Abstract
Electron transfer from the reduced tyrosine YD and cytochrome b559 (Cyt b559) to the S2 and S3 states of photosystem II was investigated at the temperature of 195 K. Electron transfer reactions were followed by measuring EPR signals of tyrosine YD ·, oxidized Cyt b559 and the S2-state multiline signal. Long term incubation (∼90 days) at 195 K causes decay of the majority of S2 centers up to ∼40% of initial value, while in this time scale the intensity of YD· radical increases less than 10%. Samples advanced to S3 state demonstrates an increasing behavior of the S2-state multiline signal intensity in the beginning of incubation (∼20 days) and slow decay up to 40% of maximal amplitude during further incubation of the samples. Similarly to the S2 sample, small increase in YD· radical signal was observed during the S3 decay. However, in both types of samples prepared in S2 and S3 states after 90 days of incubation the signal of oxidized Cyt b559 is increased from 45%–50% up to 100% maximal intensity. The results obtained in this study support the conclusion of our early investigations which claimed the reduced Cyt b559 as electron source for the S2 and S3 states.
Access provided by Autonomous University of Puebla. Download to read the full chapter text
Chapter PDF
Similar content being viewed by others
References
Barber J (2003) Photosystem II: The Engine of Life. Quart. Rev. Biophys. 36: 71–89
Berthold DA, Babcock GT, Yocum CF (1981) A Highly Resolved, Oxygen-Evolving Photosystem II Preparation from Spinach Thylakoid Membranes. FEBS Lett. 134: 231–234
Buser CA, Thompson LK, Diner BA, Brudvig GW (1990) Electron-Transfer Reactions in Manganese-Depleted Photosystem II. Biochemistry 29: 8977–8985
Debus RJ (1992) The Manganese and Calcium Ions of Photosynthetic Oxygen Evolution. Biochim. Biophys. Acta 1102: 269–352
Diner BA, Babcock GT (1996) Structure, Dynamics, and Energy Conversion Efficiency in Photosystem II. In: Ort DR, Yocum CF (eds.), Oxygenic Photosynthesis: The Light Reactions. Kluwer Acad. Publ.: Dordrecht, pp. 213–247
Faller P, Maly T, Rutherford AW, MacMillan F (2001) Chlorophyll and Carotenoid Radicals in Photosystem II Studied by Pulsed ENDOR. Biochemistry 40: 320–326
Feyziyev Y, Van Rotterdam BJ, Bernat G, Styring S (2003) Electron Transfer from Cytochrome b559 and TyrosineD to the S2 and S3 States of the Water Oxidizing Complex in Photosystem II. Chemical Physics 294: 415–431
Hanley J, Deligiannakis Y, Pascal A, Faller P, Rutherford AW (1999) Carotenoid Oxidation in Photosystem II. Biochemistry 38: 8189–8195
Stewart DH, Brudvig GW (1998) Cytochrome b559 of Photosystem II. Biochim. Biophys. Acta 1367: 63–87
Styring S, Rutherford AW (1987) In the Oxygen-Evolving Complex of Photosystem II the S0 State Is Oxidized to the S1 State by YD + (Signal IIslow). Biochemistry 26: 2401–2405
Styring S, Rutherford AW (1988) Deactivation Kinetics and Temperature Dependence of the S-State Transitions in the Oxygen-Evolving System of Photosystem II Measured by EPR Spectroscopy. Biochim. Biophys. Acta 933: 378–387
Thompson L, Brudvig G (1988) Cytochrome-b559 May Function to Protect Photosystem II from Photoinhibition. Biochemistry 27: 6653–6658
Vass I, Styring S (1991) pH Dependent Charge Equilibria between Tyrosine-D and the S-States in Photosystem II. Estimation of Relative Midpoint Redox Potentials. Biochemistry 30: 830–839
Völker M, Ono T, Inoue Y, Renger G (1985) Effect of Trypsin on the PSII Particles. Correlation between Hill Activity, Mn-Abundance and Peptide Pattern. Biochim. Biophys. Acta 806: 25–34
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
Copyright information
© 2013 Zhejiang University Press, Hangzhou and Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Feyziyev, Y., Styring, S. (2013). Charge Equilibrium Reactions S2 and S3 States of Photosystem II with Cyt b559 and Tyrosine YD . In: Photosynthesis Research for Food, Fuel and the Future. Advanced Topics in Science and Technology in China. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-32034-7_45
Download citation
DOI: https://doi.org/10.1007/978-3-642-32034-7_45
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-32033-0
Online ISBN: 978-3-642-32034-7
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)