Abstract
In an enzymatically responsive system, a suitable enzyme is used as a stimulus for a control release or delivery at a specifically targeted site where that enzyme is designed in such a way that can work at certain controlled conditions (such as temperature, pH). Enzyme-responsive hydrogels prepared from cellulose along with other materials have suitable macromolecular networks and can work in controlled environment. Specifically designed enzymatic stimuli-responsive system, one of the highly explored techniques, popularly explored to add a triggerable agent (such as a polymer or a lipid) that can encapsulate the active component in a protective manner. Usually, this active agent is responsive to degradation or swelling when it reaches at the target site. An enzymatic stimulus-responsive system is highly attractive field of research due to its many potential applications (e.g., in controlled release, drug delivery, and other areas of life and material sciences). This chapter gives a brief overview on the design and uses of enzyme-responsive hydrogels based on cellulose and other polymers for their various applications in different fields including in controlled drug delivery and other areas of biomedical and material sciences.
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Hoffman AS (2004) Applications of “Smart Polymers” as biomaterials, 2nd edn. Elsevier Academic Press, London
Kopecek J (2003) Smart and genetically engineered biomaterials and drug delivery systems. Eur J Pharm Sci 20:1–16
Mano JF (2008) Stimuli-responsive polymeric systems for biomedical applications. Adv Eng Mater 10:515–527
Schmaljohann D (2006) Thermo- and pH-responsive polymers in drug delivery. Adv Drug Deliv Rev 58:1655–1670
Roy D, Cambre JN, Sumerlin BS (2010) Future perspectives and recent advances in stimuli-responsive materials. Prog Polym Sci 35:278–301
Ghadiali JE, Stevens MM (2008) Enzyme-responsive nanoparticle systems. Adv Mater 20:4359–4363
Williams RJ, Mart RJ, Ulijn RV (2010) Exploiting biocatalysis in peptide self-assembly. Biopolymers 94:107–117
Zelzer M, Ulijn RV (2010) Next-generation peptide nanomaterials: molecular networks, interfaces and supramolecular functionality. Chem Soc Rev 39:3351–3357
Ulijn RV (2006) Enzyme-responsive materials: a new class of smart biomaterials. J Mater Chem 16:2217–2225
Ghadiali JE, Cohen BE, Stevens MM (2010) Protein kinase-actuated resonance energy transfer in quantum dot−peptide conjugates. ACS Nano 4:4915–4919
Privman M, Tam TK, Pita M, Katz E (2008) Network analysis of biochemical logic for noise reduction and stability: a system of three coupled enzymatic and gates. J Am Chem Soc 131:1314–1321
Bonomi R, Cazzolaro A, Sansone A, Scrimin P, Prins LJ (2011) Detection of enzyme activity through catalytic signal amplification with functionalized gold nanoparticles. Angew Chem Int Ed 50:2307–2312
Zhao WR, Zhang HT, He QJ, Li YS, Gu JL, Li L, Li H, Shi JL (2011) A glucose-responsive controlled release of insulin system based on enzyme multilayers-coated meso porous silica particles. Chem Commun 47:9459–9461
Gordijo CR, Shuhendler AJ, Wu XY (2010) Glucose-responsive bioinorganic nanohybrid membrane for self-regulated insulin release. Adv Funct Mater 20:1404–1412
Hahn ME, Gianneschi NC (2011) Enzyme-directed assembly and manipulation of organic nanomaterials. Chem Commun 47:11814–11821
Welser K, Adsley R, Moore BM, Chan WC, Aylott JW (2011) Protease sensing with nanoparticle based platforms. Analyst 136(1):29–41
Mura S, Nicolas J, Couvreur P (2013) Stimuli-responsive nanocarriers for drug delivery. Nat Mater 12:991–1003
Cheng R, Meng F, Deng C, Klok HA, Zhong Z (2013) Dual and multi-stimuli responsive polymeric nanoparticles for programmed site-specific drug delivery. Biomaterials 34:3647–3657
Place ES, Evans ND, Stevens MM (2009) Complexity in biomaterials for tissue engineering. Nat Mater 8:457–470
Tibbitt MW, Rodell CB, Burdick JA, Anseth KS (2015) Progress in material design for biomedical applications. Proc Natl Acad Sci 112:14444–14451
Howes PD, Chandrawati R, Stevens MM (2014) Colloidal nanoparticles as advanced biological sensors. Science 346:1247390–1247390
Su J, Chen F, Cryns VL, Messersmith PB (2011) Catechol polymers for pH-responsive, targeted drug delivery to cancer cells. J Am Chem Soc 133:11850–11853
Park I-K, Singha K, Arote RB, Choi Y-J, Kim WJ, Cho C-S (2010) pH-responsive polymers as gene carriers. Macromol Rapid Commun 31:1122–1133
Jochum FD, Theato P (2013) Temperature- and light-responsive smart polymer materials. Chem Soc Rev 42:7468–7483
Ercole F, Davis TP, Evans RA (2010) Photo-responsive systems and biomaterials: photochromic polymers, light-triggered self-assembly, surface modification, fluorescence modulation and beyond. Polym Chem 1:37–54
Chandrawati R, Städler B, Postma A, Connal LA, Chong SF, Zelikin AN, Caruso F (2009) Cholesterol-mediated anchoring of enzyme-loaded liposomes within disulfide-stabilized polymer carrier capsules. Biomaterials 30:5988–5998
Phillips DJ, Gibson MI (2012) Degradable thermoresponsive polymers which display redox-responsive LCST behaviour. Chem Commun 48:1054–1056
Chen W, Du J (2013) Ultrasound and pH dually responsive polymer vesicles for anticancer drug delivery. Sci Rep 3:2162–2162
Roy R, Yang J, Moses MA (2009) Matrix metalloproteinases as novel biomarkers and potential therapeutic targets in human cancer. J Clin Oncol 27:5287–5297
Park J, Yun HS, Lee KH, Lee KT, Lee JK, Lee S-Y (2015) Discovery and validation of biomarkers that distinguish mucinous and nonmucinous pancreatic cysts. Cancer Res 75:3227–3235
Khademhosseini A, Langer R (2007) Microengineered hydrogels for tissue engineering. Biomaterials 28:5087–5092
Ulijn RV, Bibi N, Jayawarna V, Thornton PD, Todd SJ, Mart RJ, Smith AM, Gough JE (2007) Bioresponsive hydrogels. Mater Today 10:40–48
Tibbitt MW, Anseth KS (2009) Hydrogels as extracellular matrix mimics for 3D cell culture. Biotechnol Bioeng 103:655–663
Singh SP, Schwartz MP, Tokuda EY, Luo Y, Rogers RE, Fujita M, Ahn NG, Anseth KS (2015) A synthetic modular approach for modeling the role of the 3D microenvironment in tumor progression. Sci Rep 5:17814–17814
McCall JD, Anseth KS (2012) Thiol–ene photopolymerizations provide a facile method to encapsulate proteins and maintain their bioactivity. Biomacromolecules 13:2410–2417. 45
Phelps EA, Enemchukwu NO, Fiore VF, Sy JC, Murthy N, Sulchek TA, Barker TH, García AJ (2012) Maleimide cross-linked bioactive PEG hydrogel exhibits improved reaction kinetics and cross-linking for cell encapsulation and in situ delivery. Adv Mater 24:64–70
Khetan S, Guvendiren M, Legant WR, Cohen DM, Chen CS, Burdick JA (2013) Degradation-mediated cellular traction directs stem cell fate in covalently crosslinked three-dimensional hydrogels. Nat Mater 12:458–465
Vandenbroucke RE, Libert C (2014) Is there new hope for therapeutic matrix metalloproteinase inhibition? Nat Rev Drug Discov 13:904–927
Chwalek K, Tsurkan MV, Freudenberg U, Werner C (2014) Glycosaminoglycan-based hydrogels to modulate heterocellular communication in in vitro angiogenesis models. Sci Rep 4:4414–4414
Turk BE, Huang LL, Piro ET, Cantley LC (2001) Determination of protease cleavage site motifs using mixture-based oriented peptide libraries. Nat Biotechnol 19:661–667
Hsu C-W, Olabisi RM, Olmsted-Davis EA, Davis AR, West JL (2011) Cathepsin K-sensitive poly(ethylene glycol) hydrogels for degradation in response to bone resorption. J Biomed Mater Res A 98:53–62
Brubaker CE, Messersmith PB (2011) Enzymatically degradable mussel-inspired adhesive hydrogel. Biomacromolecules 12:4326–4334
Vandamme TF, Lenourry A, Charrueau C, Chaumeil JC (2002) The use of polysaccharides to target drugs to the colon. Carbohydr Polym 48:219–231
Chourasia MK, Jain SK (2004) Polysaccharides for colon targeted drug delivery. Drug 11:129–148
Yao X, Liu Y, Gao J, Yang L, Mao D, Stefanitsch C, Li Y, Zhang J, Ou L, Kong D, Zhao Q, Li Z (2015) Nitric oxide releasing hydrogel enhances the therapeutic efficacy of mesenchymal stem cells for myocardial infarction. Biomaterials 60:130–140
Martino MM, Briquez PS, Ranga A, Lutolf MP, Hubbell JA (2013) Heparin-binding domain of fibrin(ogen) binds growth factors and promotes tissue repair when incorporated within a synthetic matrix. Proc Natl Acad Sci 110:4563–4568
Thornton PD, Billah SMR, Cameron NR (2013) Enzyme-degradable self-assembled hydrogels from polyalanine-modified poly(ethylene glycol) star polymers. Macromol Rapid Commun 34:257–262
Zelzer M, Todd SJ, Hirst AR, McDonald TO, Ulijn RV (2013) Enzyme responsive materials: design strategies and future developments. Biomater Sci 1:11–39
Wichterle O, Lim D (1960) Hydrophilic gels for biological use. Nature 185(4706):117–118
Lim F, Sun AM (1980) Microencapsulated islets as bioartificial endocrine pancreas. Science 210:908–910
Yannas IV, Lee E, Orgill DP, Skrabut EM, Murphy GF (1989) Synthesis and characterization of a model extracellular matrix that induces partial regeneration of adult mammalian skin. Proc Natl Aca Sci USA 86:933–937
Ratner B, Hoffman AS, Schoen F, Lemons JE (2004) Biomaterials science: introduction to materials in medicine, vol 2004, 2nd edn. Elsevier Academic Press, San Diego, pp 162–164
Silva GA, Czeisler C, Niece KL, Beniash E, Harrington DA, Kessler JA, Stupp SI (2004) Selective differentiation of neural progenitor cells by high-epitope density nanofibers. Science 303:1352–1355
Banwell EF, Abelardo ES, Adams DJ, Birchall MA, Corrigan A, Donald MA, Kirkland M, Serpell LC, Butler MF, Woolfson DN (2009) Rational design and application of responsive alpha-helical peptide hydrogels. Nat Mater 8:596–600
Kiyonaka S, Sada K, Yoshimura I, Shinkai S, Kato N, Hamachi I (2004) Semi-wet peptide/protein array using supramolecular hydrogel. Nat Mater 3(1):58–64
Lutolf M, Hubbell J (2005) Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering. Nat Biotechnol 23:47–55
Engler AJ, Sen S, Sweeney HL, Discher HL (2006) Matrix elasticity directs stem cell lineage specification. Cell 126:677–689
Ehrbar M, Rizzi SC, Schoenmakers RG, Miguel BS, Hubbell JA, Weber FE, Lutolf MP (2007) Biomolecular hydrogels formed and degraded via site-specific enzymatic reactions. Biomacromolecules 8:3000–3007
Ratner BD, Hoffman AS, Schoen FJ, Lemons JE (2004) Biomaterials science: introduction to materials in medicine, 2nd edn. Elsevier Academic Press, San Diego
Koutsopoulos S, Unsworth LD, Nagai Y, Zhang S (2009) Controlled release of functional proteins through designer self-assembling peptide nanofiber hydrogel scaffold. Proc Natl Acad Sci U S A 106:4623–4628
Chen L, Morris K, Laybourn A, Elias D, Hicks MR, Rodger A, Serpell L, Adams DJ (2009) Self-assembly mechanism for a naphthalene−dipeptide leading to hydrogelation. Langmuir 26:5232–5242
Soppimath K, Aminabhavi T, Dave A, Kumbar S, Rudzinski W (2002) Stimulus-responsive “smart” hydrogels as novel drug delivery systems. Drug Dev Ind Pharm 28:957–974
Walsh C (2001) Enabling the chemistry of life. Nature 409:226–231
Yang ZM, Liang GL, Guo ZH, Xu B (2007) Intracellular hydrogelation of small molecules inhibits bacterial growth. Angew Chem Int Ed 46:8216–8219
West JL, Hubbell JA (1999) Polymeric biomaterials with degradation sites for proteases involved in cell migration. Macromolecules 32:241–244
Reches M, Gazit E (2003) Casting metal nanowires within discrete self-assembled peptide nanotubes. Science 300:625–627
Ozbas B, Kretsinger J, Rajagopal K, Schneider JP, Pochan DJ (2004) Salt-triggered peptide folding and consequent self-assembly into hydrogels with tunable modulus. Macromolecules 37:7331–7337
Tang C, Smith AM, Collins RF, Ulijn RV, Saiani A (2009) FMOC-diphenylalanine self-assembly mechanism induces apparent pKa shifts. Langmuir 25:9447–9453
Hong H, Mai Y, Zhou Y, Yan D, Chen Y (2007) Synthesis and supramolecular self-assembly of thermosensitive amphiphilic star copolymers based on a hyperbranched polyether core. J Polym Sci A 46:668–681
Yang Z, Gu H, Fu D, Gao P, Lam JK, Xu B (2004) Enzymatic formation of supramolecular hydrogels. Adv Mater 16:1440–1444
Toledano S, Williams RJ, Jayawarna V, Ulijn RV (2006) Enzyme-triggered self-assembly of peptide hydrogels via reversed hydrolysis. J Am Chem Soc 128:1070–1071
Xu B (2009) Gels as functional nanomaterials for biology and medicine. Langmuir 25:8375–8377
Yang Z, Xu K, Guo Z, Guo Z, Xu B (2007) Intracellular enzymatic formation of nanofibers results in hydrogelation and regulated cell death. Adv Mater 19:3152–3156
Hirst AR, Roy S, Arora M, Das AK, Hodson N, Murray P, Marshall S, Javid N, Sefcik J, Boekhoven J, van Esch JH, Santabarbara S, Hunt NT, Ulijn RV (2010) Biocatalytic induction of supramolecular order. Nat Chem 2:1089–1094
Williams RJ, Smith AM, Collins R, Hodson N, Das AK, Ulijn RV (2008) Enzyme-assisted self-assembly under thermodynamic control. Nat Nanotechnol 4:19–24
Das AK, Hirst AR, Ulijn RV (2009) Evolving nanomaterials using enzyme-driven dynamic peptide libraries (eDPL). Faraday Discuss 143:293–303
Sadownik JW, Ulijn RV (2010) Locking an oxidation-sensitive dynamic peptide system in the gel state. Chem Commun 46:3481–3483
Ryan DM, Nilsson BL (2012) Self-assembled amino acids and dipeptides as noncovalent hydrogels for tissue engineering. Polym Chem 3:18–33
Adams DJ, Topham PD (2010) Peptide conjugate hydrogelators. Soft Matter 6:3707–3721
Yang Z, Liang G, Xu B (2008) Enzymatic hydrogelation of small molecules. Acc Chem Res 41:315–326
Collier JH, Messersmith PB (2003) Enzymatic modification of self-assembled peptide structures with tissue transglutaminase. Bioconjug Chem 14:748–755
Winkler S, Wilson D, Kaplan D (2000) Controlling beta-sheet assembly in genetically engineered silk by enzymatic phosphorylation/dephosphorylation. Biochemistry 39:12739–12746
Hirst AR, Coates IA, Boucheteau TR, Miravet JF, Escuder B, Castelletto V, Hamley IW, Smith DK (2008) Low-molecular-weight gelators: elucidating the principles of gelation based on gelator solubility and a cooperative self-assembly model. J Am Chem Soc 130:9113–9121
Adams DJ, Butler MF, Frith WJ, Kirkland M, Mullen L, Sanderson P (2009) A new method for maintaining homogeneity during liquid–hydrogel transitions using low molecular weight hydrogelators. Soft Matter 5:1856–1862
Sadownik JW, Leckie J, Ulijn RV (2011) Micelle to fibre biocatalytic supramolecular transformation of an aromatic peptide amphiphile. Chem Commun 47:728–730
Yang Z, Ho P-L, Liang G, Chow KH, Wang Q, Cao Y, Guo Z, Xu B (2007) J Am Chem Soc 129:266–267
Roy S, Ulijn RV (2010) Advances in polymer science. In: ARA P, Heise A (eds) Enzymatic polymerisation, vol 237. Springer, Berlin, pp 127–143
Thornton K, Smith A, Merry CLR, Ulijn RV (2009) Controlling stiffness in nanostructured hydrogels produced by enzymatic dephosphorylation. Biochem Soc Trans 37:660–664
Prabaharan M, Mano JF (2006) Stimuli-responsive hydrogels based on polysaccharides incorporated with thermo-responsive polymers as novel biomaterials. Macromol Biosci 6:991–1008
Santos SD, Chandravarkar A, Mandal B, Mimna R, Murat K, Saucede L, Tella P, Tuchscherer G, Mutter M (2005) Switch-peptides: controlling self-assembly of amyloid beta-derived peptides in vitro by consecutive triggering of acyl migrations. J Am Chem Soc 127(34):11888–11889
Yanlian Y, Ulung K, Xiumei W, Horii A, Yokoi H, Shuguang Z (2009) Designer self-assembling peptide nanomaterials. Nanotechnol Today 4:193–210
Ehrbar M, Rizzi SC, Schoenmakers RG, San Miguel B, Hubbell JA, Weber FE, Lutolf MP (2007) Biomolecular hydrogels formed and degraded via site-specific enzymatic reactions. Biomacromolecules 8:3000–3007
Corbett PT, Leclaire J, Vial L, West KR, Wietor J-L, Sanders JKM, Otto S (2006) Dynamic combinatorial chemistry. Chem Rev 106(9):3652–3711
Rowan SJ, Cantrill SJ, Cousins GRL, Sanders JKM, Stoddart JF (2002) Dynamic covalent chemistry. Angew Chem Int Ed 41:898–952
Vegners R, Shestakova I, Kalvinsh I, Ezzell RM, Janmey PA (1995) Use of a gel-forming dipeptide derivative as a carrier for antigen presentation. J Pept Sci 1:371–378
Zhang Y, Gu H, Yang Z, Xu B (2003) Supramolecular hydrogels respond to ligand−receptor interaction. J Am Chem Soc 125(45):13680–13681
Hughes M, Frederix PWJM, Raeburn J, Birchall LS, Sadownik J, Coomer FC, Lin I-H, Cussen EJ, Hunt NT, Tuttle T, Webb SJ, Adams DJ, Ulijn RV (2012) Sequence/structure relationships in aromatic dipeptide hydrogels formed under thermodynamic control by enzyme-assisted self-assembly. Soft Matter 8:5595–5602
Hughes M, Xu H, Frederix PWJM, Smith AM, Hunt NT, Tuttle T, Kinloch IA, Ulijn RV (2011) Biocatalytic self-assembly of 2D peptide-based nanostructures. Soft Matter 7(21):10032–10038
Hughes M, Birchall LS, Zuberi K, Aitkin LA, Debnath S, Javid N, Ulijn RV (2012) Differential supramolecular organisation of fmoc-dipeptides with hydrophilic terminal amino acid residues by biocatalytic self-assembly. Soft Matter 8:11565–11574
Jayawarna V, Richardson SM, Hirst AR, Hodson NW, Saiani A, Gough JE, Ulijn RV (2009) Introducing chemical functionality in FMOC-peptide gels for cell culture. Acta Biomater 5(3):934–943
Ruoslahti E (1996) RGD and other recognition sequences for integrins. Annu Rev Cell Dev Biol 12:697–715
Hughes M, Debnath S, Knapp CW, Ulijn RV (2013) Antimicrobial properties of enzymatically triggered self-assembling aromatic peptide amphiphiles. Biomater Sci 1:1138–1142
Brake JM, Daschner MK, Luk Y-Y, Abbott NL (2003) Biomolecular interactions at phospholipid-decorated surfaces of liquid crystals. Science 302:2094–2097
Lin IH, Birchall LS, Hodson N, Ulijn RV, Webb SJ (2013) Interfacing biodegradable molecular hydrogels with liquid crystals. Soft Matter 9:1188–1193
Gao Y, Kuang Y, Guo Z-F, Guo Z, Krauss IJ, Xu B (2009) Enzyme-instructed molecular self-assembly confers nanofibers and a supramolecular hydrogel of taxol derivative. J Am Chem Soc 131(38):13576–13577
Williams RJ, Hall TE, Glattauer V, White J, Pasic PJ, Sorensen AB, Waddington L, McLean KM, Currie PD, Hartley PG (2011) The in vivo performance of an enzyme-assisted self-assembled peptide/protein hydrogel. Biomaterials 32:5304–5310
Andrieu J, Kotman N, Maier M, Mailänder V, Strauss WSL, Weiss CK, Landfester K (2012) Live monitoring of cargo release from peptide-based hybrid nanocapsules induced by enzyme cleavage. Macromol Rapid Commun 33(3):248–253
Fuchs AV, Kotman N, Andrieu J, Mailander V, Weiss CK, Landfester K (2013) Enzyme cleavable nanoparticles from peptide based triblock copolymers. Nanoscale 5(11):4829–4839
Baier G, Cavallaro A, Vasilev K, Mailänder V, Musyanovych A, Landfester K (2013) Enzyme responsive hyaluronic acid nanocapsules containing polyhexanide and their exposure to bacteria to prevent infection. Biomacromolecules 14(4):1103–1112
Lin C-C (2015) Recent advances in crosslinking chemistry of biomimetic poly(ethylene glycol) hydrogels. RSC Adv 5:39844–39853
Dalby MJ, Gadegaard N, Tare R, Andar A, Riehle MO, Herzyk P, Wilkinson CD, Oreffo RO (2007) The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder. Nat Mater 6:997–1003
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Reduwan Billah, S.M., Mondal, M.I.H., Somoal, S.H., Nahid Pervez, M., Haque, M.O. (2018). Enzyme-Responsive Hydrogels. In: Mondal, M. (eds) Cellulose-Based Superabsorbent Hydrogels. Polymers and Polymeric Composites: A Reference Series. Springer, Cham. https://doi.org/10.1007/978-3-319-76573-0_62-1
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