Abstract
In this study, chondrocytes isolated from bovine cartilage tissue were seeded in agarose gel and resultant chondrocyte- agarose constructs, a well-established experimental model to examine the effect of mechanical loadings on the chondrocyte metabolism, were cultured with a traction loading on the construct surface to examine its effect on the regeneration of the cartilaginous tissue by chondrocytes. Customdesigned mechanical loading equipment was developed to apply the traction loading on the upper surface of constructs being cultured in the CO2 incubator. After 2 or 3 weeks culture, quantities of glycosaminoglycan (GAG) molecules that proteoglycan, and type II collagen were determined, and immunofluorescent staining of keratin sulfate, a type of GAG, and type II collagen was performed to verify the chondrocyte biosynthesis of extra cellular matrix (ECM) and characterize the structure of elaborated cartilaginous tissue by confocal laser scanning microscopy (CLSM). Results indicated that the traction loading enhance ECM biosynthesis in the surface region of constructs and collagen rich layer covered with GAG rich superficial layer was formed in the articulation surface. Results of quantification for ECM molecules indicated that the production of type II collagen and GAG was more significant outside the slide track compared with inside the slide track.
Access provided by Autonomous University of Puebla. Download to read the full chapter text
Chapter PDF
Similar content being viewed by others
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer International Publishing Switzerland
About this paper
Cite this paper
Keisuke, F., Seiji, O., Yoshinori, S. (2014). Development of Cartilaginous Tissue in Chondrocyte-Agarose Construct Cultured under Traction Loading. In: Goh, J. (eds) The 15th International Conference on Biomedical Engineering. IFMBE Proceedings, vol 43. Springer, Cham. https://doi.org/10.1007/978-3-319-02913-9_67
Download citation
DOI: https://doi.org/10.1007/978-3-319-02913-9_67
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-02912-2
Online ISBN: 978-3-319-02913-9
eBook Packages: EngineeringEngineering (R0)