Abstract
CEUS in the portal venous and late phase after injection of SonoVue® considerably improves the characterization of liver tumors compared with conventional B mode ultrasound, leading to possible preoperative differentiation of malignant liver lesions from benign ones. In this chapter, we discussed contrast enhanced ultrasound features of some common malignant focal liver lesions, including hepatocellular carcinoma, intrahepatic cholangiocarcinoma, liver metastasis, as well as dysplasia nodulars in liver cirrhosis.
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Keywords
- Contrast enhanced ultrasound; Liver; Malignancy; Characterization; Detection; Differential diagnosis
4.1 Hepatocellular Carcinoma
4.1.1 Introduction
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Hepatocellular carcinoma (HCC) is the most common primary liver malignant tumors and a major cause of morbidity and death worldwide. Currently, it is the second leading cause of cancer-related death [1]. It is the sixth most common cancer and the fourth cause of cancer death, with a 5-year survival rate of 18%. It is estimated that in 2030 more than 1 million patients will die of liver cancer [2].
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HCC develops in various chronic liver disease backgrounds: chronic hepatitis caused by hepatitis B or C virus (HBV or HCV), nonalcoholic or alcoholic steatohepatitis, etc.
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HCC develops in a multistep fashion. Various pre-cancerous lesions, including regenerative nodules (RN), and dysplastic nodules (DN), progress to early HCCs, well differentiated, moderately differentiated, or poorly differentiated HCCs (Fig. 4.1).
4.1.2 Indications of Liver CEUS
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For all focal liver lesions detected during routine ultrasound or surveillance in the cirrhotic or noncirrhotic liver, CEUS is indicated to characterize and to establish a diagnosis of HCC (followed by CT and MRI for staging).
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To characterize focal liver lesions not suitable for biopsy, especially those remained indeterminate after CT or MR examinations.
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For patients with multiple focal liver lesions, CEUS is recommended to select a nodule or nodules with different contrast enhancement patterns for biopsy.
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CEUS is used to reach further diagnosis in patients with inconclusive histologic or cytologic results.
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To monitor dynamic changes in contrast enhancement patterns over time for follow up of suspected malignant hepatocellular lesions.
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Based on contrast enhancement, to differentiate tumor in vein from bland thrombus in vein.
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To monitor and target ablation therapy response of HCC during and after ablation or chemotherapy treatments.
4.1.3 CEUS Features of HCC
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More than 95% of focal liver lesions in liver cirrhosis are HCCs.
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Typical contrast enhanced ultrasound features of HCCs including: hyperenhancement in the early arterial phase, typically mild wash-out in the late phase. For HCC lesions, mild wash-out most often does not occur until very late in the late phase (>3 min) (Figs. 4.2 and 4.3).
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Well-differentiated HCCs may lack wash-out in the late phase. In cirrhotic liver, only arterial phase hyperenhancement might be sufficient for the preoperative diagnosis of HCC [3] (Figs. 4.4 and 4.5).
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On the condition that the lesion did not show wash-out after 3 min, a further observation after 4–6 min is necessary.
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A second contrast agents injection can be repeated and focuses on the wash-out area 7–10 min later after first injection, for lesions located at difficult place, or small lesion sizes which arterial phase hyperenhancement is missed, or unsatisfied imaging due to patient being unable to hold breath, and for extra findings of an indeterminate wash-out nodule in the portal venous or late phase.
4.1.4 Additional Benefit for CEUS vs CT or MRI
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CT/MRI shows wash-out of focal liver lesions in the portal venous or late phase, but does not show definite arterial phase enhancement of the lesion.
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CT/MRI shows arterial phase hyperenhancement but does not show definite wash-out.
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Depending on its unique advantages of real-time scan and continuous acquisition, CEUS can provide continuous evaluation of wash-in and wash-out process for 5 min.
4.1.5 Limitations of Liver CEUS
Some common limitations with B mode ultrasound also exist in CEUS, such as subdiaphragmatic or deep located lesions, limited penetration in large size patients, infrequent interference of bowel or gastric gas, and lesion located in severe hepatic steatosis.
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Nodules located deeper than 10 cm from the skin surface. On those conditions, sound beam attenuation will cause excess subcutaneous or hepatic fat not well depicted with CEUS.
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In some patients, the upper right subdiaphragmatic regions of the liver cannot be visualized clearly through a proper acoustic window.
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Focal liver lesions with a diameter smaller than 10 mm, especially located in heterogeneous cirrhotic liver.
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Patients who could not cooperate satisfactorily during CEUS scan (Table 4.1).
4.1.6 Surveillance
Ultrasound is a widely accepted cost-effective first-line surveillance modality for the diagnosis of HCC. It is recommended by major international liver societies, including the European Association for the Study of the Liver (EASL) and the American Association for the Study of Liver Diseases (AASLD). During surveillance of HCC, ultrasound with a-fetoprotein (AFP) has been regarded as a standard surveillance tool. With the application of contrast enhanced ultrasound, the suboptimal detection rates and false referral rates of ultrasound have been overcome (Figs. 4.6 and 4.7).
Contrast enhanced ultrasound has not been recommended for surveillance of HCC because it does not enable examination of the entire liver. Although the detection rate for early-stage HCC was not significantly improved by adding CEUS, the false positive rate was significantly reduced [4]. The contrast agent Sonzaoid® is phagocytized by liver-specific macrophages including Kupffer cells. Since Kupffer cells may be less or even absent in HCC, hypoenhancement in the postvascular phase is a highly sensitive imaging feature of HCC [5,6,7,8]. CEUS may be used as a second-line imaging tool for a lesion suspicious for HCC, which was detected with B mode ultrasound surveillance.
Contrast enhanced ultrasound scan in the delayed phase permits detection of HCC lesions. However, CEUS is not recommended for surveillance. The entire liver cannot be scanned by contrast enhanced ultrasound [9]. Instead, CEUS permits the characterization of one or several identified nodules.
4.1.7 CEUS LI-RADS
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To make accurate characterization of focal liver lesions in the cirrhotic liver is among one of the most challenging imaging problems.
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The use of a microbubble-based contrast enhanced ultrasound and the implementation of the Liver Imaging Reporting and Data System (LI-RADS) for CEUS will greatly promote the future use of CEUS in patients who are at high risk for HCC.
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The LI-RADS uses a diagnostic algorithm to assign categories and reflect the relative probability of HCC. It was created with the aim to standardize liver imaging in patients at high risk for HCC.
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CEUS LI-RADS could improve the integration of CEUS into the clinical multimodality approach for the diagnosis and treatment of the liver at risk for HCC, providing additional important information to that obtained with CT and MRI (Table 4.2).
CEUS LI-RADS includes five diagnostic categories: LR-1 (definitely benign), LR-2 (probably benign), LR-3 (intermediate malignancy probability) (Fig. 4.8), LR-4 (probably HCC) (Fig. 4.9), and LR-5 (definitely HCC) (Figs. 4.10, 4.11, and 4.12).
LR-NC (not categorized due to image degradation), LR-TIV (tumor in vein) (Figs. 4.13, 4.14, and 4.15) and LR-M (probably or definitely malignant but not HCC specific) diagnostic categories are also included in CEUS LI-RADS. CEUS has high sensitivity and accuracy in the detection and correct differentiation of a tumor thrombus from a bland thrombus [10]. CEUS LR-TIV should lead to alternative imaging assessment or repeat imaging, biopsy, or treatment.
Clinically HCCs can invade surrounding liver vessels with a prevalence of up to 20%. Portal vein thrombosis is a common imaging finding in liver cirrhosis. Differentiation between tumor thrombus and bland portal vein thrombosis is crucial for further therapeutic management. During CEUS no contrast uptake is seen in bland portal vein thrombosis inside the lumen of the portal vein. However, contrast enhancement of the thrombus indicates tumor thrombus. In the case of HCC invading the portal vein, the tumor thrombus typically shows arterial phase hyperenhancement during arterial phase and mild wash-out in the late phase.
In addition to major CEUS imaging features, ancillary features (AFs) are used to modify the likelihood that an observation is HCC [11]. Some similar phenomena described on CE-CT and CE-MRI can also be seen on CEUS, including pseudomasses, capsule vessels, and arterioportal shunts. The 2018 version of the LI-RADS guidelines provided new rules regarding the application of AFs. AFs should only be used if their presence is unequivocal; they should not be utilized if their presence is uncertain. Three CEUS AFs favor malignancy, among which, “definite growth” favors malignancy in general, nodule-in-nodule appearance (Figs. 4.16 and 4.17) and mosaic appearance (Figs. 4.18 and 4.19) favor HCC in particular [11]. These Afs may be applied to upgrade the observation by one category to a maximum classification of LR-4. They cannot be used to upgrade the category to LR-5, and their absence should not be used to downgrade the LR category [11].
4.1.8 Small HCC
The diagnostic algorithm of the international societies identifies 1 cm size as the starting point for radiological diagnosis of HCC. However, the current APASL algorithm based on the dynamic pattern of contrast imaging suggests diagnosis of HCC independently on tumor size. The reported specificity and sensitivity of CEUS in the diagnosis of HCC ≤2 cm in a cirrhotic liver were 87% and 100%, respectively [12] (Figs. 4.20 and 4.21).
4.1.9 Treatment Response Follow Up
4.1.9.1 Ablation Therapy
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In ablative therapy of liver tumors, the clinical application of CEUS including selection of patient, intraprocedural real-time guidance, and posttreatment immediate assessment. CEUS makes it possible to detect residual tumor vascularity immediately after ablation, to make additional treatments, and to increase ablation efficacy finally.
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The reactive inflammatory changes as peripheral hyperemia of the treated lesion may show rim hyperenhancement, which may last for 2–3 weeks after local ablation therapy. It is always necessary to distinguish the reactive hyperemia from residual or recurrent tumor. Comparison with pretreatment CEUS images is helpful in these patients (Fig. 4.22).
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CEUS is sensitive and accurate in detecting residual or recurrent tumors after ablation. The residual tumor demonstrates the typical hyperenhancement pattern as HCC, which is characterized by arterial phase hyperenhancement with late mild wash-out (Fig. 4.23).
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CEUS can be used to characterize the recurrence after treatment. Any new nodular demonstrating arterial hyperenhancement during CEUS, regardless of wash-out or not, is highly suspicious for HCC recurrence (Fig. 4.24).
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In addition, during the regular surveillance after ablative therapy, CEUS could be used to scan the entire liver for detection and evaluation of new nodules. During follow-up procedure, arterial phase hyperenhancement is the diagnostic characteristic of recurrence HCC on CEUS.
4.1.9.2 Transarterial Chemoembolization
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A significant proportion of HCC patients are diagnosed at intermediate or late stage. For those patients, transarterial chemoembolization (TACE) is the most widely used first-line therapy.
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The accurate evaluation of local tumor responses to the first TACE session has an important clinical impact on the overall clinical management of HCC patients.
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Currently, serological biomarkers and magnetic resonance imaging (MRI) are main methods for the prediction of HCC responses to TACE.
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CEUS has distinguished advantages in assessing treatment response after TACE. It could assess treatment response as early as 1 day after the TACE procedure. CEUS also allows assessment of microvascular perfusion changes of residual tumor (Fig. 4.25).
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CEUS has been proved to have equivalent or superior efficacy for assessing the treatment response of HCC to TACE. It makes short-time follow-up possible (Figs. 4.26 and 4.27).
4.1.9.3 Targeted Therapy
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In 2006, sorafenib (Nexavar®, Bayer AG, Germany), a multikinase inhibitor, was approved for the treatment of patients with advanced HCC. Two major angiogenesis pathways inhibited by Sorafenib were vascular endothelial growth factor receptor 2 (VEGFR2) and platelet-derived growth factor receptor (PDGFR).
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Sorafenib has been regarded as the first-line treatment for patients with advanced HCC.
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The Response Evaluation Criteria in Cancer of the Liver (RECICL) criteria to address therapeutic response in liver malignancies also puts an emphasis on the detection and measurement of necrosis. However, all of these parameters can only partially reflect the early tumor response to treatment.
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It is possible to make specific and precise evaluations for early prediction of tumor response in HCC patients by CEUS. Also, CEUS could be an excellent tool for selecting potential patients for targeted therapy.
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The unique advantages of CEUS in evaluating antitumor treatment including low cost, rapid, easy to repeat, no radiation or side effects. It is well accepted by patients.
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Time intensity curves and quantitative evaluation of dynamic CEUS parameters may provide additional information in evaluations of the treatment effects of anti-angiogenesis treatment. TTP may be an early and sensitive parameter to evaluate response to sorafenib treatment [13].
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Three-dimensional CEUS (3D-CEUS) has recently been successfully used to make accurate and clear depiction of the tumor microvascular perfusion.
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A correlation has been proved to exist between 3D-CEUS parameters and pathological changes in HCC after treatment.
4.2 Intrahepatic Cholangiocarcinoma
4.2.1 Definitions
Intrahepatic cholangiocarcinoma (ICC) is the second most common primary hepatic malignant tumor after hepatocellular carcinoma (HCC). ICC is biliary epithelial differentiation and occurs in intrahepatic biliary tree, from the segmental ducts to the smallest bile ducts. ICC could be classified into three types: mass-forming type, intraductal-growth type, and periductal infiltrating type. Mass-forming type is the most common type of ICC [14, 15].
4.2.2 Imaging
4.2.2.1 Conventional Ultrasound Findings
Mass-forming type
The mass-forming type of ICC is an expansile mass lesion with ill-defined border in the hepatic parenchyma (Fig. 4.28).
Intraductal-growth type
The intraductal-growth type of ICC is confined within the dilated part of an intrahepatic large bile duct. Tumor commonly shows a polypoid or papillary mural nodule within the dilated bile duct lumen (Figs. 4.29 and 4.30). Sometimes mild extension beyond bile duct walls can be seen. Ultrasound can identify marked, localized dilatation of affected ducts.
Periductal-infiltrating type
The periductal-infiltrating type of ICC develops along the intrahepatic bile ducts and appears as a solid mass extending along portal tracts. As a result of tumoral obstruction, the upstream ducts occur secondary dilatation (Fig. 4.31). The anatomical location of the involved ducts can be evaluated by caliber changes or ducts rigidity. This type is hard to be detected by ultrasound before surgery.
These three types may overlap in the same case. Advanced ICC often has a mixed pattern of growth with central necrosis and/or intrahepatic metastases. Ultrasound can easily detect secondary dilated bile ducts around the tumor [16,17,18].
4.2.2.2 Contrast Enhanced Ultrasound Findings
Mass-forming type
ICCs with mass-forming type have a variety of patterns in the arterial phase on contrast enhanced ultrasound (CEUS). Peripheral rim-like hyperenhancement in the arterial phase is the typical pattern (Fig. 4.32) [19]. This typical pattern could be observed in 38–69% of ICCs. The peripheral hyperenhancement areas always indicate abundant tumor cells and the central areas correspond to fibrotic stroma at pathological examination. The other enhancement patterns are heterogeneously branched hyperenhancement (Fig. 4.28), hyperenhancement with vague boundary (Fig. 4.33), and heterogeneous hypoenhancement (Fig. 4.34). In patients with cirrhosis or small tumor size, the wash-in patterns of ICC on CEUS could be similar to those of HCC, which manifest homogeneous and heterogeneous hyperenhancement. Recent studies further revealed that wash-out of ICC was much earlier (<60 s) than that of HCC, and wash-out degree was more marked in ICC than that in HCC [20,21,22,23,24].
Intraductal-growth type
ICCs with intraductal-growth type often show that the intraductal tumor manifests slightly hyperenhancement in the arterial phase and gradually enhancement, followed by portal venous/late phase wash-out (Figs. 4.29 and 4.30).
Periductal-infiltrating type
ICCs with periductal-infiltrating types are rare. It is hard to detect tumors with this type before surgery. Fibrosis is more commonly found in this type, therefore this type often shows slightly hyperenhancement or isoenhancement in the arterial phase followed by early wash-out (Fig. 4.31).
4.2.2.3 CT Findings
ICCs usually manifest to be hypodense hepatic masses with irregular margins on unenhanced CT. On arterial phase of dynamic enhanced CT, ICCs usually show peripheral enhancement, and the center of the tumor (with few cells and abundant stroma) remains poorly enhanced. The center of the tumor gradually enhances on late phase (delayed enhancement). This progressive enhancement differs ICC from HCC. HCC shows arterial phase enhancement followed by late phase wash-out. This feature may reflect that fibrosis is late enhancement but retains the contrast agent [14, 25].
4.2.2.4 MRI Findings
On MRI, ICC typically appears hypointense on T1WI and hyperintense on T2WI. T2WI may show central hypointensity due to fibrosis. Dynamic enhanced MRI shows arterial phase peripheral enhancement followed by progressive and centripetal filling in Fig. 4.32. Pooling of contrast agent in the delayed phase indicates fibrosis and may suggest ICC. MRI with cholangiopancreatography (MRCP) is helpful in visualizing the ductal system and in determining the anatomic extent of tumor [14, 25].
4.2.2.5 Other Imaging Findings
Fluorodeoxyglucose positron emission tomography (FDG-PET) can detect mass-forming type of ICC with high sensitivity, but PET is less useful for periductal infiltrating type. When CEUS, CT, or MRI has already been performed, PET-CT for diagnosis of ICC is controversial. The additional utility of PET is also questioned when extrahepatic suspicious lesion on CT or MRI is absent. The benefit of PET for staging ICC is not clear and thus PET should be used selectively [14, 25].
4.2.2.6 Best Imaging Protocol Advices
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Ultrasound, CT, MRI/MRCP, and PET are the most common imaging methods to diagnose and stage ICC.
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Ultrasound is the first imaging method for patients with abdominal pain or obstructive jaundice.
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Almost all ICCs show wash-out in the portal venous/late phase on CEUS, while ICCs often show the late enhancement on contrast enhanced computed tomography (CT) or contrast enhanced magnetic resonance imaging (MRI).
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Intraoperative ultrasound is valuable to guide surgical resection of ICC, to detect intrahepatic biliary stones, and to guide radiofrequency ablation.
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Tumor markers do not have high sensitivity and specificity to make a diagnosis or rule out ICC.
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Biopsy is not necessary if curative resection is planned.
4.2.3 Differential Diagnosis
4.2.3.1 Hepatocellular Carcinoma
The typical enhancement pattern of HCC on CEUS is arterial phase hyperenhancement followed by wash-out in the portal venous and/or late phases under the background of liver cirrhosis. Some studies suggested that there is an overlap between the wash-in pattern of ICC and HCC on CEUS in liver cirrhosis patients or small tumor. The guidelines of the American Association for the study of Liver Diseases (AASLD) have removed CEUS from the diagnostic modalities for HCC, because of the misdiagnosis probability [26].
The arterial enhancement patterns and temporal presentation between ICCs and HCCs are different. Most HCC lesions manifest global hyperenhancement in arterial phase, whereas most ICC lesions manifest peripheral hyperenhancement. Furthermore, recent studies revealed that the timing of wash-out in ICC was much earlier than that in HCC, as well as the degree of wash-out was more marked in ICC than that in HCC. These two differences may differentiate ICC from HCC. Arterial phase hyperenhancement followed by late (>60 s) wash-out of mild degree is now defined to be a refined diagnosis of HCC on CEUS [21, 23, 24, 27].
4.2.4 Pathology
4.2.4.1 General Features
ICC often occurs in a normal liver. Most ICCs are adenocarcinoma with variable differentiation and fibroplasia. A variable and occasionally abundant fibrous stroma is an important characteristic of ICC. Fibrous encapsulation is not seen. ICC directly invades the surrounding hepatic parenchyma and may spread along the portal tracts. The mass-forming type of ICC can be quite large. The periphery of the tumors contains more abundant, proliferating cells, and the center of the tumors is usually more sclerotic and hypocellular (Fig. 4.28). Central necrosis is common, and there may be focal calcification in these areas. Mucin hypersecretion can be visible dilated ducts. Regional lymph nodes are often involved. Lungs are the most common site when blood-borne metastases occur later [15].
4.2.4.2 Staging, Grading, and Classification
Precursor and early lesions
Two types of precursor lesions are considered as precursors or early lesions of ICC arising from the intrahepatic large bile ducts: flat biliary intraepithelial neoplasia (BillN) and papillary intraductal papillary neoplasms (IPN) of the bile duct.
Grading
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Well differentiated
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Moderately differentiated
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Poorly differentiated
TNM classification
Tumor classification depends on the number of lesions, vascular invasion, intrahepatic metastasis, and invasion of adjacent structures.
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T0: No evidence of primary tumor
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T1: Tumor is solitary without vascular invasion
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T2: Multiple tumors (e.g., multifocal disease, satellitosis, and intrahepatic metastasis) with vascular invasion
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T3: Tumor directly invades adjacent structures
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T4: Tumor with any periductal infiltrating component
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N0: No regional lymph nodes metastasis
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N1: Hilar, periduodenal, and peripancreatic lymph nodes metastases
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M0: No distant metastasis
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M1: Distant metastases
4.2.5 Clinical Issues
4.2.5.1 Presentation
ICC is a relatively rare malignancy (accounting for 5–15% of primary liver cancers). Patients with ICC are usually elderly, with a slight male dominance. Etiology of most cases of ICC is not clear, and some cases of ICC are associated with chronic biliary inflammatory, primary sclerosing cholangitis, hepatolithiasis, parasitic biliary infestation, biliary malformations, familial polyposis, congenital hepatic fibrosis, or other risk factors. Clinical symptoms and signs of ICC are often not specific and related to the site of the tumor in the liver, its growth pattern, and the presence of obstruction of biliary tracts. Patients are usually asymptomatic in early stages. ICC without central bile ducts obstruction often attains a large size without being noticed. In advanced stages, patients may present abdominal distension or pain, weight loss, hepatomegaly, or palpable abdominal mass. Biliary tract obstruction is relatively rare, while perihilar cholangiocarcinoma usually presents with cholestasis and cholangitis of the intrahepatic bile ducts [14, 28].
4.2.5.2 Prognosis
ICC is an aggressive cancer with high mortality and poor survival rate as a result of early invasion, widespread metastases, and without effective treatments. The 5-year survival rate after operation is 69% in patients with the intraductal-growth type, and 39% in patients with the mass-forming type. The periductal infiltrating type shows a poor prognosis. The following factors are associated with a poor prognosis: concomitant hepatolithiasis; lymph node spread, macroscopic vascular invasion, positive surgical margins, intrahepatic metastasis, macroscopic vascular invasion, non-curative resection, advanced TNM stage, poor histological differentiation, squamous cell or sarcomatous elements, and mucin phenotype [14, 28].
4.2.5.3 Treatment
Surgical operation is the unique potentially radical treatment. Few patients have chances for surgical resection as many patients present with advanced disease stage. Transarterial chemoembolization (TACE), ablation, and liver transplantation are not recommended for patients with ICC because of uncertain curative effects. Current researches suggest that chemotherapy may be of benefit to patients with margin invasion and/or nodes metastases after surgical resection or with distant metastases [14, 26,27,28].
4.3 Liver Metastases
4.3.1 Terminology
Focal or diffuse malignant involvement of the liver from extrahepatic origins is termed as liver metastases, which are 18–40 times more common than primary liver tumors. The liver is one of the most common organs for metastasis. The complex dual blood supply mode facilitates the inflow of cancer cells from tumors of other organs and distinct microenvironment of sinusoids permits increased trapping of tumor cells, thus supporting the development and invasion of metastases. The early and exact detection of liver metastases is crucial for clinical decision-making and patient management.
4.3.2 Imaging Features
4.3.2.1 Conventional Ultrasound Findings
Conventional ultrasound is the first-line modality for hepatic imaging. It is widely used in routine surveillance, follow-up scanning, and treatment assessment of patients with liver metastases. Most liver metastases occur in normal liver. On US, metastases commonly present as multifocal solid lesions with hypo-, iso-, hyperechoic, or mixed echogenicity depending on the tissue components of the primary tumor and on the presence of necrosis or calcification. Grossly, small lesions may be well-defined hypoechoic nodules; whereas, large lesions are more likely to have heterogeneous echotexture with barely visible margins (Fig. 4.35). A peripheral hypoechoic halo is the most frequent B mode ultrasound feature of liver metastasis, which can also appear as a target sign or “bull’s eye” sign owing to alternating layers of hyperechoic and hypoechoic tissue (Fig. 4.36). However, such morphologic changes are non-specific, especially in lesions smaller than 1 cm [29].
4.3.2.2 Contrast Enhanced Ultrasound Findings
With the aid of microbubbles, contrast ratio between liver parenchyma and focal lesions is greatly increased at contrast enhanced ultrasound (CEUS), which can not only stand out the occult lesions on conventional US, but also be more sensitive for small metastases. Moreover, CEUS is a real-time procedure with better temporal and spatial resolution than contrast-enhanced CT or MRI. It is better able to detect hemodynamic changes and detailed vascular information of hepatic tumors regardless of their rate of perfusion, rendering it an excellent tool for detection and characterization of liver metastases [30].
Typical appearances of liver metastases on CEUS are as follows:
Arterial phase features
Almost all liver metastases show obvious arterial phase enhancement. Generally, there are two enhancement patterns: diffuse homogeneous or heterogeneous hyperenhancement and peripheral rim-like hyperenhancement (Figs. 4.37, 4.38, 4.39, 4.40, 4.41, and 4.42). These patterns are not associated with the degree of tumor vascularity but may be related to nodule size: diffuse hypervascularity is seen more commonly in the lesions smaller than 2 cm, while hepatic metastases larger than 5 cm are more likely to show pronounced rim-like peripheral hyperenhancement [31]. The period for arterial phase enhancement of hepatic metastases is transient and lasts only about 20 s in most cases, which can only be depicted on CEUS and would not be appreciated on CT or MRI imaging. Although such arterial hypervascularity is not specific and provides limited clues for characterization purpose, it may be useful in the treatment response assessment of metastases after local ablation or chemotherapy (Fig. 4.43).
Portal venous and late phase CEUS features
Liver metastases typically present hypoenhancement during the portal venous and late phases after microbubble injection. Rapid and complete wash-out is an invariable evident feature of hepatic metastasis on CEUS. The mass starts to wash-out within 60 s after contrast injection in most cases, and the wash-out onset of some small lesions (≤2 cm) even begins in the late arterial phase (about 20 s after injection). The optimal temporal window to detect metastatic lesions is the early portal venous phase, namely from 40 to 60 s after contrast injection, which provides the best lesion conspicuity. Although hypervascular metastases including neuroendocrine tumors, melanomas, sarcomas, and renal, breast and thyroid neoplasms frequently show a significantly longer wash-out time than hypovascular metastases, virtually almost all metastases unequivocally show complete wash-out after 60 s and remain constant hypoenhancement in the late phase. Marked wash-out is defined as punched out perfusion defects or black hole within 2 min after contrast injection. It should be noted that if a hypoenhanced nodule is relative to the liver but still shows persistent enhancement and eventually becomes marked wash-out after 2 min, the degree of wash-out is still characterized as mild [32, 33].
In HCCs and liver metastases with hypervascularity and a normal liver background on CEUS imaging, various CEUS perfusion parameters on time intensity curve (TIC) may objectively reflect the difference of enhancement patterns between HCCs and liver metastases.
4.3.2.3 CT Findings
Liver metastases present variable enhancement characteristics on contrast enhanced CT. They are best imaged in the portal venous phase and typically appear as a hypoattenuating lesion. Some hypervascular metastases may show marked arterial phase hyperenhancement. However, the benefit of arterial phase observation is controversial for evaluation of such lesions [34].
4.3.2.4 MRI Findings
On non-enhanced MRI, metastases are typically hypointense on T1-weighted images and show various degrees of hyperintensity on T2-weighted images. They show restricted diffusion on DWI and a significant overlap exists for ADC values between metastases and other malignant and benign lesions. Postcontrast MRI imaging features are similar to those of CT. With the use of hepatobiliary-specific contrast agent, metastases show hypointense in hepatobiliary phase, which provides excellent contrast between the liver parenchyma and metastatic lesions and offers superior characterization and greater specificity for diagnosis of liver metastases [34].
4.3.3 Differential Diagnosis
4.3.3.1 Hepatocellular Carcinoma
Most HCCs may occur in cirrhotic liver. Certain atypical HCC, especially poor differentiated HCC tends to show earlier wash-out. However, HCCs frequently show diffuse arterial phase hyperenhancement without a peripheral rim-like, and their degree of wash-out tends to be mild showing substantial enhancement within 2 min after microbubble injection.
4.3.3.2 Intrahepatic Cholangiocarcinoma
ICC usually presents arterial rim-like hyperenhancement and rapid and complete wash-out, posing a challenge for differential diagnosis. On conventional ultrasound, it frequently shows undefined margin with dilated bile ducts in the peripheral region. On CEUS, it may show patchy hypoechoic areas in the peak enhancement and its size may be significantly increased compared with that of baseline appearance.
4.3.3.3 Focal Fatty Liver Change
Focal fatty sparing can mimic a mass and pose a diagnostic dilemma on conventional US and should be differentiated with metastatic lesions in a steatosis liver background. On CEUS, the lesion typically shows synchronization enhancement and wash-out, which can be considered diagnostic.
4.4 Dysplasia Nodules
4.4.1 Terminology
4.4.1.1 Definitions
Most hepatocellular carcinomas (HCCs) occur in patients with cirrhosis caused by various long-term liver damages including chronic hepatitis B or C virus infections, alcoholic liver disease, nonalcoholic steatohepatitis, or autoimmune hepatitis. Long-term chronic inflammations lead to genetic variations leading to hepatocarcinogenesis. Recent data revealed a sequence of changes in hepatic nodules before the occurrence of HCC. Some lesions are considered as precursors of HCCs. In 1995, the International Working Party (IWP) of the World Congresses of Gastroenterology proposed a consensus nomenclature and diagnostic criteria for hepatocellular nodular lesions in cirrhosis, which have been widely adopted. The IWP classified hepatocellular nodular lesions into large regenerative nodule (RN), low-grade dysplastic nodule (LGDN), high-grade dysplastic nodule (HGDN), and HCC. In the latest World Health Organization (WHO) classification of tumors of liver, premalignant lesions of HCCs are classified into large cell change (formerly “dysplasia”), small cell change (formerly “dysplasia”), LGDN, and HGDN. In 2007, the International Consensus Group for Hepatocellular Neoplasia (ICGHN) updated the international consensus on the histopathologic diagnosis of hepatocellular nodular lesions. Stromal invasion, tumor cell invasion into the portal tracts or fibrous septa within vaguely nodular lesions, was defined as a criterion for differential diagnosis of well-differentiated HCC from HGDN [35].
4.4.2 Imaging
The critical alterations during hepatocarcinogenesis detected by imaging methods are simultaneous and gradual decrease of portal tracts, hepatocyte function, Kupffer cell density, and organic anionic transporting polypeptide (OATP) expression, meanwhile development of sinusoidal capillarization and unpaired arterioles. Furthermore, venous drainage changes from hepatic veins to portal veins. In early hepatocarcinogenesis, fat content in the lesions sometimes increases, then in progressed HCC, fat content usually regresses [36].
Nourishing vessel changes in hepatocarcinogenesis in liver cirrhosis are summarized in Fig. 4.44. Three stages according to the pathological and imaging features could be divided during hepatocarcinogenesis in liver cirrhosis: premalignant stage (including DNs, especially HGDNs), early HCC stage (including well-differentiated HCCs of the vague margin and HCCs with diameter ≤2 cm), and progressed HCC stage (including moderately- or poorly-differentiated HCCs) [37].
4.4.2.1 Conventional Ultrasound Findings
Ultrasound (US) is a screening method for HCC surveillance because of its convenience, availability, safety, and cost-effectiveness. However, it is challenging to identify DNs in the background of cirrhotic liver because of their vague margin and small size. On B-mode US, DNs could be hypo-, iso-, or hyperechoic, which is overlapped with RNs or small HCCs. When fat content occurs in the lesions, DNs usually manifest hyperechoic.
4.4.2.2 Contrast Enhanced Ultrasound Findings
Based on angiogenesis and hemodynamic changes during hepatocarcinogenesis, several imaging methods could be used to diagnose cirrhosis-associated nodules. After CT and MRI, contrast enhanced ultrasound (CEUS) has been used as a second-line imaging method to detect and recognize various liver lesions.
Blood supply of DNs maybe both from portal vessels and from newly formed unpaired arteries. Portal tracts usually still remain in DNs, but maybe few and scarred. Unpaired arteries increase in both number and size through HGDN and well-differentiated HCC developmental stages. Therefore, DNs typically show isoenhancement (Fig. 4.45) or hypoenhancement in arterial phase and isoenhancement on subsequent phases on CEUS, as compared to the surrounding liver. In transient phases before the development of unpaired arteries, some DNs could manifest hypoenhancement in arterial phase (Figs. 4.46 and 4.47), because normal arterial and portal supplies reduce simultaneously [38,39,40].
Three types of ultrasound contrast agents (UCAs) are commonly used in liver today, including SonoVue (sulfur hexafluoride with a phospholipid shell), Definity/Luminity (octafluoropropane with a lipid shell), and Sonazoid (perflubutane with a phospholipid shell: hydrogenated egg phosphatidyl serine). The first two UCAs are similar in CEUS imaging manifestation. Both of them belong to vascular pool agents with slow removal over about 5 min after intravenous injection. Sonazoid is a liver-specific contrast agent that is phagocytosed by Kupffer cells on the late phase (termed “postvascular phase” or “Kupffer phase”), and can persist for several hours in the liver and spleen. By contrast, malignant liver lesions usually have few or no Kupffer cells and manifest contrast defect. The Kupffer defect is commonly assessed in the Kupffer phase beginning 6–10 min after intravenous injection and lasting for an hour or more. No DNs showed hypoenhancement in the Kupffer phase [41].
4.4.2.3 CT Findings
Most DNs show non-hypervascularity on arterial phase and isoattenuation on portal venous phase because of the relatively decreased normal arterial and portal venous flow. However, some HGDNs may show hyperenhancement owing to increased neoangiogenesis suggesting the potential probability of HCC transformation [36].
4.4.2.4 MRI Findings
Since MRI can provide better soft-tissue contrast and information of tissue components, current studies suggest MRI is more useful than other imaging methods to identify DNs and HCCs in the background of liver cirrhosis. Pathological changes during hepatocarcinogenesis, including increased cellular density, increased neoangiogenesis followed by decreased portal tracts and decreased OATP expression, could be reflected by various MRI techniques. Compared to CT or MRI with extracellular contrast agents, Gd-EOB-DTPA-enhanced MRI (using a kind of liver-specific contrast agent) can improve the diagnostic accuracy of DNs and HCCs and the sensitivity for the detection of HCCs, especially small HCCs. Before neoangiogenesis in an overt HCC, imaging feature of non-enhancement followed by hypointense on hepatobiliary phase is a strong predictor of precancerous lesion or malignancy (Fig. 4.45) [41].
4.4.2.5 Best Imaging Protocol Advices
-
US every 6 months is universally recommended for patients with high risk of HCC. When DNs are present, they are usually small and difficult to be distinguished from RNs by US.
-
On CEUS, DNs typically show iso-or hypoenhancement on the arterial phase and isoenhancement on subsequent phases.
-
On Gd-EOB-DTPA-enhanced MRI, non-enhancement followed by hypointense on hepatobiliary phase is a strong predictor of precancerous lesion or malignancy (HGDN or early HCC).
4.4.3 Differential Diagnosis
Regerative nodulars
Regerative nodulars (RNs) are usually smaller than 10 mm and innumerable in liver cirrhosis. On CEUS, RNs usually show isoenhancement in the arterial phase. Nearly all RNs show isoenhancement in the portal venous and late phases.
Well-differentiated hepatocellular carcinoma
About 40% of well-differentiated hepatocellular carcinoma (HCCs) demonstrated non-hyperenhancement in the arterial phase on CEUS. Some well-differentiated HCCs manifesting arterial hypoenhancement are confirmed pathologically to have fewer unpaired arteries. Furthermore, 60–90% of well-differentiated HCCs showed almost complete isoenhancement in the portal vein phase and late phase, even though Sonazoid is used. Therefore, DNs and well-differentiated HCCs maybe both hypoenhancement in the arterial phase and isoenhancement in the late phase or in the Kupffer phase on CEUS [36, 39]. To a certain extent, imaging features between HGDNs and well-differentiated HCCs are overlapping, accurate recognition of HGDNs and well-differentiated HCCs is still challenging, not only on CEUS but also on CT and MRI.
The American Association for the Study of Liver Diseases (AASLD) recommends that biopsy is necessary for hepatic lesions less than 2 cm in liver cirrhosis without typical HCC imaging findings, whereas biopsy is not needed for hepatic lesions with characteristic HCC imaging finding [42]. It should be noted that biopsy diagnosis of hepatic border nodules may be also a challenge, because minute biopsy specimens may be inadequate to detect stromal invasion. Core liver biopsy is more recommended than fine needle aspiration, because the specimen obtained is more sufficient and suitable for assessment. Furthermore, the tissue block obtained can be assessed by immunohistochemical technique.
4.4.4 Pathology
4.4.4.1 General Features
DNs may be single or multiple, and may have distinct or indistinct margins on gross examination. The size of DNs ranges from few millimeters to few centimeters. The diameter of most DNs is <15 mm. Based on varying degrees of atypia, DNs are divided into LGDN and HGDN [35].
Low-grade dysplasia nodular
Low-grade dysplasia nodular (LGDNs) usually have no true capsule, but are still distinct from the surrounding cirrhotic liver parenchyma with fibrous tissue around. LGDNs show moderate cell density increase with a homogeneous pattern, and have no cytologic atypia. Large cell changes could be usually found, but there have no architectural changes. Pseudoglands or markedly thickened trabeculaes are non-existent. Some HGDNs have a few unpaired arteries. Nodule-in-nodule type do not appear in LGDNs. Recognition from LGDNs to large regenerative nodules (RNs) is difficult or impossible. Fortunately, there has no significant clinical application value to make a distinction between LGDNs and large RNs at present.
High-grade dysplasia nodular
High-grade dysplasia nodulars (HGDNs) have a distinct or vague margin in the background of cirrhosis. They also lack true capsules, similar to LGDNs. HGDNs commonly have architectural and/or cytologic atypia, but the atypia is not enough to diagnose as HCCs. Increased cell density with an irregular trabecular pattern is often present, and it is usually more than 2–3 times higher than the liver parenchyma. Small cell change is the most commonly seen in HGDNs. Large cell change sometimes can be seen in HGDNs. Other cytological changes, including clear cell change, focal fatty change, and resistance to iron accumulation distinguish HGDN from LGDN. Most HGDNs have some but not in great number of unpaired arteries. Nodule-in-nodule type is occasionally found in HGDNs. The subnodule is usually well-differentiated HCC with a well-defined margin. The most appropriate term for such lesions is “HCC arising in DN.” Definite distinction between HGDN and well-differentiated HCC may be difficult, especially on needle biopsy material. Stromal invasion is a diagnostic criterion for the distinction of HGDN and well-differentiated HCC, and immunostaining for keratins 7 or 19 may be useful for an accurate diagnosis of stromal invasion.
4.4.5 Clinical Issues
4.4.5.1 Presentation
DNs may be single or multiple in cirrhotic liver, and are occasionally found in non-cirrhotic liver.
4.4.5.2 Prognosis
A previous study reported that 27–70% of DNs would enlarge; and the rate of evolution to malignancy was 40–80% [43, 44].
4.4.5.3 Treatment
It is very important to observe the changes in nodule size and blood supply in DNs during the follow-up period in order to predict the development of HCCs as soon as possible.
Abbreviations
- CDFI:
-
Color Doppler flow imaging
- CEUS:
-
Contrast enhanced ultrasound
- CT:
-
Computed tomography
- DWI:
-
Diffusion-weighted imaging
- HCC:
-
Hepatocellular carcinoma
- HGDN:
-
High-grade dysplastic nodule
- ICC:
-
Intrahepatic cholangiocarcinoma
- ICGHN:
-
International Consensus Group for Hepatocellular Neoplasia
- IWP:
-
International Working Party
- LGDN:
-
Low-grade dysplastic nodule
- MRI:
-
Magnetic resonance image
- OATP:
-
Organic anionic transporting polypeptide
- PET:
-
Positron emission tomography
- RN:
-
Regenerative nodule
- UCA:
-
Ultrasound contrast agent
- US:
-
Ultrasonography
- WHO:
-
World Health Organization
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Dong, Y. et al. (2021). Malignant Liver Tumors. In: Wang, WP., Dong, Y., Dietrich, C.F., Jung, E.M. (eds) Contrast-Enhanced Ultrasound Imaging of Hepatic Neoplasms. Springer, Singapore. https://doi.org/10.1007/978-981-16-1761-4_4
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