Abstract
Watermelon (Citrullus lanatus) belongs to the family Cucurbitaceae. The crop is grown commercially in regions with extended warm, frost-free months. Watermelon is cultivated for its colorful, tender, juicy, and sweet fruit. They are generally consumed fresh and make an excellent and delicious dessert, particularly during the summer months. Because of its smaller genome and large number of gene mutations, watermelon is a suitable crop species for genetic research. Watermelon’s genome has 424 million base pairs. DNA sequencing found significant conservation, which is relevant for comparative genomics within Cucurbitaceae and other species. There exists a huge genetic variability in the fruit quality characteristics of watermelon with respect to seed traits, fruit shape, fruit size, skin color, skin pattern, flesh color and sugar/acid composition, fruit bitterness, and many more. This chapter serves as a guide to show the prospects and advances made in the genetics of fruit qualitative traits in watermelon breeding programs depending on profitability and consumer preferences.
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References
Anees M, Gao L, Umer MJ, Yuan P, Zhu H, Lu X, He N, Gong C, Kaseb MO, Zhao S, Liu W (2021) Identification of key gene networks associated with cell wall components leading to flesh firmness in watermelon. Front Plant Sci 12:1164. https://doi.org/10.3389/FPLS.2021.630243/BIBTEX
Bahri S, Zerrouk N, Aussel C, Moinard C, Crenn P, Curis E, Chaumeil J-C, Cynober L, Sfar S (2013) Citrulline: from metabolism to therapeutic use. Nutrition 29:479–484
Branham S, Vexler L, Meir A, Tzuri G, Frieman Z, Levi A, Wechter WP, Tadmor Y, Gur A (2017) Genetic mapping of a major codominant QTL associated with β-carotene accumulation in watermelon. Mol Breed 37:1–13. https://doi.org/10.1007/S11032-017-0747-0
Chambliss OL, Erickson HT, Jones CM (1968) Genetic control of bitterness in watermelon fruits. In: Proceedings of the American Society for Horticultural Science. American Society for Horticultural Science, Alexandria, VA, USA, pp 539–546
Chomicki G, Renner SS (2015) Watermelon origin solved with molecular phylogenetics including Linnaean material: another example of museomics. New Phytol 205:526–532
Dane F, Lang P (2004) Comparative analysis of cpDNA variability in wild and cultivated Citrullus species: implications for evolution of watermelon. Am J Bot 91:1922–1929
Dou J, Zhao S, Lu X, He N, Zhang L, Ali A, Kuang H, Liu W (2018) Genetic mapping reveals a candidate gene (ClFS1) for fruit shape in watermelon (Citrullus lanatus L.). Theor Appl Genet 131:947–958. https://doi.org/10.1007/S00122-018-3050-5
Everts S (2016) Bitterness in wild cucumber, melon, and watermelon. Chem Eng News 94(48):10–11
Fall LA, Perkins-Veazie P, Ma G, McGregor C (2019) QTLs associated with flesh quality traits in an elite× elite watermelon population. Euphytica 215:1–14
Fan M, Xu Y, Zhang HY, Ren HZ, Kang GB, Wang YJ, Chen H (2000) Identification of quantitative trait loci associated with fruit traits in watermelon [Citrullus lanatus (Thunb) Mansf] and analysis of their genetic effects. Yi Chuan Xue Bao 27:902–910
Fang X, Liu S, Gao P, Liu H, Wang X, Luan F, Zhang Q, Dai Z (2020) Expression of ClPAP and ClPSY1 in watermelon correlates with chromoplast differentiation, carotenoid accumulation, and flesh color formation. Sci Hortic (Amsterdam) 270:109437. https://doi.org/10.1016/J.SCIENTA.2020.109437
Gao L, Zhao SJ, Lu XQ, He N, Zhu HJ, Dou JL (2016) Linkage analysis on flesh firmness of watermelon [Citrullus lanatus (Thunb.) Mansf.] by SSR molecular marker. J Plant Genet Resour 17:866–870
Gao L, Zhao S, Lu X, He N, Liu W (2018) ‘SW’, a new watermelon cultivar with a sweet and sour flavor. HortScience 53:895–896
Garcia-Lozano M, Dutta SK, Natarajan P, Tomason YR, Lopez C, Katam R, Levi A, Nimmakayala P, Reddy UK (2019) Transcriptome changes in reciprocal grafts involving watermelon and bottle gourd reveal molecular mechanisms involved in increase of the fruit size, rind toughness and soluble solids. Plant Mol Biol 102:213–223. https://doi.org/10.1007/S11103-019-00942-7
Gong C, Li B, Anees M, Zhu H, Zhao S, He N, Lu X, Liu W (2022) Fine-mapping reveals that the bHLH gene Cla011508 regulates the bitterness of watermelon fruit. Sci Hortic (Amsterdam) 292:110626. https://doi.org/10.1016/J.SCIENTA.2021.110626
Guner N, Wehner TC (2004) The genes of watermelon. HortScience 39:1175–1182. https://doi.org/10.21273/HORTSCI.39.6.1175
Guo S, Zhang J, Sun H, Salse J, Lucas WJ, Zhang H, Zheng Y, Mao L, Ren Y, Wang Z (2013) The draft genome of watermelon (Citrullus lanatus) and resequencing of 20 diverse accessions. Nat Genet 45:51–58
Guo S, Sun H, Zhang H, Liu J, Ren Y, Gong G, Jiao C, Zheng Y, Yang W, Fei Z, Xu Y (2015) Comparative transcriptome analysis of cultivated and wild watermelon during fruit development. PLoS One 10:e0130267. https://doi.org/10.1371/JOURNAL.PONE.0130267
Gusmini G, Wehner TC (2006) Qualitative inheritance of rind pattern and flesh color in watermelon. J Hered 97:177–185. https://doi.org/10.1093/JHERED/ESJ023
Hashizume T, Shimamoto I, Hirai M (2003) Construction of a linkage map and QTL analysis of horticultural traits for watermelon [Citrullus lanatus (THUNB.) MATSUM & NAKAI] using RAPD, RFLP and ISSR markers. Theor Appl Genet 106:779–785
Joshi V, Joshi M, Silwal D, Noonan K, Rodriguez S, Penalosa A (2019) Systematized biosynthesis and catabolism regulate citrulline accumulation in watermelon. Phytochemistry 162:129–140
Kim YC, Choi D, Zhang C, Liu H-f, Lee S (2018) Profiling cucurbitacins from diverse watermelons (Citrullus spp.). Hortic Environ Biotechnol 59:557–566. https://doi.org/10.1007/S13580-018-0066-3
Levi A, Jarret R, Kousik S, Patrick Wechter W, Nimmakayala P, Reddy UK (2017) Genetic resources of watermelon. In: Genetics and genomics of Cucurbitaceae. Springer, Cham, pp 87–110
Li B, Lu X, Dou J, Aslam A, Gao L, Zhao S, He N, Liu W (2018) Construction of a high-density genetic map and mapping of fruit traits in watermelon (Citrullus Lanatus L.) based on whole-genome resequencing. Int J Mol Sci 19:3268. https://doi.org/10.3390/IJMS19103268
Liao N, Hu Z, Li Y, Hao J, Chen S, Xue Q, Ma Y, Zhang K, Mahmoud A, Ali A, Malangisha GK, Lyu X, Yang J, Zhang M (2020) Ethylene-responsive factor 4 is associated with the desirable rind hardness trait conferring cracking resistance in fresh fruits of watermelon. Plant Biotechnol J 18:1066–1077. https://doi.org/10.1111/PBI.13276
Lou L, Wehner TC (2016) Qualitative inheritance of external fruit traits in watermelon. HortScience 51:487–496
Lu BY, Zhou HW, Chen X, Luan FS, Wang XZ, Jiang Y (2016) QTL analysis of fruit traits in watermelon. J Fruit Sci 33:1206–1218
Lv P, Li N, Liu H, Gu H, Zhao WE (2015) Changes in carotenoid profiles and in the expression pattern of the genes in carotenoid metabolisms during fruit development and ripening in four watermelon cultivars. Food Chem 174:52–59. https://doi.org/10.1016/J.FOODCHEM.2014.11.022
Mashilo J, Shimelis H, Ngwepe RM, Thungo Z (2022) Genetic analysis of fruit quality traits in sweet watermelon (Citrullus lanatus var. lanatus): a review. Front Plant Sci 13. https://doi.org/10.3389/FPLS.2022.834696
Matsuo K, DeMilo AB, Schroder RFW, Martin PAW (1999) Rapid high-performance liquid chromatography method to quantitate elaterinide in juice and reconstituted residues from a bitter mutant of Hawkesbury watermelon. J Agric Food Chem 47:2755–2759. https://doi.org/10.1021/JF9811572
Navot N, Sarfatti M, Zamir D (1990) Linkage relationships of genes affecting bitterness and flesh color in watermelon. J Hered 81:162–165. https://doi.org/10.1093/OXFORDJOURNALS.JHERED.A110952
Park S-w, Kim KT, Kang SC, Yang HB (2016) Rapid and practical molecular marker development for rind traits in watermelon. Hortic Environ Biotechnol 57:385–391. https://doi.org/10.1007/S13580-016-0005-0
Pei S, Liu Z, Wang X, Luan F, Dai Z, Yang Z, Zhang Q, Liu S (2021) Quantitative trait loci and candidate genes responsible for pale green flesh colour in watermelon (Citrullus lanatus). Plant Breed 140:349–359. https://doi.org/10.1111/PBR.12908
Poole CF, Grimball PC (1945) Interaction of sex, shape, and weight genes in watermelon. J Agric Res 71:533–552
Ren Y, Li M, Guo S, Sun H, Zhao J, Zhang J, Liu G, He H, Tian S, Yu Y (2021) Evolutionary gain of oligosaccharide hydrolysis and sugar transport enhanced carbohydrate partitioning in sweet watermelon fruits. Plant Cell 33:1554–1573
Robinson RW, Munger HM, Whitaker TW, Bohn GW (1976) Genes of the Cucurbitaceae. Hortic Sci 11:554–568
Sun T, Yuan H, Cao H, Yazdani M, Tadmor Y, Li L (2018) Carotenoid metabolism in plants: the role of plastids. Mol Plant 11:58–74. https://doi.org/10.1016/J.MOLP.2017.09.010
Sun L, Zhang Y, Cui H, Zhang L, Sha T, Wang C, Fan C, Luan F, Wang X (2020) Linkage mapping and comparative transcriptome analysis of firmness in watermelon (Citrullus lanatus). Front Plant Sci 11:831. https://doi.org/10.3389/FPLS.2020.00831/BIBTEX
Tanaka T, Wimol S, Mizutani T (1995) Inheritance of fruit shape and seed size of watermelon. J Jpn Soc Hortic Sci 64:543–548. https://doi.org/10.2503/JJSHS.64.543
Umer MJ, Bin Safdar L, Gebremeskel H, Zhao S, Yuan P, Zhu H, Kaseb MO, Anees M, Lu X, He N, Gong C, Liu W (2020a) Identification of key gene networks controlling organic acid and sugar metabolism during watermelon fruit development by integrating metabolic phenotypes and gene expression profiles. Hortic Res 7. https://doi.org/10.1038/S41438-020-00416-8/41991606/41438_2020_ARTICLE_416.PDF
Umer MJ, Gao L, Gebremeskel H, Bin SL, Yuan P, Zhao S, Xuqiang L, Nan H, Hongju Z, Liu W (2020b) Expression pattern of sugars and organic acids regulatory genes during watermelon fruit development. Sci Hortic (Amsterdam) 265:109102. https://doi.org/10.1016/j.scienta.2019.109102
Van Der Knaap E, Tanksley SD (2001) Identification and characterization of a novel locus controlling early fruit development in tomato. Theor Appl Genet 103:353–358. https://doi.org/10.1007/S001220100623
Wang Y, Guo S, Tian S, Zhang J, Ren Y, Sun H, Gong G, Zhang H, Xu Y (2017) Abscisic acid pathway involved in the regulation of watermelon fruit ripening and quality trait evolution. PLoS One 12:e0179944. https://doi.org/10.1371/JOURNAL.PONE.0179944
Wang Y, Wang J, Guo S, Tian S, Zhang J, Ren Y, Li M, Gong G, Zhang H, Xu Y (2021) CRISPR/Cas9-mediated mutagenesis of ClBG1 decreased seed size and promoted seed germination in watermelon. Hortic Res 8. https://doi.org/10.1038/S41438-021-00506-1/42043543/41438_2021_ARTICLE_506.PDF
Wechter WP, Levi A, Harris KR, Davis AR, Fei Z, Katzir N, Giovannoni JJ, Salman-Minkov A, Hernandez A, Thimmapuram J, Tadmor Y, Portnoy V, Trebitsh T (2008) Gene expression in developing watermelon fruit. BMC Genomics 9:1–13. https://doi.org/10.1186/1471-2164-9-275/FIGURES/5
Yao Y-X, Li M, Zhai H, You C-X, Hao Y-J (2011) Isolation and characterization of an apple cytosolic malate dehydrogenase gene reveal its function in malate synthesis. J Plant Physiol 168:474–480
Zhu Q, Gao P, Liu S, Zhu Z, Amanullah S, Davis AR, Luan F (2017) Comparative transcriptome analysis of two contrasting watermelon genotypes during fruit development and ripening. BMC Genomics 18:1–20
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Dutta, S.K., Nimmakayala, P., Reddy, U.K. (2023). Watermelon: Advances in Genetics of Fruit Qualitative Traits. In: Kole, C. (eds) Compendium of Crop Genome Designing for Nutraceuticals. Springer, Singapore. https://doi.org/10.1007/978-981-19-3627-2_36-1
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DOI: https://doi.org/10.1007/978-981-19-3627-2_36-1
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