Abstract
Biofilms are adherent communities of bacteria contained within a complex matrix. Staphylococcal species are frequent etiological agents of device-associated biofilm infections in humans that are highly recalcitrant to antimicrobial therapy and alter host immune responses to facilitate bacterial persistence. Here we describe a mouse model of catheter-associated biofilm infection, which can be utilized to investigate the importance of various staphylococcal determinants on disease progression as well as the host immune response to staphylococcal biofilms.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Donlan RM, Costerton JW (2002) Biofilms: survival mechanisms of clinically relevant microorganisms. Clin Microbiol Rev 15:167–193
Stewart PS, Costerton JW (2001) Antibiotic resistance of bacteria in biofilms. Lancet 258: 135–138
Lew DP, Waldvogel FA (1997) Osteomyelitis. N Engl J Med 326:999–1007
NIH Concenus Conference (1995) Total hip replacement. JAMA 273:1950–1956
Garvin KL (1995) Infection after total hip arthroplasty. J Bone Joint Surg Am 77:1576–1588
Morscher E, Herzog R, Bapst R et al (1995) Management of infected hip arthroplasty. Orthop Int 3:343–351
Schoifet SD, Morrey BF (1990) Treatment of infection after total knee arthroplasty by debridement with retention of the components. J Bone Joint Surg Am 72:1383–1390
Rasul AT, Tsukayama D, Gustilo RB (1991) Effect of time on onset and depth of infection on the outcome of total knee arthroplasty infections. Clin Orthop 273:98–103
Burger RR, Basch T, Hopson CN (1991) Implant salvage in infected total knee arthroplasty. Clin Orthop 273:105–111
Hartman MB, Fehring TK, Jordan L et al (1991) Periprosthetic knee sepsis: the role of irrigation and debridement. Clin Orthop 273:113–118
Tsukayama DT, Gustilo RB (1991) Suppressive antibiotic therapy in chronic prosthetic joint infections. Orthopedics 14:841–844
Wilson MG, Kelley K, Thornhill TS (1990) Infection as a complication of total knee-replacement arthroplasty. J Bone Joint Surg Am 72:878–883
Brandt CM, Sistrunk WW, Duffy MC et al (1997) Staphylococcus aureus prosthetic infection treated with debridement and prosthesis retention. Clin Infect Dis 24:914–919
Fitzpatrick F, Humphreys H, O’Gara JP (2005) The genetics of staphylococcal biofilm formation—will a greater understanding of pathogenesis lead to better management of device-related infection? Clin Microbiol Infect 11:967–973
Otto M (2008) Staphylococcal biofilms. Curr Top Microbiol Immunol 322:207–228
Fey PD (2010) Modality of bacterial growth presents unique targets: how do we treat biofilm-mediated infections? Curr Opin Microbiol 13:610–615
Fitzsimmons K, Bamber AL, Smalley HB (2010) Infective endocarditis: changing aetiology of disease. Br J Biomed Sci 67:35–41
Zuluaga AF, Galvis W, Saldarriaga JG et al (2006) Etiologic diagnosis of chronic osteomyelitis: a prospective study. Arch Intern Med 166:95–100
Donlan RM (2001) Biofilms and device-associated infections. Emerg Infect Dis 7: 277–281
Darouiche RO (2004) Treatment of infections associated with surgical implants. N Engl J Med 350:1422–1429
Rupp ME, Ulphani JS, Fey PD et al (1999) Characterization of the importance of polysaccharide intercellular adhesin/hemagglutinin of Staphylococcus epidermidis in the pathogenesis of biomaterial-based infection in a mouse foreign body infection model. Infect Immun 67:2627–2632
Beenken KE, Spencer H, Griffin LM et al (2012) Impact of extracellular nuclease production on the biofilm phenotype of Staphylococcus aureus under in vitro and in vivo conditions. Infect Immun 80:1634–1638
Foster TJ (1996) Staphylococcus. In: Baron S (ed) Medical microbiology. University of Texas Medical Branch at Galveston, Galveston, TX
Herrmann M, Vaudaux PE, Pittet D et al (1988) Fibronectin, fibrinogen, and laminin act as mediators of adherence of clinical staphylococcal isolates to foreign material. J Infect Dis 158:693–701
Rivera J, Vannakambadi G, Höök M et al (2007) Fibrinogen-binding proteins of Gram-positive bacteria. Thromb Haemost 98:503–511
Dunne WM, Burd EM (1993) Fibronectin and proteolytic fragments of fibronectin interfere with the adhesion of Staphylococcus epidermidis to plastic. J Appl Bacteriol 74:411–416
Acknowledgments
This work was supported by the NIH National Institute of Allergy and Infectious Disease (NIAID) P01 AI083211 Project 4 to T.K.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Heim, C.E., Hanke, M.L., Kielian, T. (2014). A Mouse Model of Staphylococcus Catheter-Associated Biofilm Infection. In: Fey, P. (eds) Staphylococcus Epidermidis. Methods in Molecular Biology, vol 1106. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-736-5_17
Download citation
DOI: https://doi.org/10.1007/978-1-62703-736-5_17
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-735-8
Online ISBN: 978-1-62703-736-5
eBook Packages: Springer Protocols