Abstract
Except for individuals with variations in the pericentric heterochromatic chromosomal regions (including acrocentric short arms), a distinction of homologue chromosomes on a single-cell level is not possible. Due to this limitation, various questions of scientific and diagnostic relevance could not be studied by now. Based on copy number variations (CNV) spanning up to several megabasepair of DNA, we developed a molecular cytogenetic approach for an interindividual differentiation of homologue chromosomes, the so-called parental origin determination FISH (pod-FISH) technique. For this, all human chromosomes were covered with CNV-spanning BAC-probes in one- up to five-color chromosome-specific pod-FISH sets. With this approach to study the parental origin of individual human chromosomes on a single-cell level, new horizons for diagnostics and basic research were opened.
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Weise, A., Liehr, T. (2017). Parental Origin Determination FISH: Pod-FISH. In: Liehr, T. (eds) Fluorescence In Situ Hybridization (FISH). Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-52959-1_32
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DOI: https://doi.org/10.1007/978-3-662-52959-1_32
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Publisher Name: Springer, Berlin, Heidelberg
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Online ISBN: 978-3-662-52959-1
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