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Microscopy Using Liquid Lenses for Industrial and Biological Applications

Error analysis and uncertainty evaluation

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Handbook of Metrology and Applications

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Abstract

Quantitative Microscopy refers to the surface metrology of microscopic structures. It deals with the interaction of light with material and quantifies the changes in terms of surface measurements. This chapter describes optical metrology techniques used in qualitative and quantitative microscopy and explains the different extended depth of focus techniques used in quantitative microscopy. The prime focus is on the usage of liquid lenses as a potential solution for extended depth of focus imaging in quantitative microscopy. This chapter summarizes a number of applications of liquid lenses in microscopy dealing with the scope in industry and biological sciences. In the last section, the concept of uncertainty of measurement along with the uncertainty budget has been explained taking into consideration the relevant factors responsible for uncertainty.

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Acknowledgment

Vineeta Kumari and Gyanendra Sheoran gratefully acknowledge the support of Department of Science and Technology (DST) under grant number DST/TDT/SHRI-07/2018.

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Authors and Affiliations

  1. Maharaja Surajmal Institute of Technology, Delhi, India

    Neelam Barak

  2. National Institute of Technology Delhi, Delhi, India

    Vineeta Kumari & Gyanendra Sheoran

Authors
  1. Neelam Barak
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  2. Vineeta Kumari
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  3. Gyanendra Sheoran
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Corresponding author

Correspondence to Gyanendra Sheoran .

Editor information

Editors and Affiliations

  1. Bhabha Atomic Research Centre, Mumbai, Maharashtra, India

    Dinesh K. Aswal

  2. Physico-Mechanical Metrology, CSIR - National Physical Laboratory, New Delhi, Delhi, India

    Sanjay Yadav

  3. National Metrology Institute of Japan, National Institute of Advanced Industria, Ibaraki, Japan

    Toshiyuki Takatsuji

  4. National Institute of Standards and Tech, GAITHERSBURG, MD, USA

    Prem Rachakonda

  5. Mechanical Engineering Department, National Institute of Technology, Delhi, India

    Harish Kumar

Section Editor information

  1. Length, Dimension and nanometrogy, CSIR-National Physical Laboratory, New Delhi, India

    Arif sanjid MAHAMMAD PhD

  2. Maharaja Surajmal Institute Of Technology, New Delhi, India

    K. P. Chaudhary PhD

  3. C.V. Raman Global University, Bangalore, India

    B. S. Satyanarayan PhD

  4. National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan

    Toshiyuki Takatsuji

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Barak, N., Kumari, V., Sheoran, G. (2022). Microscopy Using Liquid Lenses for Industrial and Biological Applications. In: Aswal, D.K., Yadav, S., Takatsuji, T., Rachakonda, P., Kumar, H. (eds) Handbook of Metrology and Applications. Springer, Singapore. https://doi.org/10.1007/978-981-19-1550-5_77-1

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  • DOI: https://doi.org/10.1007/978-981-19-1550-5_77-1

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  • Print ISBN: 978-981-19-1550-5

  • Online ISBN: 978-981-19-1550-5

  • eBook Packages: Springer Reference EngineeringReference Module Computer Science and Engineering

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