Skip to main content
SpringerLink
Log in

Molekulare Diagnostik bei Allergie gegen Säugetiere

  • Chapter
  • First Online:
Molekulare Allergiediagnostik

  • 1705 Accesses

Zusammenfassung

Aus allergologischer Sicht sind Säugetiere reiche Allergenquellen, deren Proteine sowohl im häuslichen als auch im beruflichen Umfeld häufig zu allergischen Symptomen führen. In Europa und den USA sind Haustiere sehr beliebt: Je nach Region haben 30–60 % aller Haushalte ein Haustier. Die häufigsten Tiere sind Hunde und Katzen, gefolgt von Fischen, kleinen Säugetieren wie z. B. Kaninchen, Meerschweinchen und Hamster, sowie Vögel. Viele der in Tierhaaren vorkommenden Allergene sind mittlerweile gut charakterisiert. Neben den beiden Hauptfamilien, den Lipokalinen und den Serumalbuminen, gibt es einzelne Allergene, die zu anderen Proteinfamilien gehören, wie z. B. Sekretoglobine, Cystatine, Kallikreine oder Latherine. Es ist durchaus wahrscheinlich, dass in Zukunft noch weitere Vertreter dieser Proteinfamilien identifiziert werden.

Die in Hauttests und in der In-vitro-IgE-Diagnose verwendeten Tierhaar- und Epithelienextrakte haben den Nachteil, dass sie nur schwer standardisierbar sind und kreuzreaktive Moleküle enthalten. Dabei handelt es sich hauptsächlich um Serumalbumine, jedoch wurden neuerdings auch einige kreuzreaktive Lipokaline identifiziert. Die Diagnose mittels Einzelallergenen bietet potenziell den Vorteil, die allergieauslösende Tierspezies gezielt zu bestimmen, um dem Patienten eine bessere Beratung sowie die für ihn geeignete Immuntherapie anbieten zu können.

Der Beitrag basiert auf einer Publikation der Autoren, die 2011 im Allergo Journal erschienen ist (Hilger C, Kleine-Tebbe J. Inhalative Säugetierallergene: Lipokaline und Serumalbumine. Allergo J 2011; 20: 142–144) und nun als Buchkapitel aktualisiert und erweitert wurde.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 49.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 59.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

Literatur

  • Adedoyin J, Gronlund H, Oman H, Johansson SGO, van Hage M (2007) Cat IgA, representative of new carbohydrate cross-reactive allergens. J Allergy Clin Immunol 119:640–645

    Article  CAS  PubMed  Google Scholar 

  • Baker J, Berry A, Boscato LM, Gordon S, Walsh BJ, Stuart MC (2001) Identification of some rabbit allergens as lipocalins. Clin Exp Allergy 31:303–312

    Article  CAS  PubMed  Google Scholar 

  • Bulone V, Krogstad-Johnsen T, Smestad-Paulsen B (1998) Separation of horse dander allergen proteins by two-dimensional electrophoresis--molecular characterisation and identification of Equ c 2.0101 and Equ c 2.0102 as lipocalin proteins. Eur J Biochem 253:202–211

    Article  CAS  PubMed  Google Scholar 

  • Cabañas R, López-Serrano MC, Carreira J, Ventas P, Polo F, Caballero MT, Contreras J, Barranco P, Moreno-Ancillo A (2000) Importance of albumin in cross-reactivity among cat, dog and horse allergens. J Investig Allergol Clin Immunol 10:71–77

    PubMed  Google Scholar 

  • Cavaggioni A, Mucignat-Caretta C (2000) Major urinary proteins, [alpha]2U-globulins and aphrodisin. Biochim Biophys Acta 1482:218–228

    Article  CAS  PubMed  Google Scholar 

  • Chruszcz M, Mikolajczak K, Mank N, Majorek KA, Porebski PJ, Minor W (2013) Serum albumins-unusual allergens. Biochim Biophys Acta 1830:5375–5381

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Commins SP, James HR, Kelly LA, Pochan SL, Workman LJ, Perzanowski MS, Kocan KM, Fahy JV, Nganga LW, Ronmark E, Cooper PJ, Platts-Mills TAE (2011) The relevance of tick bites to the production of IgE antibodies to the mammalian oligosaccharide galactose-α-1,3-galactose. J Allergy Clin Immunol 127:1286–1293.e6

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Dandeu JP, Rabillon J, Divanovic A, Carmi-Leroy A, David B (1993) Hydrophobic interaction chromatography for isolation and purification of Equ.cl, the horse major allergen. J Chromatogr 621:23–31

    Article  CAS  PubMed  Google Scholar 

  • Fahlbusch B, Rudeschko O, Szilagyi U, Schlott B, Henzgen M, Schlenvoigt G, Schubert H (2002) Purification and partial characterization of the major allergen, Cav p 1, from guinea pig Cavia porcellus. Allergy 57:417–422

    Article  CAS  PubMed  Google Scholar 

  • Flower DR, North ACT, Sansom CE (2000) The lipocalin protein family: structural and sequence overview. Biochim Biophys Acta 1482:9–24

    Article  CAS  PubMed  Google Scholar 

  • Gordon S, Tee RD, Stuart MC, Newman TAJ (2001) Analysis of allergens in rat fur and saliva. Allergy 56:563–567

    Article  CAS  PubMed  Google Scholar 

  • Goubran Botros H, Poncet P, Rabillon J, Fontaine T, Laval JM, David B (2001) Biochemical characterization and surfactant properties of horse allergens. Eur J Biochem 268:3126–3136

    Article  CAS  PubMed  Google Scholar 

  • Gregoire C, Rosinski-Chupin I, Rabillon J, Alzari PM, David B, Dandeu JP (1996) cDNA cloning and sequencing reveal the major horse allergen Equ c1 to be a glycoprotein member of the lipocalin superfamily. J Biol Chem 271:32951–32959

    Article  CAS  PubMed  Google Scholar 

  • Gronlund H, Adedoyin J, Commins SP, Platts-Mills TA, van Hage M (2009) The carbohydrate galactose-alpha-1,3-galactose is a major IgE-binding epitope on cat IgA. J Allergy Clin Immunol 123:1189–1191

    Article  PubMed Central  PubMed  Google Scholar 

  • Grzyb J, Latowski D, Strzalka K (2006) Lipocalins – a family portrait. J Plant Physiol 163:895–915

    Article  CAS  PubMed  Google Scholar 

  • Haftenberger M, Laußmann D, Ellert U, Kalcklösch M, Langen U, Schlaud M, Schmitz R, Thamm M (2013) Prävalenz von Sensibilisierungen gegen Inhalations- und Nahrungsmittelallergene (DEGS1). Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz 56:687–697

    Article  CAS  PubMed  Google Scholar 

  • van Hage M, Pauli G (2014) New vaccines for Mammalian allergy using molecular approaches. Front Immunol 5:81

    PubMed Central  PubMed  Google Scholar 

  • Heinzerling LM, Burbach GJ, Edenharter G, Bachert C, Bindslev-Jensen C, Bonini S, Bousquet J, Bousquet-Rouanet, Bousquet PJ, Bresciani M, Bruno A, Burney P, Canonica GW, Darsow U, Demoly P, Durham, Fokkens WJ, Giavi S, Gjomarkaj M, Gramiccioni C, Haahtela T, Kowalski ML, Magyar P, Muraközi G, Orosz M, Papadopoulos NG, Röhnelt C, Stingl G, Todo-Bom A, von Mutius E, Wiesner A, Wöhrl S, Zuberbier TI (2009) GA 2 LEN skin test study I: GA²LEN harmonization of skin prick testing: novel sensitization patterns for inhalant allergens in Europe. Allergy 64:1498–1506

    Article  CAS  PubMed  Google Scholar 

  • Hentges F, Léonard C, Arumugam K, Hilger C (2014) Immune responses to inhalant Mammalian allergens. Front Immunol 5:234

    Article  PubMed Central  PubMed  Google Scholar 

  • Heutelbeck AR, Janicke N, Hilgers R, Kütting B, Drexler H, Hallier E, Bickeböller H (2007) German cattle allergy study (CAS): public health relevance of cattle-allergic farmers. Int Arch Occup Environ Health 81:201–208

    Article  PubMed  Google Scholar 

  • Hilger C, Kohnen M, Grigioni F, Lehners C, Hentges F (1997) Allergic cross-reactions between cat and pig serum albumin. Study at the protein and DNA levels. Allergy 52:179–187

    Article  CAS  PubMed  Google Scholar 

  • Hilger C, Swiontek K, Hentges F, Donnay C, de Blay F, Pauli G (2010) Occupational inhalant allergy to pork followed by food allergy to pork and chicken: sensitization to hemoglobin and serum albumin. Int Arch Allergy Immunol 151:173–178

    Article  CAS  PubMed  Google Scholar 

  • Hilger C, Swiontek K, Kler S, Diederich C, Lehners C, Vogel L, Vieths S, Hentges F (2011) Evaluation of two new recombinant guinea-pig lipocalins, Cav p 2 and Cav p 3, in the diagnosis of guinea-pig allergy. Clin Exp Allergy 41:899–908

    Article  CAS  PubMed  Google Scholar 

  • Hilger C, Kuehn A, Hentges F (2012) Animal lipocalin allergens. Curr Allergy Asthma Rep 12:438–447

    Article  CAS  PubMed  Google Scholar 

  • Hilger C, Swiontek K, Arumugam K, Lehners C, Hentges F (2012) Identification of a new major dog allergen highly cross-reactive with Fel d 4 in a population of cat- and dog-sensitized patients. J Allergy Clin Immunol 129:1149–1151 (e2)

    Article  CAS  PubMed  Google Scholar 

  • Hilger C, Kler S, Arumugam K, Revets D, Muller CP, Charpentier C, Lehners C, Morisset M, Hentges F (2014) Identification and isolation of a Fel d 1-like molecule as a major rabbit allergen. J Allergy Clin Immunol 133:759–766

    Article  CAS  PubMed  Google Scholar 

  • Hilger C, Kler S, Hentges F (2014) Reply: To PMID 24369805. J Allergy Clin Immunol 133:284–285

    Article  PubMed  Google Scholar 

  • Hilger C, Dubey VP, Lentz D, Davril C, Revets D, Muller CP, Diederic C, De La Barrière H, Codreanu-More F, Morisset M, Lehners C, De PK, Hentges F (2015) Male-specific submaxillary gland protein, a lipocalin allergen of Golden hamster, differs from lipocalin allergens of Siberian and Roborovski dwarf hamsters. Int Arch Allergy Immunol 166:30–40

    Article  CAS  PubMed  Google Scholar 

  • Ichikawa K, Vailes LD, Pomes A, Chapman MD (2001) Molecular cloning, expression and modelling of cat allergen, cystatin (Fel d 3), a cysteine protease inhibitor. Clin Exp Allergy 31:1279–1286

    Article  CAS  PubMed  Google Scholar 

  • Jakob T, Hilger C, Hentges F (2013) Clinical relevance of sensitization to cross-reactive lipocalin Can f 6. Allergy 68:690–691

    Article  CAS  PubMed  Google Scholar 

  • Jeal H, Jones M (2010) Allergy to rodents: an update. Clin Exp Allergy 40:1593–1601

    Article  CAS  PubMed  Google Scholar 

  • Jeal H, Harris J, Draper A, Taylor AN, Cullinan P, Jones M (2009) Dual sensitization to rat and mouse urinary allergens reflects cross-reactive molecules rather than atopy. Allergy 64:855–861

    Article  CAS  PubMed  Google Scholar 

  • Konieczny A, Morgenstern JP, Bizinkauskas CB, Lilley CH, Brauer AW, Bond JF, Aalberse RC, Wallner BP, Kasaian MT (1997) The major dog allergens, Can f 1 and Can f 2, are salivary lipocalin proteins: cloning and immunological characterization of the recombinant forms. Immunology 92:577–586

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Konradsen JR, Fujisawa T, van Hage M, Hedlin G, Hilger C, Kleine-Tebbe J, Matsui EC, Roberts G, Rönmark E, Platts-Mills T (2015) Allergy to furry animals: New insights, diagnostic approaches, and challenges. J Allergy Clin Immunol 135:616–625

    Article  PubMed  Google Scholar 

  • Mattsson L, Lundgren T, Everberg H, Larsson H, Lidholm J (2009) Prostatic kallikrein: a new major dog allergen. J Allergy Clin Immunol 123:362–368

    Article  CAS  PubMed  Google Scholar 

  • Mattsson L, Lundgren T, Olsson P, Sundberg M, Lidholm J (2010) Molecular and immunological characterization of Can f 4: a dog dander allergen cross-reactive with a 23 kDa odorant-binding protein in cow dander. Clin Exp Allergy 40:1276–1287

    Article  CAS  PubMed  Google Scholar 

  • Morgenstern JP, Griffith IJ, Brauer AW, Rogers BL, Bond JF, Chapman MD, Kuo MC (1991) Amino acid sequence of Fel dI, the major allergen of the domestic cat: protein sequence analysis and cDNA cloning. Proc Natl Acad Sci USA 88:9690–9694

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Niespodziana K, Focke-Tejkl M, Linhart B, Civaj V, Blatt K, Valent P, van Hage M, Grönlund H, Valenta R (2011) A hypoallergenic cat vaccine based on Fel d 1-derived peptides fused to hepatitis B PreS. J Allergy Clin Immunol 127:1562–1570.e6

    Article  CAS  PubMed  Google Scholar 

  • Nilsson OB, Binnmyr J, Zoltowska A, Saarne T, van Hage M, Grönlund H (2012) Characterization of the dog lipocalin allergen Can f 6: the role in cross-reactivity with cat and horse. Allergy 67:751–757

    Article  CAS  PubMed  Google Scholar 

  • Patel D, Couroux P, Hickey P, Salapatek AM, Laidler P, Larché M, Hafner RP (2013) Fel d 1-derived peptide antigen desensitization shows a persistent treatment effect 1 year after the start of dosing: a randomized, placebo-controlled study. J Allergy Clin Immunol 131:103–109.e1–7

    Article  CAS  PubMed  Google Scholar 

  • Price JA, Longbottom JL (1988) Allergy to rabbits. II. Identification and characterization of a major rabbit allergen. Allergy 43:39–48

    Article  CAS  PubMed  Google Scholar 

  • Rautiainen J, Rytkönen M, Parkkinen S, Pentikäinen J, Linnala-Kankkunen A, Virtanen T, Pelkonen J, Mäntyjärvi R (1995) cDNA cloning and protein analysis of a bovine dermal allergen with homology to psoriasin. J Invest Dermatol 105:660–663

    Article  CAS  PubMed  Google Scholar 

  • Saarelainen S, Rytkonen-Nissinen M, Rouvinen J, Taivainen A, Auriola S, Kauppinen A, Kinnunen T, Virtanen T (2008) Animal-derived lipocalin allergens exhibit immunoglobulin E cross-reactivity. Clin Exp Allergy 38:374–381

    Article  CAS  PubMed  Google Scholar 

  • Schmitz R, Ellert U, Kalcklösch M, Dahm S, Thamm M (2013) Patterns of Sensitization to Inhalant and Food Allergens – Findings from the German Health Interview and Examination Survey for Children and Adolescents. Int Arch Allergy Immunol 162:263–270

    Article  CAS  PubMed  Google Scholar 

  • Senti G, Crameri R, Kuster D, Johansen P, Martinez-Gomez JM, Graf N, Steiner M, Hothorn LA, Grönlund H, Tivig C, Zaleska A, Soyer O, van Hage M, Akdis CA, Akdis M, Rose H, Kündig TM (2012) Intralymphatic immunotherapy for cat allergy induces tolerance after only 3 injections. J Allergy Clin Immunol 129:1290–1296

    Article  CAS  PubMed  Google Scholar 

  • Smith W, Butler AJ, Hazell LA, Chapman MD, Pomés A, Nickels DG, Thomas WR (2004) Fel d 4, a cat lipocalin allergen. Clin Exp Allergy 34:1732–1738

    Article  CAS  PubMed  Google Scholar 

  • Smith W, O’Neil SE, Hales BJ, Chai TLY, Hazell LA, Tanyaratsrisakul S, Piboonpocanum S, Thomas WR (2011) Two Newly Identified Cat Allergens: The von Ebner Gland Protein Fel d 7 and the Latherin-Like Protein Fel d 8. Int Arch Allergy Immunol 156:159–170

    Article  CAS  PubMed  Google Scholar 

  • Spitzauer S, Schweiger C, Sperr WR, Pandjaitan B, Valent P, Mühl S, Ebner C, Scheiner O, Kraft D, Rumpold H (1994) Molecular characterization of dog albumin as a cross-reactive allergen. J Allergy Clin Immunol 93:614–627

    Article  CAS  PubMed  Google Scholar 

  • Torres JA, de Las Heras M, Maroto AS, Vivanco F, Sastre J, Pastor-Vargas C (2014) Molecular and immunological characterization of the first allergenic lipocalin in hamster: the major allergen from Siberian hamster (Phodopus sungorus). J Biol Chem 289:23382–23388

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Ylönen J, Mäntyjärvi R, Taivainen A, Virtanen T (1992) IgG and IgE antibody responses to cow dander and urine in farmers with cow-induced asthma. Clin Exp Allergy 22:83–90

    Article  PubMed  Google Scholar 

  • Zahradnik E, Raulf M (2014) Animal allergens and their presence in the environment. Front Immunol 5:76

    Article  PubMed Central  PubMed  Google Scholar 

  • Zahradnik E, Sander I, Bruckmaier L, Flagge A, Fleischer C, Schierl R, Nowak D, Sültz J, Spickenheuer A, Noss I, Brüning T, Raulf-Heimsoth M (2011) Development of a sandwich ELISA to measure exposure to occupational cow hair allergens. Int Arch Allergy Immunol 155:225–233

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Allergie- und Asthma-Zentrum Westend, Praxis Hanf, Ackermann und Kleine-Tebbe, Berlin, Deutschland

    PD Dr. Jörg Kleine-Tebbe

  2. Department of Infection & Immunity, Luxembourg Institute of Health, Rue Henri Koch 29, 4354, Esch-sur-Alzette, Luxemburg

    Dr. rer. nat. Christiane Hilger

Authors
  1. Dr. rer. nat. Christiane Hilger
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. PD Dr. Jörg Kleine-Tebbe
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Allergie- und Asthma-Zentrum Westend, Praxis Hanf, Ackermann und Kleine-Tebbe, Berlin, Germany

    Jörg Kleine-Tebbe

  2. Universitätsklinikum Gießen und Marburg, Klinik für Dermatologie und Allergologie, Freiburg im Breisgau, Germany

    Thilo Jakob

Rights and permissions

Reprints and permissions

Copyright information

© 2015 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Hilger, C., Kleine-Tebbe, J. (2015). Molekulare Diagnostik bei Allergie gegen Säugetiere. In: Kleine-Tebbe, J., Jakob, T. (eds) Molekulare Allergiediagnostik. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-45221-9_17

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-45221-9_17

  • Published:

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-45220-2

  • Online ISBN: 978-3-662-45221-9

  • eBook Packages: Medicine (German Language)

Publish with us

Policies and ethics

Search

Navigation