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The Investigation of Glucosinolates by Mass Spectrometry

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Glucosinolates

Part of the book series: Reference Series in Phytochemistry ((RSP))

Abstract

There is an ever-increasing interest in the biological effects of glucosinolates (GSLs), due to their anticarcinogenic properties and potential to contribute to dietary phytonutrient consumption, thereby improving human health and well-being. Cruciferous vegetables are unique in that they are rich sources of GSLs. Several epidemiological studies have shown that a high intake of cruciferous vegetables, for example, cabbage, broccoli, or Brussels sprouts, has beneficial influence on human health. A new window of investigations has been opened by mass spectrometry (MS) and its combination with the various chromatographic techniques has proved to be highly successful to gain reliable data about the presence and abundance of GSLs in vegetables. The purpose of this chapter is focused on the methods that are currently available for their qualitative and quantitative analysis by MS and tandem MS (MS/MS). Emphasis is placed on the description and value of existing methods as well as on the many MS applications reported for GSL analysis.

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Abbreviations

APCI:

Atmospheric pressure chemical ionization

CE:

Capillary electrophoresis

CID:

Collision-induced dissociation

CZE:

Capillary zone electrophoresis

DART:

Direct analysis in real time

ESI:

Electrospray ionization

FAB:

Fast atom bombardment

GC:

Gas chromatography

GLC:

Gas–liquid chromatography

GSLs:

Glucosinolates

HPCE:

High-performance capillary electrophoresis

HPLC:

High-performance liquid chromatography

HRMS:

High-resolution mass spectrometry

IRMPD:

Infrared multiphoton dissociation

ITCs:

Isothiocyanates

ITMS:

Ion trap mass spectrometry

LC:

Liquid chromatography

LC-ESI:

Liquid chromatography–electrospray ionization

MALDI:

Matrix-assisted laser desorption/ionization

MS/MS:

Tandem mass spectrometry

MECC:

Micellar electrokinetic capillary chromatography

MRM:

Multiple reaction monitoring

MS:

Mass spectrometry

MSI:

Mass spectrometry imaging

NMR:

Nuclear magnetic resonance

RP-HPLC:

Reversed-phase high-performance liquid chromatography

SIM:

Selected ion monitoring

SRM:

Selected reaction monitoring

TIC:

Total ion current

TOF:

Time of flight

XIC:

Extracted ion chromatogram

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Acknowledgments

This study was supported by the Università degli Studi di Bari Aldo Moro (Fondi di Ateneo 2012) and Università degli Studi della Basilicata.

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Authors and Affiliations

  1. Dipartimento di Scienze, Università degli Studi della Basilicata, Via dell’Ateneo Lucano, 10, 85100, Potenza, Italy

    Giuliana Bianco, Filomena Lelario & Sabino A. Bufo

  2. Scuola di Ingegneria, Dipartimento di Scienze, Università degli Studi della Basilicata, Via dell’Ateneo Lucano, 10, 85100, Potenza, Italy

    Raffaella Pascale

  3. Dipartimento di Chimica & Centro Interdipartimentale SMART, Università degli Studi di Bari Aldo Moro, Campus Universitario, Via E. Orabona, 4, 70126, Bari, Italy

    Tommaso R. I. Cataldi

Authors
  1. Giuliana Bianco
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  2. Raffaella Pascale
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  3. Filomena Lelario
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  4. Sabino A. Bufo
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  5. Tommaso R. I. Cataldi
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Corresponding authors

Correspondence to Giuliana Bianco , Raffaella Pascale , Filomena Lelario , Sabino A. Bufo or Tommaso R. I. Cataldi .

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Editors and Affiliations

  1. Institut des Sciences de la Vigne et du Vin, University of Bordeaux 2, Bordeaux, France

    Jean-Michel Mérillon

  2. Botany Department, M.L.Sukhadia University, Udaipur, Rajasthan, India

    Kishan Gopal Ramawat

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© 2016 Springer International Publishing Switzerland

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Bianco, G., Pascale, R., Lelario, F., Bufo, S.A., Cataldi, T.R.I. (2016). The Investigation of Glucosinolates by Mass Spectrometry. In: Mérillon, JM., Ramawat, K. (eds) Glucosinolates. Reference Series in Phytochemistry. Springer, Cham. https://doi.org/10.1007/978-3-319-26479-0_12-1

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  • DOI: https://doi.org/10.1007/978-3-319-26479-0_12-1

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  • Publisher Name: Springer, Cham

  • Online ISBN: 978-3-319-26479-0

  • eBook Packages: Springer Reference Chemistry and Mat. ScienceReference Module Physical and Materials ScienceReference Module Chemistry, Materials and Physics

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