Abstract
Plants activate a range of active defense strategies in response to biotic stresses. Systemic acquired resistance (SAR) occurs when the plants are triggered by a stimulus prior to infection by a plant pathogen able to reduce the severity of the disease. The most widely studied group of PGPB is plant growth-promoting rhizobacteria that colonize the root surface. This trait plays a crucial role in protecting plants from pathogen attack since poor colonization could cause decreased antagonistic activity. The present work aims to isolate, identify and select strains from the rhizosphere of strawberry in the Larache area, with potential application as a biocontrol agent against gray mold disease caused by Botrytis cinerea. First, the selected bacteria and the pathogen were characterized using 16S rDNA and ITS sequencing respectively, and then challenged in dual culture as antagonists to evaluate their effect on the growth of B. cinerea. Among the selected bacteria that inhibited the growth of B. cinerea in vitro dual culture, Bacillus amyloliquefaciens was able to associate to the external root surfaces, by forming biofilm, moreover, preliminary analyses showed that, upon attack with Botrytis cinerea, B. amyloliquefaciens primed strawberry’s plants, showed an enhanced expression of PR1 and β-1,3-glucanase (FaBG2-2) genes in comparison to non-primed plants. However, no expression of chitinase II genes (FaChi2-1, FaChi2-2) was observed. This “priming” effect indicated that B. amyloliquefaciens could be further studied for its biocontrol traits able to induce, once inoculated, defense-related gene expression earlier and stronger than in non-primed plants.
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References
Ahmad, F., Ahmad, I., Khan, M.S.: Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiol. Res. 163, 173–181 (2008)
Gray, E.J., Smith, D.L.: Intracellular and extracellular PGPR: commonalities and distinctions in the plant-bacterium signaling processes. Soil Biol. Biochem. 37, 395–412 (2005)
Saravanakumar, D., Vijayakumar, C., Kumar, N., Samiyappan, R.: PGPR-induced defense responses in the tea plant against blister blight disease. Crop Prot. 26, 556–565 (2007)
Van Loon, L.C., Bakker, P.A.H.M., Pieterse, C.M.J.: Systemic resistance induced by rhizosphere bacteria. Annu. Rev. Phytopathol. 36, 453–483 (1998)
El Hamdouni, E.M., Lamarti, A., Badoc, A.: In vitro germination of the achenes of Strawberry (Fragaria x ananassa Duch.) cvs. “Chandler” and “Tudla”. Bull. Soc. Pharm. Bordeaux 140, 31–42 (2001)
Favaro, G., Bogialli, S., Di Gangi, I.M., Nigris, S., Baldan, E., Squartini, A., et al.: Characterization of lipopeptides produced by Bacillus licheniformis using liquid chromatography with accurate tandem mass spectrometry. Rapid Commun. Mass Spectrom. 30, 2237–2252 (2016)
Navazio, L., et al.: A diffusible signal from arbuscular mycorrhizal fungi elicits a transient cytosolic calcium elevation in host plant cells. Plant Physiol. 144, 673–681 (2007)
Tortora, M.L., et al.: Protection of strawberry plants (Fragaria ananassa Duch.) against anthracnose disease induced by Azospirillum brasilense. Plant Soil 356, 279–290 (2012)
Tewari, S., Arora, N.K.: Multifunctional exopolysaccharides from Pseudomonas aeruginosa PF23 involved in plant growth stimulation, biocontrol and stress amelioration in sunflower under saline conditions. Curr. Microbiol. 69, 484–494 (2014)
Khan, A.A., Shi, Y., Shih, D.S.: Cloning and partial characterization of a b-1,3-glucanase gene from strawberry. DNA Seq. 14, 406–412 (2003)
Shi, Y.: Isolation, characterization and expression analysis of b-1, 3-glucanase genes from strawberry plants. Thesis, Louisiana State University, Louisiana (2005)
Shi, Y., Zhang, Y., Shih, D.S.: Cloning and expression analysis of two [beta]-1,3-glucanase genes from strawberry. J. Plant Physiol. 163, 956–967 (2006)
Khan, A.A., Shih, D.S.: Molecular cloning, characterization, and expression analysis of two class II chitinase genes from the strawberry plant. Plant Sci. 166, 753–762 (2004)
Van Loon, L.C., Van Strien, E.A.: The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins. Physiol. Mol. Plant Pathol. 55, 85–97 (1999)
Benhamou, N., Kloepper, J.W., Quadt-Hallmann, A., Tuzun, S.: Induction of defense-related ultrastructural modifications in pea root tissues inoculated with endophytic bacteria. Plant Physiol. 112, 919–929 (1996)
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Asraoui, M. et al. (2019). Bacillus amyloliquefaciens Enhanced Strawberry Plants Defense Responses, upon Challenge with Botrytis cinerea. In: Ezziyyani, M. (eds) Advanced Intelligent Systems for Sustainable Development (AI2SD’2018). AI2SD 2018. Advances in Intelligent Systems and Computing, vol 911. Springer, Cham. https://doi.org/10.1007/978-3-030-11878-5_5
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DOI: https://doi.org/10.1007/978-3-030-11878-5_5
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