Abstract
Disposable bioreactors have been increasingly implemented over the past ten years. This relates to both R & D and commercial manufacture, in particular, in animal cell-based processes. Among the numerous disposable bioreactors which are available today, wave-mixed bag bioreactors and stirred bioreactors are predominant. Whereas wave-mixed bag bioreactors represent the system of choice for inoculum production, stirred systems are often preferred for protein expression. For this reason, the authors present protocols instructing the reader how to use the wave-mixed BIOSTAT CultiBag RM 20 L for inoculum production and the stirred UniVessel SU 2 L for recombinant protein production at benchtop scale. All methods described are based on a Chinese hamster ovary (CHO) suspension cell line expressing the human placental secreted alkaline phosphatase (SEAP).
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Acknowledgements
We wish to thank: the Fussenegger group (Swiss Federal Institute of Technology Zurich) for engineering the cell line, Nina Steiger and Lidija Lisica (both from the Zurich University of Applied Sciences, School of Life Sciences and Facility Management) for their technical assistance in developing the protocols, and Susanne Roederstein, Tanja Rau, Gerhard Greller, Alexander Tappe (all from Sartorius Stedim Biotech), Sebastian Rothe (GE Healthcare Life Sciences), and Wolfgang Dornfeld (Levitronix) for many helpful discussions and support.
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Eibl, R., Löffelholz, C., Eibl, D. (2014). Disposable Bioreactors for Inoculum Production and Protein Expression. In: Pörtner, R. (eds) Animal Cell Biotechnology. Methods in Molecular Biology, vol 1104. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-733-4_18
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DOI: https://doi.org/10.1007/978-1-62703-733-4_18
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