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Phage-Displayed Single Domain Antibodies as Recognition Elements

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Virus Hybrids as Nanomaterials

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1108))

Abstract

The variable domains of antibodies can be expressed as a fusion with pIII, a minor coat protein of the bateriophage M13, for the generation of phage-display antibody reagents. The phage-display system is routinely used to enrich for recombinant antibodies against a specific target antigen from highly diverse naïve and immune libraries. Often once binders are selected, they are expressed as soluble proteins; however, it can be advantageous to use the phage-displayed antibody fragment as a reagent in binding assays. The repeating subunits of the viral capsid allows for significant signal amplification of binding events in downstream assays when utilizing a reporter-conjugated secondary antibody specific for the M13 capsid. Alternatively, labeling of the viral capsid with dyes or biotin molecules provides additional methods of achieving signal amplification in a variety of assay formats. The following protocols detail the use of phage-displayed single domain antibodies in sandwich assays for antigen detection.

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Authors and Affiliations

  1. Naval Research Laboratory, Center for Bio/Molecular Science and Engineering, Washington, DC, USA

    Ellen Goldman & Scott Walper

Authors
  1. Ellen Goldman
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  2. Scott Walper
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Editors and Affiliations

  1. Bio/Molecular Science and Engineering, US Naval Research Laboratory, Washington, District of Columbia, USA

    Baochuan Lin

  2. Bio/Molecular Science and Engineering, US Naval Research Laboratory, Washington, District of Columbia, USA

    Banahalli Ratna

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Goldman, E., Walper, S. (2014). Phage-Displayed Single Domain Antibodies as Recognition Elements. In: Lin, B., Ratna, B. (eds) Virus Hybrids as Nanomaterials. Methods in Molecular Biology, vol 1108. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-751-8_15

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  • DOI: https://doi.org/10.1007/978-1-62703-751-8_15

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-750-1

  • Online ISBN: 978-1-62703-751-8

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