Abstract
Dental enamel, which is the most highly mineralized tissue in the human body, is critically important for the protection of the underlying tooth structures throughout a lifetime. When enamel is absent or poorly formed due to genetic defects, called amelogenesis imperfecta (AI), or through environmental or epigenetic effects such as fluorosis, or lost due to dental caries, the entire tooth structure may be compromised. Ameloblasts differentiate through secretory, transition, and maturation stages to generate the fully mineralized enamel matrix. Enamel matrix proteins produced during the secretory stage undergo limited hydrolyzed by matrix metalloproteinase 20 (MMP20) to initiate crystal growth. Then during maturation, the remaining matrix proteins are completely hydrolyzed by kallikrein 4 (KLK4). The protein fragments are endocytosed and this allows complete mineralization of the enamel space. Enamel mineralization is unique in that it requires pH cycling, in which the ameloblasts modulate the matrix pH between an acidic and neutral pH. In this chapter, Part 1 is an overview of enamel formation, and how pH cycling influences amelogenin hydrolysis by KLK4 and enamel mineralization. Part 2 describes how ion-pumps and transporters regulate the enamel matrix pH as mineralization occurs.
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Keywords
- Tooth
- Enamel matrix
- Enamel maturation
- Ameloblasts
- Amelogenin
- MMP20
- KLK4
- pH cycling
- Enamel mineralization
- Ion transport
1 Part 1: Secretion of Matrix Proteins, Enamel Mineralization, and pH Regulation
1.1 Introduction
In the secretion stage of enamel formation, epithelial-derived ameloblasts secrete matrix proteins, to form a matrix with a cheese-like texture (Aoba and Moreno 1987; Smith and Nanci 1989). Enamel crystal formation is initiated in this matrix, and the crystals grow primarily in length and reach their full length at the end of the secretory stage. The secretory stage is followed by the transition stage, where the protein components in the enamel matrix dramatically decrease while the mineral components rapidly increase, resulting in a chalky appearing enamel matrix (Daculsi et al. 1984; Simmer et al. 2009). This transition leads to the maturation stage where the crystals continue to thicken and widen as proteins are simultaneously removed after degradation and replaced by hydroxyapatite (HAP) mineral. Following maturation, tooth enamel is a highly mineralized extracellular biomaterial, consisting of approximately 96–98% minerals and only 2–4% organic materials and water (He et al. 2011; Robinson 2014).
1.2 Secretory Stage Enamel
Enamel proteins are synthesized by the cells of the enamel organ, including ameloblasts. Ameloblasts elongate and form Tomes’ processes, through which enamel proteins are secreted in a prism-like structure, on top of the dentin matrix (Kallenbach 1973). The full thickness of the enamel matrix is established by matrix proteins that include amelogenins, ameloblastin, enamelin, tuftelin, and keratins (Robinson et al. 1998).
Amelogenins, which are transcribed from multiple alternatively spliced variants of the amelogenin gene, are the primary structural proteins, constituting 90–95% of total proteins in the secretory enamel protein matrix (Gibson 2011). Amelogenins play a central role in crystal growth and enamel thickness (Gibson et al. 2001; Robinson et al. 1998). The amelogenin knockout mouse has an enamel layer that is much thinner than that in the wild-type mouse, and the enamel crystals are disorganized, deformed, and disoriented (Gibson et al. 2001). The absence of ameloblastin (Ambn) results in a detachment of ameloblasts from the underlying matrix at the start of the secretory stage and generation of a thin enamel with an irregular prism pattern (Wazen et al. 2009). When enamelin is absent, ameloblasts are unable to adhere to the underlining enamel surface, and they prematurely undergo apoptosis (Hu et al. 2011). Mutations in keratin 75 are associated with an increased incidence of dental caries (Duverger et al. 2014).
The secretory matrix also contains proteinases, including matrix metalloproteinases (MMPs) (Bartlett 2013; Wöltgens et al. 1991), MMP2, MMP3, MMP9, MMP12, MMP13 and MMP20 (Bartlett 2013; DenBesten and Heffernan 1989; Goldberg et al. 2003; Llano et al. 1997; Wöltgens et al. 1991). MMP20 is the predominant enamel matrix proteinase in the secretory stage and cleaves enamel matrix proteins immediately after their secretion (Bartlett et al. 1998; Simmer and Hu 2002).
Mineral formed in the enamel matrix is initially amorphous calcium, which subsequently transforms to hydroxyapatite (HAP) (Beniash et al. 2009). The phosphorylated N terminal serine 16 on alternatively spliced amelogenins enhances the stabilization of amorphous calcium phosphate (ACP) mineral precursors and modulates the timing of conversion of ACP to apatite mineral (Shin et al. 2020). At the end of the secretory stage, long thin crystals of HAP extend the full length of the enamel matrix. The secretory stage ends as capillaries invaginate into the stellate reticulum layer of the enamel organ, overlying ameloblasts. Up to 50% of the ameloblasts undergo apoptosis during this transition so that there is no longer one ameloblast overlaying each enamel prism. The ameloblasts then further differentiate into maturation stage ameloblasts (Smith 1998).
1.3 Maturation Stage Enamel
Maturation ameloblasts cyclically modulate between ruffle-ended (RE) cells and smooth-ended (SE) cells (Fig. 11.1). Matrix proteins secreted at the maturation stage include amelotin (AMTN), odontogenic ameloblast-associated protein (ODAM) (Moffatt et al. 2008), and secretory calcium-binding phosphoprotein proline-glutamine rich 1 (SCPPPQ1). These proteins are thought to participate in structuring an extracellular matrix with the distinctive capacity of attaching epithelial cells to mineralized surfaces (Fouillen et al. 2017), and the formation of the final layer of aprismatic enamel (Abbarin et al. 2015).
Kallikrein 4 (KLK4), a serine proteinase previously named enamel matrix serine proteinase (EMSP1), first identified and purified from porcine enamel matrix by Simmer and colleagues (Simmer et al. 1998), is the predominant enamel matrix proteinase in the maturation stage (Simmer and Hu 2002). KLK4 hydrolyzes matrix proteins to create space for the mineralizing enamel crystals to expand in width.
1.4 Discovery of pH Cycling
Takano and coworkers first reported the visualization of ameloblast modulation on the enamel surface, by staining with the calcium-binding dye glyoxal bi (2-hydroxyanil) (GBHA). Enamel under SE stains red by GBHA, whereas enamel under RE stains a yellow/green color, or is unstained (Takano et al. 1982a) (see Fig.11.1). However, an unsolved question at that time was that Ca45 uptake is not correlated with RE and SE morphologies (Takano et al. 1982b).
Sasaki stained the enamel surface with a pH indicator dye and found that the enamel had alternating acidic and neutral pH. Furthermore, these pH cycles of acidic (5.8–6.0) and neutral (7.0–7.2) (see Fig. 11.1) were located at the same area stained by GBHA, indicating that enamel under RE ameloblasts was more acidic, and was more neutral under SE ameloblasts (Sasaki et al. 1991b) (see Figs. 11.1 and 11.2).
The correlation of enamel staining by GBHA and pH indicator dye later became clearer, as it was noted that GBHA stains poorly at acid pH. Therefore, ionic calcium staining by GBHA in the developing enamel matrix is also pH dependent. Calcein, another dye that stains ionic calcium, is also pH sensitive, and so has a similar property to that of GBHA, of only staining the neutral enamel matrix (Josephsen 1983; Picard et al. 2000).
Takagi et al. further characterized the profiles of the enamel matrix proteins collected from enamel either with acidic or neutral pH (Takagi et al. 1998). They discovered that the neutral enamel matrix contains relatively intact amelogenin and enamelin proteins, while the acid zones contain mostly lower molecular weight amelogenins and enamelin fragments. This suggests that amelogenin hydrolysis is more effective in the acidic conditions underlying RE ameloblasts.
We do not yet understand what drives the cyclic modulation of maturation ameloblasts. However, we do know that ion transporters localized to the ameloblast apical plasma membranes transport calcium into the enamel matrix to form HAP, while modulating matrix pH to direct this process. Bicarbonate ion transporters, including Ae2 and NBCe1, neutralize the acidified matrix. Matrix acidification is driven by the formation of HAP crystals which release protons into the matrix (Bronckers 2017; Smith 1998). Matrix acidification may also be controlled by proton secretion by V-ATPase translocated to the RE apical plasma membrane (Damkier et al. 2014; Toei et al. 2010). Modulations of ion transporter activities by either genetic defects, or environmental factors, such as excess fluoride, affect pH cycling and enamel matrix formation (Bronckers et al. 2015; DenBesten and Li 2011). Therefore, pH cycling, which is related to both matrix protein endocytosis and matrix mineralization is a unique mechanism that drives the formation of biomineralized enamel.
1.5 Amelogenin-Mediated Enamel Matrix Mineralization
The amelogenin protein structure is highly pH dependent. At pH 3.0 amelogenin is globally unstructured, while at pH 5.6, it forms elaborated structures with increased oligomerization and nanosphere formation. At pH 7.2 amelogenin assembles into highly organized structures, binding together to form branched chains (Beniash et al. 2012). In addition, at neutral pH, changes in electrolyte concentration dramatically affect protein adsorption onto the HAP surface (Shimabayashi et al. 1997).
The C terminus of amelogenin is a primary site for amelogenin binding to HAP. Mice lacking the 13 terminal amino acids have poorly formed enamel (Pugach et al. 2010). Glutamate-containing proteins interact strongly with Ca2+ ions on the surface of the HAP crystal, and acidic amino acids have been considered as major determinants of protein binding (Jaeger et al. 2005). It is therefore likely that the acid glutamates and aspartate (2 each) in the amelogenin C terminus may facilitate binding to the HAP surface.
When amelogenins assemble in a neutral aqueous environment, the hydrophilic C termini extend on the outside of the amelogenin nanostructures (Margolis et al. 2006). The exposed C terminal amino acids can bind to the apatite crystal surfaces, and also to positively charged protons and calcium in solution (Le et al. 2006; Ryu et al. 1998). Moreover, the positively charged groups of amelogenin (such as amine groups at the N-terminus and side chains) may also contribute to the apatite binding through their interactions with the negatively charged groups, such as phosphates, on the HAP surface.
The concentration of amelogenin influences HAP crystal growth. HAP crystal formation requires a supersaturated calcium phosphate environment (Eanes 1976; Eanes et al. 1965). In solution, amelogenins inhibit crystal growth in the presence of high concentrations of calcium and phosphate (Aoba et al. 1989; Iijima and Moradian-Oldak 2004). However, Beniash and co-workers observed that pre-assembled full-length amelogenin (amelogenin lacking the exon 4 transcript) increases the size of formed crystals at low concentrations of calcium and phosphate (1.5 and 2.5 mM) (Beniash et al. 2005). In the presence of calcium and phosphate concentrations similar to those measured in enamel fluid (0.5 and 2.5 mM, respectively); crystals do not grow well on an apatite surface (Aoba and Moreno 1987; Habelitz et al. 2005). However, with the addition of amelogenin at low concentration (0.4 mg/ml), a few nanospheres assemble with minimal crystals formation, and at higher concentrations of amelogenin (1.6 mg/ml) more nanospheres assemble and longer fibrous crystals are formed (Habelitz et al. 2004).
At these higher amelogenin concentrations, amelogenin nanospheres assemble as a monolayer on the crystal surface. It appears that this monolayer of amelogenin nanospheres functions differently from amelogenin monomers in solution (Aoba et al. 1989) or in an amelogenin gel (Iijima et al. 2002). The amelogenin monolayer nanostructure may create a highly charged local environment to attract calcium and phosphate ions to form a saturated niche on the crystal surface for crystal growth.
Additional multilayers of amelogenin nanospheres physically protect the fragile crystals, but they also inhibit further crystal growth. Therefore, the layer of amelogenin adsorbed on the crystal surface needs to be preferentially removed to release the space for crystal growth.
1.5.1 Disassembly of Amelogenin Bound on HAP Crystals
When bound to solid surfaces, protein conformations usually change (Hlady and Buijs 1996). Proteins, such as amelogenin, which can easily adsorb onto solid surfaces, have large changes in conformation upon binding, and less internal stability (Gray 2004). Tarasevich et al. found that at neutral pH, self-assembled amelogenin that was bound onto a single fluoroapatite (FAP) crystal, formed much smaller structures on the FAP surfaces, than the original nanospheres present in solution. Their studies provide strong evidence that amelogenin nanospheres undergo possible quaternary structural changes upon interacting with apatite surfaces (Tarasevich et al. 2009a, b). These adsorption-induced conformational changes of the amelogenin protein may further alter the subsequent interactions between amelogenin and proteinases.
Tao et al. further quantitatively analyzed the dynamics of this adsorption-induced amelogenin nanosphere disassembly. They reported that the amelogenin nanospheres disassembled onto the HAP surface to form oligomeric adsorbates (25-mer), the subunits of the larger nanosphere. They concluded that a surface-triggered disassembly mechanism actually reversed the process of oligomer nanosphere self-assembly (Tao et al. 2015). This supports the possibility that amelogenin–crystal interactions are involved in determining the extent to which oligomers adsorb, assemble, and disassemble.
Chen et al. used in situ atomic force microscopy (AFM) to show that on a positively charged surface, amelogenin first assembles as a relatively uniform population of decameric oligomers, and then becomes two main populations: higher-order assemblies of oligomers and amelogenin monomers. On negatively charged surfaces, amelogenin nanostructure disassembles into a film of monomers (Chen et al. 2011) (see Fig. 11.3). An acidic pH of the enamel matrix, resulted from HAP formation, may further disassemble amelogenin nanospheres (Beniash et al. 2012).
Therefore, a possible sequence for amelogenin and pH-mediated enamel matrix mineralization is as follows. Amelogenins bind to HAP crystals and then disassemble to form monolayers that attract calcium and phosphate ions to form a saturated niche on the crystal surface for crystal growth. As crystal growth is initiated, the matrix begins to acidify and the N-terminus of amelogenins, which contain more positively charged histidines, reduces its binding affinity to HAP crystals, while the hydrophilic amelogenin C termini containing more acidic amino acids, still hangs on the surface of crystals. This allows more access sites for proteinases, facilitating the digestion of bound amelogenin. As the matrix neutralizes, HAP synthesis begins, and as the crystals grow, the surface of newly formed crystals will contact another layer of proteins in the enamel matrix. This then initiates amelogenin disassembly again, and the cycle repeats itself during enamel maturation.
1.6 Enamel Matrix Proteinases, MMP-20, and KLK4
The transition of enamel matrix from protein-dominated content to more than 95% mineralized tissue requires hydrolysis of matrix proteins to allow continued crystal growth as enamel matures. MMP20 and KLK4 are two major proteinases involved in the hydrolysis of amelogenins and other enamel matrix proteins, to provide space for enamel crystals growth (Bartlett et al. 1998; Bartlett and Simmer 1999; Caterina et al. 1999; Fukae et al. 1998; Hu et al. 2002; Ryu et al. 2002).
MMP20, first reported by Bartlett and co-workers (Bartlett et al. 1998; Llano et al. 1997), belongs to the MMP superfamily and shares a similar structure with most other family members (Massova et al. 1998). Recombinant porcine, mouse, bovine, and human MMP20s have been expressed, purified, and activated in vitro and can hydrolyze both recombinant and native amelogenins (Li et al. 1999; Llano et al. 1997).
Amelogenin hydrolysis is initiated in secretory stage enamel at its C-terminal telopeptide. The most prevalent splice variants code the full length 25 kDa amelogenin that lacks exon 4 and the leucine-rich amelogenin polypeptide (LRAP) precursor. The 25 kDa amelogenin is cleaved into products of 20 kDa amelogenin and then a 5.0 kDa tyrosine-rich amelogenin polypeptide (TRAP). Similarly, the LRAP precursor is cleaved at the C terminus to form a 6.5 kDa LRAP (Fincham et al. 1981, 1989). Twenty kDa amelogenin, LRAP, and TRAP are the three major hydrolytic products in the secretory enamel protein matrix (Brookes et al. 1995).
KLK4 is upregulated during the transition stage. KLK4 rapidly cleaves amelogenins at multiple sites, accelerating amelogenin degradation during enamel maturation, to allow the final thickening of enamel crystals. The importance of KLK4 in the final stage of enamel mineralization is shown in the KLK4 knockout mouse, where the enamel layer is normal in thickness with an intact decussating structure but the crystals remain separated, resembling a pattern of uncooked angel hair spaghettis (Fig.11.4) (Bartlett and Simmer 1999; Ryu et al. 2002; Simmer et al. 2009).
1.6.1 Optimal Conditions for Proteinase Activity in the Enamel Matrix
In the secretory matrix, MMP-20 cleaves amelogenin at neutral pH (Fukae et al. 1998), whereas KLK4 has an optimal pH close to 6.1 (Lu et al. 2008). In vitro, optimal KLK4 activity against a peptide substrate occurs even lower, around pH 5, and at neutral pH, KLK4 activity was reduced by almost half (Fig. 11.5, unpublished data).
This acidic optimal pH for KLK4 suggests the importance of acidic conditions in the degradation of amelogenin and other enamel proteins during tooth enamel maturation (Sasaki et al. 1991a). In addition, MMP20 is reported to be able to activate KLK4 (Yamakoshi et al. 2013). Interestingly, some MMPs are reported to be activated by low pH, which plays an important role in caries formation (Amaral et al. 2018). Therefore, the sequential activation of KLK4 by MMP20 remaining in the maturation stage matrix may also contribute to the tooth enamel maturation.
The absorption of amelogenin onto hydroxyapatite also affects its hydrolysis. When amelogenin is absorbed onto hydroxyapatite it is readily hydrolyzed and removed by enamel matrix proteinases (88% for MMP20 and 98% for KLK4), at significantly higher rates as compared to hydrolysis of amelogenin in solution. There are also a greater number of cleavage sites as identified by LC-MALDI MS/MS, when amelogenin is hydrolyzed after absorption onto HAP as compared to those in solution (Zhu et al. 2014). These results suggest that the adsorption of amelogenin to HAP results in their preferential and selective degradation and removal from HAP by both MMP20 and KLK4. It may be that the disassembly of nanospheres into monomers at low pH and through amelogenin–crystal interactions results in more cleavage sites accessible to enzymes and lead to a higher rate of hydrolysis.
1.7 A Model for pH Cycling and KLK4-Regulated Enamel Maturation
How does pH cycling affect enamel matrix mineralization? A possible cyclic sequence of mineralization and protein removal is in Fig. 11.6.
Adsorption of amelogenins onto the crystal surface results in conformational changes of the bound proteins, which then disassemble into oligomers or monomers on the surface of HAP crystals. These conformational changes may both increase local supersaturation of calcium and phosphate at the HAP surface, and expose more amelogenin cleavage sites to proteinases.
The hydrolysis and removal of amelogenins from the crystal surface open up the space surrounding the crystals, possibly with rapid precipitation of amorphous calcium phosphate (ACP), which then subsequently transforms to HAP. HAP formation releases protons contributing to matrix acidification, which then enhances amelogenin disassembly and KLK4 activity resulting in more protein hydrolysis. When the pH is regulated by ameloblasts to neutral, nascent crystals growing into this new space come into contact with the next layer of amelogenin nanospheres, and yet another cycle of the interaction-mediated preferential removal of bound amelogenin and crystal growth is initiated. The cycles of binding-growth-hydrolysis repeat until most of the matrix proteins are removed and the HAP crystals grow to fill up the entire enamel space to form the hardest tissue in our body.
2 Part 2: Regulations of Ion-Pumps, Transporters and Carbonic Anhydrases (CAs) Involved in Enamel Mineralization and pH Modulation
Maturation stage ameloblasts are responsible for the transport of 86% of minerals that are required for enamel matrix biomineralization (Smith 1998). As maturation stage ameloblasts cycle between smooth- and ruffle-ended ameloblasts, the expression and spatial cellular distribution of ion-transporters and carbonic anhydrases modulate to direct extracellular matrix pH cycling (Smith et al. 1996) while also transporting calcium and phosphate into the enamel matrix for crystal growth. Genes coding ion pumps (V-type-H+ATPase), transporters (CFTR, SLC26A, AE2, NHE1, NBCe1, NKCC1), and CAs (CA2 and CA6) are upregulated in ruffle-ended ameloblasts (Lacruz et al. 2011; Kim and Hong 2018). Reciprocal interactions between maturation ameloblasts and underlying enamel matrix contribute to the changes in gene expression between ruffle-ended and smooth-ended ameloblast in this unique process of enamel biomineralization.
2.1 Regulation of Matrix pH
2.1.1 Matrix Acidification
Cyclic acidification of the enamel matrix is associated with matrix mineralization (see Part 1). Matrix acidification occurs with the formation of hydroxyapatite crystals, which generate protons as the crystals form, and may also possibly be enhanced by extrusion of protons into the extracellular enamel matrix by V-H+ATPase and NHE1 (SLC(A1).
V-Type H+-ATPase subunits (Atp6v0d2, Atp6v1b2, Atp6v1c1 and Atp6v1e1) are highly upregulated in maturation stage ameloblasts. These subunits are involved in vesical acidification required for endocytosis of matrix proteins. The presence of subunits ATP6v1c1, ATP6v1e1, and Atp6v0a1 at the apical membrane of ruffle-ended maturation ameloblasts (Damkier et al. 2014; Josephsen et al. 2010; Sarkar et al. 2016), suggests an additional possibility, that these subunits of V-type H+-ATPase may export protons to assist the enamel matrix acidification. However, only a relatively small amount of bicarbonate is available for buffering the protons that are rapidly released as hydroxyapatite crystals form (Smith 1998). Thus, protons adding to the enamel matrix through V-type H+-ATPase may have only a limited role in further acidifying the enamel matrix.
NHE1, which is encoded by the SLC9A1 gene extrudes protons into the matrix as it mediates Na+ influx and H+ efflux. NHE1 as an acid extruder was identified in the lateral membrane during both the secretory and the maturation stages of ameloblasts (Josephsen et al. 2010). SLC9A1 is widely expressed with a preferential expression in the kidney, intestine, and liver, and NHE1 protein has a central role in regulating pH homeostasis, cell migration, and cell volume.
Calcium Transport
Acidification of the enamel matrix occurs when calcium and phosphate are transported into the matrix to form hydroxyapatite crystals. Maturation stage ameloblasts are responsible for the deposition of more than 70% of the calcium in enamel tissues (Smith 1998). This transport of calcium by the ameloblasts requires tight control of intracellular calcium concentrations ([Ca2+]) to avoid Ca2+ toxicity (Hubbard 2000).
Calcium transport into ameloblasts is mediated by store-operated calcium entry (SOCE) (Eckstein et al. 2017; Nurbaeva et al. 2017), ORAI, as well as by the cation channel TRPM7 (transient receptor potential cation channel subfamily M) (Faouzi et al. 2017; Nakano et al. 2016) and connexin 43 (Al-Ansari et al. 2018; Toth et al. 2010). The majority of Ca2+ is stored in the endoplasmic reticulum lumen, and the rest of excess Ca2+ binds to cytoplasmic calcium-binding proteins, including calbindin-D9k, -D28k, calmodulin (Berdal et al. 1993; Berdal et al. 1996; Hubbard 1995).
Calcium extrudes from ameloblasts into the enamel matrix through the basolateral and apical transmembrane Ca2+ pump, cotransporters/ion-exchangers, including Ca2+-ATPase (PMCA), potassium-independent Na+/Ca2+ (NCX) and potassium-dependent Na+/Ca2+ exchangers (NCKX) (Nurbaeva et al. 2017). Among the four types of PMCA subunits, PMCA-1 (ATP2B1) and PMCA-4 (ATP2B4) are the predominant isoforms of PMCA in human and mouse ameloblasts. Though the expression levels of these two isoforms remain comparable between secretory and maturation stage ameloblasts (Borke et al. 1993; Borke et al. 1995; Zaki et al. 1996), in maturation stage ameloblasts PMCA has been immunolocalized at the apical surface (Salama et al. 1987; Zaki et al. 1996).
As compared to PMCA, ion exchangers NCX and NCKX have a higher turnover rate of delivering calcium (Eisenmann et al. 1982), which is critical for providing a large amount of calcium for the rapid growth of hydroxyapatite crystals. The NCX isoform exchanges one Ca2+ outward for three Na+ inward (Yu and Choi 1997). Ncx1 and Ncx3 have been found to be equally expressed by secretory and maturation stage ameloblasts (Lacruz et al. 2012), suggesting that NCXs may act as housekeeping calcium transport in both secretory and maturation stage ameloblasts.
Whole transcriptome analysis shows that NCKX4 is the dominant isoform of the NCKX family expressed by ameloblasts, and is significantly upregulated in maturation ameloblasts as compared to secretory ameloblasts (Hu et al. 2012; Lacruz et al. 2012). NCKX4 can extrude one Ca2+ and one K+ in exchange for four Na+ inward (Lytton 2007). In humans, the NCKX4 gene has been identified as a causal gene of amelogenesis imperfecta (Jalloul et al. 2016; Parry et al. 2013; Smith et al. 2017).
Phosphate Transport
Hydroxyapatite has a calcium/phosphate ratio of 1.67, and therefore, also requires phosphate to be transported to the enamel matrix. A Na+-dependent Pi transporter such as NaPi-2b (SLC34A2), which is located in ruffle-ended ameloblasts, may operate in a coordinated way with NCKX4 to direct phosphate into the cells (Bronckers 2017; Bronckers et al. 2015).
2.1.2 Buffering to Neutralize Matrix pH
Secretory Stage
In the secretory stage enamel, where hydroxyapatite crystal formation is initiated to form long thin crystals, matrix acidification as a result of crystal formation, is likely buffered by the amelogenins in the secretory enamel matrix (Guo et al. 2015). Amelogenins are rich in histidine, which can absorb protons (Bansal et al. 2012; Simmer and Fincham 1995).
Maturation Stage
In the maturation stage, there are open junctions between smooth-ended ameloblasts, whereas ruffled-ended ameloblasts have tight apical junctions and open basal junctions (Skobe et al. 1985). The intercellular space between smooth-ended ameloblasts allows the free paracellular movement of fluid, ions, and small molecules, which might be able to correct the imbalance of pH immediately in the confined enamel space (Hanawa et al. 1990; Kawamoto and Shimizu 1997; McKee et al. 1986; Smith et al. 1987; Takano 1995; Takano et al. 1982b). This has been described as a “fluid flush” between smooth-ended ameloblasts, which brings in “base” and takes away “acid” to aid in the pH regulation activities.
In addition to this possibility, it is widely accepted that extrusion of bicarbonate by ameloblasts into the extracellular spaces buffers the intense acid loading during the rapid crystal growth in mineralizing enamel (Lacruz et al. 2010; Smith 1998; Varga et al. 2018; Yin and Paine 2017). Whole transcriptome analyses have shown that as compared to secretory ameloblasts, maturation ameloblasts have significantly upregulated genes involved in bicarbonate transport. These genes include: the chloride/bicarbonate channel CFTR (cystic fibrosis transmembrane conductance regulator); NBCe1 (sodium bicarbonate exchanger, solute carrier family 4 member 4); CA2 (carbonic anhydrase CAII) and CA6 (CAVI); AE2 (anion exchange protein 2) and the bicarbonate chloride exchangers SLC26A3/A4/A6/A7 (solute carrier 26, members A3, A4, A, and A7) (Bronckers 2017; Kim and Hong 2018; Lacruz et al. 2012; Simmer et al. 2014).
Ion Transporters
Ion transporters localized on apical ameloblast membranes include CFTR (Bronckers et al. 2010), SLC26 members (Yin et al. 2015), and CA (Bronckers 2017). CFTR likely contributes to bicarbonate transport in epithelial cells both directly by permeation through the channel, and indirectly by facilitating the function of Slc26 (Fong 2012). Using large-scale transcriptomic analysis, SLC26A4 has been shown as a target gene modulated by NaF and bisphenol in dental epithelia of rats (Jedeon et al. 2016b). To maintain bicarbonate supplies for extracellular transport, maturation-stage ameloblasts use carbonic anhydrases, CA2 and CA6 to catalyze the hydration of CO2. CA2 generates bicarbonate in the cytosol of ameloblasts (Josephsen et al. 2010; Lin et al. 1994; Toyosawa et al. 1996), and CA6, the secreted form of carbonic anhydrase, may generate bicarbonate in the enamel space (Smith et al. 2006).
Ion transporters localized on the basal ameloblast membranes include AE2, NBCe1, NHE1, and NKCC1. AE2 encoded by the SLC4A2 gene is a Cl−/HCO3− exchanger that exports bicarbonate into the extracellular space, in exchange for Cl− (Lyaruu et al. 2008; Paine et al. 2008). Bicarbonate can also be transported into ameloblasts by NBCe1, encoded by the SLC9A1 gene, which also mediates inward Na+ influx and H+ efflux (Josephsen et al. 2010; Lacruz et al. 2010; Paine et al. 2008). NKCC1, encoded by the SLC12A2 gene, mediates sodium and chloride transport and reabsorption.
pH Sensing Proteins
pH sensing proteins that may direct ameloblast regulation of matrix pH include the G-protein-coupled receptor 68 (GPR68). Defects in GPR68 are associated with amelogenesis imperfecta, suggesting a functional role of this pH sensor in ameloblast modulation of matrix pH cycling (Varga et al. 2018). GPR68 can also regulate steroid receptors, also present in maturation-stage ameloblasts (Houari et al. 2016). Steroid hormones and their receptors were reported to regulate CFTR, SLC26A6, NHE, and CA in several tissues (Gholami et al. 2013).
External Factors Regulating Ion Transport by Ameloblasts
Ameloblasts targeted by environmental toxicants and chemicals with endocrine-disrupting activities result in alterations in enamel formation (Babajko et al. 2017; Jedeon et al. 2013, 2016a, b) (see also Chap. 12). All of the transporters described in this chapter, with the possible exception of AE2, can be modulated by hormones or molecules with endocrine activity.
Hormonal changes that may affect enamel formation also include possible effects on the function of the HPA (hypothalamic–pituitary–adrenal) axis during enamel formation. Evidence for this possibility is the finding by Boyce et al., that children entering kindergarten who had increased salivary cortisol reactivity, had primary (deciduous) mandibular incisors with thinner and more hypomineralized enamel (Boyce et al. 2010). Cortisol reactivity in young children is associated with maternal prenatal stress (Luecken et al. 2013), and as the primary mandibular incisors are formed during the prenatal period of life, this suggests a possible link between alternations in HPA axis regulations and enamel formation.
2.2 Conclusions
Ameloblast Regulation of pH Cycling
Our understanding of how ameloblasts modulate and regulate pH cycling, as described in this chapter, has been advanced through immunolocalization of channels, transporters, and exchangers on ameloblast membranes, as well as in the use of cell culture models to investigate calcium transport and intracellular pH regulation (Bori et al. 2016; Bronckers 2017; Bronckers et al. 2015; Nurbaeva et al. 2015) (see Fig. 11.7).
However, much remains to be understood about the regulation of pH cycling by ameloblasts. As we further understand the mechanisms by which pH is regulated in enamel we will be better able to ensure optimal amelogenesis, enamel formation and mineralization.
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The work was supported by NIH/NIDCR fundings R01DE027971, R01DE025709 and R01DE027076.
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Li, W., Zhang, Y., Babajko, S., Besten, P.D. (2021). Enamel Matrix Biomineralization: The Role of pH Cycling. In: Goldberg, M., Den Besten, P. (eds) Extracellular Matrix Biomineralization of Dental Tissue Structures. Biology of Extracellular Matrix, vol 10. Springer, Cham. https://doi.org/10.1007/978-3-030-76283-4_11
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DOI: https://doi.org/10.1007/978-3-030-76283-4_11
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