercially produced Aroclor mixture (Wahlang et al. 2014a). Because low-dose Aroclor1260 does not activate either the human or murine AhR, Aroclor1260 has previously been used to model NDL PCB exposures (Wahlang et al. 2014a, b, 2016).
PCB exposures are associated with NAFLD in human cohort studies and either cause or exacerbate diet-induced NAFLD in animal studies (reviewed in (Wahlang et al. 2019a)). NAFLD is a broad spectrum of progressive liver disorders ranging from fat accumulation (steatosis) to hepatic inflammation (steatohepatitis), fibrosis, cirrhosis, and hepatocellular carcinoma. NAFLD and its more severe form, nonalcoholic steatohepatitis (NASH), can be due to various etiological factors, such as high-caloric intake, lifestyle habits, and more recently, environmental chemical exposures. Our group coined the term “toxicant-associated steatohepatitis” (TASH) to reflect the steatohepatitis associated with industrial chemical exposures (Wahlang et al. 2019b). However, recent studies have implicated known PCB receptors such as the AhR, CAR, pregnane-xenobiotic receptor (PXR), and epidermal growth factor receptor (EGFR) in PCB-induced TASH (Wahlang et al. 2019a).
In the liver, AhR activation by TCDD and PCB126 increased lipid accumulation and led to simple steatosis in short-term, rodent studies (Angrish et al. 2012; Hardesty et al. 2019b). CAR, along with PXR, function as sensors of xenobiotic substances and are primarily involved in detoxification and foreign compound metabolism. However, previous studies from our group and others have shown that NDL PCB-mediated CAR and PXR activation also play a fundamental role in regulating hepatic energy metabolism and may contribute to TASH development (Wahlang et al. 2019a). Like phenobarbital, some PCBs appear to indirectly activate CAR by diminishing EGFR phosphorylation, leading to reduced hepatic phosphoprotein signaling and metabolic disruption in TASH (Hardesty et al. 2017, 2019a, b). Nonetheless, these studies only evaluated the effects of either DL or NDL PCBs in diet-induced obesity, although both classes of PCBs are concurrently present in the human exposome. We recently developed a new PCB exposure model, by spiking the potent AhR agonist, PCB126, into Aroclor1260 (Hardesty et al. 2019b). This mixture produced different hepatic effects than either Aroclor1260 or PCB126 alone in an acute exposure model (Hardesty et al. 2019b). Therefore, the objective of the current study is to evaluate the modulation of diet-induced NAFLD by several different types of chronic PCB exposures. Similar to our previous model (Hardesty et al. 2019b), these exposures include low-dose Aroclor1260 (representative NDL PCB mixture), PCB126 (prototypical DL PCB), and Aroclor1260 + PCB126 (NDL + DL PCB mixture). Untargeted liver proteomics was performed to elucidate potential mechanisms for observed differences in NAFLD disease severity.
Materials and methods
Animal studies
The related animal protocol was ratified by the University of Louisville Institutional Animal Care and Use Committee. Adult male C57BL/6 mice (8 weeks old) were purchased from Jackson Laboratory and distributed into four equal groups (n = 10). All mice were fed a high-fat diet (HFD, 15.2, 42.7, and 42.0% of total calories from protein, carbohydrate, and fat, respectively, TekLad TD88137) throughout the study period. At 10 weeks of age, ten mice in each group were given either corn oil, Aroclor1260 (20 mg/kg), PCB126 (20 μg/kg), or a mixture of Aroclor1260 (20 mg/kg) plus 0.1% PCB126 (20 μg/kg) via a one-time gavage and followed for 12 weeks (Supplementary Fig. 1). At week 8 post gavage, a glucose tolerance test (GTT) was performed as described previously (Wahlang et al. 2019c). Dual energy X-ray absorptiometry (DEXA) scanning (Lunar PIXImus densitometer, WI) was performed to measure body fat composition before euthanasia. Whole blood for plasma, liver, and fat tissue samples was harvested after euthanasia.
Definition of PCB doses utilized
The doses of the different PCBs used in the present study were similar to those used in the acute study (Shi et al. 2019) and were designed to mimic doses relevant to human exposures. For example, Aroclor1260 at 20 mg/kg was designed to reflect serum PCB levels measured in the highest exposed quartile of the ACHS cohort (Silverstone et al. 2012), while PCB126 at 20 μg/kg (0.1% of Aroclor1260) was designed to mimic the percent of serum PCB126 measured in NHANES 2003–2004, relative to other heavily bioaccumulated PCBs such as PCB153, the congener with the highest reported serum levels in NHANES 2003–2004 (Serdar et al. 2014).
Histological staining
Liver and pancreas tissues were fixed in 10% neutral buffered formalin for 72 h and embedded in paraffin for routine histological examination. Hematoxylin–eosin (H&E) staining was performed to identify histopathological changes. Chloroacetate esterase (CAE) activity, macrophage accumulation, and fibrosis were evaluated by CAE, immunohistochemical staining, and picro sirius red staining, respectively, according to the manufacturer’s protocols. Micrographic images were obtained by a high-resolution Olympus digital scanner with an Olympus digital camera (BX41).
Real-time PCR
Mouse liver and pancreas tissues were homogenized and total RNA was extracted using RNA-STAT 60 reagent according to the manufacturer’s protocol. The purity and quantity of total RNA were measured with a Nanodrop spectrometer (ND-1000, Thermo Fisher Scientific, Wilmington, DE) using ND-1000 V3.8.1 software and cDNA was reverse transcribed from 1 μg RNA using a one-step cDNA synthesis reagent (QScript cDNA Supermix, QuantaBio, Beverly, MA). RT-PCR was performed on the CFX384TM Real-Time System (Bio-Rad, Hercules, CA) using iTaq Universal Probe Supermix and Taqman probes as described elsewhere (Wahlang et al. 2019c). The relative mRNA expression was calculated using the comparative 2−▵▵Ct method and normalized against GAPDH mRNA.
Measurement of hepatic lipids, plasma lipids, and cytokines
Hepatic lipids were extracted by a mixture of chloroform and methanol (2:1) according to a published protocol (Bligh and Dyer 1959). Triglycerides and free fatty acid contents were measured using commercial kits with final values normalized to liver weight. Plasma alanine transaminase (ALT), aspartate transaminase (AST), triglyceride, cholesterol, and lipoproteins were quantified with lipid panel plus kits on a Piccolo Xpress Chemistry Analyzer (Abbott Laboratories, IL). Plasma cytokine and adipokine levels were acquired using a customized Milliplex® MAP mouse adipokine panel on a Luminex® 100 system (Luminex Corp, Austin, TX).
Proteomics analysis
Proteins were extracted from liver tissue in RIPA buffer supplemented with protease and phosphatase inhibitors using a bead homogenizer and protein amounts were quantitated by BCA assay. Protein lysates (200 μg) were trypsinized using the modified filter-aided sample preparation method (Wisniewski et al. 2009) and enriched for phosphopeptides by the TiO2–SIMAC–HILIC method (Engholm-Keller and Larsen 2016). Briefly, protein samples were reduced with dithiothreitol, denatured with 8 M urea, and alkylated with iodoacetamide followed by centrifugation through a high molecular weight cutoff centrifugal filter (Millipore, 10k MWCO). After overnight digestion with sequencing grade trypsin (Promega), the digested proteins were collected and cleaned with a C18 Proto™ 300 Å Ultra MicroSpin column. Protein digested samples (50 μg) were labeled with tandem mass tag (TMT) TMT10plex™ Isobaric Label Reagent Set (Thermo Fisher, Waltham, MA); samples were then concentrated and desalted with Oasis HLB Extraction cartridges (Waters Corporation, Milford, MA) using a modified protocol for extraction of the digested peptides. (Keshishian et al. 2015). Samples were then subjected to high pH reversed phase separation with fraction concatenation on a Beckman System Gold LC system supplemented with 126 solvent module and 166 detector in tandem with a Bio-Rad Model 2110 Fraction Collector (McDowell et al. 2013). Liquid chromatography/mass spectrometry was used to measure TMT-labeled peptides. Briefly, every high pH reversed phase fraction was dissolved in 50 µL solution of the combination of 2% v/v acetonitrile/0.1% v/v formic acid and 1 µL of each fraction was analyzed on EASY-nLC 1000 UHPLC system (Thermo Fisher) and an Orbitrap Elite—ETD mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). Proteome Discoverer v2.2.0.388 was used to analyze the raw data collected from the mass spectrometer. Hepatic proteins that had significance abundance were imported into MetaCore software (Clarivate Analytics, Philadelphia, PA) for the following analyses: gene ontology (GO), enrichment by protein function (EPF), and interaction by protein function (IPF).
Statistical analysis and data sharing
Statistical significance was determined by two-way analysis of variance using GraphPad Prism version 7.02 for Windows (GraphPad Software Inc., La Jolla, CA, USA). p < 0.05 was considered statistically significant. Statistical analysis for the proteome data was analyzed using the R package as described previously (Srivastava 2019a, b, c). Given the exploratory nature of the study, significantly altered proteins were further filtered using an FDR threshold of 0.2 and proteins exhibiting a fold change of −0.5 < log2FC < 0.5 were rejected to rule out false positives. Proteomics data files were deposited with MassIVE (http://massive.ucsd.edu/) data repository, Center for Computational Mass Spectrometry at the University of California, San Diego, and shared with the ProteomeXchange (www.proteomexchange.org).
Results
Effects of PCB exposures on body composition, glucose tolerance, and adipokines
Body weight was measured weekly throughout the 12-week study. There was a gradual increase in body weight in all the four groups from week 0 to week 12 (Fig. 1a). However, there were no significant differences in the amount of body weight gain between the groups (Fig. 1b). Body fat composition was measured using both DEXA scan and by weighing the harvested epididymal fat (white adipose tissue) content. Although there was no significant difference in overall percent fat composition (Supplementary Fig. 2), Aroclor1260 exposure, however, increased white adipose tissue to body weight ratio (Fig. 1c). In addition, neither Aroclor1260 nor PCB126 affected liver weight to body weight ratio (Fig. 1d) and pancreas weight to body weight ratio in these mice (Supplementary Fig. 2). A GTT was performed to examine PCB effects on glucose metabolism; there were no differences between groups for alteration in glucose uptake (Supplementary Fig. 2). PCB effects on plasma lipids and adipocytokines were evaluated. The Aroclor1260 + PCB126 group significantly decreased plasma cholesterol levels via its interacting Aroclor1260 and PCB126 components. PCB126 exposure decreased triglyceride levels in the Aroclor1260 + PCB126 group (Supplementary Table 1). There was no PCB effect on adipokines including adiponectin, leptin, and resistin (Supplementary Table 1)
Effects of PCB exposure on hepatic steatosis, inflammation, injury, and fibrosis
Hepatic steatosis was assessed using H&E staining of liver sections. All HFD-fed mice developed variable, centrilobular, macrovesicular steatosis and the different PCB exposures did not exacerbate HFD-induced steatosis (Fig. 2a). Hepatic lipids were measured and PCB exposures had no effect on either hepatic triglyceride or cholesterol levels (Supplementary Fig. 3). However, hepatic free fatty acid levels were significantly increased by the Aroclor1260 + PCB126 mixture (Fig. 2b). Histological analyses for liver inflammation were assessed using CAE and F4/80 staining. CAE staining demonstrated that HFD feeding induced neutrophil infiltration in the liver (Fig. 2c); however, Aroclor1260 exposure exacerbated this effect as shown by the number of inflammatory foci counted per field (Fig. 2d). Interestingly, the Aroclor1260 + PCB126 group showed a lesser number of inflammatory foci counted per field. Likewise, F4/80 staining demonstrated that the Aroclor1260 group had increased macrophage infiltration compared with any other group (Fig. 2e). This observation was consistent with hepatic Cd68 gene expression where in Aroclor1260 increased Cd68 mRNA levels, indicating a hepatic inflammatory state, while this effect was absent with PCB126 exposure (Fig. 2f). Commonly used biomarkers of liver injury, namely plasma ALT and AST levels, were measured. Elevated plasma ALT activity levels were only seen with Aroclor1260 exposure, but not with PCB126 (Fig. 2g) while PCB126 showed a trend for decreased plasma AST activity levels (Supplementary Fig. 3). In addition, the Aroclor1260 + PCB126 group showed attenuation of Aroclor1260-elevated plasma ALT levels, suggesting that PCB126 may be protective against liver injury in this model. Fibrosis was assessed by picro sirius red staining of liver sections to detect collagen deposition (Supplementary Fig. 4). While there were no histological differences between groups, PCB126 decreased the hepatic gene expression of collagen (Col1a2) and actin 2 (Acta2), implicating a suppression of profibrotic gene expression in the liver (Supplementary Fig. 4)
PCB exposures modified expression of AhR and CAR target genes
Known PCB receptor activation such as AhR and CAR activation was examined. PCB126 exposure caused upregulation of cytochrome P450 1a2 (Cyp1a2), an AhR target gene, indicative of AhR activation. In contrast, the Aroclor1260 + PCB126 group showed decreased Cyp1a2 mRNA levels (Fig. 3a). With regards to AhR gene expression, PCB126 alone showed a trend for decreased AhR mRNA levels (Fig. 3b). For CAR activation, the CAR target gene, Cyp2b10, was measured. The Aroclor1260 group showed upregulated Cyp2b10 mRNA levels, indicative of CAR activation (Fig. 3c), while CAR expression was increased in the Aroclor1260 + PCB126 groups (Fig. 3d).
Effects of PCB exposure on the hepatic proteome
The various PCB exposures produced distinct hepatic proteomes (Fig. 4). A total of 8355 unique proteins and their isoforms were detected, corresponding to 4609 protein groups. Liver protein content was regulated as follows: Aroclor1260 (35 increased and 48 decreased); PCB126 (158 increased and 238 decreased); and Aroclor1260 + PCB126 (144 increased and 257 decreased) (Fig. 4 and Supplementary Table 2). The majority of differentially abundant proteins for each group were unique to that exposure (range 58.7–63.1%). Of the 401 proteins associated with Aroclor1260 + PCB126 exposure, only 5.9% were also associated with Aroclor1260, while only 33.6% were also associated with PCB126. Only three proteins were changed in all three PCB exposure groups (BET1-like protein and two isoforms of protein-O-linked-mannose-β-1,4-N-acetylglucosaminyltransferase 2). Clearly, the proteomic changes associated with the Aroclor1260 + PCB126 mixture were not the sum of its parts.
EPF analysis was performed to determine the protein classes most impacted by PCBs (Supplementary Table 3). Enzymes were the most affected class (Aroclor1260, z-score = 3.63; PCB126, z-score = 7.11; Aroclor1260 + PCB126, z-score = 4.89). Consistent with this observation and the RT-PCR data, the enzyme levels of CYP1A1 and CYP1A2 were higher for PCB126 (CYP1A1: 9.09-fold, p = 7.10E−06 and CYP1A2: 5.85-fold, p = 3.10E−08) and, to a lesser degree, for Aroclor1260 + PCB126 (CYP1A2: 2.39-fold, p = 2.39E−08) (Supplementary Table 2). While Aroclor1260 increased hepatocyte injury (by histologic and plasma ALT enzyme activity biomarkers), plasma AST activity was not significantly increased. This could be related to the peculiar proteomic observation that hepatic AST enzyme levels were reduced by PCB exposures (Aroclor1260: 0.64-fold, p = 2.18E−03 and PCB126 0.68-fold, p = 5.82E−03). Again, consistent with the liver histology, inflammation-associated enzymes and leukocyte markers were increased with Aroclor1260 (e.g., macrosialin/CD68, 2.26-fold, p = 1.85E−04) and decreased with either PCB126 (e.g., myeloperoxidase: 0.51-fold, p = 1.79E−03; α-defensin 20: 0.48-fold, p = 2.90E−02; and the calprotectin component, protein S100-A8: 0.32-fold, p = 8.05E−03) or Aroclor1260 + PCB126 (e.g., protein S100-A9: 0.62-fold, p = 4.35E−02; neutrophil granule protein: 0.58-fold, p = 1.17E−02; protein jagunal homolog 1: 0.33-fold, p = 1.20E−02; and neutrophil gelatinase-associated lipocalin (0.30-fold, p = 2.41E−02) (Supplementary Table 2). EPF revealed that Aroclor1260 altered protein levels of slightly more transcription factors than expected (z-score = 1.67), while the other exposures changed fewer than expected. Several nuclear receptors implicated in steatohepatitis were differentially regulated including peroxisome proliferator-activated receptor alpha (PPARα, Aroclor1260: 0.7-fold, p = 1.97E−03), liver X receptor alpha (PCB126: 1.47-fold, p = 1.81E−02), and V-erbA-related protein 2 (PCB126: 0.69-fold, p = 1.14E−02 and Aroclor1260 + PCB126: 0.63-fold, p = 2.69E−03). Thus, while each PCB exposure was associated with a distinct proteome, EPF demonstrated that these proteomes were consistent with the observed phenotypes.
Enrichment by GO processes (Fig. 5) revealed that the distinct proteomes associated with PCB126 and Aroclor1260 + PCB126 exposures were associated with similar top GO processes, although some differences were apparent. However, the top GO processes associated with Aroclor1260 were unique to that exposure. These findings are again consistent with the histology and phenotyping data. A very limited number of GO processes were enriched by Aroclor1260 including “negative regulation of receptor internalization”; “negative regulation of catabolic process”; aspartate catabolic metabolism; and polyadenylation-dependent RNA catabolic processes (Fig. 5). In contrast, hundreds of GO processes were significantly enriched by PCB126 and/or Aroclor1260 + PCB126 (data not shown). Both exposures enriched: (i) cellular, metabolic, and catabolic processes; (ii) protein metabolism processes; (iii) cellular localization and organization processes; and (iv) the “negative regulation of neurotransmitter (acetylcholine) secretion”. These processes, however, were enriched to a greater degree by Aroclor1260 + PCB126. In contrast, while both exposures enriched xenobiotic metabolism processes and lipid metabolism processes, these were enriched to a greater degree by PCB126.
GO processes related to cytoskeletal remodeling/fibrosis and metal homeostasis were enriched by PCB126 only (Fig. 5). The abundance of several cytoskeletal-associated proteins were markedly increased by PCB126 including troponin C (120.33-fold, p = 1.30E−03); actin α, skeletal muscle (55.47-fold, p = 7.38E−03); myosin light chain 3 (33.67-fold, p = 1.27E−02); LIM domain-binding protein (15.24-fold, p = 7.88E−03), etc. (Supplementary Table 2). However, other cytoskeletal proteins, including type II keratins, were differentially regulated by Aroclor1260 (keratin 5: 0.28-fold, p = 2.12E−03) or Aroclor1260 + PCB126 (keratin 2: 0.30-fold, p = 3.85E−02; keratin 4: 0.62-fold, p = 4.19E−02; and keratin 6a: 1.89-fold, p = 1.34E−02). While the PCB exposures were not associated with fibrosis at the histologic level, PCB126 exposures were associated with reduced expression of several profibrogenic genes at the molecular level. Consistent with this observation, PCB126 was associated with reduced abundance of several proteins associated with liver fibrosis including collagen α-1(IV) chain (0.68-fold, p = 2.26E−02); prolyl 4-hydroxylase subunit alpha-1 (0.66-fold, p = 1.42E−03); and procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (0.47-fold, p = 2.28E−02). Multiple proteins associated with metal homeostasis were likewise reduced only by PCB126 including ferritin light chain 1 (0.64-fold, p = 1.44E−02), light chain 2 (0.63-fold, p = 1.24E−02), and heavy chain (0.60-fold, p = 6.11E−03), and zinc transporter 4 (0.55-fold, p = 3.09E−02).
Several GO processes related to the epigenetic mechanisms were enriched only by Aroclor1260 + PCB126 (Fig. 5). These included “regulation of gene expression, epigenetic”, “negative regulation of chromatin silencing”, and “histone H3-K27 methylation”. Differentially abundant proteins contributing to these observations included reduced histone deacetylase 2 (0.68-fold, p = 1.13E−02); reduced histones H1.2 (0.63-fold, p = 5.98E−04), H1.4 (0.66-fold, p = 8.87E−03), H1.5 (0.67-fold, p = 2.45E−02), and H1t (0.67-fold, p = 1.40E−02); reduced RNA polymerase-associated protein CTR9 homolog (0.60-fold, p = 7.64E−04); and reduced exportin 5 (0.66-fold, p = 2.07E−02). However, several other epigenetics proteins were reduced by PCB126 and Aroclor1260 + PCB126, such as RNA binding protein 3 (PCB126: 0.68-fold, p = 2.64E−03; Aroclor1260 + PCB126: 0.69-fold, p = 2.78E−03); LIM domain-containing protein 1 (PCB126: 0.66-fold, p = 6.66E−03; Aroclor1260 + PCB126: 0.63-fold, p = 2.93E−03); and protein arginine methyltransferase 7 (PCB126: 0.64-fold, p = 9.69E−04; Aroclor1260 + PCB126: 0.70-fold, p = 4.74E−03).
IPF analysis was performed and the top overconnected interactions by z-score are provided in Fig. 6. Seven unique objects, all protein kinases, were overconnected with Aroclor1260 exposure. These kinases included the mammalian target of rapamycin (mTOR); the adenylate kinase isoenzyme, mitochondrial (AK3L1); the alternate mitogen-activated protein kinase, p38γ; and protein kinase C α (PKCα). PCB126 and/or Aroclor1260 + PCB126 exposures were associated with 18 overconnected objects (Fig. 6). Eleven of these objects were transcription factors and eight of these were common between the two groups. Shared transcription factors included protein C-ets-1 (ETS1), SRY-box transcription factor 17 (SOX17), and GA binding protein transcription factor subunit α (GABPα); glucocorticoid receptor; zinc finger protein x-linked (ZFX); neuroblastoma MYC oncogene (N-Myc); and acute myeloid leukemia 1 (AML1). The z-scores were all higher with the Aroclor1260 + PCB126 exposure. Unique transcription factors overconnected by PCB126 included zinc finger protein, FOG family member 1 (ZFPM1), and CAR, while cellular oncogene FOS (c-FOS) was uniquely overconnected by Aroclor1260 + PCB126. PCB126 exposure was uniquely overconnected with the bradykinin precursor, kininogen 1, and thrombin. Aroclor1260 + PCB126 was uniquely overconnected with two enzymes associated with steatohepatitis and liver cancer, protein arginine methyltransferase 1 (PRMT1), and hypoxia-associated factor (SART1) (Zhao et al. 2019; Koh et al. 2016).
PCB effects on hepatic intermediary metabolism and plasma profile
Proteomics analysis revealed that PCBs played a role in dictating intermediary metabolism such as lipid and protein processes. To further validate these findings, hepatic expression of genes involved in a variety of metabolic processes, including lipogenesis, lipid transport and mobilization, fatty acid β-oxidation, and glucose and protein metabolism were measured. The expression of genes related to lipogenesis, namely, fatty acid synthase (Fasn), sterol regulatory element-binding protein 1 (Srebf1), stearoyl-CoA desaturase-1 (Scd1), and peroxisome proliferator-activated receptor gamma (Pparg) were measured by RT-PCR. PCB exposure did not alter Fasn or Srebf1 mRNA levels although the PCB126 group demonstrated a trend for decreased Fasn and Srebf1 mRNA levels (Supplementary Fig. 5), consistent with previous findings (Wahlang et al. 2017a, b). Similarly, Aroclor1260 + PCB126 exposure decreased Scd1 mRNA levels (Fig. 7a), consistent with protein abundance data (0.70-fold, p = 1.35E−02, Supplementary Table 2) and a prior study (Hardesty et al. 2019b). The same group also showed increased Pparg gene expression (Fig. 7b). With regards to lipid transport, the hepatic mRNA levels of Cd36 and fatty acid binding protein 1 (Fabp1) were determined. Both Aroclor1260 and PCB126 contributed to the upregulated Cd36 expression in the Aroclor1260 + PCB126 mixture (Fig. 7c). There was no significant difference for Fabp1 mRNA levels between groups (Supplementary Fig. 5). In terms of lipid breakdown, the mRNA levels of Pparα, a transcription factor that regulates fatty acid β-oxidation, the Aroclor1260 + PCB126 group decreased Pparα levels (Fig. 7d). These results differed from the proteomics data that showed decreased protein abundance only for Aroclor1260. PCB126 exposure also decreased the expression of the lipolytic gene, patatin-like phospholipase domain-containing protein 3 (Pnpla3, Fig. 7e). In addition, the gene expression of phosphoenolpyruvate carboxykinase 1 (Pck1), a gluconeogenic gene, was measured and found to be decreased with PCB126 exposure (Fig. 7f). Proteomics data demonstrated that PCB126 disrupted the coordination of glycolysis and gluconeogenesis by simultaneously downregulating abundance of hexokinase-3 (0.52-fold, p = 2.53E−03), phosphofructokinase (0.61-fold, p = 6.53E−03), and glucose-6-phosphatase (0.51-fold, p = 1.33E−02). The gene expression of albumin (Alb), a biomarker for protein metabolism, was increased in the Aroclor1260 + PCB126 group (Fig. 7g). Overall, these results confirm that PCBs differentially altered hepatic intermediary metabolic processes.
Discussion
PCBs, including both DL and NDL congeners, are metabolism-disrupting chemicals (Heindel et al. 2017). PCBs exposures are associated with obesity-related diseases including diabetes, dyslipidemia, cardiovascular disease, and NAFLD. PCB-related NAFLD mechanisms was recently reviewed (Wahlang et al. 2019a). In animal models fed a healthy diet, DL, but not NDL PCBs, caused hepatic steatosis and NAFLD. In contrast, NDL PCBs exposures compromised the liver, thereby increasing the histologic severity of HFD-induced NAFLD. The NDL PCB exposures variably increased diet-induced hepatic steatosis, inflammation, and fibrosis while interacting with PCB receptors including PXR, CAR, and the EGFR (Wahlang et al. 2016, 2014b, 2019b; Hardesty et al. 2019b, 2017, 2019a, 2018). While humans have simultaneous exposures to both PCB classes, no previously published studies in the literature have systematically evaluated the disease modifying effects of DL PCBs, NDL PCBs, and the more environmentally relevant combination of DL + NDL PCBs in a chronic exposure model of HFD-induced NAFLD. Few to no published data exist on either DL or DL + NDL PCBs in such a model.
Our recently published acute PCB exposure study demonstrated that Aroclor1260, PCB126, and Aroclor1260 + PCB126 exposures differentially affected histology and disease mechanisms in liver and related organs in male mice fed a healthy diet (Shi et al. 2019). Based on the direction of these results, environmental relevance, and knowledge gaps in the literature, the objective of the current study was to elucidate the effects and potential mechanisms of these different PCB exposures in a diet-induced obesity mouse model of NAFLD.
The combination of the phenotyping, histologic, and molecular biomarkers demonstrated the following differential PCB effects on metabolic conditions related to HFD-induced obesity. Consistent with prior studies (Wahlang et al. 2019a), Aroclor1260 increased liver inflammation/injury and the white adipose tissue to body weight ratio. PCB126 decreased liver inflammation and fibrosis at the molecular level. PCB126 alone did not increase hepatic free fatty acids and hepatic triglycerides, as reported previously (Shi et al. 2019). These differences are likely related to differences in duration of exposure or diet. Aroclor1260 + PCB126 modulated lipid metabolism such as hepatic free fatty acids were increased, while the plasma cholesterol/triglycerides were decreased, and it decreased liver inflammation. The lipid effects were mediated predominantly by the PCB126 component of this mixture as previously reported (Shi et al. 2019). Therefore, PCB126 co-exposure attenuated Aroclor1260-induced hepatic inflammation despite worsening the disruption of intermediary and xenobiotic metabolism. None of the exposures affected glucose tolerance or adipokines. AhR activation was highest with PCB126 alone; increased to a lesser degree with Aroclor1260 + PCB126; and absent with Aroclor1260, consistent with Shi et al. (2019). The effects on CAR were complex. Cyp2b10 gene expression was increased by Ar1260; IPF demonstrated enriched CAR interactions with PCB126; and Aroclor1260 + PCB126 induced Nr1i3 gene expression. PCBs induced Cyp2b10 gene expression to a lower degree in HFD-fed mice compared with mice fed a low fat diet (Shi et al. 2019).
Proteomics was performed to elucidate potential mechanisms for the observed differences in metabolic phenotype and TASH severity, which were greatest for Aroclor1260 versus the more similar PCB126 and Aroclor1260 + PCB126 exposure groups. The large number of differentially abundant proteins between the PCB exposure groups was surprising. For example, 99.58% of these proteins were not conserved across all three treatment groups. PCB126 and Aroclor1260 + PCB126 altered many more proteins than Aroclor1260. However, 79.06% of the proteins altered by either of the latter two treatments were not common to both groups. Despite these differences, PCB126 and Arclor1260 + PCB126 shared many top GO processes consistent with the observed similarities in liver histologic phenotype. The observed alterations in cholinergic neurotransmission associated with these exposures is novel and could be mechanistic. For example, human neonates exposed to PCBs display a number of hematologic and immunologic disturbances in neuronal signaling, immune regulation via the AhR and other receptors (Patandin et al. 1998; ten Tusscher et al. 2003). In addition, these exposures alter many normal medical biomarkers used in clinical medicine, such as cholesterol levels, AST enzyme levels, etc.
While a number of new putative PCB targets and modes of action in TASH were identified, others were reassuringly consistent with known targets. Aroclor1260 exposure was associated with the “negative regulation of receptor internalization” GO process and overconnected interactions with protein kinases including mTOR, AK3L1, p38γ, and PKCα. These findings could be consistent with the decreased EGF-stimulated EGFR internalization with consequent phosphoprotein signaling disruption previously reported for Aroclor1260-associated TASH (Hardesty et al. 2017, 2019a, b, 2018; Wahlang et al. 2019c). These studies reported decreased protein kinase B (Akt)/mTOR and extracellular signal-regulated kinases (ERK) signaling. AK3L1 is a downstream EGFR target that regulates glycolysis, thereby adding a new link between signaling disruption and intermediary metabolism (Jan et al. 2019). Intriguingly, p38γ and PKCα regulate both EGF-dependent ERK signaling (Abera and Kazanietz 2015; Lei et al. 2017; Yin et al. 2017) and NASH (Gonzalez-Teran et al. 2016; Zhou et al. 2019). The potential mechanistic roles of these kinases warrant future investigation in Aroclor1260-associated TASH.
The observed alterations in GO processes related to hepatic metal hemostasis and cytoskeleton for PCB126 could be mechanistic. PCB126-induced metal dysregulation has been described previously (Klaren et al. 2015, 2018). However, the specific effector proteins identified by the present study (e.g., hepcidin, ferritin, zinc transporter 4, etc.) and the hepcidin-regulating transcription factor, ZFPM1, (Bagu et al. 2013) warrant future investigation. Several cytoskeleton proteins were the most upregulated proteins by PCB126. The other exposures were associated with altered abundance of keratins 2, 4, 5, or 6a. Cellular localization analysis of a previous hepatic proteomics experiment from our group demonstrated that cytoskeletal-associated proteins were the top enriched cellular location for liver proteins differentially regulated by Aroclor1260 in mice fed a control diet, but this decreased in HFD-fed mice (Hardesty et al. 2019b). Circulating epithelial cell-derived keratin18 was associated with PCB-related TASH in ACHS (Clair et al. 2018). Ethanol-associated alterations of the hepatocyte cytoskeleton may influence the progression of alcohol-related steatohepatitis (Shepard and Tuma 2010). Therefore, the potential mechanistic role of PCB-induced cytoskeletal changes in TASH warrants future investigation. If the observed reduction in hepatic AST protein abundance is confirmed in humans, the utility of circulating AST enzyme activity as a biomarker for PCB-related liver toxicity in environmental epidemiology studies would be reduced. Several GO processes uniquely enriched with Aroclor1260 + PCB126 co-exposure were discovered, including the epigenetic regulation of gene expression and the localization of macromolecules including proteins.
IPF analysis of the PCB126 and Aroclor1260 + PCB126 exposure groups revealed overconnected interactions with several transcription factors regulating myriad processes involved in the resolution and repair of liver injury including inflammation, stellate cell plasticity and fibrosis, stem cells, proliferation, differentiation, metabolism, and transformation. These transcription factors are new targets for PCB hepatotoxicity in NASH and include ETS1 (Sizemore et al. 2017; Marcher et al. 2019), SOX17 (Rommelaere et al. 2014; Merino-Azpitarte et al. 2017), GABPα (Niopek et al. 2017; Sizemore et al. 2017), c-FOS (Bakiri et al. 2017), ZFX (Ding et al. 2018), N-Myc (Qin et al. 2018), and AML1 (Marcher et al. 2019). While tumors were not observed in the present study, the identification of oncogenes is not surprising because PCB126 has previously been associated with liver cancer. Although Cyp1a2 message and protein were induced by PCB126 and Aroclor1260 + PCB126, MetaCore analyses provided no evidence to support the potential AhR-dependence in any of the identified GO processes. Moreover, IPF analysis did not identify AhR as an overconnected transcription factor. While this could simply be a limitation of the software package, future studies are required to determine the AhR-dependence of PCB-related TASH.
Aroclor1260 and PCB126 did not result in either additive hepatotoxicity or additive differential protein abundances in this chronic, diet-induced NAFLD model. Hepatic protein abundance is a function of protein synthesis, degradation and transport into and out of the liver. Epigenetics-related GO processes were enriched in the Aroclor1260 + PCB126 exposure group, and this may have influenced the synthesis rates of specific hepatic proteins. The abundance of multiple epigenetics-related proteins was reduced including histone deacetylase 2; histones H1.2, H1.4, H1.5, and H1t; RNA polymerase-associated protein CTR9 homolog; RNA binding protein 3; and LIM domain-containing protein 1 and protein arginine methyltransferase 7). The proteins are involved in numerous epigenetics processes including histone acetylation, methylation, and microRNAs. IPF demonstrated overconnected interactions with the methyltransferase, PRMT1, and the several transcription factors associated with the epigenetic regulation of gene expression such as ETS1 and GABPα (Sizemore et al. 2017). The abundance of other proteins involved in epigenetics and the regulation of gene expression was altered in the other treatment groups (e.g., the noncanonical poly(A) RNA polymerase PAPD5, Supplementary Table 2) in Aroclor1260). Future studies are required to establish the mechanistic importance of epigenetic mechanisms in the genesis, progression, and potential heritability of PCB-related liver diseases.
Although perhaps the most comprehensive analysis of different chronic PCB exposures in an animal model of NAFLD, the present study is not without its limitations. Most notably, while proteins regulating metal homeostasis and epigenetics were implicated, direct measurement of hepatic metals and epigenetic signatures was not performed. While we previously published a proteomics analyses in the HFD plus Aroclor1260 TASH model (Hardesty et al. 2019b), the present experiment utilized a different, and potentially more sensitive, proteomics technique (TMT labeling) as well as different analytic procedures. This may limit the comparability of results between studies, and as a result, the MetaCore outcomes may vary. Sex differences were previously reported for Aroclor1260-induced TASH (Wahlang et al. 2019c), with female mice being more susceptible. Because male mice were utilized in the present study, potential sex differences for PCB126 and Aroclor1260 + PCB126 exposures in TASH remain unknown. While Aroclor1260 + PCB126 exposures were associated with a pancreatic pathology resembling diabetic exocrine pancreatopathy in an acute model (Hardesty et al. 2019b), the present study did not evaluate pancreatic endpoints because GTT was unchanged.
In conclusion, the present study demonstrated the complexity of the hepatic effects of PCB mixtures. DL PCBs, NDL PCBs, and a more environmentally relevant mixture of both types of PCBs differentially modulated the hepatic proteome and the severity of diet-induced NAFLD. Aroclor1260 increased hepatic inflammation and phosphoprotein signaling disruption consistent with prior research. PCB126 decreased hepatic inflammation and fibrosis at the molecular level. Mechanisms including altered cytoskeletal remodeling, metal homeostasis, and disruption of intermediary and xenobiotic metabolism were implicated in PCB126’s mode of action, and all have been previously reported. Neither the histologic nor the proteomic effects of Aroclor1260 and PCB126 were additive in the co-exposure model. PCB126 attenuated Aroclor1260-induced hepatic inflammation but increased hepatic free fatty acids while reducing plasma lipids. The overwhelming majority of differentially regulated hepatic proteins associated with Aroclor1260 + PCB126 exposure were not associated with either Aroclor1260 or PCB1260 exposure. Likewise, most proteins associated with either Aroclor1260 or PCB126 exposures were not associated with co-exposure to both. Despite this, many of the top GO processes and overconnected interactions by protein function were common to the PCB126 and Aroclor1260 + PCB126 exposure groups. These conserved processes were broadly related to metabolism and cellular organization and localization. A complex web of overconnected transcription factors broadly regulating metabolism; liver injury and inflammation; and liver repair was identified. Aroclor1260 + PCB126 exposure was strongly associated with multiple epigenetic processes, and these could potentially explain the observed nonadditive effects of the exposures on the hepatic proteome. When compared with our recently published acute study investigating the same PCB exposures, the modifying effect on diet (and possibly duration) on PCB-induced hepatotoxicity is also demonstrated. More data including translational research are required on potential modes of PCB action in NAFLD including phosphoprotein signaling disruption; abnormal metal homeostasis; cytoskeletal remodeling; and transcriptional reprogramming by epigenetic mechanisms. Likewise, potential sex differences and the AhR-dependence of PCB126’s NAFLD modifying effects warrant future investigation.
Abbreviations
- ACHS:
-
Anniston Community Health Survey
- AhR:
-
Aryl hydrocarbon receptor
- AK3L1:
-
Adenylate kinase isoenzyme
- Alb:
-
Albumin
- ALT:
-
Alanine transaminase
- AML1:
-
Acute myeloid leukemia 1
- AST:
-
Aspartate transaminase
- BW:
-
Body weight
- CAE:
-
Chloroacetate esterase
- CAR:
-
Constitutive androstane receptor
- Cd68:
-
Cluster of differentiation 68
- c-FOS:
-
Cellular oncogene FOS
- Cyp:
-
Cytochrome P450
- DL:
-
Dioxin-like
- EGFR:
-
Epidermal growth factor receptor
- EPF:
-
Enrichment by protein function
- ERK:
-
Extracellular signal-regulated kinases
- ETS1:
-
Protein C-ets-1
- Fabp1:
-
Fatty acid binding protein 1
- Fasn:
-
Fatty acid synthase
- GABPα:
-
GA binding protein transcription factor subunit α
- GCR:
-
Glucocorticoid receptor
- GO:
-
Gene ontology
- H&E:
-
Hematoxylin–eosin
- HFD:
-
High-fat diet
- IPF:
-
Interaction by protein function
- KNG1:
-
Kininogen 1
- LW:
-
Liver weight
- mTOR:
-
Mammalian target of rapamycin
- NAFLD:
-
Nonalcoholic fatty liver disease
- NASH:
-
Nonalcoholic steatohepatitis
- NDL:
-
Non-dioxin-like
- NHANES:
-
National Health and Nutrition Examination Survey
- N-Myc:
-
Neuroblastoma MYC oncogene
- p38γ:
-
Alternate mitogen-activated protein kinase
- PCBs:
-
Polychlorinated biphenyls
- Pck1:
-
Phosphoenolpyruvate carboxykinase 1
- PKCα:
-
Protein kinase C α
- Pnpla3:
-
Patatin-like phospholipase domain-containing protein 3
- Ppara:
-
Peroxisome proliferator-activated receptor alpha
- Pparg:
-
Peroxisome proliferator-activated receptor gamma
- PRMT1:
-
Protein arginine methyltransferase 1
- PXR:
-
Pregnane-xenobiotic receptor
- SART1:
-
Hypoxia-associated factor
- Scd1:
-
Stearoyl-CoA desaturase-1
- SOX17:
-
SRY-box transcription factor 17
- Srebf1:
-
Sterol regulatory element-binding protein 1
- TASH:
-
Toxicant-associated steatohepatitis
- TCDD:
-
Tetrachlorodibenzo-p-dioxin
- TMT:
-
Tandem mass tag
- ZFPM1:
-
Zinc finger protein
- FOG:
-
Family member 1
- ZFX:
-
Zinc finger protein x-linked
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Acknowledgements
This work was funded, in part, by the National Institutes of Health (R35ES028373 (MCC), P42ES023716 (MCC), T32ES011564 (BW), P20GM113226 (MCC), and P50AA024337 (MCC)). The authors would like to acknowledge: (i) the Omics Core of UofL’s Hepatobiology and Toxicology Center, Daniel W. Wilkey and Frederick W. Benz for proteomics assistance; (ii) the Hepatobiology and Toxicology Center’s Animal Model and Biorepository Core for the support required to develop and analyze the novel animal exposure models utilized in this manuscript; (iii) the Sample and Data Management Core of UofL’s Superfund Research Center; and (iv) Linda S. Birnbaum, PhD, whose idea it was to spike PCB126 into the Aroclor1260 thereby creating a potentially more environmentally relevant PCB mixture for use in experimental studies.
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Jin, J., Wahlang, B., Shi, H. et al. Dioxin-like and non-dioxin-like PCBs differentially regulate the hepatic proteome and modify diet-induced nonalcoholic fatty liver disease severity. Med Chem Res 29, 1247–1263 (2020). https://doi.org/10.1007/s00044-020-02581-w
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DOI: https://doi.org/10.1007/s00044-020-02581-w