Abstract
Two-component signal transduction is the primary signaling mechanism used for global regulation of cell response to changes in the environment. DNA microarray analysis identified genes up-regulated by acid stress in cyanobacteria Synechocystis sp. PCC 6803. Several of these altered genes are thought to be response regulators that are directly involved in this type of stress. Deletion mutants of response regulator genes were constructed and survivability was compared between the cells transfected with mutant and wild-type genes in a low-pH medium. Among these, deletion of slr0081 affected the growth rate under conditions of acid stress (pH 6.0). We examined the genome-wide expression of genes in ΔsIr0081 mutant cells by using DNA microarray in an attempt to determine whether slr0081 is involved in the regulation of other acid stress responsive genes. Our findings by quantitative real-time RT PCR revealed that down regulation of acid responsive genes slr0967 and sll0939 occurs by deletion of slr0081.
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© 2013 Zhejiang University Press, Hangzhou and Springer-Verlag Berlin Heidelberg
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Tanaka, Y. et al. (2013). Involvement of slr0081, a Two-Component Signal-Transduction System Response Regulator, in Acid Stress Tolerance in Synechocystis sp. PCC 6803. In: Photosynthesis Research for Food, Fuel and the Future. Advanced Topics in Science and Technology in China. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-32034-7_128
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DOI: https://doi.org/10.1007/978-3-642-32034-7_128
Publisher Name: Springer, Berlin, Heidelberg
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