Abstract
In vitro models for the assessment of antithrombotic activity of an anticoagulant or antiplatelet can be carried out using human blood or blood from various animal species where the in vivo antithrombotic efficacy would be evaluated.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References and Further Reading
In Vitro Models of Thrombosis
Abulencia JP, Tien N, McCarty OJT, Plymire D, Mousa SA, Konstantopoulos K (2001) Comparative antiplatelet efficacy of anovel, nonpeptide GPIIb/IIIa antagonists (XV454) and abciximab (c7E3) in flow models of thrombosis. Arterioscler Thromb Vasc Biol 21:149–156
Alveriadou BR, Moake JL, Turner NA, Ruggeri ZM, Folie BJ, Phillips MD, Schreiber A, Hrinda ME, McEntire LV (1993) Real-time analysis of shear dependent thrombus formation and its blockade by inhibitors of von Willebrand factor binding to platelets. Blood 81:1263–1276
Evangelista V, Manarini S, Rotondo S et al (1996) Platelet/polymorphonuclear leukocyte interaction in dynamic conditions: evidence of adhesion cascade and cross talk between P-selectin and the beta 2 integrin CD11b/CD18. Blood 88:4183–4194
Jen CJ, McIntire LV (1984) Characteristics of shear-induced aggregation in whole blood. J Lab Clin Med 103:115–124
Konstantopoulos K, Kamat SG, Schafer AI et al (1995) Shear-induced platelet aggregation is inhibited by in vivo infusion of an anti-glycoprotein IIb/IIIa antibody fragment, c7E3 Fab, in patients undergoing coronary angioplasty. Circulation 91:1427–1431
Konstantopoulos K, Kukreti S, McIntire LV (1998) Biomechanics of cell interactions in shear fields. Adv Drug Delivery Rev 33:141–164
Mousa SA, Abulencia JP, McCarty OJ et al (2002) Comparative efficacy between glycoprotein IIb/IIIa antagonists roxifiban and orbofiban in inhibiting platelet responses in flow models of thrombosis. J Cardiovasc Pharmacol 39:552–560
Sweeney JD, Labuzzetta JW, Michielson CE, Fitzpatrick JE (1989) Whole blood aggregation using impedance and particle counter methods. Am J Clin Pathol 92:794–797
Turitto VT (1982) Blood viscosity, mass transport, and thrombogenesis. Prog Hemost Thromb 6:139–177
Cone-and-Plate Viscometry Under Shear-Flow Cytometry
Konstantopoulos K, Kamat SG, Schafer AI et al (1995) Shear-induced platelet aggregation is inhibited by in vivo infusion of an anti-glycoprotein IIb/IIIa antibody fragment, c7E3 Fab, in patients undergoing coronary angioplasty. Circulation 91:1427–1431
Konstantopoulos K, Kukreti S, McIntire LV (1998) Biomechanics of cell interactions in shear fields. Adv Drug Delivery Rev 33:141–164
Platelet Adhesion and Aggregation Under Dynamic Shear
Folie BJ, McIntire LV, Lasslo A (1988) Effects of a novel antiplatelet agent in mural thrombogenesis on collagen-coated glass. Blood 72:1393–1400
Konstantopoulos K, Kamat SG, Schafer AI et al (1995) Shear-induced platelet aggregation is inhibited by in vivo infusion of an anti-glycoprotein IIb/IIIa antibody fragment, c7E3 Fab, in patients undergoing coronary angioplasty. Circulation 91:1427–1431
Mousa SA, Abulencia JP, McCarty OJ et al (2002) Comparative efficacy between glycoprotein IIb/IIIa antagonists roxifiban and orbofiban in inhibiting platelet responses in flow models of thrombosis. J Cardiovasc Pharmacol 39:552–560
Cell Adhesion to Immobilized Platelets: Parallel-Plate Flow Chamber
Abulencia JP, Tien N, McCarty OJT et al (2001) Comparative antiplatelet efficacy of a novel, nonpeptide GPIIb/IIIa antagonist (XV454) and abciximab (c7E3) in flow models of thrombosis. Arterioscler Thromb Vasc Biol 21:149–156
Mousa SA, Forsythe M, Bozarth J, Youssef A, Wityak J, Olson R, Sielecki T (1998) XV454, a novel nonpeptide small-molecule platelet GIIb/IIIa antagonist with comparable platelet alpha(IIb)beta3-binding kinetics to c7E3. J Cardiovasc Pharmacol 32(5):736–744
Mousa SA, Abulencia JP, McCarty OJ et al (2002) Comparative efficacy between glycoprotein IIb/IIIa antagonists roxifiban and orbofiban in inhibiting platelet responses in flow models of thrombosis. J Cardiovasc Pharmacol 39:552–560
Tsao PW, Bozarth JM, Jackson SA, Forsythe MS, Flint SK, Mousa SA (1995) Platelet GPIIb/IIIa receptor occupancy studies using a novel fluoresceinated cyclic Arg-Gly-Asp peptide. Thromb Res 77(6):543–556
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer-Verlag Berlin Heidelberg
About this entry
Cite this entry
Mousa, S.A. (2015). In Vitro Models of Thrombosis. In: Hock, F. (eds) Drug Discovery and Evaluation: Pharmacological Assays. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27728-3_12-1
Download citation
DOI: https://doi.org/10.1007/978-3-642-27728-3_12-1
Received:
Accepted:
Published:
Publisher Name: Springer, Berlin, Heidelberg
Online ISBN: 978-3-642-27728-3
eBook Packages: Springer Reference Biomedicine and Life SciencesReference Module Biomedical and Life Sciences