Abstract
The low level red light (LLRL) is increasingly being used in many fields of medicine, like wound healing and regeneration of damaged tissue. Although the positive effects of LLLT have been reported the effects of red light on nanoscale features of tissues still remain unknown and little is known about LLRL-collagen interactions. Thus, in this study, thin collagen films were formed as extracellular matrix (ECM) models and irradiated so as to investigate the influence of LLRL irradiation on collagen topography, mechanical properties and the influence in fibroblasts response. The alterations on topography and collagen Young’s modulus were recorded using Atomic Force Microscopy (AFM). Furthermore, fluorescence microscopy was applied for studying the fibroblast response cultured on the collagen models. The results demonstrated that LLRL had minor effects on collagen topography. However, it was demonstrated that the LLRL altered the mechanical properties of the fibrils (the standard mechanical pattern due to the D – band periodicity was affected). Additionally, it was shown that fibroblasts cultured on LLRL-irradiated collagen thin films responded to LRLL. The above results provide new insights into the underlying nanoscale mechanisms of LLRL.
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Stylianou, A., Kontomaris, S.V., Yova, D. (2016). Probing Collagen Nanocharacteristics After Low-Level Red Laser Irradiation. In: Kyriacou, E., Christofides, S., Pattichis, C. (eds) XIV Mediterranean Conference on Medical and Biological Engineering and Computing 2016. IFMBE Proceedings, vol 57. Springer, Cham. https://doi.org/10.1007/978-3-319-32703-7_53
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DOI: https://doi.org/10.1007/978-3-319-32703-7_53
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